Electronic Supplementary Material (ESI) for Analytical Methods.

This journal is © The Royal Society of Chemistry 2018

Supplementary data

500

400

300

200

AuM
AuM
100 PtM AuL2 AuL11
PtM AuL AuL1
AuLl AuL PtL2 PtL11
PtLl PtL PtL PtL1 keV
0
0 5 10 15 20

Fig.S1. Fig. S1. EDX spectra of chitosan Au/Pt NCs

1
 1.2

0.8 100 µM
A

200 µM
400 µm
0.6 600 µm
800 µm
1000 µm
0.4

0.2

0
500 550 600 650 700 750 800
Wavelength(nm)

0.9

0.8

0.7
100 µM
A

0.6 200 µM
400 µM
0.5
600 µM
0.4 800 µM

0.3

0.2

0.1

0
500 550 600 650 700 750 800
Wavelength(nm)
a
Fig.S2. Absorbance spectra of catalytic activity of chitosan Au/Pt nanocluster in the presence of

different concentrations of H2O2 and TMB

2
 11
a
0.8
0.8

0.6
0.6
A A

0.4
0.4

0.2
0.2

00
3.2
3.2 4.4
4 5.4
5 66 8.2
8.2
pH
pH

1
0.9
bb
0.8
0.7
0.6
0.6
AA

0.5
0.5
0.4
0.4
0.3
0.3
0.2
0.2
0.1
0.1
0
0
20 25 30 35 40 45 50
20 25 30 35 40 45 50
Temperature (°c)
Temperature (°c)

Fig.S3 Effect of different experimental condition on the peroxidase-like activity of chitosan-

Au/Pt NCs nanostructures: (a) pH, (b), Temperature

3
Fig.S4. FT- IR spectra of chitosan, chitosan Au/Pt nanocluster (C, Red graph) and chitosan Au/Pt
nanocluster Pb2+.

4
Table 1

Determination of Pb2+ ion in milk samples with the proposed method

Sample Added (nM) Found a (nM) Found Recovery()

(ICP-OES)

Milk 100 103 2.0 102.0 103.0

200 2053.0 204.0 102.5

400 406.0 103.0

4122.0
612.0
600 103.8
6234.0
800 106
850.03.0 820.0
1000 1020.0 106.0
1060.03.0

a Results are based on three measurements