Ding Hou, 1960. The Netherlands, pp.1-48.NEW-Program-Book-5-Oct PDF

PROGRAMME BOOK
1st INTERNATIONAL SYMPOSIUM ON AGARWOOD

AND AROMATIC PLANTS 2019 (1st ISAAP 2019)
“Current Progress of Science and Technology on Agarwod and Aromatic
Plants Production for Conservation and Sustainable Development”
Lombok, October 1 – 2, 2019
ORGANIZED BY:
Faculty of Mathematics and Natural Sciences Mataram University,

Indonesia – CSEAS – The Graduate School and Pharmaceutical Sciences,
Kyoto University, Japan – Local Government Institute West Nusa Tenggara
Province, Indonesia
Supported by:
WELCOME MESSAGE
It is a great pleasure to invite you to Mataram, Lombok – Indonesia to participate in 1st
International Symposium of Agarwood and Aromatic Plants 2019. This year’s symposium will
be an important and comprehensive forum with the purposes:
• To provide a platform for ideas, information exchange, research & publication
collaboration.
• To discuss about commerce, policies and regulations of aglewood species and aromatic
plants.
• To discuss about ecology, environment, conservation, molecular, cytogenetic, anatomy and
biosystematics of aglewood species and aromatic plants.
• To discus aboutinduction and biosynthesis, products development, extraction technologies,
chemical constituents and grading technology, botany, physiology, its symbiont and
pathology of agarwood and aromatic plants.
• To discuss about secondary use, traditional medicine, pharmacological activity of
agarwood and its cell target in the body, ethnobotany of agarwood and aromatic plants.
• To discus about tissue culture, domestication, management of wild and planted agarwood
taxa and planting technology of eaglewood and aromatic plants
• To discuss and evaluate the latest approaches and techniques in agarwood and aromatic
plants research and their production.
• To discuss and evaluate the latest utilization of agarwood and aromatic product from
secondary product.
• To strengthen the international collaboration and networking on agarwood and aromatic
product research and innovation.
We cordially invite you to attend and participate in this significant event.
Sincerely yours,
Dr. Tri Mulyaningsih, M. Si.

Chairperson
1st International Symposium of Agarwood and Aromatic Plants 2019
Faculty of Mathematics and Natural Sciences, Mataram University, Lombok,
West Nusa Tenggara, Indonesia.
1st International Symposium on Agarwood and Aromatic Plants 2019

Golden Palace Hotel Mataram, October 1-2, 2019 2
ORGANIZING
COMMITTEE

PERSON IN CHARGE
Prof. Surya Hadi (Head of Natural Product Chemistry Research Laboratory, Faculty of
Mathematics and Natural Sciences, Mataram University).
SYMPOSIUM ADVISORS
Prof. Yoko Hayami (Director of CSEAS Kyoto University, Japan)
Prof. Emrt. Isamu Yamada (CSEAS Kyoto University, Japan)
Koji Hirose (Director of Graduate School of Pharmaceutical Sciences Kyoto University, Japan)
Prof. Kazuhisa Nakayama (Dean of The Graduate School of Pharmaceutical Sciences, Kyoto
University, Japan)
Prof. Michiho Ito (The Graduate School of Pharmaceutical Sciences, Kyoto University, Japan)
Ir. H. Rosiady Husaenie Sayuti, M.Sc., Ph.D. (Mataram University)

ORGANIZING COMMITTEE
Chairperson : Dr. Tri Mulyaningsih
Secretary : Dr. Kurniasih Sukenti
Nurul Fitriyani, M.Si.
Treasurer : Mamika Ujianita Romdhini, M.Si.

Hj. Suryani Eka W., ST., MBA., Ph.D. (Research &
Development Division, Regional Development Planning
Board, West Nusa Tenggara, Indonesia)
Fundraising : Dr. Ernin Hidayati

Saharudin (Research & Development Division, Regional
Development Planning Board, West Nusa Tenggara,
Indonesia)
Rusmini
Secretarial : Dr. Yuliadi Zamroni

Lale Ratna Tanuri, S.Si.
Program : Dr. Bambang Fajar Suryadi

Dr. Galuh Tresnani
Dr. Irwansyah
Eka Sunarwidhi Prasedya, Ph.D.
Achmad Karyadi Moechson, S. Kom.
Poster : Suripto, M.Si.

Ni Made Sudewianingsih, S.Si.
Angka Sartono, SE.
Publication &
Website : I Wayan Sudiarta, Ph.D.
Consumption : Aida Muspiah, M.Si.

Sri Puji Astuti, M.Si.
Accommodation &
Transportation : Dr. Islamul Hadi
Excursion : Dr. Sarkono Sukiman, MSi.

Hilman Ahyadi, M.Si.
Documentation &
Exhibition : Dr. Rahadi Wirawan

SYMPOSIUM
PROGRAM OVERVIEW

Symposium Schedule
Tuesday, October 1, 2019
08.00 – 09.00 Registration
09.00 – 09.40 Opening Ceremony
09.00 – 09.10 Delivering Indonesian National Anthem (“Indonesia Raya”)
09.10 – 09.20 Committee Report
09.20 – 09.30 Welcome Speech (Rector of Mataram University)
09.30 – 09.40 Welcome Speech and Opening Ceremony
(Governor of West Nusa Tenggara)
09.40 – 09.55 Coffee Break
09.55 – 12.30 Plenary Session I (Keynote Lectures)
Moderator: Prof. I Made Sudarma
09.55 – 10.20 Prof. Michiho ITO
10.20 – 10.45 Prof. S.M.C.U.P. Subasinghe
10.45 – 11.10 Prof. Lina Karlinasari
11.10 – 11.35 Prof. Emeritus Isamu YAMADA
11.35 – 12.00 Dr. Maman Turjaman
12.00 – 12.30 Discussion
12.30 – 13.30 Prayer Time, Lunch Break, and Poster Session
13.30 – 15.15 Plenary Session II (Keynote Lectures)
Moderator: Prof. Surya Hadi
13.30 – 13.55 Assoc. Prof. Yumi Zuhanis Has-Yun Hashim
13.55 – 14.20 Dr. Dadang Wardana

14.20 – 14.45 Dr. Tri Mulyaningsih
14.45 – 15.15 Discussion
15.15 – 15.30 Coffee Break
15.30 – 16.50 Parallel Session
15.30 – 16.50 Garuda Ballroom I (scope)
Moderator: Eka Sunarwidhi Prasedya, Ph.D.
Secondary Use, Phytochemistry, Traditional Medical, Pharmacological

Activity of Agarwood and Aromatic Plants
15.30 – 16.50 Garuda Ballroom II (scope)
Moderator: Dr. Irwansyah
Induction of Agarwood, Commerce, Chemical Constituents of Agarwood

and Aromatic Plants
15.30 – 16.50 Camar Meeting room (scope),
Moderator: Hj. Suryani Eka W., ST., MBA., Ph.D.
Tissue Culture, Ecology, Biosistematic, Management of Wild and Planting

Technology of Eaglewood And Aromatic Plants
16.50 – 17.00 Closing ceremony
19.00 – 21.00 Welcome Dinner (for registered participant only)

MAIN SPEAKER

MAIN SPEAKER
CODE TITLES AUTHOR

MS-01 Regulation and Quota of Agarwood Ir. Wiratno, M.Sc.
& Aromatic Product Plants in
Indonesia
MS-02 Welcome Speech Prof. Dr. Lalu Husni SH., M. Hum
(Rector of Mataram University)
MS-03 Welcome Speech and Opening Dr. Zulkieflimansyah, S.E., M.Sc.
Ceremony
(Governer of West Nusa Tenggara)

MS-01

MS-02
Welcome Speech
(Rector of Mataram University)
Assalamualaikum Warahmatullahi Wabarakatuh
Good morning.
The Honorable:
• Governor of West Nusa Tenggara Province, Mr. Dr. Zulkieflimansyah, S.E., M.Sc.
• Symposium’s Keynote Speakers from various institutions.
• Academic Staffs of Mataram University
• Staffs of Regional Development Planning Board, West Nusa Tenggara Province
• All Guests and Symposium’s Participants
Praise to Allah SWT, the most merciful God, for the knowledge and blessing which have
been given, so that we have this great opportunity to attend this occasion in good condition.
Shalawat and also greetings to Rasulullah Muhammad SAW, the role model of perfect human
being, for all of his efforts, so that we can study in a sophisticated technological era and peaceful
moment like this.
To start with, I would like to warmly welcome the keynote speakers and all participants
of this symposium; it is a great pleasure to have you with us today. It is such kind of honor for
us, Mataram University, to become the organizer of the 1st International Symposium on
Agarwood and Aromatic Plants 2019 (1st ISAAP 2019). This International Symposium is a
collaboration between Mataram University and various institutions, i.e. the Government of West Nusa
Tenggara Province, The Graduate School of Pharmaceutical Sciences and Center of South East Asian
Studies (CSEAS), Kyoto University, Japan. This program represents Mataram University’s effort in
gaining academic insight and confidence in exploring research potential. We hope that this symposium
will be able to transfer positive energy to every lecturer, student, and staff in Mataram University to
perform professionalism in teaching, research, and community development at national and even
international level.

Ladies and Gentlemen,
Mataram University has the vision to become an international research-based university

for 2025. Therefore, in achieving it, Mataram University consistently develop and make serious
efforts in: 1) Executing research-based higher education process with national and international
standard quality in order to produce religious, virtuous, and competent human resources with
global insight; 2) Executing research activities with national and international standard quality
to produce advance technology as new science references, and problem solver in society in
order to support excellent learning process as well as community development; and 3)
Developing a wide-range networking with various national and international institution,
governmental institution and private sector, in order to support the implementation of teaching,
research, and community development with national and international quality.
As an implementation of the aforementioned goals, Mataram University has been

established a Center of Excellent for Science and Technology with agarwood as the research
core. Agarwood becomes research focus since agarwood has wide range species with high
economic value. West Nusa Tenggara is one of the province in Indonesia that succeed in
agarwood cultivation, it has been proven with the growth of agarwood cultivator in several
locations in Lombok and Sumbawa. However, agarwood utilization as featured products as well
as the number of international publication about agarwood is still limited. I strongly believe that
through this symposium, Mataram University can become a research center of agarwood and
develop sophisticated technology for agarwood utilization, thus the number of international
publication will consistently increase.
As mentioned before, agarwood not only has research potential but also has high
economic value. Therefore, the research program in the Center of Excellent for Science and
Technology of Agarwood must be able to produce a featured product that capable to compete
at 4.0 industrial era. Until now, the Center of Excellent for Science and Technology of
Agarwood has been developed some featured product, for example: drugs, aromatherapy,
perfume, cosmetic, sensor, and many more. We greatly expect this symposium can transmit a
positive atmosphere for our researcher to invent more innovative research program and
encourage Mataram University to become a research-based university with international
quality.

Finally, once again I would like to convey the warmest welcome to all participants of this
symposium. I hope that we always intertwine a harmonious and synergic relation on the chance
to encourage research growth as well as science and technology development in Indonesia.
Hopefully, may Allah SWT bless us and this symposium. Aamiin yaa Robbal Alaamiin.
Thank you for your attention.
Wabillahi Taufiq Wal Hidaayah Wassalamu'Alaikum Warohmatullohi Wabarokaatuh
Mataram, October 1, 2019

Rektor University of Mataram,
Prof. Dr. Lalu Husni S. H., M. Hum.

MS-03
Welcome Speech and Opening Ceremony
(Governer of West Nusa Tenggara)
Assalamu’alaikum warahmatullahi wabarakatuh
Greetings, and best wishes to all of us,
Alhamdulillah, let us always pray and praise our gratitude to the presence of Allah Almighty
God, because of His grace we can attend and enliven the 1st International Symposium on
Agarwood and Aromatic Plants. Blessings and greetings may always be devoted to the great
Prophet Muhammad SAW because He is a great role model for mankind in navigating our life
in this world.
Ladies and Gentlemen, let me start this speech by saying welcome to the keynote speakers,
moderators and participants in West Nusa Tenggara (NTB), especially in this great and
hopefully blessing place. On behalf of the NTB provincial government and personally, I would
like to give a big appreciation to the Mataram University (UNRAM) in organizing this
symposium. This event will be a great and right moment for researchers and NTB young
generation to develop and enhance their knowledge and skills in research and industry in terms
of developing our region.
NTB is focusing on the development of the industrial sector both tourism and economic
empowerment. And agarwood is one of the best commodities from the local that can be
excellent in the industrial. Agarwood can be found growing in the wild and now agarwood is
one of the cultivation businesses with large turn over because of its high economic value. Its
raw materials and its products from it such as perfume and cosmetics are commodities in NTB.
Even, the agarwood product is exclusive in several countries such as in Middle East. Agarwood

also can attract tourists to come to NTB, for instance, they can travel to agarwood gardens.
Furthermore, NTB makes the tourism industry as a priority development to increase

regional income. All in all, there is a big commitment from the government and entrusts NTB
as the host of international events at Special Economic Zone (KEK).
Ladies and gentlemen,
However, there is agarwood exploitation because of its value, which makes these plants
become an endangered plant (according to the Convention on International Trade in
Endangered Species of Wild Fauna and Flora (CITES)). Therefore, the NTB Government in
collaboration with UNRAM created a Center of Excellent for Science and Technology focussed
on agarwood. There are some objectives that we want to achieve. First of all, the initiation for
agarwood cultivation; and the second is developing and invent a new technology that can be
used to improve the quality and productivity of agarwood products.
Currently, NTB has succeeded in conducting agarwood cultivation which can be seen by
the increase number of farmers and entrepreneurs who cultivate agarwood. But on the other
hand, still, need more progress in developing and inventing the technology, as well as finding
the right market place for the products.
So, to overcome some of these problems, intensive research is needed to upgrade product
quality. As well as, in making and developing an Excellent Agarwood Research Center in
technology. Of course, to achieve these, it is necessary to hold cooperation from various
national and even international institutions. So that the realization of technology that can
improve the quality and productivity of agarwood processed. This is certainly an effort and
expected to increase the welfare of the people of NTB.
Once again, industry is a priority sector in developing our region. So, we expect that
agarwood can be an excellent commodity in both tourism and economic empowerment
industry. Through this event, hopefully, agarwood products in NTB can be widely known both
nationally and internationally. Through this event, we can share knowledge and experiences, so
that we can modify and apply science and technology to solve problems and improve the quality
of our research. As well as, we can establish an international cooperation. Once again, welcome

to NTB, and finally, by saying: "Bismillaahir Rahmaanir Rahiim", the 1st International
Symposium on Agarwood and Aromatic Plants symposium is OFFICIALLY OPENED. May
this event will be blessed by Allah SWT.
Thank you for your attention
Wassalamu 'Alaikum Warahmatullaahi Wabarakaatuh.
Mataram, October 1, 2019

Governor of West Nusa Tenggara,
Dr. Zulkieflimansyah, S.E., M.Sc.

KEYNOTE SPEAKER

KEYNOTE SPEAKER
CODE KEYNOTE SPEAKER TITLES AUTHOR

KS-01 Pharmacological Activities of Michiho Ito
Agarwood Fragrance.
KS-02 Agarwood Resin Inducement in S.M.C.U.P. Subasinghe
Gyrinops Walla: Beyond Fungal Hitihamu, I.
Inoculations Withanage, S.
Malithi, R.A.P.
Vidurangi, A.N.K.G
KS-03 Technology for detecting agarwood: Lina Karlinasari
acoustics, electrical impedance and
NIR spectroscopy
KS-04 Agarwood connection in tropical Asia Isamu Yamada
KS-05 Effects of Agarwood at Multiple Yumi Zuhanis Has-Yun Hashim
Stages of Carcinogenesis: A Nurhusna Samsudin
Systematic Review Nazurah Ab llah
Ummu Athiyah Mohammad
Tormizi
Asmak Muti’ah Mohd Nasir Nur
Umisha Zainudin
KS-06 Bio-Induction Technology of Maman Turjaman
Agarwood and Aromatic Product Asep Hidayat
Plants in Indonesia
KS-07 Biodiversity, Biography and Tri Mulyaningsih

Conservation of Gyrinops Species Isamu Yamada
(Thmelaeaceae) In Indonesia Michiho Ito

ORAL PRESENTATION

ORAL PRESENTATION TITLES
GARUDA BALLROOM I, LT. 3

SECONDARY USE, PHYTOCHEMISTRY, TRADITIONAL MEDICAL,
PHARMACOLOGICAL ACTIVITY OF AGARWOOD AND
AROMATIC PLANTS
ORAL PRESENTATION
CODE AUTHOR
TITLES
OP-S1 The Effects of Aquilaria Faridah Ismail, Azantee Yazmie
malaccensis Leaves Abdul Wahab, Muhammad Lokman
Aqueous Extract on Sperm Md Isa,
of Sprague Dawley Rats Hussin Muhammad, Raja Arif Shah
towards Early Raja Ismail, Redzuan Nul Hakim
Embryogenesis. Abdul Razak, Muhammad Alif
Mazlan
OP-S2 A Review of Secondary Surya Hadi, Baiq Desy Ratnasari,
Metabolites of Gyrinops Tri Mulyaningsih, Kurniasih Sukenti,
versteegii (Gilg.) Domke for Yuliadi Zamroni, Michiho Ito, Isamu
Drugs and Cosmetic Yamada
Material Purposes
OP-S3 Profiling of Compounds in Natasha Jafar Ali,
Hydrosol Extract of Yumi Zuhanis Has-Yun Hashim,
Aquilaria (Agarwood) Nur Aimi Aliah Zainurin
Species using Gas
Chromatography-Mass
Spectrometry
(GCMS).
OP-S4 The Effect of Aquilaria Redzuan Nul Hakim Abdul Razak,
malaccensis Leaves Muhammad Lokman Md Isa,
Aqueous Extract on Male Hussin Muhammad
Reproductive System of
Sprague Dawley Rats:
Sperm Quality and
Testicular Histological
Findings.
OP-S5 GC-MS Analysis and Najihah Mohd Noor,
Antibacterial Activity of Yumi Zuhanis Has-Yun Hashim,
Aquilaria malaccensis Leaf Ummu Athiyah Mohammad Tormizi,
Extract. Asmak Muti’ahMohd Nasir, Nur

Umisha Zainudin
OP-S6 Agarwood Prescription in Nurulhusna Samsudin, Yumi Zuhanis
Malay Traditional Medical Has-Yun Hashim, Mohammad
Manuscripts. Tormizi, UA., Mohd Nasir, AM.,
Zainudin, NU.
GARUDA BALLROOM II, LT. 3
INDUCTION OF AGARWOOD, COMMERCE, CHEMICAL
CONSTITUENTS OF AGARWOOD AND AROMATIC PLANTS
ORAL PRESENTATION
DE AUTHOR
TITLES
OP-I1 The Best Inoculation Resti Wahyuni
Technique Applied on Amalia Indah Prihantini
Gyrinops versteegii Tree
Trunk at Height of 4-5
meters.
OP-I2 Bioinduction of agarwood in Ahmad Faizal, Alda Wydia Prihartini
cultivated Gyrinops Azar, Maman Turjaman
versteegii (Gilg.) Domke
using Fusarium solani
OP-I3 The Effectiveness of Gaharu Lutfi Anggadhania L., YMM Anita
Induction by Fungal Nugraheni, Nurul Wahyuni
Inoculationon Gyrinops
OP-I4 History of the Agarwood of Hussain Ahmed Al Rifaei
the Arabs
OP-I5 Eaglewood Cultivation and Sudirman
Marketing in West Nusa
Tenggara Barat
OP-I6 Morphological and Gowth Ernin Hidayati, Tri Mulyaningsih,
Characteristics of Faturrahman, Sarkono, Nilam
Endophytic Phomopsis sp. Fadmaulidha Wulandari, Michiho
and Aspergillus sp. from Ito, Isamu Yamada
Gyrinops versteegii
OP-I7 Nutrient Injection for Supriyanto
Agarwood Formation
CAMAR MEETING ROOM LT. 2

TISSUE CULTURE, ECOLOGY, BIOSISTEMATIC, MANAGEMENT
OF WILD AND PLANTING TECHNOLOGY OF EAGLEWOOD AND
AROMATIC PLANTS

ORAL PRESENTATION
CODE AUTHOR
TITLES
OP-T1 Elevating Agarwood Erna Listiana, Sumarjan, Ferdinand
Fragrance Sesquiterpenes Schroeder, Ulrich Schurr, Tri
Production in Aquilaria Mulyaningsih
filaria Suspension Cultures
OP-T2 Production of agarwood Agus Sukito, Cecep Handoko
constituents by callus
cultures of Gyrinops
versteegii (Gilg.) Domke.
OP-T3 Development of Agarwood Yulizah, Agung Adi Nugroho, Joeni
Producing Tree in Riau Setijo Rahajoe
Province (Case Study:
Agarwood Plantation in
Siak Sri Indrapura District
and Kampar District).
OP-T4 Ecological and Economical Thifali Adzani, Koji Matsushita,
Assessment of Agarwood Mamoru Kanzaki
Plantation in Central
Bangka, Indonesia
OP-T5 Phylogenetic Study of the Marlina Ardiyani, Lulut D.
Indonesian Agarwood using Sulistyaningsih, Wita Wardani,
Nuclear and Chloroplast Kusumadewi S. Yulita,, Lina S.
DNA Juswara, Seni K. Senjaya, Rani
Asmarayani, Susila.
OP-T6 Strengthen Farmer on Rubangi Al Hasan, Amalia Indah
Agarwood BMW Marketing Prihantini, Mansyur
Chain.

