Research & Development Department

NFC-Institute of Engineering & Fertilizer Research Faisalabad

Title: Standard Operating Procedure For Titrimetric Analysis

Document/SOP # 2/T2 Date:
Prepared By: Checked & Verified By
Approved By:
1. Purpose
Determination of %CaCO3 & %Ca in a sample of limestone
2. Procedure
2.1. Take one gram of limestone powder sample in a conical flask, digest it with
aqua regia then make its volume one liter in volumetric flask by using
distilled water.
2.2. Take 25 ml sample from 1 liter solution.
2.3. Add 10 ml 1M NaOH & indicator (Calcon) in sample.
2.4. Titrate it with 0.01 M EDTA Solution with blue end point.
2.5. Note used volume of EDTA in ml at the end point.
2.6. Calculate % Ca by using the following formula
F∗Used volume of EDTA∗D
%Ca= ∗100%CaCO 3=%Ca∗2.5
1000∗Sample weight
100 Ml . wt of CaCO
For CaCO ( 40
=
At . wt . of Ca )
=2.5

2.7. Where D = Dilution factor, It is calculated on the basis of take volume and
diluted volume of the solution.
1000
D= =40(¿ this procedure)
25
F= A factor ¿ be calculated as
2.8. Take 5 ml of the standard Ca Solution (1000 ppm) and add 10 ml of 1.0 M
NaOH solution in it with indicator.
2.9. Titrate it with EDTA at Blue end point.
2.10. Now Calculate with factor as
Volume of Ca Standard Solution(5 ml)
F=
Used Volume of EDTA

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