POSTER
PRESENTATION

POSTER TITLES
BIODIVERSITY, BIOSYSTEMATICS, ANATOMY,

ETHNOBOTANY, ITS SYMBIONT OF AGARWOOD AND
AROMATIC PLANTS
CODE POSTER TITLES AUTHOR
P-E1 Species Identification of Agarwood using a Seiji Tanaka
DNA Barcoding Method Michiho Ito
P-E2 Species of Eaglewoodweed (Cyperaceae) Wiwin Hidayat
in Mother Plants Garden of Eaglewood Tri Mulyaningsih
Pusuk, West Lombok, Indonesia
P-E3 Stem Anatomical Variation of the Genus Karmilawati
Gyrinops (Thymelaeaceae) in Sulawesi, Tri Mulyaningsih
Indonesia. Aida Muspiah
Michiho Ito
P-E4 Diversity of Ferns in under the Stand of Lukmanul Hakim
Gyrinops versteegii in the Mother Plants Ni Nengah Sri Eni
Garden of EaglwoodPusuk, West Lombok, Tri Mulyaningsih
Indonesia.
P-E5 Comparative of Stem Anatomy in Genus Zainul Muttaqin Sany
Gyrinops (Thymelaeaceae) on the Tri Mulyaningsih
Sumbawa Island Aida Muspiah
Isamu Yamada
Michiho Ito
P-E6 Identification of Epiphytic Ferns Living on Laily Hunawatun Sani
Gyrinops versteegii in Parental Garden of Janwar Wahyu
Eaglewood Pusuk, West Lombok. Tri Mulyaningsih
P-E7 Diversity of Aromatic Plants Via Febrianti
(Zingiberaceae) in Mother Plants Garden Ainun Astrid
of Eaglewood Pusuk Tri Mulyaningsih
West Lombok Indonesia.
P-E8 Diversity and Feeding Ecology of Yuliadi Zamroni
Herpetofauna at Eaglewood Gyrinops Ahmad Jupri
versteegii Conservation Forest of Pusuk, Baiq Farista
Western Lombok. I Gede Nano Septian
Surya Hadi,
Tri Mulyaningsih
Michiho Ito
Isamu Yamada
P-E9 Spiders Diversity at Lalu Arya Kasmara
EaglewoodGyrinopsversteegiiConservation I Wayan Suana
Forest of Pusuk, Western Lombok. Yuliadi Zamroni

P-E10 The utilization of Gyrinops versteegii (Gilg.)
Kurniasih Sukenti
Domke on Lombok and Sumbawa Island: an Sukiman
ethnobotanical perspective. Immy Suci Rohyani
Tri Mulyaningsih
Surya Hadi
Michiho Ito
Isamu Yamada
P-E11 Diversity of Bryophyta Associated with Vira Heryanto
Gyrinopsversteegiiin Mother Plants Garden of Kartini Ambarwati
Eaglewood Pusuk West Lombok Indonesia Fitri Ramdani
Haerunnisa
Tri Mulyaningsih
AGARWOOD INDUCTION AND GRADING TECHNOLOGY
OF AGARWOOD AND AROMATIC PLANTS
P-I1 Comparison of constituents in artificial Sakura Takamatsu
agarwood pieces made by different methods. Michiho Ito
P-I2 Design of Electronic Nose for Classification Farel Ahadyatulakbar
Agarwoodof Gyrinops versteegii Aditama
Rahadi Wirawan
Laili Mardiana
Tri Mulyaningsih
Nurul Qomariyah
P-I3 Antioxidant Activity of Agarwood Produced Amalia Indah Prihantini
after Repeated Inoculation on Resti Wahyuni
Gyrinopsversteegii (Gilg.) Domke
P-I4 Study of Volatile Compound from The Nail Ahmad Teguh Riady
Wounded Trees Producing Agarwood Ni Komang Tri Dharmayani
(Gyrinopsversteegii (Gilg.) Domke) Michiho Ito
Isamu Yamada
Surya Hadi
P-I5 An Investigation of volatile compound from Muhammad Rizki Juwardi
the nail-wounded-trees producing agarwood Murniati
Gyrinops versteegii based on GC- MS. Michiho Ito
Isamu Yamada
Surya Hadi
PHYTOCHEMISTRY, MEDICINE USED, PHARMACOLOGICAL

ACTIVITY OF AGARWOOD AROMATIC PLANTS
P-P1 Toxicity Test of Agarwood (Gyrinops Jeksi Mahari Nurpratama
versteegii (Gilg.) Domke) Leaf Methanol Emmy Yuanita
Extract Using Brine Shrimp Lethality Test Michiho Ito
(BSLT). Isamu Yamada
Surya Hadi

P-P2 Toxicity Test of the Methanol Extracts of Dian Lestari
the Stem Bark of Agarwood (Gyrinops Ni Komang Tri Dharmayani
versteegii (Gilg.) Domke) as an Anticancer Michiho Ito
Agents Using the Brine Shrimp Lethality Isamu Yamada
Test (BSLT). Surya Hadi
P-P3 Antioxidant Activity Test of Methanol Fitra Fauhan Rahmasari
Extract of Agarwood Leaves (Gyrinops Ni Komang Tri Dharmayani
versteegii (Gilg.) Domke) Using DPPH Michiho Ito
Method. Isamu Yamada
Surya Hadi
P-P4 Rosmarinic acid comparison between Perilla Yuya Deguchi
species. Michiho Ito
TISSUE CULTURE OF EAGLEWOOD AND AROMATIC PLANTS

P-T1 Methyl Jasmonate and Crude Extract of Ahmad Faizal
Fusarium solani elicit agarwood compounds Rizkita Rachmi Esyantia
in shoot culture of Aquilaria malaccensis Nisaa Adn’aina
Lam. Silmi Rahmania
Alda Wydia Prihartini Azara
Maman Turjaman
P-T2 Secondary Metabolite Profiles ofAquilaria Ahmad Faizal
malaccensis Lamk. Callus Culture Elicited Rizkita Rachmi Esyanti
with Fusarium solani MYCELIUM Extract Nadia Puji Utami
and Methyl Jasmonate. Maria Masitho Makajanma
Maman Turjaman
P-T3 Distribution Mapping of Agarwood Nurul Fitriyani
Plantation in Lombok Island Muhammad Sopian Sauri
Mamika Ujianita Romdhini
Agus Kurnia
Surya Hadi
Tri Mulyaningsih
Michiho Ito Isamu Yamada

ABSTRACT
KEYNOTE SPEAKER

KS-01
Pharmacological Activities of Agarwood Fragrance
Michiho Ito
Graduate School of Pharmaceutical Sciences, Kyoto University 46-29, Yoshidashimoadachi, Sakyo,

Kyoto 606-8501 Japan
Corresponding author E-mail: michihoi@pharm.kyoto-u.ac.jp
Abstract
Scientific studies on agarwood have been greatly advanced recently, and many papers on methods
for inducing oleoresins in tree trunks, phylogenetic relationships and species identification of relevant
species using sequencing techniques, or reviews on those papers were frequently published in many
types of scientific journals. Those increasing number of papers indicates that many people irrelevant to
their nationalities nor their scientific backgrounds are interested in agarwood.
The reason why so many people are interested in agarwood may lie in its peculiarity and rarity as
fragrant wood; however, it has long been used not only as aromatic material but as herbal medicine that
acts as sedative. Does this mean the smell of agarwood have sedative activity?
In order to answer this question, pharmacological studies using mice experiment systems were
performed for volatile fraction of heated agarwood [1-6]. Simultaneously, phytochemical analyses of
agarwood smell were performed on GC-MS and on HPLC to elucidate active principles [7].
Characteristic sesquiterpenes and low-molecular-weight- aromatic compounds were found to have
strong sedative activities.
Some more findings and insights on fragrant compounds with activities will be discussed in the
lecture.
Keywords: agarwood, sedative, pharmacology of smell
References:
[1] Hiroaki Takemoto, Michiho Ito, Tomohiro Shiraki, Toru Yagura, Gisho Honda, J. Nat. Med., 62
(1), 41-46 (2008).
[2] Koichi Kishida, Michiho Ito, Shoyakugaku Zasshi, 63 (2), 39-45 (2009).
[3] Takashi Miyoshi, Michiho Ito, Takashi Kitayama, Sachiko Isomori, Fumiyoshi Yamashita, Biol.
Pharm. Bull.36 (9), 1474-1481 (2013).
[4] Yumi Fujiwara, Michiho Ito, Planta Medica, 81 (3), 193-199 (2015).
[5] Kakuyou Ogawa, Michiho Ito, Biol. Pharm. Bull., 39, 794-798 (2016).
[6] Kakuyou Ogawa, Michiho Ito, Planta Medica, 82, 84-88 (2016).
[7] Sakura Takamatsu, Michiho Ito, J. Nat. Med.,72 (2), 537-541 (2018).

KS-02
Agarwood Resin Inducement in Gyrinops Walla:
Beyond Fungal Inoculations
Subasinghe, S.M.C.U.P., Hitihamu, I., Withanage, S., Malithi, R.A.P., Vidurangi, A.N.K.G.
Centre for Forestry and Environment, Department of Forestry and Environment, University of Sri
Jayewardenepura, Nugegoda, CO 10250, Sri Lanka
Corresponding author E-mail: upuls@sjp.ac.lk
Abstract
Gyrinopswalla is the only naturally growing agarwood producing tree species in Sri Lanka. It had
no commercial recognition until its agarwood producing ability was scientifically identified in 2012.
This paved avenues for numerous researches. This paper illustrates some studies conducted on different
inoculation methods using fungal species and their substances.
The first attempt was made in 2014 to identify the potential fungal species that can be used at
commercial scale for agarwood formation in G. walla. The inhabiting fungal species were identified
from naturally formed agarwood tissues G. wallatrees of the wet zone of Sri Lanka. Those tissues
were size reduced, surface sterilised and placed in agar media to grow the inhabiting fungi which
were then isolated and identified with colony and morphological characteristics. Altogether, 4
Fusarium, 4 Trichoderma, 3 Aspergillus, 2 Botryosphaeria species and 1 species of each of
Diplocladium, Mucor and Sarcinomyces were identified. When re-inoculated as pure cultures, A.
nigerand F. solaniproduced the highest agarwood resin contents (0.82±0.07% and 0.73±0.06%
respectively) in G. wallaup to 50 cm above the inoculation point in 6 months. 16 and 15 key resin
constituents were identified from those resins formed by A. nigerand F. solanirespectively. Once 100
ml of spore suspensions of A. nigerand F. solaniin nutrient broth media were inoculated, the resin
contents were 0.37±0.06% and 0.40±0.18% respectively after 4 months with pale yellow to brown in
colour. Then potential of agarwood inducement using secondary metabolites was tested using different
strains of the same fungal species. For this reason, the above two fungal strains (ASP-U and FUS-
U) and two other strains (ASP-N of A. nigerand FUS-N of F. solani) were grown in agar media and
their secondary metabolites were extracted by filtering through a series of ceramic filters of
descending pore sizes. A bioassay conducted on G. wallaleaves confirmed the toxicity of secondary
metabolites only for ASP-U and FUS-U strains. Then, 50 ml of those were inoculated to G. wallaand
aftter 7 months, only ASP-U and FUS-U which were obtained from the naturally formed agarwood
produced resins while the other two strains were not productive. ASP-U produced resins (2.27±0.39%)
only up to ±20 cm from the inoculation point while FUS-U produced resins (4.21±0.47%) up to
±60 cm. The oil colour was yellow with 14 key constituents and dark yellowish brown with 24 key
constituents for ASP-U and FUS-U respectively. The results lead to assume that only some strains of
the same fungal species are capable of inducing agarwood. Secondary metabolites of the effective
fungal species can also be used for agarwood inducement which can be chemically manufactured at
commercial scale.
Keywords: Gyrinopswalla, Fusarium solani. Aspergillus niger, Fungal strains, Agarwood

Figure 1: Agarwood tissues formed by FUS-U and ASP-U strains after 7 months.

KS-03
Technology for Detecting Agarwood:
Acoustics, Electrical Impedance, and Nir Spectroscopy
Lina Karlinasari
Department of Forest Products, Faculty of Forestry, IPB University IPB Kampus Darmaga, Bogor 16680, West
Java, Indonesia
Corresponding author E-mail: karlinasari@apps.ipb.ac.id
Abstract
As a high commercial value commodity, agarwood or gaharu tree can be extracted from various
parts of the tree. For that reason, agarwood tree has been heavily exploitated, particularly from genus of
Aquilaria and Gyrinops (Tymelaeaceae). The woody part of the tree especially heartwood is the most
sought due to its unique fragrances as a response to the injury in which fungus attack on the tree causes
physiological and chemical changes in the wood. Many research have been carried out on how the
mechanism occurs as well as tree improvement and production. However, research to detect and confirm
the presence of agarwood is still limited that may cause current gaharu harvest unsustainably due to trial
and error practices. Technology for non-destructive testing (NDT) carried out as from the standing trees
can be applied to detect and predict the existence of agarwood. Traditional knowledge based on visual
and natural signs has become the important step to determine the presence of agarwood that can be
complemented by technology to ensure the inside condition based on parameters detected. Technology
based on the measurement of sound wave velocity as well as electrical impedance parameter to assess
internal stem or wood characteristics has been developed in which those parameters will differ
depending on the density or other heterogeneous characteristics of wood in the tree. Other technologies
also have been developed to provide a rapid detection methods of wood property assessment. Near-
infrared (NIR) spectroscopy have been used widely to identify wood properties (e.g. chemical contents)
and the origin of agarwood.
Keywords: gaharu, NDT, nondestructive, sound velocity, NIR spectroscopy
References:
[1] Indahsuary N, Nandika D, Karlinasari L, Santoso E. Journal of the Indian Academy Wood Science,
11(1): 65-71 (2014).
[2] Karlinasari L, Sabed M, Wistara INJ, Purwanto YA. Journal of the Indian Academy Wood Science,
Vol. 11(2): 162-167 (2014).
[3] Karlinasari L, Indahsuary N, Kusumo HT, Santoso E, Turjaman M, Nandika D. Journal of Tropical
Forest Science,27 (3):351-356 (2015).
[4] Karlinasari L, Putri N, Turjaman M, Wahyudi I, Nandika D. Turkish Journal of Agriculture and
Forestry,40 (5): 696-704 (2016).
[5] Karlinasari L, Nandika D. Chapter 9 ”Acoustic-Based Technology for Agarwood Detection in
Aquilaria Trees” in Agarwood: Science behind the Fragrance. Mohamed R, Ed, Springer Verlag,
pp. 137-148, (2016).
[6] Putri, NKarlinasari L, Turjaman M, Wahyudi I, Nandika D. Agriculture and Natural Resources,51:
84-90 (2017).

KS-04
Network on Agarwood Collectors in Borneo 1990’s
Yamada Isamu
Center for Southeast Asian Studies, Kyoto University

Corresponding author E-mail: yamada@cseas.kyoto-u.ac.jp
Abstract
From 1965 onward, I have been working in tropical rainforest in Southeast Asian as a forest
ecologist. During staying in the forest, extensive researches on Agarwood were carried out. Almost all
the areas of Brunei, Kalimantan and Sabah, Sarawak in Borneo island were surveyed. Brunei is a unique
small country where 3 years were spent as a JICA expert in 1984 to 1986. During that time, forest
ecological studies in the peat swamp, Agathis and mixed dipterocarp forests were intensively carried
out. The first agarwood was measured in my permanent plot in K7, Andulau Burunei.
In Kalimantan, extensive fieldworks along major rivers such as Kayan, Barito, Mahakam and
Kapuas were carried out from the mouth of rivers to the top of the inner mountains. I met many agarwood
collectors in the forest and remote villages and interviewed how they are working in the forests. Similar
studies were done in Sarawak and Sabah in 1990’s. Overseas Chinese merchants who are living in the
local middle cities such as Tarakan, Miri, Balikpapan, Samarinda, Malinau and Marudi were also
interviewed to get information how to collect agarwood materials. The biggest market was Singapore,
at that time. Agarwood merchants in Ruby warehouse were visited many times to know the networks
between original production areas to Arab countries, China, Taiwan and Japan.
In this paper, agarwood trade network are shown based on the fieldworks in the last decade of 20
century.
Keywords: Borneo, Penan, Kalimantan, trade, network.
References:
[1] Yamada, “Aloeswood Forest and The Maritime World, ”Southeast Asian Studies, 33(3): 463-468,
(1995)
[2] Yamada, Tropical rain forests of southeast Asia : A Forest Ecologist’s View(trans. by Peter
Hawkes). University of Hawaii press, pp.392, (1997).
[3] Yamada, “Eco Links for the Conservation of Important Eco Resources in Indonesia,” in Proceeding
BILATERAL SYMPOSIUM Technology the rules of Mathematics and Sciences for Sustainable
Development, Mataram, Indonesia, (2017).
[4] Yamada, J. Akamine, M. Hirata(eds), Eco resources: The World to make material, place and people
alive, Showado, pp.277, (2018).
[5] Yamada, “Considering Eco resources”in Eco resources: The World to make material, place and
people alive, I. Yamada, J. Akamine, M. Hirata (eds),Showado, p.1-16,(2018).

KS-05
Effects of Agarwood at Multiple Stages of Carcinogenesis:
A Systematic Review
Yumi Zuhanis Has-Yun Hashima, Nurhusna Samsudina, Nazurah Ab llaha*, Ummu Athiyah
Mohammad Tormizib, Asmak Muti’ah Mohd Nasirc, Nur Umisha Zainudind
aInternational Institute for Halal Research and Training (INHART), International Islamic University Malaysia
(IIUM), Jalan Gombak, 53100 Kuala Lumpur, Malaysia.
bBiotechnologyProgramme, Institute of Biological Sciences, Faculty of Science, University Malaya,
Jalan Universiti, 50603, Kuala Lumpur, Malaysia

cBiochemistryProgramme, Institute of Biological Sciences, Faculty of Science, University Malaya,

dDepartment of Biomedical Sciences, Kuliyyah of Allied Health Sciences, International Islamic University
Malaysia (IIUM), 25710, Kuantan, Malaysia
Corresponding author e-mail: yumi@iium.edu.my
Abstract
This systematic review sought to determine whether preclinical biological and mechanistic
evidences of agarwood materials reported at multiple carcinogenic stages are significant to support
plausibility of agarwood materials as potential therapeutics for cancer. Agarwood materials have been
reported to have pre-clinical (in vitro and in vivo) evidences of anti-cancer effects in various types of
cancer, however the diverse range of types of agarwood species and materials as well as carcinogenesis
stage pose a challenge to discern the potential candidate or scheme for further work. More recent reports
on agarwood toxicity further complicates the search for the potential therapeutics. Study selection
criteria was determined based on framework outlined by the World Cancer Research Fund (WCRF)
International/University of Bristol (UoB). In addition, care was taken to follow PRISMA reporting
guidelines as closely as possible. Anti-cancer effect in the studies must be related to properties of
antigenotoxic, apoptosis, antioxidant, cytotoxic, anti-inflammatory, anti- attachment, anti-spreading,
anti-metastatic and anti-angiogenic. Papers that are published other than in English; review paper,
comments and opinions; short paper (less than 6 pages), poster keynote and abstract; and papers
published in 1900 and below are excluded. Search strategy was applied to selected electronic databases
including Scopus, Springerlink, Taylor & Francis Online, Cambridge Core, Pub MEDLINE, Oxford
Academic, Science Direct and Wiley Online Library. Number of 4473 studies were initially identified
and 860 studies were included following screening. After removing duplicates, 112 papers were further
analysed for eligibility.
Presently, 47 studies judged eligible. Interim findings showed several characteristic trends where
type of materials studied ranges from leaf, branch and essential oil; species studied are region/country
specific but types of cancer studied are not research group-specific. Initial conclusion from the
preclinical studies could be made where different species and types of agarwood material may have
effects at multiple carcinogenesis stage in various types of cancer.
Keywords: agarwood, systematic review, anti-cancer, PRISMA, carcinogenesis

References:
[1] Hashim, Y.Z.H-Y., Kerr, P.J. Abbas, P and Mohd Salleh, H.Aquilaria spp. (agarwood) as source
of health beneficial compounds: a review of traditional use, phytochemistry and pharmacology.
J.Ethnopharmacol. 189, 331-360.
[2] [3] Hooijmans, C.R.; Rovers, M.M.; de Vries, R.B.M.; Leenaars, M.; Ritskes-Hoitinga, M.;
Langendam, M.W. (2014). SYRCLE’s risk of bias tool for animal studies. BMC Med. Res.
Methodol. 14, 43.
[3] [2] Lewis, S.J.; Gardner, M.; Higgins, J.; Holly, J.M.P.; Gaunt, T.R.; Perks, C.M.; Turner, S.D.;
Rinaldi, S.; Thomas, S.;Harrison, S.; et al. (2017). Developing the WCRF International/University
of Bristol methodology for identifyingand carrying out systematic reviews of mechanisms of
exposure-cancer associations. Cancer Epidemiol. Biomark. Prev. 26, 1667–1675.
[4] [4] Moher D, Liberati A, Tetzlaff J, Altman DG, The PRISMA Group (2009). Preferred Reporting
Items for Systematic Reviews and Meta-Analyses: The PRISMA Statement. PLoS Med 6(7).

KS-06
Bio-Induction Technology of Agarwood and Aromatic Product Plants
in Indonesia
Maman Turjamana* and Asep Hidayata
aForest Microbiology Research Group, Forest Research and Development Centre, Forestry and Environment
Research, Development, and Innovation Agency, The Ministry of Environment and Forestry, Jalan Gunung Batu
No. 5, Bogor, Indonesia 16680
Corresponding author E-mail: turjaman@gmail.com
Abstract
Bio-induction technology based on microbe is an eco-friendly and reasonable technology for
agarwood sustainable production to substitute wild agarwood high demand in international trading.
Nowadays, many agarwood production trees planted find everywhere by farmer groups, but agarwood
inoculant supply becomesa bottle neck for scaling up agarwood production in Indonesia.
Based on some results of analysis in laboratory, anti-oxidant and anti-fungal activity were examined
from agarwood cultivated. There were 50 endophytic fungi successfully isolated from Aquilaria
malaccensis, A. microcarpa, A. crassna, and Gyrinops versteegii. Apodus Oryzae and Diaporthe sp.
exhibited strong antioxidant activity. Pestalotiopsis theae and Curvularia sp. showed antifungal activity
against Fusarium solani. Agarwood from A. malaccensis and G. versteegii constituent compounds have
identified and analysis by GC-MS from seedlings and trees after inoculation, but more were deposited
in agarwood trees. Agarwood formation can be artificially stimulated by fungal induction. It means that
bioinduction promotes artificially induced agarwood formation in Aquilaria and Gyrinops in terms of
color, aromatic odor, and chemical constituents.
There isa strategy to increase sustainable agarwood production in Indonesia. It is possible local
government should make a model of agarwood production cluster with stakeholders in provincial level.
The mass production of inoculant of agarwood should be provided by government or private company
collaboration with research institute or national university, doingbenefit sharing cooperation with farmer
groups. The success level of agarwood cultivated product depends on effectiveness of fungal inoculant,
knowledge of farmers for doing inoculation process, and environment factors. The key factor that may
support sustainable agarwood productions are simple government regulation and marketing especially
for agarwood cultivated. They should have plan and support integrated facilities and design of‘one stop
services’ for agarwood industry in Indonesia.
Keywords: Aquilaria, Gyrinops, agarwood cluster, microbe, inoculation
References:
[1] A. Hidayat, M. Turjaman, S. A. Faulina, F. Ridwan, Aryanto, Najmulah, T.T. Irawadi and Iswanto,
J. Korean Wood Sci. Technol. 47(4):459-471, (2019)
[2] A.A. Nasution, U.J. Siregar, Miftahudin and M. Turjaman, Journal of Forestry Research, (2019)
(Accepted 24 September 2018)
[3] M. Turjaman, Development of agarwood production cluster based on microbe technology,
translator: A. Susilo, IPB Press, 94 pages, (2019)
[4] S.Y. Lee, M. Turjaman and R. Mohamed, Tropical Life Sciences Research 29(2):13-28, (2018)

[5] A. Faizal, R.R. Esyanti, E.N. Aulinisa, Irawati, E. Santoso and M. Turjaman, Trees31:189-197,
(2017)
[6] M. Turjaman and A. Hidayat, Agarwood-planted tree inventory in Indonesia, in Proceeding of IOP
Conference Series: Earth and Environmental Science 54 (2017) 012062, Bogor, Indonesia, (2017)
[7] M. Turjaman and A. Hidayat, Valuation of derivatives non-timber forest products (NTFPs) of
agarwood cultivated based on endophytic microbial inoculation technology, in Proceeding of
IUFRO – INAFOR Joint International Conference 2017, pp. 131-138, Yogyakarta, Indonesia,
(2017)
[8] M.Turjaman, A. Hidayat and E. Santoso, Development of agarwood induction technology using
endophytic fungi inAgarwood: Science Behind the Fragrance,
[9] M. Rozi, Ed., Springer, pp. 57-72, (2016)

KS-07
Biodiversity, Biography and Conservation of Gyrinops Species
(Thymelaeaceae) in Indonesia
Tri Mulyaningsih1*), Isamu Yamada2 and Michiho Ito3
1
Faculty of Mathematics ang Natural sciences Universitas Mataram, Lombok, NTB, Indonesia.
2
Center for Southeast Asian Studies, Kyoto University, Japan
3
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Correspondence author email: trimulya@unram.ac.id
Abstract
The distribution of flora species is strongly influenced by geological history, such as the age / type
of rock type and also by the separation / fracture / disjunction of the earth's crust. This happens to
Gyrinops species (Thymelaeaceae) in Indonesia. There are 9 species of Gyrinops in the world that it was
known, but this number will continue to increase in line with intensive exploration. Two species
(Gyrinops walla and G. vadalii) from Lao, Sri Lanka and 7 species of Gyrinops come from Indonesia
that were distributed in the east of Wallace line. Every species has a specific geography range, such as
G. versteegii has geographical range in the Lesser Sunda Island area; G. decipiens in Celebes; G.
moluccana in Moluccas; G. podocarpus in the bird's head area of Papua grows in lime stone; G.
salicifolia is distributed in the part of northern Papua as long as Nabire to Abeparma; G. ladermannii
grew as long as longitudinal of the central of Guinea; and G. caudata spread in the part of Southern
Papua as long as Merauke to Timika Papua.
However, each island is composed by several rocks which have different ages, for example
Sumbawa island, the rocks were composed by recent volcanic formation (similar to Lombok) and
Cenozoic formation and there is Sumbawa strait between Lombok island and Sumbawa island, and
Sumba fault , which separate between Sumbawa Besar and Bima, so Gyrinops species that come from
tree places have differentiation morphology, stem anatomy and sitology, it can be able to promote to
create new species. Similar to Gyrinops species in Sulawesi, according to geological history Sulawesi
island was composed of 4 Sula's that collides, so there are many trusts, stripes, faults and many kinds of
rocks that arrange. Those conditions will create new species of Gyrinops. Such as Gyrinops decipiens
from central Sulawesi it grew on Ophicine, and thera are 4 other types that are found growing other
rock, such as the intrusive rock which was divided in two parts by Palu-Koro fault (namely Kambuno
Mt in South Luwuk and Quarles Hill in Mamasa), Paleogene volcanics in Gorontalo and Pliocene-
Recent Volcanic in Minahasa.
Conservation of Gyrinops species trees in Indonesia specifically does not yet exist but the
conservation of the habitat of other species such as the yellow-crested parrot in Aki Jawi, in Halmahera
and species of black Orchid in Kaimana Papua, which contains eaglewood trees habitat.
Keywords: Gyrinops, biodiversity, biography, conservation, Indonesia.
References:
[1] Idrus, A, S Prihatmoko, HG Hartono, F Idrus, Ernowo, Franklin, Moetamar and I Setiawan. 2014.
Some Key Features and Possible Origin of the Metamorphic Rock-Hosted Gold Mineralization
in uru Island, Indonesia. Indonesian Journal on Geoscience 1(1) 9-19.
[2] Hou, D. 1960. Thymelaeaceae. In: Van Steenis C.G.G.J. (ed.) Flora Malesiana. 6 (1): 1-
60.Wolters-Noordhoff Publishing. Groningen.

[3] Iswantari, W., T. Mulyaningsih., A. Muspiah. 2017. Karyomorfologi dan Jumlah Kromosom
Empat Grup Gyrinops versteegii (Gilg.) Domke. di Lombok. Jurnal Ilmu Kehutanan, (11): 205.
[4] Mulyaningsih, T. & I. Yamada. 2007. Notes on Some Species of Agarwood in Nusa Tenggara,
Celebes and West Papua. Faculty of Agriculture, Mataram University -Professor Emeritus, Kyoto
University.

ABSTRACT
ORAL PRESENTATION

SECONDARY USE, PHYTOCHEMISTRY,
TRADITIONAL MEDICAL,
PHARMACOLOGICAL ACTIVITY OF
AGARWOOD AND AROMATIC PLANTS

P-S1
The Effects of Aquilaria malaccensis Leaves Aqueous Extract on Sperm of
Sprague Dawley Rats towards Early Embryogenesis
Faridah Ismaila, Azantee Yazmie Abdul Wahabb, Muhammad Lokman Md Isac*, Hussin
Muhammadd, Raja Arif Shah Raja Ismailb, Redzuan Nul Hakim Abdul Razake, Muhammad Alif
Mazlanc
aDepartment of Basic Medical Sciences, Kulliyyah of Medicine, International Islamic University of Malaysia,
Kuantan Campus, Pahang, Malaysia
bDepartment of Obstetrics &Gynaecology, Kulliyyah of Medicine, International Islamic University of Malaysia,
cDepartment of Basic Medical Sciences, Kulliyyah of Nursing, International Islamic University of Malaysia,
dHerbal Medicine Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur,
Malaysia
eDepartment of Basic Medical Sciences, Kulliyyah of Allied Health Sciences, International Islamic University of
Malaysia, Kuantan Campus, Pahang, Malaysia
*Corresponding author’s email: lokman@iium.edu.my
Abstract
Oxidative stress induced by excessive and unopposed levels of reactive oxygen species in male
reproductive system results in impaired sperm quality, fertilization capacity and poor embryo
development. Our goal is to assess the potential effects of Aquilariamalaccensis (AM) leaves, a plant
with strong antioxidant property on early embryo development invitro and embryo quality following
fertilization with cyclophosphamide (CP) exposed rat sperm.
Twenty-four male Sprague Dawley rats were allocated into eight groups of three rats (n = 3):
control, CP only (200 mg/kg), AM only (100 mg/kg, 300 mg/kg and 500 mg/kg) and CP + AM (100
mg/kg, 300 mg/kg and 500 mg/kg). Animals were sacrificed after 63 days of treatment and sperm from
caudal epididymis were taken for invitro fertilization (IVF) with oocytes from untreated female.
Fertilization, embryo division and embryo morphology were examined at 8- and 48-hours post
insemination and compared between groups. Statistical evaluations were performed using Chi-Square
test and Fisher’s exact test and p-value<0.05 was considered significant.
Administration of AM leave extract at 100 mg/kg/day to normal rats and CP-exposed rats has
significantly increased (p<0.05) the fertilization rate, early cleavage rate and embryo quality when
compared to CP only treated group. However, other groups showed no significant differences.
Overall, the present results indicate the potential of AM leave extract supplementation to improve
the fertility and early embryo development in male rat exposed to CP by inhibiting the oxidative
processes and scavenging free radicals.
Keywords: Aquilaria malaccensis, cyclophosphamide, in vitro fertilization, embryo, natural
antioxidant.

OP-S2
A Review of Secondary Metabolites of Gyrinops versteegii (Gilg.) Domke for
Drugs and Cosmetic Material Purposes
Surya Hadia*), Baiq Desy Ratnasaria, Tri Mulyaningsiha, Kurniasih Sukentia, Yuliadi Zamronia, Ito
Michihob, Isamu Yamadac
aMathematics and Science Faculty, University of Mataram, West Nusa Tenggara, Mataram, 83121, Indonesia.
bPharmaceutical Science, Kyoto University, Kyoto, Japan.
cCenter for Southeast Asian Study Kyoto University, Kyoto, Japan.
*) Corresponding author email: sur_hadi88@yahoo.com
Abstract
Gyrinops versteegii (Gilg.) Domke is one of the agarwood producer which endemically grows in
Indonesia, specifically in Lesser Sunda, Sulawesi, and Papua. Unlike the renowned agarwood from the
species of genus Aquilaria, the G. versteegii is not yet familiar by many people in agarwood world. Few
researches showed that G. versteegii produces high quality resin with many potential secondary
metabolites. The metabolites in G. versteegii tend to be similar to the Aquilaria’s which has been
directed to use as drugs and cosmetic, which is focused as subject of this review.
Keywords: Agarwood, Gyrinopsversteegii, Aquilaria, drugs, cosmetic

OP-S3
Profiling of Compounds in Hydrosol Extract of Aquilaria (Agarwood)
Species using Gas Chromatography-Mass Spectrometry (GCMS)
Natasha Jafar Alia, Yumi Zuhanis Has-Yun Hashima*, Nur Aimi Aliah Zainurinb
bDepartment of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia
Corresponding author e-mail: yumi@iium.edu.my
Abstract
For many years, most researches involving agarwood mainly focused on extracting the agarwood
oil from the infiltrated resin, the most sought non-timber forest product from Aquilaria species which
has high demand and prices. This led to other parts of this valuable plant species being considered as
waste. Thisinclude the agarwood hydrosol (byproduct produced during the water distillation). Hydrosol
has been reported to retain certain aromaticsof its primary essential oil. Therefore, the present study is
conducted to identify the composition of the agarwood hydrosol extractsfromAquilaria species by using
gas chromatography-mass spectrometry (GCMS). From the analysis, there were a total of 46, 48, 40,
53, 58 and 62 compounds detected in hydrosol extract samples of 1, 2, 3, 4, 5 and 6 respectively.
Accordingly, the oxygenated and phenolic compounds were found to be present in all hydrosol extract
samples. The detected phenolic compounds include Phenol, 2,5-bis (1,1-dimethylethyl)-,.Phenol, 2,4-
bis (1,1-dimethylethyl)- and 9-Octadecenoic acid (Z)-, methyl ester. These compounds are believed to
possess various potential biological activities including antioxidant activities. Sample 1 and 2
consistsesquiterpene compounds such as (-)- Aristolene, Naphthalene, decahydro-4a-methyl-1-
methylene-7-(1-methylethylidene)-,(4aR- trans)-,Azulene,1,2,3,3a,4,5,6,7-octahydro-1,4-dimethyl-7-
(1-methylethenyl)-,[1R- (1.alpha.,3a.beta.,4.alpha.,7.beta.)]- andalloaromadendrene where the later has
the highest number of oxygenated and antioxidant compounds. To this end, it is noteworthy that findings
from this study have unravel the potential medicinal benefits of agarwood hydrosol and thus realizing
the waste to wealth paradigm.
Keywords: agarwood, Aquilaria, compounds, gas chromatography-mass spectrometry, hydrosol.
Table 1. Total number of compounds in each hydrosol extract samples of Aquilaria species identified
by gas chromatography-mass spectrometry analysis.
Total Total qualified Oxygenated Phenolic
Sample Sesquiterpene
compound peak (>80) compounds compounds
Blank 52 6 1 0 0
Sample 1 142 46 9 1 2
Sample 2 134 48 12 1 4
Sample 3 101 40 5 1 0
Sample 4 128 53 4 1 0
Sample 5 125 58 4 1 0
Sample 6 127 62 6 1 0

References:
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(Basel, Switzerland), 20(7), 11808–11829, (2015).
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[8] Y. Z. H-Y. Hashim, N.I. Ismail, and P. Abbas. IIUM Engineering Journal, 15(1), 55–60, (2014).

OP-S4
The Effect of Aquilaria malaccensis Leaves Aqueous Extract on Male
Reproductive System of Sprague Dawley Rats: Sperm Quality and
Testicular Histological Findings
Redzuan Nul Hakim Abdul Razaka,b, Muhammad Lokman Md Isab*, Hussin Muhammadc
a
Department of Biomedical Sciences, Kulliyyah of Allied Health Sciences, International Islamic University
Malaysia, Kuantan, Pahang, 25200, Malaysia.
b
Department of Basic Medical Science, Kulliyyah of Nursing, International Islamic University Malaysia,
Kuantan, Pahang, 25200, Malaysia.
c
Toxicology and Pharmacology Unit, Herbal Medicine Research Centre, Institute for Medical Research, Kuala
Lumpur, 50588, Malaysia.
Corresponding author E-mail: lokman@iium.edu.my
Abstract
Aquilaria malaccensis or locally known as ‘gaharu’ is a species of Aquilaria genus and belongs to
the Thymelaeaceae family. It is widely distributed in Malaysia, Indonesia, and the Borneo Islands.
Traditionally, its leaves were used to relieve bruises and studies have shown that they function as an
antioxidant, aphrodisiac, and tranquilizer. Despite its proven beneficial medicinal properties,
information regarding its toxicity is limited. Therefore, this study was conducted to investigate the
toxicity effect of A. malaccensis leaves aqueous extract (AM) on sperm quality and testicular histology.
The OECD Guideline 421 was selected to evaluate the male reproductive toxicity analysis which
comprised of control group (distilled water) and three AM-treated groups (100, 300 and 500mg/kg). 63
days of oral administration was carried out prior to the male necropsy. The epididymal sperm was
evaluated for motility, concentration, viability and morphology. The testicular section was processed
using routine histological process and evaluated semi-quantitatively using the Johnsen testicular biopsy
score followed by histomorphometric analysis.
There were no statistically significant (p>0.05) difference in the sperm concentration, motility,
viability and morphology of AM-treated groups compared to the control group. All AM-treated groups
indicated comparable mean Johnsen testicular biopsy score with the control group. The diameter of the
seminiferous tubules and the mean height of the germinal epithelium were not significantly different
(p>0.05) in comparison to control group. The data obtained revealed that AM did not exhibit toxicity on
male reproductive system and the no observed adverse effect level for male reproductive toxicity was
˃500mg/kg via oral route.
Keywords: Aquilaria malaccensis, male toxicity, sperm analysis, testicular histology.
References:
[1] Ajibade, T. O., & Olayemi, F. O., Andrologia, 47(9): 1034–1040 (2015).
[2] Cassuto, N. G., Hazout, A., Hammoud, I., Balet, R., Bouret, D., Barak, Y., Jellad, S., Plouchart, J.
M., Selva, J., & Yazbeck, C., Reproductive Bio Medicine Online, 24(2): 211–218 (2012).
[3] Chandel, M., & Jain, G. C., Journal of Biological Sciences and Medicine, 3(3): 1–14 (2017).
[4] Creasy, D M, & Chapin, R. E., Spermatogenesis, 4(2): e1005511 (2014).
[5] Hashim, Y. Z. H.-Y. Y., Kerr, P. G., Abbas, P., Mohd Salleh, H., & Salleh, H. M., Journal of

Ethnopharmacology, 189: 331–360 (2016).
[6] Organloktion for Economic Co-operation and Development. Test No 421: Reproduction /
developmental toxicity screening test. (2016).
[7] World Health Organization, WHO laboratory manual for the examination and processing of human
semen (2010).
[8] Santos, A. D. F. A., Vieira, A. L. S., Pic-Taylor, A., & Caldas, E. D., Brazilian Journal of
Pharmacognosy, 27(3): 353–360 (2017).
[9] Souza, A. C. F., Marchesi, S. C., Domingues de Almeida Lima, G., Ferraz, R. P., Santos, F. C., da
Matta, S. L. P., & Machado-Neves, M., Biological Trace Element Research, 171(2): 354–362
(2016).

OP-S5
GC-MS Analysis and Antibacterial Activity of Aquilaria malaccensis Leaf
Extract
Najihah Mohd Noora*, Yumi Zuhanis Has-Yun Hashima*, Ummu Athiyah Mohammad Tormizib,
Asmak Muti’ah Mohd Nasirc, Nur Umisha Zainudind
bBiotechnology programme, Institute of Biological Sciences, Faculty of Science, University Malaya,

cBiochemistry programme, Institute of Biological Sciences, Faculty of Science, University Malaya,

dDepartment of Biomedical Sciences, Kuliyyah of Allied Health Sciences, International Islamic University
Corresponding author e-mail: Najihahmnoor@gmail.com, yumi@iium.edu.my
Abstract. Ethnopharmacological practices have shown significant evidences of agarwood material

as having myriad of pharmacological activities including anticancer, antipyretic and antioxidant. The
complexity and variability of the chemical constitution of the plant establishes the quality of the
medicinal products. The present study was carried out to identify the phytochemical constituents of both
derivatized and non-derivatized Aquilaria malaccensisleaf ethanolic extract (ALEX) and to study the
anti-bacterial activity of ALEX extracted using Soxhlet extraction method. The metabolite profiling of
bioactive compounds of ALEX samples were conducted using gas chromatography - mass spectrometry
(GC-MS) coupled with the principal component analysis (PCA). The screening on the bacterial activity
of the extract was determined by using modified Kirby Bauer disc diffusion method against leading
bacterial pathogens: Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Vibrio
Vulnificus.GC-MS analysis of ALEX reveals a total of 127 compounds from the derivatized sample and
only 22 compounds in the non-derivatized sample. Several biological active compoundsthat werefound
in ALEX samples include hexadecanoic acid, trimethyl ester, hexadecenoic acid, ethyl ester, 2(4H)-
Benzofuranone, 5,6,7,7a-tetrahydro-4,4,7a- trimethyl-, trans-9-Octadecenoic acid, trimethylsilyl ester
and 4-Isopropylphenol, trimethylsilyl ether. ALEX showed comparable anti-bacterial activity with zone
of inhibition of 6.0 - 15.7 mmfor gram positive bacteria and 2.0 - 13.0 mm for gram negative bacteria
against control which is Dimethyl sulfoxide (DMSO). Penicillin was used as positive controlexhibited
strong zone of inhibition of 21.0 – 28.3 mm. The antibacterial activity results indicate that
ALEXpossessed mild to stronginhibitory activities against the pathogenic bacteria due to presence of
various phytochemical constituents which can be used as source of drugs to fight infections caused by
susceptible bacteria.
Keywords: GC-MS analysis, phytochemical constituents, antibacterial activity, Aquilaria malaccensis

Equation 1: Derivatization of hydroxylated polycyclic aromatic hydrocarbons into trimethylsilyl
(TMS) ethers using BSTFA.
References:
[1] Hashim, Y. Z. H.-Y., Ismail, N. I., & Abbas, P, IIUM Engineering Journal, 15(1), (2014).
[2] Lee, Y. C., Yunus, M. A. C., Idham, Z., Ruslan, M. S. H., Aziz, A. H. A. & Irwansyah, N.
Extractionand identification of bioactive compounds from agarwood leaves. IOP Conference
Series: Materials Science and Engineering, 102(2017), (2016).
[3] Ma, C. T., Eom, T., Cho, E., Wu, B., Kim, T. R., Oh, K. B., Han, S. B., Kwon, S. W. & Park, J.
H, Journal of Natural Products., 80, 3043–3048, (2017).
[4] Hendra, H., Moeljopawiro, S., &Nuringtyas, T. R., Antioxidant and antibacterial activities of
agarwood (Aquilaria malaccensis Lamk.) leaves. AIP Conference Proceedings 1755:1 140004,
(2016).
[5] Khalil, A. S., Rahim, A. A., Taha, K. K., & Abdallah, K. B., Journal of Applied and Industrial
Sciences,1(3), 78-88, (2013).

OP-S6
Agarwood Prescription in Malay Traditional Medical Manuscripts
Samsudin, Na*., Hashim, Y. Z, H-Ya., Mohammad Tormizi, U. A.b, Mohd Nasir, A. M.c, and
Zainudin, N. U.d
a
International Institute for Halal Research and Training, International Islamic University Malaysia, 53100
Gombak, Selangor
b
BiotechnologyProgramme, Institute of Biological Sciences, Faculty of Science, University Malaya,
c
BiochemistryProgramme, Institute of Biological Sciences, Faculty of Science, University Malaya,
d
Department of Biomedical Sciences, Kuliyyah of Allied Health Sciences, International Islamic University
*Corresponding author E-mail: nurhusna@iium.edu.my
Abstract
Aquilaria is a species of Agarwood which is well known by many names of Gaharu in Malay, Chen
xiang in China, Jin-ko in Japan and Oud in Arab countries. It is one of the most sought-after trees in the
world, for its many uses and valuable properties. Aside from the widespread use in the fragrance
industry, Agarwood has many applications in medicine. It is traditionally reported to be able to cure
many diseases such as joint-pain, diabetics and inflammatory-related ailment and diarrhoea. Thus, this
article is to discuss the applicability of Agarwood in Traditional Malay Medicine (TMM) documented
in old Malay text or manuscripts. The main source of manuscripts is from the collection of the Centre
of Malay Manuscript, National Library Malaysia. The scope of discussion comprises the prescriptions
of TMM that include Agarwood as one of the elements of the remedies, types of diseases, process of
preparing the prescription, method of treatment as well as taboos. Findings from this work is expected
to be able to verify the traditional use of Agarwood towards the discovery of new evident-based
alternative medicine while safeguarding TMM as national heritage.
Keywords: Agarwood, Medical manuscript, Gaharu, TMM.
Figure 1. Example of (a) original Malay medical manuscript (Kitab Tib at ISTAC, IIUM), (b)
transliterated manuscript and excerpt (Kitab Muzium Terengganu; Harun Mat Piah, 2017).

References:
[1] Hussain, A. G. (2015). MSS 2999 Kitab Tib: Pandangan dan Tafsiran Perubatan Moden Terhadap
Manuskrip Perubatan Melayu. Kuala Lumpur: Institut Penyelidikan Perhutanan Malaysia 2015.
[2] Mustapha, N. M., Nik Mahmood, N. Z. and Mohd Ali, N. A. (2017). Khazanah Perubatan Melayu
Tumbuhan Ubatan Jilid 1. Kuala Lumpur: FRIM.
[3] Piah, H. (2006). Kitab Tib Ilmu Perubatan Melayu. Kuala Lumpur: Institut Penyelidikan
Perhutanan Malaysia 2015.
[4] Abdu’allah, (2017). Kitab Perubatan Melayu Khazinat Al- Insan Perbendaharaan Manusia. Kuala
Lumpur: Akademi Jawi Malaysia - Klasika Media.
[5] Piah, H. (2017). Kitab Tib Muzium Terengganu. Kuala Lumpur: FRIM.
[6] Piah, H. and Baba, Z. (2014). Kitab Tib MSS 2515. Kuala Lumpur: Penerbit UKM
[7] KutaKarang, S. A. (2017). Kitab Perubatan Melayu Al-Rahmah Fi Al-TibbWa Al- Hikmah. Kuala
Lumpur: Akademi Jawi Malaysia - Klasika Media.

INDUCTION OF AGARWOOD, CEMMERCE,
CHEMICAL CONSTITUENTS
OF AGARWOOD & AROMATIC PLANTS

OP-I1
The Best Inoculation Technique Applied on Gyrinopsversteegii Tree Trunk
at Height of 4 – 5 meters
Resti Wahyunia*, Amalia Indah Prihantinia
aNon Timber Forest Product Technology Research and Development Institute, Jalan Dharma Bhakti No.7, Ds
Langko, Lingsar, Lombok Barat, NTB
Corresponding author email: resti_bio@yahoo.com
Abstract
Agarwood is a non-timber forest product having high economic value; however, its population
in nature is getting decrease. Gyrinopsversteegii, an agarwood- producing plant, has been listed in
Apendix II CITES; therefore, hunting the agarwood in nature must be reduced. An effort to reduce the
agarwood hunting in the nature is agarwood cultivation. Agarwood cultivation includes cultivation of
agarwood-producing plant and agarwood inoculation. Some of agarwood inoculation methods are
simpori and implant technique.
Simpori is a modified inoculation method using porous nail and Fusarium solani, meanwhile
implant technique use drill and piece of wood that is soaked in F. solani spores. The present study aimed
to determine the best inoculation technique applied to G. versteegii tree trunk at height of 4 – 5 meter.
This study using completely randomizes design with 2 treatments (simpori and implant technique) and
9 replications.
The result showed that the agarwood produced at 6 months after inoculation was not significant
in all treatment. The rendemen average of agarwood production produced by simpori and implant
technique are 0.66 and 0.64 % respectively. Based on SNI 7631:2011, the visual quality of agarwood
equaled to kemedangan TG.C. In conclusion, Simpori and implant technique produce agarwood in
similar quality and quantity at 6 months inoculation, however simpori more practical for application in
the field.
Keywords: agarwood, implant, simpori
Figure 1. Agarwood produced by simpori and implant technique at 6 months inoculation.
References :
[1] CITES. Appendix II of convention on international trade in endangered species of wild fauna and
flora. Accesed from https://www.cites.org/eng/app/appendices.php (2017, Oktober).
[2] Kakino M, Tazawa S, Maruyama H, Tsuruma K, Araki Y, Shimazawa M, Hara H. Laxative effects
of agarwood on low-fiber diet-induced constipation in rats. BMC Complementary and Alternative

Medicine. 10:68-75, (2010).
[3] Anggadhania L, Nugraheni YMM, Suryawan IG, Soetarto ES. Peningkatan strain
kapangpembentukgaharu pada jenisGyrinopsversteegiidi NTB. Laporan Hasil Penelitian Balai
PenelitianTeknologi HHBK. Mataram, (2014).
[4] [BSN] Badan Standardisasi Nasional. SNI Gaharu 7631-2011, (2011).
[5] Sasmuko S.A. Uji Coba Teknik InokulasiGaharu di NTB. Laporan Hasil Penelitian Balai Penelitian
Teknologi HHBK. Mataram, (2011).
[6] Sasmuko S.A., Nugraheni YMM, Setyayudi A, Mansyur. Eksplorasi dan Isolasi Cendawan
Pembentuk Gaharu di NTB. Laporan Hasil Penelitian Balai Penelitian Teknologi
HHBK. Mataram, (2011).
[7] Sasmuko S.A., Kurnaidi. Teknik Inokulasi Gaharu dengan Simpori di NTB. Laporan Hasil
Penelitian Balai Penelitian Teknologi HHBK. Mataram, (2013).

OP-I2
Bioinduction of Agarwood in Cultivated Gyrinops verstegii (Gilg.) Domke
using Fusarium solani
Ahmad Faizala*, Alda Wydia Prihartini Azara, and Maman Turjamanb
aPlant Science and Biotechnology Research Group, School of Life Sciences and Technology,
Institut Teknologi Bandung, Ganesa 10 Bandung 40132, Indonesia

bForest Microbiology Research Group, R&D Centre for Forest, Environment & Forestry Research,
Development, and Innovation Agency (FORDA), Ministry of Environment and Forestry,
Jl. Gunung Batu 5, Bogor, 16680, Indonesia
Corresponding author e-mail: afaizal@sith.itb.ac.id
Abstract
Agarwood is a resinous product containing secondary metabolites produced by Thymelaeaceae,
which requires a very long accumulation in nature. Therefore, various induction techniques have been
explored to artificially induce agarwood in cultivated plants and to shorten its process. In this study, one
of the most abundant agarwood-producing trees in Indonesia, namely Gyrinopsversteegii was injected
and inoculated with two different strains of endophytic fungi Fusariumsolani isolated from Gorontalo
and Jambi Provinces. The results revealed that the inoculated G. verstegii generates extensive resinous
zone on the wood tissues. Gas chromatography (GC-MS) analysis of the fungal treated samples allowed
the detection of several agarwood markers such as Alloaromadendrene, beta-Eudesmol and beta-
Selinene of sesquiterpenes and chromones derivatives including 2-(2-phenylethyl) chromen-4-one, 6-
methoxy-2-(2-phenylethyl) chromen-4-one dan 6,7-dimethoxy-2-(2- phenylethyl) chromen-4-one.
Taken together, this technique would be beneficial not only to develop the induction of artificial
agarwood, but also to stimulate it rapid accumulation in cultivated agarwood producing trees.
Keywords: Artificial agarwood, bioinduction, chromones, endophytic fungi, sesquiterpenes
Healthy plant GSL1 GSL2 Wounded (PDB)
Figure 1. Resinous zone developed around wounding site on G. versteegii with or without inoculation
of different strain of F. solani (GSL1 and GSL2) (pictures were taken 4 weeks after inoculation)

Figure 2. Olfactory test of powdered and burned agarwood samples from G. versetegii. 1 Nearly
odorless, 2 fair odor, 3 less strong odor, 4 strong odor, 5 very strong and pleasant odor.
References:
[1] A. Faizal, R. R. Esyanti, E. N. Aulianisa, Iriawati, E. Santoso, and M. Turjaman, Trees 31: 189-
197, (2017)
[2] R. Kalra, and N. Kaushik, Phytochemistry Rev,16: 1045-1079, (2017)
[3] A. A. Nasution, U. J Siregar, and M. Turjaman, J Forest Res,(2019) (in print)
[4] C. S. Tan, N. M. Isa, I. Ismail, and Z. Zainal, Front Plant Sci, 10: 1-13

OP-I3
The Effectiveness of Gaharu Induction by Fungal Inoculation on Gyrinops
versteegii Gilg. Domke
Lutfi Anggadhania L.1, YMM Anita Nugraheni2 and Nurul Wahyuni1*
1
NTFP Technology Research and Development Institute
2
Research and Development Center for Forest Plant Seed Technology
*) nunik.colin@gmail.com
Abstract
The high value of agarwood causes an increase of agarwood trade. The forming of agarwood in
natural life will take a long time so it is not able to fulfill market demand. Many cultivation and
inoculation techniques of agarwood were developing. This study was determining the effectiveness of
agarwood induction by the inoculation origin fungi from West Nusa Tenggara on Gyrinops versteegii
in West Lombok. The results showed that after 6 months ofinoculation means of the areawith
highinfectionseen infungalLT isolateswith a value of2728 mm2, whileM, ALS, and thecontrol isolates,
respectively for 543mm2, 281.3mm2, and 166mm2. The fragrant andcolorparametersof each
isolateshowed nosignificant differences. LT fungal isolates have 73% effectiveness, fungal isolates of
ALS and M have 7 and 14% effectiveness. Effectiveness significantly (p ≤0.05) is shown in the area
parameter.
Keywords: agarwood, effectiveness ofinduction, fungi
Figure 1.The formation of ALS (A), LT (B), M (C) and Control (D) area, (1, 3 and 6 month)

Figure 2. Agarwood development based on area of three isolates of fungi forming agarwood origin
NTB in West Lombok.
References:
[1] Akter, S., Islam, M. T., Zulkefeli, M., & Khan, S. I. International Journal of Pharmaceutical and
Life Sciences, 2(1), 22–32, (2013)
[2] Chen, H., Yang, Y., Xue, J., Wei, J., Zhang, Z., & Chen, H.. Molecules, 16(6), 4884–4896, (2011)
[3] CITES. Appendices I, II, and III of CITES. Bangkok. Thailand.: CITES. Retrieved from
https://www.cites.org/eng/app/appendices.php. (2017).
[4] Hashim, Y. Z. H. Y., Kerr, P. G., Abbas, P., & Mohd Salleh, H. Journal of Ethnopharmacology,
189, 331–360, (2016)
[5] Huang, J. Q., Liao, Y. C., Chen, H. J., & Zhang, Z. Phytochemistry Letters, 19, 64–70, (2017)
[6] Persoon, G. A. (2008). Smallholder Tree Growing for Rural Development and Environmental
Services. (R. D. Snelder, D.J. and Lasco, Ed.). Springer.(2008)
[7] Lee, SY., Emang, D., Rossi M. Journal of Forestry Research, (March), (2018)

OP-I4
History of the Agarwood of the Arabs
Hussain Ahmed AL Rifaei
Doctor retired, previously worked in the Department of Education and
Knowledge In U.A.E Correspondence author email:
dr.hussain_uae@hotmail.com
Abstract
The agarwood has been known to Arabs since very long time.
Hundreds of years before the Prophet Muhammad's mission, the Arabs
used the agarwood. Arabs use agarwood in their joys and sorrows and in
religious and social occasions. agarwood was and still is a manifestation
of their daily lives. Agarwood has more than forty names among Arabs,
this is a great indication of how interested they are.The ancient Arabs set
accurate standards for the quality of the agarwood.In presentation using
the power point, more details will be unveiling.
The author has been granted the Ph.D degree from Mohammed
V University In the year 2012 . The subject of the Ph.D was on The
relationship between Arabs and the agarwood.The author has visit almost
all the jungles where the agarwood grow in India, Sri Lanka, Indonesia,
Vietnam, Papua New Guinea, Myanmar, Cambodia Hence rich
information
has bebuilt up.
The author has met so many scientists around the world and
Agarwood distillers in Laos, Malaysia, Indonesia Thailand, India,
Bangladesh.
Key words: History, agarwood, Arab.
References:
Notes: Sources and references are Arabic books

‫‪ -1‬اآلداب الشرعية والمنح المرعية‪ ،‬اإلمام أبي عبد هلال محمدد بدم مح دح الميد دي‪ ،‬قحييديب ادريؤ ا‬ ‫ا‪/‬ق فتحي السديد‪،‬‬
‫دا‪ /‬الكتؤ الر مية – بيروت‪2006 ،‬م‪ / ،‬دمو ل عمدر الييدام‪،‬‬
‫مم سة الر الة ‪ -‬بيروت‪1417 ،‬هـ ‪1996 -‬م ‪. 2 . ،‬‬ ‫‪.1.‬‬
‫‪ -2‬أدب الكاقؤ‪ ،‬أبو محمد عبد هلال بم مس م بم قتيبة السكوفي المروزي الدينو‪/‬ي‪ ،‬قحييي ب محمدد محدي‬
‫الدديم عبدد الحميدد‪،‬‬
‫‪ /‬في غرائؤ ا مصا‪ /‬وعجائؤ ا حا‪ ،)/‬مكتبة السرادة ‪ -‬مصر‪1963 ،‬م ‪. 4 . ،‬‬
‫محمد بم عبدهلال بم محمد ال واقي أبو ‪ -3‬ا ز‪/‬ق‪ ،‬محمد بم اصر بم مان‪ ،‬وزا‪/‬ة التراث اليومي والثيافة – عمان‪1402 ،‬هـ ‪1982 -‬م‬
‫‪.‬‬ ‫عبدهلال‪،‬‬
‫قحيييب د‪ .‬ع ي المنتصر الكتا ي‪ ،‬مم سة ‪ -4‬أ اس البالغة‪ ،‬أبو اليا م محمود بم عمر بم محمد بم عمر الخوا‪/‬زمي الزمخشري‪ ،‬دا‪ /‬الحكر‪،‬‬
‫الر الة – بيروت‪1405 ،‬هـ‪1399 .-23 4 . ،‬هـ ‪1979 -‬م‪ -5 .‬اال تيصا خبا‪ /‬دول المغرب ا قصى‪ ،‬أبو الرباس أحمد بم خالد بم محمد‬
‫الناصري‪ ،‬قحيييب جرحر الناصري و محمد‬ ‫‪/‬ح ة ابم بطو ة (قححة الن‬
‫الناصري‪ ،‬دا‪ /‬الكتاب ‪ -‬الدا‪ /‬البيضاء‪1418 ،‬هـ ‪1997 -‬م ‪.‬‬
‫‪-6‬أ س قا‪/‬يخ اإل الم‪ ،‬د‪ .‬حسيم مم س‪ ،‬الزهراء لإلعالم الرربي – الياهرة‪.1 . ،‬‬
‫‪ -7‬ا غا ي‪ ،‬أبو الحرج ا صبها ي‪ ،‬قحيييب ع ي مهنا و مير جابر‪ ،‬دا‪ /‬الحكر – لبنان‪.‬‬
‫‪ -8‬ا ساب ‪ ،‬أبو ريد عبد الكريم بم محمد بم منصدو‪ /‬التميمدي السدمرا ي‪ ،‬قحييديب عبدد هلال عمدر‬
‫البدا‪/‬ودي‪ ،‬دا‪ /‬الحكدر –‬
‫بيروت‪1998 ،‬م ‪. 1 . ،‬‬
‫‪ -9‬ا س الج يل بتا‪/‬يخ اليدس والخ يل‪ ،‬مجير الديم الحنب ي الر يمي‪ ،‬قحيييب عد ان يو س عبد المجيد‬
‫باقدة‪ ،‬مكتبدة د دديس‬
‫‪ -‬عمان‪1420 ،‬هـ ‪1999 -‬م ‪.‬‬
‫‪ -10‬ا وائل‪ ،‬أبو هالل الحسم بم عبدهلال بم هل الرسكري‪ ،‬وضع حواايهب عبدالرزاق غالؤ‬
‫المهددي‪ ،‬دا‪ /‬الكتدؤ الر ميدة‬
‫– بيروت‪1417،‬هـ ‪1997-‬م‪.1 . ،‬‬
‫‪ -11‬البداية والنهاية‪ ،‬إ ماعيل بم عمر بم كثير اليراي أبو الحداء‪ ،‬مكتبة المرا‪/‬ف – بيروت ‪.‬‬
‫‪ -12‬قاج الرروس مم جواهر الياموس‪ ،‬محمد مرقضى الحسيني الزبيدي‪ ،‬قحييي ب مجموعة مم‬
‫المحيييم‪ ،‬دا‪ /‬الهداية‪ -13 .‬قا‪/‬يخ اإل الم ووفيات المشاهير وا عالم‪ ،‬امس الديم محمد بم أحمد بم‬
‫عثمان الذهبي‪ ،‬قحييديب د‪ .‬عمدر عبدد السدالم‬
‫قدمري‪ ،‬دا‪ /‬الكتاب الرربي ‪ -‬بيروت ‪1407 -‬هـ ‪1987 -‬م ‪. 1 . ،‬‬
‫‪ -14‬قا‪/‬يخ مديندة دمشدي وركدر فضد ها وقسدمية مدم ح هدا مدم ا ماادل‪ ،‬أبدو اليا دم ع دي بدم الحسدم‬
‫بدم هبدة هلال بدم عبدد هلال‬
‫الشافري‪ ،‬قحيييب محؤ الديم أبي ريد عمر بم غرامة الرمري‪ ،‬دا‪ /‬الحكر ‪ -‬بيروت‪1995 ،‬م ‪.‬‬
‫‪ -15‬التجا‪/‬ة والمالحدة فدي الخ ديال خدالل اليدرن السدابع عشدر ‪ ،‬د ‪ .‬عبددالرزاق محمدود المردا ي ‪ ،‬دائدرة‬
‫الثيافدة واإلعدالم –‬
‫الشا‪/‬قة‪2001 ،‬م ‪. 1 . ،‬‬
‫‪ -16‬اما‪ /‬الي وب فدي المضداف والمنسدوب‪ ،‬أبدو منصدو‪ /‬عبدد الم دم بدم محمدد بدم إ دماعيل‬
‫الثردالبي النيسدابو‪/‬ي‪ ،‬قحييديب‬
‫محمد أبو الحضل إبراهيم‪ ،‬دا‪ /‬المرا‪/‬ف – الياهرة ‪.‬‬
‫‪ -17‬الج يس الصالح الكافي وا يس الناصح الكافي‪ ،‬أبو فرج المرافى بم زكريدا النهروا دي الجريدري‪،‬‬
‫د‪/‬ا دة وقحييديب د‪.‬‬
‫محمد مر ي الخولي‪ ،‬مطبرة عالم الكتؤ ‪ -‬بيروت‪1423 ،‬هـ ‪1993 -‬م‪.1. ،‬‬
‫‪ -18‬جمهرة ال غة‪ ،‬ابم د‪/‬يد ا زدي‪ ،‬قحيييب ‪/‬مزي منير بر بكي‪ ،‬دا‪ /‬الر م ل مالييم – بيروت‪،‬‬
‫‪1987‬م‪.1 . ،‬‬
‫‪ -19‬خزا ة ا دب ولؤ لباب لسان الررب‪ ،‬عبد الياد‪ /‬بم عمر البغدادي‪ ،‬قحيييب محمد بيدل ريحدي‬
‫وأميدل بدديع اليريدوب‪،‬‬
‫دا‪ /‬الكتؤ الر مية ‪ -‬بيروت‪1998 ،‬م‪. 1 . .‬‬
‫‪ -20‬الد‪ //‬الكامنة في أعيدان المائدة الثامندة‪ ،‬الحدافا ادهاب الدديم أبدو الحضدل أحمدد بدم ع دي بدم‬
‫محمدد الرسديال ي‪ ،‬قحييدي‬
‫ومراقبةب محمد عبد المريد ضان‪ ،‬مج س دائرة المرا‪/‬ف الرثما ية ‪ -‬حيد‪ /‬آباد‪ -‬الهند‪1392 ،‬هـ ‪-‬‬
‫‪1972‬م ‪.2 . ،‬‬
‫‪ -21‬الذخيرة في محا م أهل الجزيرة‪ ،‬أبو الحسم ع ي بم بسدام الشدنتريني‪ ،‬قحييديب إحسدان عبداس‪،‬‬
‫دا‪ /‬الثيافدة ‪ -‬بيدروت‪،‬‬
‫‪1417‬هـ ‪1997 -‬م‪.‬‬
‫‪/ -22‬بيع ا برا‪ /‬وفصوص ا خبا‪ /‬في المحاضرات‪ ،‬أبو اليا م محمود بم عمر الزمخشري‪ ،‬قحيييب‬
‫‪1st International Symposium on Agarwood and Aromatic Plants 2019‬‬

‫‪Golden Palace Hotel Mataram, October 1-2, 2019‬‬ ‫‪60‬‬
‫‪ -24‬زاد المراد في هدي خير الرباد‪ ،‬محمد بم أبي بكر أيوب الز‪/‬عي أبو عبد هلال ‪ ،‬قحيييب ادريؤ ا ‪ /‬داطو وعبدد اليداد‪/‬‬
‫ا ‪ /‬اطو ‪ ،‬مم سة الر الة ‪ -‬مكتبة المنا‪ /‬اإل المية ‪ -‬بيروت ‪ -‬الكويت‪1407 ،‬هـ ‪1986 -‬م‪. 14 . ،‬‬
‫‪ -25‬يط الز د ‪ ،‬أبو الرالء المرري ‪ ،‬دا‪ /‬صاد‪ /‬ـ بيروت ‪.‬‬
‫‪ -26‬السيرة الح بية في يرة ا ميم المأمون‪ ،‬ع ي بم برهان الديم الح بي‪ ،‬دا‪ /‬المررفة ‪ -‬بيروت‪1400 ،‬هـ ‪.‬‬
‫‪ -27‬صبح ا عشى في صناعة اإل شا‪ ،‬الي يشندي أحمد بم ع ي بم أحمد الحزا‪/‬ي‪ ،‬قحيييب عبد الياد‪ /‬زكا‪ ،/‬وزا‪/‬ة الثيافة ‪-‬‬
‫دمشي‪1981 ،‬م ‪.‬‬
‫‪ -28‬صحيح البخا‪/‬ي (الجامع الصحيح المختصر)‪ ،‬محمد بدم إ دماعيل أبدو عبددهلال البخدا‪/‬ي الجرحدي‪ ،‬قحييديب د‪ .‬مصدطحى‬
‫ديؤ البغا‪ ،‬دا‪ /‬ابم كثير‪ ،‬اليمامة ‪ -‬بيروت ‪. 3 . ،1987 - 1407 -‬‬
‫‪ -29‬صحيح مس م‪ ،‬مس م بم الحجاج أبو الحسيم اليشديري النيسدابو‪/‬ي‪ ،‬قحييديب محمدد فدماد عبدد البداقي‪ ،‬دا‪ /‬إحيداء التدراث‬
‫الرربي – بيروت‪.‬‬
‫‪ -30‬صحة جزيرة ا دلس منتخبة مم كتاب الروض المرطا‪ /‬في خبر ا قطا‪ ،/‬أبو عبد هلال محمد بم عبد هلال بم عبد المدنرم‬
‫الحميري‪ ،‬قحيييب إ ‪ .‬الفي بروفنصال‪ ،‬دا‪ /‬الجيل ‪ -‬بيروت‪ 1408 ،‬هـ ‪1988 -‬م‪. 2 . ،‬‬
‫‪ -31‬عيون ا باء في بيدات ا بداء‪ ،‬موفدي الدديم أبدو الربداس أحمدد بدم اليا دم بدم خ يحدة بدم يدو س السدردي الخز‪/‬جدي‪،‬‬
‫قحييي ب د‪ .‬زا‪/ /‬ضا‪ ،‬دا‪ /‬مكتبة الحياة ‪ -‬بيروت ‪.‬‬
‫‪ -32‬الرالقات بيم الدولدة الربا دية والصديم فدي الرصدريم الربا دييم ا ول والثدا ي‪ ،‬د‪ .‬يو د صدير‪ ،‬المكتبدة الرصدرية –‬
‫بيروت ‪ 1432 ،‬هــ ‪ 2011 -‬م‪. 1 . ،‬‬
‫‪ -33‬فاكهة ابم السبيل ‪/ ،‬ااد بم عمير بم اا ي يم خ بم هاادم ‪ ،‬وزا‪/‬ة التدراث اليدومي والثيافدة – عمدان ‪1405 ،‬هدـ ‪-‬‬
‫‪1984‬م ‪.‬‬
‫‪ -34‬اليا ون في الطؤ‪ ،‬ابم ينا‪ ،‬ارح وقرقيؤب جبران ج بو‪ ،/‬مكتبة المرا‪/‬ف – بيروت‪1984 ،‬م‪. 4 . ،‬‬
‫‪ -35‬كنز الرمال في نم ا قوال وا فرال‪،‬عالء الديم ع دي المتيدي بدم حسدام الدديم الهنددي‪ ،‬دا‪ /‬الكتدؤ الر ميدة – بيدروت‪،‬‬
‫‪1419‬هـ ‪. 1 . ،1998 -‬‬
‫‪ -36‬لسان الررب‪ ،‬محمد بم مكرم بم منظو‪ /‬ا فرييي المصري‪ ،‬دا‪ /‬صاد‪ -/‬بيروت ‪. 1 . ،‬‬
‫‪ -37‬المحؤ والمحبوب والمشموم والمشروب‪ ،‬السري بم أحمد الرفاء ‪ ،‬قحيييب مصباح غالو جي‪ ،‬مطبوعات مجمدع ال غدة‬
‫الرربية ــ دمشي ‪.‬‬
‫‪ -38‬والمحيط ا عظم‪ ،‬أبو الحسم ع ي بدم إ دماعيل بدم ديدم المر دي‪ ،‬قحييديب عبدد الحميدد هندداوي‪ ،‬دا‪ /‬الكتدؤ الر ميدة‪-‬‬
‫بيروت‪2000 ،‬م‪.1 . ،‬‬
‫‪ -39‬المخصص‪ ،‬أبو الحسم ع دي بدم إ دماعيل النحدوي ال غدوي ا دلسدي’ قحييديب خ يدل إبدراهم جحدال‪ ،‬دا‪ /‬إحيداء التدراث‬
‫الرربي – بيروت‪1417 ،‬هـ ‪1996 -‬م‪. 1 . ،‬‬
‫‪ -40‬المستطرف في كل فم مستظرف‪ ،‬اهاب الدديم محمدد بدم أحمدد أبدي الحدتح ا بشديهي‪ ،‬قحييديب محيدد محمدد قميحدة‪ ،‬دا‪/‬‬
‫الكتؤ الر مية – بيروت‪1406 ،‬هـ ‪1986‬م ‪. 2 . ،‬‬
‫‪ -41‬المرا‪/‬ف‪ ،‬ابم قتيبة أبو محمد عبد هلال بم مس م ‪ ،‬قحيييب د‪ .‬اروت عكااة‪ ،‬دا‪ /‬المرا‪/‬ف ‪ -‬الياهرة‪.‬‬
‫‪ -42‬مرجم ا لحاظ الرامية في دولدة اإلمدا‪/‬ات الرربيدة المتحددة‪ ،‬د‪ .‬فدالح حنظدل‪ ،‬قيدديم ومراجردةب د‪ .‬غسدان الحسدم‪ ،‬اإلدا‪/‬ة‬
‫الثيافية بوزا‪/‬ة اإلعالم والثيافة – دولة اإلما‪/‬ات الرربية المتحدة‪1998 ،‬م‪. 2 . ،‬‬
‫‪ -43‬مرجم الب دان‪ ،‬ياقوت بم عبد هلال الحموي أبو عبد هلال‪ ,‬دا‪ /‬الحكر – بيروت‪.‬‬

‫‪ -44‬مرجم ما ا ترجم مم أ ماء البالد والمواضدع‪ ،‬عبدد هلال بدم عبدد الرزيدز البكدري ا دلسدي أبدو عبيدد‪ ،‬قحييديب مصدطحى‬
‫السيا‪ ،‬عالم الكتؤ ‪ -‬بيروت ‪1403 -‬هـ ‪. 3 . ،‬‬
‫‪ -45‬مرجم مياييس ال غة‪ ،‬أبو الحسيم أحمد بم فا‪/‬س بم زكريا‪ ،‬قحييديب عبدد السدالم محمدد هدا‪/‬ون‪ ،‬دا‪ /‬الجيدل – بيدروت‪،‬‬
‫‪1420‬هـ ‪1999 -‬م‪. 2 ،‬‬
‫‪ -46‬ميدمة ابم خ دون‪ ،‬عبد الرحمم بم محمد بم خ دون الحضرمي ‪ ،‬دا‪ /‬الي م – بيروت‪1984 ،‬م‪. 5 . ،‬‬
‫‪ -47‬مم كنوز الطؤ الرربي‪ ،‬أبو الحداء محمد عزت محمد عا‪/‬ف‪ ،‬دا‪ /‬الحنون ل طباعة والنشر والتغ ي – جدة ‪ -48 .2. ،‬النجوم الزاهرة‬
‫في م وك مصر والياهرة‪ ،‬جمال الدديم أبدو المحا دم يو د بدم قغدري بدردك ا قدابكي‪ ،‬وزا‪/‬ة الثيافدة‬
‫واإل‪/‬ااد اليومي – مصر ‪.‬‬
‫‪ -49‬زهة المشتاق في اختراق اآلفاق‪ ،‬أبو عبد هلال محمد بم عبد هلال بم إد‪/‬يس الحمدودي الحسدني‪ ،‬عدالم الكتدؤ – بيدروت‪1409 ،‬هددـ ‪1989 -‬م‬
‫‪ -50. 1 . ،‬شددوا‪ /‬المحاضددرة وأخبددا‪ /‬المددذاكرة‪ ،‬أبددو ع ددي المحس دم ب دم ع ددي ب دم محمددد التن دوخي‬
‫البصري‪ ،‬قحيييب مصطحى حسيم عبد الهادي‪ ،‬دا‪ /‬الكتؤ الر مية ‪ -‬بيروت‪1424 ،‬هـ ‪2004 -‬م ‪. 1 . ،‬‬
‫‪ -51‬حح الطيؤ مم غصم ا دلس الر يدؤ‪ ،‬أحمدد بدم محمدد الميدري الت مسدا ي‪ ،‬قحييديب د‪ .‬إحسدان عبداس‪ ،‬دا‪ /‬صداد‪– /‬‬
‫بيروت‪1388 ،‬هـ ‪.‬‬
‫‪ -52‬هاية ا ‪/‬ب في فندون ا دب‪ ،‬ادهاب الدديم أحمدد بدم عبددالوهاب الندويري‪ ،‬قحييديب محيدد قمحيدة وجماعدة‪ ،‬دا‪ /‬الكتدؤ‬
‫الر مية – بيروت‪1424 ،‬هـ ‪2004 -‬م‪. 1 . ،‬‬
‫‪ -53‬هدية الرا‪/‬فيم أ دماء المدملحيم وآادا‪ /‬المصدنحيم‪ ،‬إ دماعيل باادا البغددادي‪ ،‬دا‪ /‬الكتدؤ الر ميدة – بيدروت‪1413 ،‬هدـ –‬
‫‪1992‬م ‪.‬‬
‫‪ -54‬الوافي بالوفيات‪ ،‬صالح الديم خ يل بم أيبم الصحدي‪ ،‬قحيييب أحمد ا ‪ /‬اطو و قركي مصدطحى‪ ،‬دا‪ /‬إحيداء التدراث‬
‫– بيروت‪1420 ،‬هـ ‪2000 -‬م ‪.‬‬

OP-I5
Eaglewood Cultivation and Marketing in West Nusa Tenggara
Sudirman
CV. Griya Agroforestry, Kekait, Gunung Sari District, West Lombok, Indonesia
Abstract
Eaglewood cultivation in West Nusa Tenggara Barat has been carried out from 1995 until now, both
individually and through government programs. The involvement of farmers in developing agarwood in
NTB is quite large. This cultivation activity is carried out on privately owned gardens of community
forest lands and yards. This has been done directly involved in cultivation activities carried out in
accordance with ability. The problem currently faced is that agarwood cultivation can improve the
welfare of farmers. Farmers' efforts and strategies to increase income from agarwood cultivation. 1.
Doing nurseries 2. Planting agarwood to replace plants that have been harvested. 3. Perform inoculation
with nails & microbes.4. Selling seeds and seeds.
The current marketing of agarwood by farmers is still difficult because the issue of the quality of
agarwood, pig or oil products is only bought by local entrepreneurs so that they are not in favor of
farmers. Sale of aloes agarwood is currently in reduced demand from outside the region.
The hope of farmers to all stakeholders’ aloes lovers to help farmers process agarwood into high
quality products and can be marketed by farmers and specifically the government to provide agarwood
distillation tools for farmers.
Keywords: eaglewood seedling, cultivation, agarwood marketing, West Nusa Tenggara Barat.

OP-I6
Morphological and Gowth Characteristics of Endophytic Phomopsis sp. and
Aspergillus sp. from Gyrinops verteegii
Ernin Hidayatia*, Tri Mulyaningsiha, Faturrahmana, Sarkonoa, Nilam Fadmaulidha Wulandarib, Ito
Michihoc, Isamu Yamadad
aDepartment of Biology, Faculty of Mathematics and Natural Sciences, University of Mataram, Indonesia.
bIndonesian Institute of Science, Cibinong, Indonesia
c Faculty of Pharmaceutical Science, Kyoto University, Japan
d Center for Southeast Asian Studies, Kyoto University, Japan
Corresponding author E-mail: hidayatiernin@unram.ac.id
Abstract
In association with plants, fungi can act as plant endophytes, pathogens, and saprobes. Endophytic
fungi are a group of fungi that live in their host tissues without causing diseases. Its presence in the host
tissues can be beneficial to the host. Endophytic fungi in Gyrinops is still needs to be explored while the
existence of this plant in nature continue to decrease.
The aims of this study was to characterise the morphology and growth of Phomopsis sp. and
Aspergillus sp., two endophytic fungi isolated from Gyrinops versteegii. The plant samples usedin this
studywere collected from Pusuk Forest, West Lombok, Indonesia. The cutting parts of plant
wereinoculated on Potato Dextrose Agarplate.Twenty isolates obtained from Gyrinops versteegii. Two
of the isolate are Phomopsis sp. and Aspergillus sp. On Potato Dextrose Agar, the colony of Phomopsis
sp. formedwhite miselium, texture floccose, filamentous, margin filiform and elevation umbonate;
hyphae hyaline, septate; conidia oval to fusoid; and produced yellow-orange soluble pigments.
Mycelium with pink concentric ring are formed after 5 - 6 days old culture and umbonate mass of
mycelium in the center of colony are formed after 23 - 30 days old culture. The colony grow at a rate
10,25 mm per day. The colony of Aspergillus sp. formed white miselium, texture floccose, filamentous,
margin filiform and elevation raise; hyphae hyaline, septate; conidiophore with inflated apical cell and
thin in cell wall; spore smooth, rounded, and hyaline. Pale yellow mass in the center of colony are
formed after 5 days old culture. Soluble pigment is absent. The colony grow at a rate 9,5 mm per day.
Visual growth colony of dual cultures Phomopsis sp. and Aspergillus sp. shows contact inhibition with
no antibiosis.
Keywords: endophytic fungi, Gyrinops versteegii, Phomopsis, Aspergillus, Lombok

oobverse reverse obverse reverse
(b) (e)
(d) (g)
(a)
(c) (f)
Fig. Morphology appearence of endophytic fungi on Potatoe Dextrose Agar. Phomopsis sp. (a) 6 days culture, (b) hyphae and (c) conidia. Aspergillus sp.
(d) 6 days culture, (e) hyphae and (f) conidiophore. (g) Phomopsis sp. and Aspergillus sp. dual cultures.
References:
[1] M. Sangareswari, K.T. Parthiban, S.U. Kanna, L. Karthiba, and D. Saravanakumar. American J Plant Sci 7: 1445-1452, (2016).
[2] A. Danggomen, N. Visarathanonth, L. Manoch and O. Piasai. Thai J Agri Sci 46(3): 157-164, (2013).
[3] T. Mulyaningsih and I. Yamada, “Notes on some species of agarwood in Nusa Tenggara, Celebes and West Papua, ” in Natural Resource Management
and Socio-Economic Transformation Under The Decentralization in Indonesia: Toward Sulawesi Area Studie. CSEAS, Kyoto, (2008).
[4] S.K. Deshmukh, M.K. Gupta, V. Prakash, and S.Saxena. J Fungi4(77), (2018).
1st International Symposium on Agarwood and Aromatic Plants 2019 65

Golden Palace Hotel Mataram, October 1-2, 2019
OP-I7
Nutrient Injection for Agarwood Formation
Supriyanto
SEAMEO BIOTROP, BOGOR

Department of Silviculture, Faculty of Forestry, IPB-University
Corresponding E-mail: supriyanto@biotrop.org
Abstract
Agarwood is known as valuable non-timber forest products, especially its essential oil for
different usages. The fact, the existence Aquilaria sp producing agarwood from forests is becoming
difficult to obtain. Therefore, an effort for producing agarwood from plantation using artificial means is
crucially needed. Organic nutrient injection was used to feed the endophytic microbes forming
agarwood. The objective of this research was to study the effects of nutrient injection on agarwood
formation. Organic nutrients were injected into Aquilaria filaria of 19 years old. The organic nutrient
consisted of molasses (20%), citronella oils (5%), coconut water (25%), agarwood hydrosol (10%) and
date palm vinegar (10%), jasmine oils (5%), and distillate water (25%). Nutrient pH and sugar content
was adjusted to 4.5 and 15% brix prior to injection. Injection holes were made using boor machine of 2
mm in diameter until 1/3 of diameter depth. Horizontal distance of injection holes was 4 cm, and 10 cm
for vertical distance. Organic nutrients were injected every month for 6 months. The harvested agarwood
was distillated using cohobation methods and analyzed using GC-MS. The results showed that organic
nutrient injection for 6 months was able to induce the agarwood formation and essential oils. Some
endophytic fungi was also developed surrounding the injection holes. The GC-MS analysis showed that
the main chemical component in agarwood was 1,2 Benzenedicarboxyl acid, dioctyl ester (CAS) dioctyl
phthalate (DOP) found in brown withes and dark brown agarwood, 93.29% and 91.35%, respectively.
It is possible that those compounds plays important role in forming agarwood in longer period. The
burning agarwood produced light agarwood smell, but Agarospirol compound was not detected in GC-
MS analysis.

TISSUE CULTURE, ECOLOGY,
BIOSISTEMATIC, MANAGEMENT OF WILD
AND PLANTING TECHNOLOGY OF
EAGLEWOOD AND AROMATIC PLANTS

OP-T1
Elevating Agarwood Fragrance Sesquiterpenes Production in Aquilaria
filaria Suspension Cultures
Erna Listiana1, Sumarjan1, Ferdinand Schroeder2, Ulrich Schurr3, Tri Mulyaningsih4
1 Faculty of Agriculture, Mataram University, Jl. Majapahit No. 62, Mataram 83127, Indonesia.
2Symrise AG, Muehlenfeldstrasse 1, 37603 Holzminden, Germany.
3 Institute of Plant Science, Juelich Research Center, Wilhelm-Johnen-Strasse, 52428 Juelich, Germany.
4 Faculty of Mathematics and Natural Sciences, Mataram University, Jl. Majapahit No. 62, Mataram 83127,
Indonesia.
Corresponding E-mail: e.listiana@unram.ac.id
Abstract
Agarwood, a scented oleoresins formed in the heartwood of Aquilaria sp. has become precious
non-timber commodity from South East Asia forest for centuries. High value of agarwood derived from
woody, warm, sweet, nutty or spicy scented characters of sesquiterpenes (SQTs), namely agarofuran,
jinkohol, guaiene, etc. The worth of agarwood is the reason of uncontrolled logging of Aquilaria plant
in their native habitat which leads the inclusion of many plant species of this genus into the red list of
threatens species by IUCN (International Union for Conservation of Nature). Perfumery industries
which use agarwood as a raw material in their production process concern to the growing need of
consumer in environmentally friendly product with sustainable development.
As natural agarwood plant become extinction, this research aims to produce agarwood high
valuable SQTs from A. filaria cells suspension cultures. The technique employs elicitation using yeast
extract elicitor and sucrose as a carbon source. The result revealed both yeast extract elicitor and
additional sucrose feeding induce the synthesis of jinkohol and acorenone. Besides, the addition of yeast
extract elicitor and increasing concentration of sucrose enhance significantly the synthesis of jinkohol
and acorenone. Long term cell suspension culture experiment confirmed that jinkohol and
acorenoneinitially detected at eleven days of culture. The prevalence SQTs emitted from A. filaria
suspension cultures were α-guaiene, δ-guaiene and α-humulene.
Keywords: elicitation, perfumery, sucrose, yeast extract.
References:
[1] Chattopadhyay, S., Farkya, S., Srivastava, A.K. and Bisaria, V.S. Biotechnol. Bioprocess Eng, 7:
138-149 (2002).
[2] Naef, R., 2011. Flavour and Fragrance Journal, (26) (2): 73-87 (2011).
[3] Panten, J., Bertram, HJ., and Surburg, H. Chemistry and Biodiversity, (1): 1936-1948 (2004).
[4] Pichersky, E. and Gershenson, J. Current Opinion in Plant Biology,(5):237-243 (2002).
[5] Pripdeevech, P., Khummueng, W. and Park, S.K. Journal of Essential Oil Research, (23): 46-53
(2011).

OP-T2
Production of Agarwood Constituents by Callus Cultures of Gyrinops
Agus Sukito1* and Cecep Handoko1
1Research and Development Institute of Non-Timber Forest Product Technology, Ministry of Environment and
Forestry
Jl. Dharma Bakti No.7, Lingsar, Lombok Barat District, Nusa Tenggara Barat Province - Indonesia
Corresponding authore-mail: sukitokun@gmail.com
Abstract
Agarwood was as known a material containing the resin, parfume, and bioactive compounds that
produced by agarwood plant producer. Among several plant producer, Gyrinopsversteegii is the one
where endemic at Nusa Tenggara Barat Province. Agarwood containing chromone and sesquiterpene
derivatives as the main constituents using for cosmetics and perfume [1][2][3]. The aim of this research
is to investigate the production of agarwoodconsituentsby callus induction ofG. versteegii. As the
results, the threatment of MS medium with combination of growth hormone 2,4-D:kinetin (1:1 and 2:1,
respectively) show the callus induction. The average of initial callus was induced at 25 days of
incubation time with compact texture and indicated brownish yellow color. LC-MSMS analysis showed
that callus produced 2,2-phenylethylchromone and α-humulene compounds.
Keywords: callus cultures, agarwood constituents, Gyrinopsversteegii
References:
[1] T. Nakanishi, E. Yamagata, K. Yoneda andI. Miura, Phytochem.,20: 1597–1599 (1981).
[2] Y. Shimada, T. Tomoninaga, T. Konishi and S. Kiyosawa, Chem Pharm Bull, 30: 3791– 3795
(1982).
[3] T. Nakanishi, E. Yamagata, K. Yoneda, I. Miura and H. Mori, J ChemSoc Perkin Trans, 1: 601–
604(1983).

kinetin:2,4D=1:1 kinetin:2,4D=1:2
7
6
Callus growth [Ln A (mm2)]
0
0 15 30 45 60 75 90
Day
Figure 1. Callus growth of G. versteegii after addition with the combination of hormone growth (2,4-
D:kinetin=1:1 dan 2,4-D:kinetin=2:1)after 90 days incubation time

OP-T3
Development of Agarwood Producing Tree in Riau Province (Case Study:
Agarwood Plantation in Siak Sri Indrapura District and Kampar District)
Yulizah, Agung Adi Nugroho, and Joeni Setijo Rahajoe
Research Center for Biology, INDONESIAN INSTITUTE OF SCIENCE Cibinong Science Center, Jl. Raya
Jakarta Bogor KM 46, Cibinong, West Java 16911
Corresponding author E-mail: yulizah.rhiezha@gmail.com
Abstract
Cultivation is one of the conservation methods that is very important for the survival of agarwood-
producing trees. In the Province of Riau, cultivation of agarwood has been developed since 2012,
concentrated in Siak Sri Indapura and Kampar Districts. This study aims to determine the pattern of
development and ecological conditions of agarwood trees land and economic evaluation of agarwood
cultivation by comunity in Riau Province. Each location of agarwood cultivation is directly observed
(descriptive) including spacing, types of plants, physical condition of plants, and soil sampling to
determine soil nutrient elements. The species of agarwood-producing trees found is
Aquilariamalaccensis which is found in 7 locations of agarwood cultivation with agroforestry patterns,
where the agarwood- producing trees are mixed with palm oil, rubber and other fruit crops. Climatic
conditions at the cultivation site have an average temperature of 35 OC, with 57% humidity. The average
soil pH of 6.2 is acidic, while the soil nutrients P, Ca, K, Mg, Na, N and C in each location do not show
a significantly different. Economic evaluations from planting to obtaining results do not show significant
benefits because they are still constrained by inoculation techniques that have not yet developed and the
difficulty of obtaining the best inoculant producing agarwood. The development of cultivation
techniques of agarwood-producing plants must be standardized and can be widely applied so that people
are motivated to cultivate agarwood- producing trees.
Keywords: Aquilaria malaccensis, Agarwood Plantation, Gaharu, Riau, Soil nutrients,
Table 1. Development Patter and the condition of agarwood plantations in Riau province
Number of Age Average Average
Locations Area (ha) Owner Type
plants (years) diameter height
Bangkinang, Kampar 6 4000 7 13.05 7.05 Yahya Agroforestry
Air Tiris, Kampar 1 1000 4 8 4 Bahrum Monoculture
Sebak Auh, Siak 2 180 6 17 6 Riki Agroforestry
Siak Kecil, Siak 5 80 7 16 7 Supiadi Agroforestry
Bunga Raya, Siak 2 1000 13 21 13 Yasin Agroforestry
Tuah Inda Pura, Siak 3 1200 10 15 7 Rahmat Agroforestry
Tuah Inda Pura, Siak 2 300 7 9.5 6 Tukino Agroforestry

References:
[1] Soehartono T, Newton AC. 2000. Conservation and sustainable use of tropical trees in the genus
Aquilaria I. status and distribution in Indonesia. Biol Conserv 96: 83-94.
[2] Soehartono T, Newton AC. 2001. Conservation and sustainable use of tropical trees in the genus
Aquilaria II. The impact of gaharu harvesting in Indonesia. Biol Conserv 97: 29-41.
[3] Turjaman M, Hidayat A., Santoso E. 2016. Development of agarwood induction technology using
endophytic fungi. In: Mohammed R (eds). Agarwood, Science Behind the Fragrance. Springer
Science+Bussiness Media, Singapore.
[4] Situmorang, J. 2000. Mikropropagasi Kayu Gaharu (Aquilariaspp) Asal Riau Serta Identifikasi
Sifat Genetiknya Berdasarkan Analisa Isoenzim (Tesis). Program Pasca Sarjana, InstitutPertanian
Bogor (IPB), Bogor.
[5] Sumarna, Y. 2002. Budidaya Gaharu. Penebar Swadaya, Jakarta.
[6] Kanazawa, K. 2017. Sustainable Harvesting and Conservation of Agarwood: A Case Study from
the Upper Baram River in Sarawak, Malaysia. TROPICS. 25(4):139-146.
[7] Elias, M. Fauzhi, Husni Ibrahim, Wan Rusmawati Wan Mahamod. 2017. A Review on the
Malaysian Aquilaria species in Karas Plantation and Agarwood Production. International Journal
of Academic Research in Business and Social Science. 7 (4) : 1021- 1029.

OP-T4
Ecological and Economical Assessment of Agarwood Plantation in Central
Bangka, Indonesia
Thifali Adzani, Koji Matsushita, Mamoru Kanzaki
Division of Forestry and Biomaterials Science, Graduate School of Agriculture, Kyoto University Kitashirakawa
Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan
Corresponding author e-mail: thif.adzani@gmail.com
Abstract
Agarwood is a fragrant resinous wood mainly used in incense and perfume. Agarwood plantation
business emerged in tropical countries from 1990 because of decreasing wild populations and its high
economic benefit. Community and Government in Central Bangka Regency, Indonesia, established
agarwood plantations since 2001. However, after the planting activities, there was no expansion of the
plantation business in the area. One of the reasons of this situation is the lack of scientific evaluation of
the plantation business. This study aims to evaluate the plantation from ecological and economical
viewpoints. The study consists of the establishment of the ecological simulation model of plantation
forest growth and products and the financial model based on the plantation growth model in Central
Bangka.
The study conducted at the mixed plantations of agarwood (Aquilaria malaccensis) and rubber
wood which is the most common plantation designin the area. Twelve trees, including fourtrees
inoculated with microbes to enhance resinous wood formation, were felled to make allometry equation
of agarwood biomass to estimate the above ground biomass and agarwood products; leaves for tea, non-
resin wood for oil and powder and the resinous wood weight. Resinous wood's percentage were
estimated using scanned image of stem disc samples of the inoculated sample trees. The economical
evaluation was assumingthe several harves tingschedules and financial feasibility was determine dusing
NPV, BCR, and IRR. The simulation was repeated by changing the assumption on resin ous wood
quality and all the non-resinous wood converted into oil and powder.
The best allometry of stem weight (Ws) was Log10Ws = 0.8203 Log10 (dbh2 H) - 1.3672. The
weight of other part of trees also estimated using the power-function allometric equations. From a
hectare of the mixed agarwood rubber wood plantation, resinous wood product was estimated to be 0.80
Mg/ha, agarwood oil 6.91 L/ha, leaves product 0.81Mg/ha, and powder 2.3 Mg/ha, at 11 year plantation.
For simulation if the resinous wood is sold, NPV at year 11 was Rp.1,539,590,185 (¥ 11,842,924). The
IRR was 87%, B/C ratio was 3.20. The simulation results for all the option was feasible financially.
Even the case when all the wood used to produce oil and powder and no resinous wood was collected
also generated income to the farmer in our simulation. Good inoculant possibly increases the amount of
resinous wood and income for the farmers. Our results are a prediction from the simulation models and
need to be confirmed by the actual harvestdata.
Keywords: agarwood plantation, Aquilaria malaccensis, allometry, Central Bangka.

OP-T5
Phylogenetic Study of the Indonesian Agarwood using Nuclear and
Chloroplast DNA
Marlina Ardiyani1, Lulut D. Sulistyaningsih1, Wita Wardani1, Kusumadewi S. Yulita1, Lina S.

Juswara1, Seni K. Senjaya1, Rani Asmarayani1, Susila1
1Herbarium Bogoriense, Botany Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI),
Jl. Raya Bogor Km. 46, Cibinong 16911, INDONESIA
Corresponding author e-mail: marlina.ardiyani@gmail.com
Abstract
The agarwood species of Aquilaria and Gyrinops are listed in Appendix II CITES. In Indonesia,
they consisted of 13 species distributed from Sumatra to Papua. This study aimed to examine the
phylogenetic relationship of the Indonesia agarwood. We use nuclear as well as chloroplast DNA.
Internal Transcribed Spacer (ITS) was used for the nuclear data. While four regions of chloroplast ie.
rbcL, matK, trnL-trnF intergenic spacer and trnH-psbA intergenic spacer were used in the study.
Phylogenetic analysis showed that the ITS and trnL- F data produced more resolved trees, while the data
from other regions showed many polytomies. All data showed that Aquilaria and Gyrinops were
paraphyletic. Maximum Likelihood (ML) analyses of the ITS and trnL-F data resulted in a phylogenetic
tree with similar topologies. Gyrinops walla and Aquilaria khasiana from Ceylon and India are basal
species close to the outgroup. ML analysis of the trnL-F data resulted in a tree with five clades (G.
walla-A. khasiana clade; A. crassna-A. subintegra-A. rostrata clade; A. beccariana-A. hirta-A.
malaccensis-A. microcarpa clade; A. yunnanensis-A. sinensis-A. rugosa clade; and Gyrinops spp.-A.
filaria-A. cumingiana-A.urdanetensis clade). Whereas ML analysis of the ITS data resulted in a tree
with 6 clades (G. walla clade; A. crassna-A. subintegra-A. sinensis clade; A. rugosa-A. yunnanensis
clade; A. cumingiana-G. versteegii clade; Gyrinops spp. clade; and A. beccariana-A. hirta-A.
malaccensis-A. microcarpa clade). The clades in the ITS and trnL-F tree has a unique geographic
distribution. A. sinensis, A. rostrata, A. cumingiana and G. versteegii were placed in different clades in
the tree based on ITS and trnL-F data. The rest of the species of the Agarwood were embedded in the
same clades in the tree based on both molecular data. The Indonesian agarwood are not monophyletic
and all of them are sister to the species from Ceylon or India.
Keywords: Aquilaria, Gyrinops, Internal Transcribed Spacer (ITS), trnL-F.

Figure 1. Phylogenetic tree from Maximum Likelihood analysis of the Internal Transcribed Spacer
(ITS) data set.

Figure 2. Phylogenetic tree from Maximum Likelihood analysis of the trnL-F data set.

References:
[1] Ding Hou. Blumea 12: 285–288 (1964).
[2] Ding Hou. Wolters-Noordhoff Publishing, Groningen, The Netherlands. In: Van Steenis
[3] C. G. G. J. (eds.) Flora Malesiana 1(6): 1–48 (1960).
[4] Eurlings, M.C.M. & B. Gravendeel. Plant. Syst. Evol. 254:1-12 (2005).
[5] Feng, T., Li, Q., Wang, Y., Qiu, S., He, M., Zhang, W., Dong, J., Zhu, S. Holzforschung
[6] 73(6): (2018).
[7] Lee, S.Y., Turjaman, M., Mohamed, R. Tropical Life Sciences Research 29(2): 13-28 (2018).
[8] Tamura, K., G. Stecher, D. Peterson, A.Filipski & S. Kumar. Molecular Biology and Evolution 30:
2725‒2729 (2013).

OP-T6
Strengthen Farmer on Agarwood BMW Marketing Chain
Rubangi Al Hasan1, Amalia Indah Prihantini2&Mansyur3
123 Non-Timber Forest Product Technology Research and Development Institute
Jl. Dharma Bhakti No. 07, DesaLangko, Kec. Lingsar Kab. Lombok Barat, West Nusa Tenggara
Correspondent: rubhasan@yahoo.com
Abstract
Black Magic Wood (BMW) is the residual product of agarwood that has not received much
attention. Even though in terms of production quantity in tree units, BMW actually has the highest
quantity. This study intends to examine the parties involved in BMW trading and who has the dominant
role. This study concludes that agarwood farmers are the smallest parties to get the biggest burden and
get the smallest profit. The price received by farmers is very low, inversely proportional to the level of
costs incurred. The second party with a small profit level is the agarwood craftsman. These craftsmen
only get wages from the work of producing BMW raw materials. The party who gets the biggest profit
is the big trader. From the investation point of view it is indeed large, but the benefits obtained are far
greater than the investation incurred. In trading practice, there are two levels of dominance. In the field,
traders of BMW materials collectors are very dominant in determining the prices of BMW raw materials.
Farmers have a small bargaining position in BMW mareting chain. Meanwhile at the BMW market
level, large traders have a large degree of dominance in determining the products should be produced
by BMW craftman via BMW collectors. For this reason, efforts are needed to build a more equitable
marketing chain. The solution includes building stronger institutions at the farm level. Agarwood
farmers need to build farmer groups to strengthen farmer institutions and improve their bargaining
position in front of other parties. Next, it is necessary to build a farmer cooperative to accommodate
BMW's agarwood production independently. Thus farmers do not sell in the form of tree stands, but
already in the form of BMW raw material products. This effort is also to cut the trading chain so that it
is shorter. Farmers who are members of a cooperative can directly sell to large traders so that they get
greater profits with a better bargaining position.
Keywords: black magic wood, farmer, marketing chain, agarwood.

ABSTRACT
POSTER PRESENTATION

BIODIVERSITY, BIOSYSTEMATICS,
ANATOMY, ETHNOBOTANY, ITS SYMBIONTS
OF AGARWOOD AND AROMATIC PLANTS

P-E1
Species Identification of Agarwood using a DNA Barcoding Method
Seiji Tanakaa, Michiho Itoa
aGraduate School of Pharmaceutical Sciences, Kyoto University Corresponding author E-mail:

michihoi@pharm.kyoto‑u.ac.jp
Abstract
Source plants of agarwood are continuously decreasing their number in wild, and they were listed
in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora
in 2005; however, these source species are hardly identified by morphological characteristics of leaves
and trunks.
According to the Japanese Standards for Non-Pharmacopoeial Crude Drugs 2018 (Non- JPS
2018), five species in the genus Aquilaria have been designated as source species for agarwood. In order
to identify source species without morphological characteristics, a DNA barcoding method for agarwood
was developed. Agarwood pieces derived from Gyrinops species were found in the Japanese market
using this method and reported in our previous report.
In this study, the barcoding method was applied to collected plant materials and commercial
agarwood samples from Sumbawa, Lombok, Sulawesi, and Kalimantan islands in Indonesia. As a result,
it was turned out that source plant species were different from island to island and both plants of
Aquilaria and Gyrinopswere found among the samples. Gyrinops plants are not currently listed as source
species for agarwood in Non-JPS 2018, though, they shall be added to the list for source plant according
to this result. Appropriate expanding of source species for agarwood may help reducing a risk of
depletion of the resources.
Keywords: DNA barcoding, Aquilaria, Gyrinops
Table 1. Genotype of main agarwood source species based on alignment of the trnL-trnF region
Single NucleotidePolymorphisms
List of
agarwood source species 291 301 327 328 339 368 381
159
C T T T - G C A
Aquilariamalaccensis
A T T - - G C A
A. microcarpa
A G T T T T A A
A. sinensis
A T G T - T C C
A. crassna
A T T - - T C A
A. khasiana
A T T T - T C A
Gyrinopsversteegii
A G T T T T C A
G. ledermannii
A T T T T T C A
G. moluccana

Fig. 1 Geographical separation of Indonesia based on the Wallace Line and Weber Line
Fig.2 Examples of morphology of agarwood samples used in this study
Reference
[1] S. Tanaka, M. Ito, Journal of Natural Medicines,https://doi.org/10.1007/s11418-019- 01338-z
(2019), (Online First).
[2] CONVENTION ON INTERNATIONAL TRADE IN ENDANGERED SPECIESOF WILD
FAUNA AND FLORA, ANNUAL REPORT OF THE SECRETARIAT,
https://www.cites.org/sites/default/files/document/2005-06.pdf, accessed August 26th 2019.
(2006)
[3] The Ministry of Health, Labour and Welfare, The Japanese standards for non- Pharmacopoeial
crude drugs 2018, https://search.e- gov.go.jp/servlet/PcmFileDownload?seqNo=0000177757,
accessed August 9th 2019, (2018).

P-E2
Species of Eaglewood Weed (Cyperaceae) in Mother Plants Garden of Eaglewood
Pusuk, West Lombok, Indonesia.
Wiwin Hidayat1 and Tri Mulyaningsih2*)
1Plant Biodiversty Club, Faculty of Mathematics and Natural Sciences, Universitas Mataram, Lombok, West
Nusa Tenggara, Indonesia.
2Faculty of Mathematics and Natural Sciences, Universitas Mataram, Lombok, West Nusa Tenggara, Indonesia.
*)Correspondence author e-mail: trimulya@unram.ac.id
Abstract
Cyperaceae is a large family of Liliopsida class, which is consisted of 5000 species in 120 genera.
These plants can be used as an antivenom, antimicrobial, antimalarial, hepatoprotective, hypoglycemic,
diabetes and diarrhea. This research was conducted on August 21 to September 21, 2019 which was
held in the Eaglewood Mother Plants Garden Area Pusuk, West Lombok, West Nusa Tenggara,
Indonesia. This research was conducted on August 21 to September 21, 2019 which was held in the
Eaglewood Mother Plants Garden Area Pusuk, West Lombok, West Nusa Tenggara,
Indonesia.Collecting samples were used line sampling method.
The results of this study were obtained 6 species in 3 genera, that were found under the stand of
aloeswood trees. These species are Cyperus albostriatus Schard.,C. esculantus L., Cyperus sp., Kyllinga
brevifolia (Rotb.) Endl., K. nemoralis (Hutch. & Dalz.) Dandy ex Hutch. & Dalziel, and Scleria
sumatrensis Retz.
Keywords: habitat, Cyperaceae, Cyperus, Kyllinga, Gryinops versteegii.
References:
[1] Goetghebeur, P. 1998. Cyperaceae. In K, Kubitzki, H Huber, P. Rudall, P Stevens, T Stutzel (eds).
The families and genera of vasculer plant. Flowering plants- Monocotyledons. Springer-Verlag,
Berlin. Vol.4. Pp: 141-190.
[2] Govaerts, R., D.A. Simpson., J. Bruhl., P. Goetghebeur., K.L. Wilson., and T. Egorova. 2007.World
Checklist of Cyperaceae. London: Royal Botanic Gardens, Kew.
[3] Pl@ntNet. 2019. Cyperaceae. http://www.plannet-project.org. Accessed on August 24, 2019.
[4] Simpson, D.A., C. Yesson., A. Kulham., C.A. Couch., A.M. Mausaya. 2011. Cilmate change and
Cyperaceae (Chapter 19). Cambridge University Press. Pp.: 439-456.
http://centaur.reading.ac.uk/24719/.
[5] The Plant List. 2013. Cyperaceae. http://www.theplantlist.org. Accessed on August 24, 2019.

P-E3
Stem Anatomical Variation of the Genus Gyrinops (Thymelaeaceae) in
Sulawesi, Indonesia
Karmilawati¹, Tri Mulyaningsih1*), Aida Muspiah¹, Michiho Ito2
1Departement of Biology, Faculty of Mathematics and Natural Sciences, University of Mataram Jl. Majapahit
No. 62, Mataram, Indonesia
2Graduate School and Faculty of School of Pharmaceutical Sciences, Kyoto University, Japan.
*)email: trimulya@unram.ac.id
Abstract
The research on trees of producing agarwood can be carried out based on their anatomical
characteristics to help on identifying agarwood based on the eaglewood species, so that it can be used
as a key character for classification based on the anatomical evidence. Agarwood producing trees are
included to Thymelaeaceae family, one of their genus is Gyrinops. Stem anatomical structure producing
of agarwood trees species of Gyrinops has different shapes, sizes and arrangements. The purpose of this
study was to determine the variation of the stem anatomical structure (i.e. cortex: sclerenchyma, phloem;
xylem: trachea, rays, interxylary phloem and wood parenchyma; and pith) of Gyrinops (Thymelaeaceae)
in Sulawesi.
The method of this research was descriptive exploration. Stem agarwood sample came from several
regions areas of Sulawesi which were collected by Agarwood Research Center Laboratory, Faculty of
Mathematics and Natural Sciences, University of Mataram. The slide methods were used permanent
slides (paraffint) and fresh slides.
The results of this research showed there were1 species of Gyrinops decipiens Ding Hou and 3
species of Gyrinops sp., with various anatomical characters in G. decipiens and Gyrinops sp. in terms
of the position, diameter and percentage of pith thickness; sclerenchyma cortex pattern and arrangement
of wood parenchyma. There were also variations in cortex thickness; diameter, frequency and
arrangement of trachea; frequency of rays; diameter, and interxylary phloem frequency; pith diameter;
and the position of Ca- Oxalate in Gyrinops sp.3 with Gyrinops sp. 1 and 2, while Gyrinops sp.2 with
Gyrinops sp.1 there were found 5 variations such as the diameter, frequency and arrangement of trachea;
arrangement of rays and interxylary phloem length.
Key words: Agarwood, Gyrinops, interxylary phloem, plant anatomy, Sulawesi
References:
[1] Andianto. 2010. Anatomical Features of Five Species Producing-Eaglewood And Two Related
Species.Journal of Forest Products Research. 28(2): 169- 183.
[2] Angyalossy, V., M.R. Pace & P. Baas (eds.). 2016. IAWA List Microscopic Bark Features.
IAWA Journal. 37(4): 517–615.
[3] Asdar. 2006.The Anatomical Characteristics of Eagle Wood (Gyrinops versteegii(Gilg.) Domke)
from Gorontalo. Perennial Journal. 3(1): 6-10.
[4] Chalk, L. & M.M. Chattaway. 1937. Identification of wood with included phloem. Tropical wood.
50: 1-31.
[5] Hou, D. 1960. Thymelaeaceae. In: Van Steenis C.G.G.J. (ed.) Flora Malesiana. 6 (1): 1-

160.Wolters-Noordhoff Publishing. Groningen.
[6] Luo, B., Y. Ou., B. Pan., J. Qiu & T. Itoh. 2018. The structure and development of interxylary
and external phloem in Aquilaria sinensis. DOI 10.1163/22941932-20170182. Published by
Koninklijke Brill NV, Leiden. IAWA Journal. 39 (1): 3–17.
[7]. Mandang, Y.I & B. Wiyono. 2002.Anatomy of Eagle Wood (Aquilaria malaccensis Lamk.)and
Several Related Species. Forest Products Research Buletin. Forestry Engineering and Forest
Products ProCessing Research and Development Center, Forestry Research and Development
Agency. Bogor.
[8]. Mohamed, R.(ed.). 2016. Agarwood Science Behind the Fragrance. Springer Nature. Singapore.
DOI 10.1007/978-981-10-0833-7.
[9]. Mulyaningsih, T., D. Marsono., Sumardi & I. Yamada. 2014. Selection of Superior Breeding
intraspecies Gaharu Gyrinops versteegii (Gilg.) Domke.Journal of Agricultural Science and
Technology. 4(2): 485-492.
[10]. Mulyaningsih, T. & I. Yamada. 2007. Notes on Some Species of Agarwood in Nusa Tenggara,
Celebes and West Papua. Faculty of Agriculture, Mataram University -Professor Emeritus, Kyoto
University.
[11]. Mulyaningsih, T., Parman & Sumarjan. 2005. Increasing of bio-proCessing agarwood with
synergisms of some fungi isolate on Gyrinops versteegii (Gilg) Domke, in: Article National
Seminar of Fundamental Research Result. Department Higher Education Directorate of National
Education. Jakarta.
[12]. Mulyaningsih, T., S. Hadi & Sumarjan. 2004. Influence of fungi isolates on the quality of
production and bioactive content of agarwood on the ketimunan trees (G. versteegii (Gilg.)
Domke). In: Paper of National Seminar of Fundamental Research Result, Department Higher
Education Directorate of National Education. Jakarta.
[13]. Mulyaningsih, T. & Sumarjan. 2002. Formation Interxylary Phloem and Aromatic Resin In
Gyrinops versteegii (Thymelaeaceae). IAWA Journal. 23(24): 472-473.
[14]. Mulyaningsih, T., Sumarjan & Parman. 2007. Influent of light intensity to anatomy of agarwood
made, yearly ring and new tissues on around inoCulation holes at eaglewood tree
Gyrinopsversteegii (Gilg) Domke. National Development Planning Agency. Vol.1 No.112/113.
[15]. Suharti., Mukarlina & D. Gusmalawati. 2017. The anatomical structure of the root, stem and leaf
of the agarwood (A. malaccensis) that experiences drought stress. Protobiont. 6 (2): 38-44.
[16]. Wheeler, E.A., P. Baas & P.E. Gasson (eds.). 1989. List of Microscopic Features For Hardwood
Identification with an Appendix on non-anatomical information. IAWA Bulletin. 10(3): 219–882.
[17]. Widoyanti, Prasetyorini & Ismanto. 2017. Differences in Anatomical Structure of Agarwood-
Producing Plants fromAquilaria spp. and Gyrinops versteegii. Ekologia. 17(1):1-6.

P-E4
Diversity of Ferns in under the Stand of Gyrinops versteegii in the Mother
Plants Garden of Eaglewood Pusuk, West Lombok, Indonesia
Lukmanul Hakim1, Ni Nengah Sri Eni1 and Tri Mulyaningsih1*)
Department of Biology, Mathemetics and Natural Sciences Faculty, UniversitasMataram, Indonesia

*) Correspondent author e-mail: trimulya@unram.ac.id
Abstract
Gyrinops versteegii (Thymelaeaceae) has association plants, one of them is ferns. This paper
aimed to discover the diversity of ferns that grew in the Mother Plants Garden of Eaglewood Pusuk,
West Lombok. This research was conducted on July – August 2018. The specimens sampling were used
census method. The fern specimens were taken from all the area of under the stand of G. versteegii in
the Mother Plants Garden of Eaglewood Pusuk, West Lombok.
The result showed that there are 10 species of ferns which belong to 7 families of ferns:
Selaginellaceae (Selaginella plana), Tectariaceae (Tectariaauritaand Tectariaangulata), Woodsiaceae
(Diplaziumesculentum), Thelypteridaceae (Cyclosorus parasitic and Cyclosorusdentatus), Lygodiaceae
(Lygodiumcircinatum), Oleandraceae (Nephrolepiscordifolia), and Pteridaceae (Pteris biaurita and
Pteris ensiformis).
Keywords: agarwood, Gyrinopsversteegii, ferns, Lombok.
References:
[1] The Plant List. 2013. http://www.theplantlist.org. Accessed at May 18, 2019.
[2] Retrieved [May 30, 2019], from the Integrated Taxonomic Information System on-line database,
http://www.Itis.gov.
[3] Walkowiak, R. J. 2017. Classification of Pteridophytes. International Equisetological Association
Paper.
[4] Mulyaningsih, T., D. Marsono, I. Yamada. 2016. Community of eaglewood Gyrinopsversteegii
(Gilg.) Domke and the diversity of plant species associated in western Lombok forest. Proceeding
ICST 2015: 361- 382.

P-E5
Comparative of Stem Anatomy in Genus Gyrinops (Thymelaeaceae) onthe
Sumbawa Island
Zainul Muttaqin Sany1, Tri Mulyaningsih1*), Aida Muspiah1, Isamu Yamada2, Michiho Ito3
1Biology Study Program, Faculty of Mathematics and Natural Science, UniversitasMataram, Indonesia.
2Center for Southeast Asian Study, Kyoto University, Japan.
3Graduate School and Faculty of Pharmaceutical Sciences, Kyoto University, Japan.
*)Corresponding author email:trimulya@unram.ac.id
Abstract
The aim of this research is to determine the comparative anatomy of the stem (cortex tissue,
xylem, pith, and ergastic objects (Ca-Oxalate)) in the genus Gyrinops (Thymelaeaceae) on Sumbawa
Island. This research is descriptive exploratory and it was conducted in April - May 2018 at Biology
Laboratory, Faculty of Mathematics and Natural Science, University of Mataram. Stem samples of the
genus Gyrinops are used from collections of the Agarwood Center Laboratory, Faculty of Mathematics
and Natural Science, University of Mataramthat were taken from Sumbawa Island. This research was
using fresh slides, hand section and maceration methods. Observationdata were consistedby cortex
tissues (periderm, sclereid, cortex parenchyma, scelerenchyma), xylem tissues (trachea, tracheida,
tracheid fibers, interxylary phloem, wood parenchyma, pith radius), pith tissue (pith position, pith cells),
and ergastic (Ca-Oxalate).
The result of this research showed that can be seen there are 4 species from Sumbawa Island, e.g:
Gyrinops versteegii, Gyrinops sp. 1, Gyrinops sp. 2 and Gyrinops sp. 3 (there are 4 variants: Gyrinops
sp. 3a, Gyrinops sp. 3b, Gyrinops sp. 3c, and Gyrinops sp. 3d). The stem comparative anatomy of the
G. versteegii with Gyrinops spp. have differences in periderm thickness, cortex parenchymal diameter,
cortex sclerenchymal pattern, interxylary phloem thickness, wood parenchymal arrangement, tracheal
diameter, and Ca-Oxalate form. The stem comparative anatomy of the Gyrinops sp. 1 with Gyrinops sp.
2 and 3 have differences in the cortex sclerenchymal pattern, tracheal diameter and length, tracheid
length, and interxylary phloem thickness,. Meanwhile, the stem comparative anatomy of Gyrinops sp. 2
and sp. 3 have differences in tracheal frequency, tracheid length of fibers, and pith radius.
Keywords: Agarwood, Gyrinops, stem anatomy, wood fiber, Sumbawa.
References:
[1] IAWA Committee. 1989. List of Microscopic Features Fqor Hardwood Identification. IAWA
Bulletin. 10(3): 219–882.
[2] IAWA Committee. 2016. IAWA List of Microscopic Bark Features. IAWAJournal. 37(4): 528-
573.
[3] Iswantari, W., T. Mulyaningsih., A. Muspiah. 2017. Karyomorfologi dan Jumlah Kromosom
Empat Grup Gyrinops versteegii (Gilg.) Domke. di Lombok. Jurnal Ilmu Kehutanan, (11): 205.
[4] Johansen, D. A. 1940. Plant microtechnique. New York: McGraw-Hill, 523 p.
[5] Liu, P., X. Zhang., Y. Yang., C. Shui., Y. Xu., & J. Wei. 2018. Interxylary phloem and xilem rays
are the structural foundation of agarwood resin formation in the stems of Aquilaria sinensis. .
(hlm. 3-5). Germany: Springer Nature 2018.

[6] Luo, B., Y. O., B. Pan., J. Qiu., & T. Itoh. 2018. The structure and development of interxylary
and external phloem in Aquilaria sinensi. IAWA Journal, 39(1): 3-17.
[7] Mandang, Y. I. 2004. AnatomiPepagan Pulai dan BeberapaJenisSekerabat. Bulletin Penelitian
Hasil Hutan. 22(4): 247-261.
[8] 7] Mandang, Y. I. &B. Wiyono. 2002. AnatomiKayuGaharu (Aquilaria malaccensisLamk.) dan
BeberapaJenisSekerabat. Bulletin Penelitian Hasil Hutan. PuslitbangTeknologi Hasil Hutan
Bogor.
[9] Mulyaningsih, T. 2017. Research Report The Traditional Use of The Agarwood in Japan Funding
by CSEAS Kyoto University. Mataram: UniversitasMataram.
[10] Mulyaningsih, T., A. Muspiah., E. Arianty., S. Hadi., N. Fitriyani., M. Ito., & I. Yamada. 2018.
VariasiKeragamanMorfologiPohonGaharu di NTB, Indonesia. Pepper of 1st International
Conference & Workshop in Bioscience & Biotechnology by Bioscience & Biotechnology
Research Center University of Mataram, 28th-30th November 2018. Mataram: Golden Palace
Hotel.
[11] Mulyaningsih, T., & I. Yamada. 2007. Notes on Some Species of Agarwood in Nusa Tenggara,
Celebes and West Papua. Faculty of Agriculture, Mataram University - Professor Emeritus,
Kyoto University.
[12] Mulyaningsih, T & S.P.Astuti. 2018. Buku Petunjuk PraktikumMikroteknik Tumbuhan Edisi
Revisi II. Universitas Mataram. Mataram.
[13] Mulyaningsih, T., Marsono., Sumardi & I. Yamada. 2014. Selection of Superior Breeding
intraspecies Gaharu Gyrinops versteegii (Gilg.) Domke.Journal of Agricultural Science and
Technology. 4(2): 485-492.
[14] Sustainable Asset Management. 2018. Price of Agarwood Chips. Diakses pada 10 Juni 2019:
https://www.agarwoodprices.com/prices/agarwood-chips.
[15] Yusof, N. A. M., S. N. Tajuddin., A. Hisyam., and N. A. M. Omar. Agarwood Essential Oil: Study
on Optimum Parameter and Chemical Compounds of Hydrodistillation Extraction. Journal of
Applied Science and Agriculture, 10(5): 1-5.

P-E6
Diversity of Epiphytic Ferns Symbiotic on Gyrinops versteegii in Eaglewood
Parental Garden Pusuk, West Lombok Indonesia
Laily Hunawatun Sani1, Janwar Wahyu1, Tri Mulyaningsih1*)
1Department of Biology, Faculty of Mathematics and Natural Sciences, University of Mataram
*) Corresponding author email:trimulya@unram.ac.id
Abstract
Eaglewood required a compatible ecological condition to survive and grow up well. Ecological
conditions may consisted by specific characteristics habitat and its associated biota such as epiphytic
ferns. This paper aimed to discover the diversity of epiphytic ferns that grew on eaglewood (Gyrinops
versteegii) in Eaglewood Parental Garden Pusuk, West Lombok. This research conducted on July –
August 2018. The sampling data used census method. The researchers identified all the species of
epiphytic ferns which were found on eaglewood stem. The result showed that there are 8 species of
epiphytic ferns belong in 3 families. They were consisted by Aspleniaceae (Asplenium nidus and
Asplenium sp.), Polypodiaceae (Belvisia mucronata, Pyrrosia piloselloides, Drynaria sp., and
Polypodium sp.), and Vittariaceae (Vittaria elongata and Antrophyum plantagieum).
Keywords: Eaglewood, Gyrinops versteegii, Ferns, Lombok
References
[1] Brownsey, P. J., D. R. Given, and J. D. Lovis. New Zealand Journal of Botany, 23 (3): 431 – 489,
(1985).
[2] Mulyaningsih, Tri, D. Marsono, and I. Yamada. “Community of Eaglewood Gyrinops versteegii
(Gilg.) Domke and The Diversity of Plant Species Associated in Western Lombok Forest,” in
Proceeding of ICST 2015: 361-382, Indonesia (2015). https://www.researchgate.net, accessed on
April 15, 2018.
[3] Retrieved [August 30, 2019] from the Integrated Taxonomic Information System on-line database,
http://www.itis.gov,accessed on May 18, 2019
[4] The Plant List. 2013. http://www.theplantlist.org., accessed on May 1, 2019.
[5] Walkowiak, R. J. Classification of Pteridophytes. IEA Paper. (2017).

P-E7
Diversity of Aromatic Plants (Zingiberaceae) in Mother Plants Garden of
Eaglewood Pusuk West Lombok Indonesia
Via Febrianti1, Ainun Astrid1 and Tri Mulyaningsih2*)
1Plant Biodiversty Club, Faculty of Mathematics and Natural Sciences, Universitas Mataram, Lombok, West
2Faculty of Mathematics and Natural Sciences, Universitas Mataram, West Lombok, West Nusa Tenggara,
Indonesia.
*)Corresponding author e-mail: trimulya@unram.ac.id
Abstract
Zingiberaceae is the largest tribe of the order Zingiberales, there are 53 genera with more than
1,500 species around the world and most of them are found in tropical forests. This plant is very popular
among the people because Zingiberaceae have distinctive characteristics such as rhizome which contains
essential oil and distinctive aroma therefor this plant often called an aromatic plant. Zingiberaceae is
found in tropical forests that have sufficient humidity with high rainfall. One of the habitats commonly
shelter or under the trees standsuch as the Mother Plant Garden of Eaglewood, Pusuk, West Lombok,
West Nusa Tenggara, Indonesia.This research was conducted on August 21 to September 21, 2019
which was held in the Eaglewood Mother Plants Garden Area Pusuk, West Lombok, West Nusa
Tenggara, Indonesia. Collecting samples used line sampling method.
The results of this study were obtained 9 species belong to 6 genera such as Alpina galangaL.,
Amomum compactumSoland. Ex Maton, Amomum dealbatum Roxb., Amomum sp., Costus speciosus
(J.Koenig) Sm., Etlingira sp.,Hedychium coronarium J.Koenig., Hedychium sp., and Zingiber zerumbet
(L.)Smith).
Keyword: Diversity, Zingiberaceae, Aromatic Plants, Habitat
References :
[1] El-far, AH, MS Hazeem, WAAbdel. 2018. Costus speciosus: Traditional Uses, Phytochemistry,
and Therapeutic Potentials. Pharmacognosy Reviews, 23(12): 120-127.
[2] Hartanto, S, Fitawati, S Nery.2014. Studi Etnobotani Famili Zingiberaceae dalam
KehidupanMasyarakat Lokal di Kecamatan Pangean Kabupaten Kuantan Singingi, Riau.
Biosaintifika. 6(2): 122-132.
[3] Kinho, J.2011. Karakteristik Morfologi Zingiberaceae di Cagar Alam Gunung Ambang Sulawesi
Utara. Info BPK Manado, 1(1): 35-5.
[4] Nikmatullah, M, ML Suroso, M Pipit.2015. Keanekaragaman Zingiberaceae sebagai Konten Materi
Keanekaragaman Hayati di SMA Pandeglang. Biodidaktika, 2(10): 51-60.
[5] Plant@Net. 2019. Zingeberaceae. http://www.plannet-project.org,accessed on August 24, 2019.
[6] The Plant List. 2013.Zingeberaceae. http://www.theplantlist.org,accessed on August 24, 2019.
[7] Zahara, M., HMauziah, Z Rahma.2018. Identification of Zingiberaceae as Medical Plants in
Gunung Cut Village, Aceh Barat Daya, Indonesia. Journal of Tropical Horticulture, 1(1):.24-28.

P-E8
Diversity and Feeding Ecology of Herpetofauna at Eaglewood
Gyrinops Versteegii Conservation Forest of Pusuk, Western Lombok
Yuliadi Zamroni1*, Ahmad Jupri1, Baiq Farista1, I Gede Nano Septian2, Surya Hadi3, Tri
Mulyaningsih1, Michiho Ito4, Isamu Yamada5
1
Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Mataram, West
2
Department of Fisheries Resources Utilization, Universitas 45 Mataram, West Nusa Tenggara,
Indonesia.
3
Chemistry Study Program, Faculty of Mathematics and Natural Sciences, Universitas Mataram,
West Nusa Tenggara, Indonesia.
4
Graduate School of Pharmaceutical Sciences, Kyoto University, Japan.
5
Center for Southeast Asian Studies (CSEAS), Kyoto University, Japan
*Correspondence author: yzamroni@unram.ac.id
Abstract
Herpetofauna which comprised by amphibians and reptiles are important organisms for
stabilization of the faunal community structure in the ecosystems with their act as secondary and tertiary
consumers in food web organization. These taxa groups gave indirect ecosystem services such as disease
control, agricultural pets control, and seeds dispersed. This study collected eleven species of
herpetofauna from eaglewood (Gyrinops versteegii ) conservation forest of Pusuk-Lombok, is
comprised by four species of amphibians (Duttaphrynus melanostictus, Ingerophrynus biporcatus,
Limnonectes dammarmani and Limnonectes kadarsani) and seven species of reptiles (Ahaetulla prasina,
Cyrtodactylus petani, Dendrelaphis pictus, Draco volans, Eutropis multifasciata, Gekko gecko,
Sphenomorpus melanopogon and Sybinopis geminatus). Based on stomach content analysis, both of
these animal groups commonly are insectivorous. Amphibians are general predator which hymenoptera,
coleoptera, orthoptera and chilopoda are the most their important food, whereas the small arboreal reptils
such as skink and gecko are specialis predator which caterpillar of moth and hymenoptera (especially
an ants) are the dominantly of their stomach content.
Keywords: Herpetofauna, Stomach content, Gyrinops versteegii, Conservation, Lombok.
References
[1] De Lang R. 2011. The Snakes of The Lesser Sunda Islands (Nusa Tenggara), Indonesia. Asian
Herpetological Research 2: 46-54.
[2] Heyer WR, Donnelly MA, McDiarmid RW, Hayek LC, Foster MS. 1994. Measuring and
Monitoring Biological Diversity: Standard Methods for Amphibians. Smithsonian Institution Press.
Washington.
[3] Iskandar DT. 1998. The Amphibians of Java and Bali. LIPI, Jakarta
[4] Kurniati H. 2003. Amphibians & Reptiles of Gunung Halimun National Park West Java, Indonesia.
Lipi, Jakarta.

[5] Mushinsky HR. 1987. Foraging Ecology in Snakes: Ed. Mushinsky. Ecology and Evolutionary
Biology. Volume 1. Mac-Millan, New York.
[6] Pal A, Swain MM, Rath S. 2007. Seasonal Variation in The Diet of The Fan- Throated Lizard,
Sitana ponticeriana (Sauria: Agamidae). Herpetological Conservation and Biology 2(2):145-148.
[7] Rahman LN, Kusrini MD, Haneda NF. 2013. Food Preference of The Javan Tree Frog
(Rhacophorus margaritifer) in Mount Gede Pangrango National Park and Cibodas Botanical
Garden, West Java. J Indo Nat His. 1 (1): 37-41.
[8] Santos EM, Almeida AV, Vasconcelos SD. 2004. Feeding Habits of Six Anurans (Amphibia:
Anura) Species in a Rainforest Fragment in Northeastern Brazil. Iheringia Serie Zool. 94 (4): 433-
438.
[9] Somaweera R. 2017. Reptiles & Amphibians of Bali. John Beaufoy Publishing, Malaysia.

P-E9
Spiders Diversity at Eaglewood Gyrinops Versteegii Conservation Forest of
Pusuk, Western Lombok
Lalu Arya Kasmara, I Wayan Suana, Yuliadi Zamroni*
Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Mataram, West Nusa
Tenggara, Indonesia.
*Correspondence author: yzamroni@unram.ac.id
Abstract
Spider is one of the largest arthropod groups. They comprises by more than fourty- four thousand
species including in 114 families and four thousand genera. Spiders have important ecological function
as secondary consumers in the forest ecosystems. This study aimed to determine the diversity of spiders
in eaglewood conservation forest of Pusuk, western Lombok. The research was conducted on April to
August, 2018. The spiders were collected use pitfall traps, sweep nets and aspirators in 30 plots with
width of each plot is 100 m2. The result of this study recorded 292 individuals of spiders including into
60 spesies and 10 families. Family Araneidae have the highest species collect with 21 species members.
The diversity of spiders in this area is moderate without dominant species.
Keywords: Diversity, Spider, Eaglewood, Pusuk Conservation Forest, Lombok
References
[1] Barrion, A.T & Litsinger, 1995, Riceland spider of South and Southeast Asia, international rice
reserch institute, CAB International, Manila Barrion, AT & Litsinger, 1995, Riceland spider of
South and Southeast Asia, international rice reserch institute, CAB International, Manila.
[2] Foelix, R. F, 1996, Biology of spider second edition. Oxford University Press, New York.
[3] Mahalakshmi, R., & Jeyaparvathi, S, 2014, Diversity of spider fauna in the cotton field of
Thailakulam, Virudhunagar District, Tamil Nadu, India, Zoology Studies, Vol. 1:12–18.
[4] Memah, V. V., Tulung, M., Warouw, J., & Maramis, R. R. T. D, 2014, Diversity of spider species
in some agricultural crops in North Sulawesi, Indonesia, International Scientific & Engineering
Research, Vol. 5:70–75.
[5] Platnick, N. I, 2014, The World Spider Catalog, American Museum of Natural History, America.
[6] Suana I. W. & Haryanto, H, 2013, Keanekaragaman laba-laba dan potensinya sebagai musuh
alami hama tanaman jambu mete, Entomology Indonesia, Vol. 10 (1) : 24-30 [Indonesian].
[7] Suana, I .W, 1998, Studi komparatif keanekaragaman laba-laba (araneae) pada empat
komunitas tumbuhan di gunung tangkuban perahu jawa barat, Tesis program pascasarjana
institut teknologi bandung, Bandung [Indonesian].

P-E10
The Utilization of Gyrinops versteegii (Gilg.) Domke on Lombok and
Sumbawa Island: An Ethnobotanical Perspective
Kurniasih Sukenti1*, Sukiman1, Immy Suci Rohyani1, Tri Mulyaningsih1, Surya Hadi1, Michiho
Ito2, Isamu Yamada3
1Biology Department, Faculty of Mathematics and Natural Sciences, Mataram University, Indonesia
2Center for Southeast Asian Studies, Kyoto, Japan
3Graduate School of Pharmaceutical Sciences, Kyoto University, Japan
Corresponding author Email: kurniasihs@unram.ac.id
Abstract
Eaglewood is one of the high-value non-timber forest products in the world including in Indonesia,
where Lombok and Sumbawa are potential producing areas of Gyrinops versteegii (Thymelaceae). The
purpose of this research is to record and describe the various reported uses of Gyrinops versteegii by
local people of the two islands, so that the potential of Gyrinops versteegii can be explored, developed,
and efforts to support its preservation can also be considered in the future. This study used
ethnobotanical methods in general, i.e observation, interviews, documentation, and also a comparison
of related literature. Basically, there are severalreported uses of Gyrinops versteegii on Lombok and
Sumbawa Island, where nurseries and the wood utilization are the most familiar types of use for the
community. It is hoped that this study couldbe a complement information for research and development
of eaglewood or agarwood, not only in Indonesia but also throughout the world.
Keyword: ethnobotany, Gyrinops versteegii, utilization

AGARWOOD INDUCTION AND GRADING
TECHNOLOGY OF AGARWOOD AND
AROMATIC PLANTS

P-I1
Comparison of Constituents in artificial agarwood pieces Made by
Different Methods
Sakura Takamatsu and Michiho Ito
Graduate School and Faculty of Pharmaceutical Sciences, Kyoto University 46-29 Yoshidashimoadachi-cho,
Sakyo-ku, Kyoto 606-8501, Japan
Corresponding author e-mail: michihoi@pharm.kyoto-u.ac.jp
Abstract
Agarwood is derived from resinous part of Thymelaceae plants especially Aquilaria, Gyrinops
and Gonystylus species and are listed as endangered in the appendix of CITES. People in the producing
country such as Indonesia, have been making agarwood by intentional wounding and using chemicals
on cultivated plants. Resulting agarwood varies in quality, although, relationship between methods and
variety of constituents is not known yet. Chromone derivatives, sesquiterpenes and low molecular
weight aromatic compounds are principal compounds detected from agarwood, and we focused on
chromone derivatives in this study.
Methods are as follows: Aquilaria sinensis trees grown in same condition (Yakushima, Japan)
and same period were treated either by iron nails, chemical reagents or other means. Sixty-five month
after the treatment, resinous parts were harvested and extracted by AcOET and then water. Extracts were
analyzed by LC-MS to obtain patterns of chromatograms. Compounds were identified by comparing
retention time and MS patterns with their standard compounds. Further, artificial agarwood purchased
at market of agarwood producing countries were also analyzed with the same methods.
As a result, some chromone compounds including 2-(2-phenylethyl) chromone and
oxidoagarochromone derivatives, which are characteristic to agarwood were detected from artificial
agarwood on LC-MS. The amount and species of compounds were different among different treatments.
In present study, the constituents were compared among agarwood occurred in the same plant-
species in the same condition by different treatments, and they showed different species and amounts of
constituents. Previous studies often described generation process of resinous portions with a single
treatment and this study is different from them in the point that we compared between different
treatments. Our results may give leads to the improvement of quality evaluation and methods if treatment
for agarwood in future.
Keywords: artificial agarwood, chromone derivatives, Aquilaria sinensis

Fig. 1 Outline of experiments
References
[1] ANNUAL REPORT OF THE SECRETARIAT 2005-2006 Convention on International Trade in
Endangered Species of Wild Fauna and Flora
https://www.cites.org/sites/default/files/document/2005-06.pdf
[2] Yagura T, Shibayama N, Ito M, Kiuchi F, Honda G., Tetrahedron Letters, 46:4395-4398 (2005)
[3] Yang JL, Dong WH, Kong FD, Liao G, Wang J, Li W, Mei WL, Dai HF., Molecules 21: 911 (2016)
[4] Chen X, Zhu X, Feng M, Zhong Z, Zhou X, Chen X, Ye W, Zhang W, Gao X., Molecules
[5] 22,686 (2017)

P-I2
Design of Electronic Nose for Classification Agarwood of Gyrinops
versteegii
1*)
Farel Ahadyatulakbar Aditama, 1Rahadi Wirawan , 1Laili Mardiana , 1Tri Mulyaningsih , dan 1Nurul
Qomariyah
1
Faculty of Mathematics and Natural Sciences, Universitas Mataram,Lombok, NTB, Indonesia.
*) Correspondence author e-mail: farelfisika@outlook.com
Abstract
Agarwood is one of the natural products that has a high selling value. Aroma is one of the
parameters observed in determining the quality of agarwood and the selling value of agarwood. To be
able to measure the aroma of an agarwood requires an instrumentation system that is accurate, portable,
and economical. In this research an instrumentation system was built to classify agarwood (Gyrinops
versteegii), an electronic experiment system. This instrumentation system consists of a hardware system
with a resistive gas sensor consisting of TGS822, TGS2610 and TGS2620, a heating system and a
software system with MatLab 2015a and Arduino IDE that have used artificial neural network systems.
The hardware system works to read data from the results of agarwood installation and the software
system works to acquire data and carry out a quality classification process using an artificial neural
network system from reading the hardware system consisting of ADC value data.
The results show that the existing system in this electronic system can measure and clas sify
agarwood. Grynops versteegii which provides good quality in artificial neural networks [1 -1] and its
lower quality that is connected to artificial neural networks as [-11].
Keywords: Gyrinops versteegii, Electronic Nose System, Artificial Neural Networks.

P-I3
Antioxidant Activity of Agarwood Produced after Repeated Inoculation on
Gyrinops versteegii (Gilg.) Domke
Amalia Indah Prihantini*, Resti Wahyuni
Research and Development Institute of Non Timber Forest Product Technology, Jalan. Dharma Bhakti No.7,
Langko, Lingsar, Lombok Barat, NTB
Corresponding author e-mail: amaliaindah2@gmail.com
Abstract
Agarwood producing plant is a precious plants particularly in Asia. In Indonesia, the plants are
distributed in Borneo, Sumatera, Nusa Tenggara, Papua, Sulawesi, Java, and Moluccas. Several species
of the plants are grown in Lombok, West Nusa Tenggara i.e. Gyrinops versteegii, Gonystylus bancanus,
and G. macrophylus. G. versteegii is a most preferred endemic agarwood producing plant from Lombok.
The plant is wellknown for its resin production.The production of resin from agarwood tree is commonly
using inoculation methods. Several inoculation methods have been applied by the community. Likewise,
the research interest on G. versteegii mostly on its resin production. However, the species have not been
widely investigated as source of natural medicine, particularly antioxidant agent. The antioxidant
capacity of G. versteegii leaves have been reported, however, its resin produced after inoculation are
less studied. The present study was aimed to evaluate the antioxidant activity of agarwood produced
after repeated inoculation of G. versteegii.
The inoculation was conducted in repetition of every 0, 3, and 6 months. Furthermore, the agarwood
were harvested after 7 and 18 months inoculation. The samples were extracted in methanol and evaluated
their antioxidant activity using DPPH (1,1-diphenyl-2- picrylhydrazyl) radical scavenging assay at
concentration of 800 µg/ml. The result indicated that the more repetition will produce the lower
antioxidant activity. In addition, the results showed that generally a longer harvest time will produce
higher antioxidant activity. The highest antioxidant activity was shown by the sample after 18 months
inoculation (86.54±0.68%). The repetition of inoculation and the time of harvest give effects on
secondary metabolites production. As impact, it affect their antioxidant activity. The antagonistic and
synergetic interaction between secondary metabolites involved in the agarwood may contribute to the
antioxidant activity.
In general, repetition of inoculation might affect the production of secondary metabolites that have
responsible for the scent of agarwood. However, the agarwood produced without repetition of
inoculation is preferred. This is the first study reported the evaluation of antioxidant activity from
agarwood after repetition of inoculation. Furthermore, the result of the present study will contribute and
can be considered for the development of research on agarwood, particularly G. versteegii.
Keywords: Gyrinops versteegii, agarwood, inoculation, antioxidant.
Table 1. Antioxidant activity of agarwood after repeated inoculation at concentration of 800 µg/ml
Antioxidant activity (%)
Repetition of inoculation
After 18 months inoculation
After 7 months inoculation
68.34±2.31 86.54±0.68
Without repetition (P0)
69.41±0.87 75.99±5.21
Every 3 months (P1)
59.70±1.48 No activity
Every 6 months (P2)

References:
[1] Bahmani M, Golshahi H, Saki K, Kopaei MR, Delfan B, Mohammadi T, Asian Pacific journal of
Tropical Disease, 4: S687-S692, (2014).
[2] Hadi S, Muliasari H, Sukma NS, Ratnaningsih PEW, “Phytochemical screening and antibacterial
testing of gaharu trees (Gyrinops versteegii (gilg) domke) from Lombokisland” in Proceedings of
the 2nd International Seminar on Chemistry, Jatinangor, Indonesia, (2011).
[3] Mega IM and Swastini DA, Jurnal Kimia, 4(2): 187-192, (2010) [Indonesian].
[4] Nuringtyas TR, Isomaria R, Septia Y, Hidayati L, Wijayanti N, Moeljopawiro S, “The antioxidant
and cytotoxic activities of the chloroform extract of agarwood (Gyrinops versteegii (Gilg.) Domke)
leaves on HeLa cell lines” in Proceedings of the 5th International Conference on Biological
Science, Yogyakarta, Indonesia, (2018).
[5] Prihantini AI, Tachibana S, Itoh K, Pakistan Journal of Biological Science,17(10): 1106-1114,
(2014).
[6] Prihantini AI and Dewi KD, Asian Journal of Agriculture,3(1): 1-5, (2019).

P-I4
Study of Volatile Compound from The Nail Wounded Trees Producing
Agarwood (Gyrinops versteegii (Gilg.) Domke)
Ahmad Teguh Riadya, Ni Komang Tri Dharmayania, Ito Michihob, Isamu Yamadac, Surya Hadia*
aFaculty of Mathematics and Natural Science, University of Mataram, Lombok, NTB, Indonesia.
b Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
c Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan.
*) Corresponding author e-mail:sur_hadi88@yahoo.com
Abstract
The purpose of this research was to studythe volatile compounds of agarwood of
Gyrinopsversteegii (Gilg) Domkethat was produced from the nail-wounded trees. Nail- wounded-trees
is one of the method to wound the agarwood in order to produce agarwood.
The extraction was carried out using distillation and digestion techniques using methanol as a
solvent with different temperatures and time variations. Distillation used a temperature of 80 °C for 60
minutes while digestion was at 50 °C for 180 minutes. The crude extracts from both methods were
analyzed using Gas Chromatography Mass Spectrometry (GC-MS). The GC-MS was set to specific
program, which was: initial temperature of the oven was 40°C held for 10 minutes then increased to 260
°C for a total running time of 25 minutes.
The chromone, a volatile compound that is responsible for the aromatic fragrant of agarwood,
was identified only in the digestion extract. On the other side, the distillation method only detecting fatty
acids. The chromone is known to be namely 8-Methoxy-2-(2- Phenylethyl) Chromon-4-One with a
percent area of 1,75 % and retention time of 21,490. The volatil compound was found in GC-MS in the
range temperature of 40-260 °C.
Keywords: Eksploration, digestion, chromon, GC-MS
References:
[1] Pasaribu, G., Waluyo, T. K., & Pari, G. (2013). Analisis Komponen Kimia Beberapa Kualitas
Gaharu dengan Kromatografi Gas Spektrometri Massa. JurnalPenelitian Hasil Hutan, 31(3), 181-
185

P-I5
An Investigation of Volatile Compound from the Nail-Wounded- Trees
Producing Agarwood (Gyrinops versteegii) Based on GC-MS
Muhammad Rizki Juwardia, Murniatia, Ito Michihob, Isamu Yamadac, Surya Hadia*
aDepartment of Chemistry, Faculty of Mathematics and Science, University of Mataram,
bPharmaceutical Science, Kyoto University, Kyoto, Japan.
cCenter for Southeast Asian Study Kyoto University, Kyoto, Japan.
*Corresponding author E-mail: sur_hadi88@yahoo.com
Abstract
Nail-wounded-trees is one of the method of agarwood production by inserting a number of nails
in various parts of the gaharutrees. The purpose of this study was to investigate the common compounds
that are responsible for the aromatic fragrant of the agarwood from the wounded trees.
The agarwood sample was extracted using acetone, yielding the crude extract analyzed using Gas
Chromatography Mass Spectrometry (GC-MS) with various temperature programs. A chromone
compound was identified using GC-MS with parameter instrument: initial temperature 40 oC, held for
10 minutes then increased to 260 oC until the total running time 25 minutes. The chromone found to be
the 8-methoxy-2-(2-phenylethyl) chromen-4-onewith a percent area of 3.53% and a retention time of
21.396. A chromone compound, along with a terpene, is known to be a responsible compound for
aromatic fragrant of agarwood.
Keywords: investigation, chromone, GC-MS.
Table 1. Components of artificial agarwood (Gyrinops versteegii) chemical compounds

Molecular Compounds
Peak R. Time % Area Group
Formula
3.662 4.13 C6H10O Alkanes Isopropylideneacetone
1
5.089 24.23 C6H12O2 Alkanes Diacetone alcohol
2
8.774 3.20 C7H14O2 Alkanes 4-Methyl-4-methoxy-2- pentanone
3
8-methoxy-2-(2-
36 21.396 3.53 C18H16O3 Chromone phenylethyl)chromen-4-one
pentanal, 2-
[bis(phenylmethyl)amino]-4-
38 22.376 5.11 C20H25NO - methyl-
2-butylidenmalononitrile, 4- (p-
40 24.872 2.74 C14H14N2O - anisyl)-

Figure 1. Chromatogram of artificial agarwood (Gyrinopsversteegii), chromone marked by the arrow.
References:
[1] Pasaribu, G. T., Waluyo, T. K., dan Pari, G. 2015. Analysis of chemical compounds distinguisher
for agarwood qualities. Forestry Research, Development and Innovation Agency.

PHYTOCHEMISTRY MEDICINE USED,
PHARMACOLOGICAL ACTIVITY OF
AGARWOOD AND AROMATIC PLANT

P-P1
Toxicity Test of Agarwood (Gyrinops versteegii (Gilg.) Domke) Leaf
Methanol Extract Using Brine Shrimp Lethality Test (BSLT)
Jeksi Mahari Nurpratamaa, Emmy Yuanitaa, Ito Michihob, Isamu Yamadac, Surya Hadia*)
c Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan
*) Corespondence author e-mail: sur_hadi88@yahoo.com
Abstract
This study aims to determine the toxicity of agarwood (Gyrinops versteegii (Gilg.) Domke) leaf
methanol extract as an initial screening of anticancer agents. Toxicity tests were carried out using the
brine shrimp lethality test (BSLT) method then fractionated using vacum liquid chromatography (VLC)
with a mixture of solvents elution (n-hexane and ethyl acetate). The results showed that the crude
methanol extract was much more toxic than all the VLC’s fractionsas it showed in the Table 1. Thus, it
is indicating the possible synergism effect from the bioactive compoundsin the methanol extract. To
conclude, the methanol extract of agarwood (Gyrinops versteegii (Gilg.) Domke) leaf has a great
potential to be developed as an anticancer agent.
Keywords: Agarwood, Gyrinops versteegii, BSLT, anticancer, LC50
Tabel.1 LC50 value of methanol extract and fraction

LC50 (ppm)
Extract/Fraction
52.13
Methanol
493.09
F.1
687.62
F.2
493.09
F.3
173.43
F.4
173.43
F.5
493.09
F.6
References:
[1] Meyer, B., Ferrigni, N., Putnam, J., Jacobsen, L., Nichols, D., Mc Laughlin, J. Med. Plants
Res.,45:31–34, (1982).
[2] Nuringtyas, T.R., Isromarina, R., Septia, Y., and Hidayati, L., AIP conference proceedings,
020062:1-9, (2018).
[3] Khalil, A.S., Rahim A.A., Taha, K.K., Abdallah, K.B., Reasearch Publisher, 1(3):78-88, (2013).

P-P2
Toxicity Test of the Methanol Extracts of the Stem Bark of Agarwood
(Gyrinops versteegii (Gilg.) Domke) As an Anticancer Agents Using the
Brine Shrimp Lethality Test (BSLT)
Dian Lestaria, Ni Komang Tri Dharmayania, Ito Michihob, Isamu Yamadac, Surya Hadia*
*) Corresponding author e-mail: sur_hadi88@yahoo.com
Abstract
Gyrinops versteegii (Gilg.) Domkeis one of the agarwood species that endemically grow in
Lombok island. This plant has been utilized as a traditional medicine to treat kidney disorders,
painkillers, cancer, diarrhea, relieving stress, asthma, hepatitis and malaria. However, a bioactivity study
of this species has not been widely reported. Therefore, this research is aimed to study the bioactivity of
the stem bark of Gyrinops versteegii as a medicine, particularly for anti-cancer. The bark samples were
extracted using methanol for 3x24 hours, then the crude extract toxicity was tested using Brine Shrimp
Lethality Test (BSLT).
The test showed that the extract has LC50 values of 73.28 ppm. By the standards (toxic if
LC50<1000 ppm), the bark extract of agarwood (Gyrinops versteegii (Gilg.) Domke) has a great
potential as an anti-cancer agents.
Keywords: toxicity tests, BSLT, Gyrinops versteegii (Gilg.) Domke, LC50.
References:
[1] Meyer, BN, Ferrigni, NR, Putnam, JE, Jacobsen, LB, Nichols, DJ, & McLaughlin, JL Brine shrimp:
a convenient general bioassay for active plant constituents. Planta medica, 45 (05): 31-34, (1982).
[2] Wang, S., Yu, Z., Wang, C., Wu, C., Guo, P., & Wei, J. Chemical constituents and pharmacological
activity of agarwood and Aquilaria plants. Molecules, 23 (2): 342, (2018)

P-P3
Antioxidant Activity Test of Methanol Extract of Agarwood Leaves
(Gyrinops versteegii (Gilg.) Domke) Using DPPH Method
Fitra Fauhan Rahmasaria, Ni Komang Tri Dharmayania, Ito Michihob, Isamu Yamadac, Surya Hadia*
*) Corresponding author e-mail: sur_hadi88@yahoo.com
Abstract
Agarwood leaves (Gyrinopsversteegii (Gilg.) Domke) are expected to posses high antioxidant
activity as they reported to containphenol, flavonoid andtannin, an antioxidant agent. Antioxidant is an
important component in human’s body as it absorb and neutralize the free radical. Therefore, this
research aims to study the antioxidant activity from methanol extract of agarwood leaves
(Gyrinopsversteegii (Gilg.) Domke). The extraction method that used here was maceration, using
methanol as a solvent. The crude extract then tested for its antioxidant activity using the DPPH method
by measuring its absorbance at the maximum wavelength at 517 nm using UV-Vis spectrofotometer
with vitamin c (ascorbic acid) as the standard. The result of this test showed that the IC50 value of
methanol extract of agarwood leaves (Gyrinopsversteegii (Gilg.) Domke) is 45,74 ppm, which means it
have a high antioxidant activity.
Keywords: Agarwood leaves, Gyrinopsversteegii, Antioxidant, DPPH, IC50
Reference:
[1] Handayani, Selpida, Ahmad Najib, Nurul PurnamaWati. Jurnal Fitofarmaka Indonesia, 5(2): 299-
308 (2018).

P-P4
Rosmarinic Acid Comparison between Perilla Species
Yuya Deguchi, Michiho Ito
Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University Corresponding

author: michihoi@pharm.kyoto-u.ac.jp
Abstract
Shiso (Perilla frutescens Britton var. crispa W. Deane (Labiatae)) is an annual plant of Labiatae
and has unique fragrance. Its growing area spreads from India to East Asia and it is used as a flavored
and colored vegetable in Japan.The plant is also used as a traditional medicine; leaves of which both
sides are purple and with strong fragrance areconsidered as good product and are used for the purpose
of antitussive expectoration and sedation. Biosynthesis of its essential oil component is genetically
controlled and varieties of chemotypes can be found among them. In this study, we focused on
rosmarinic acid, an anti- inflammatory and anti-oxidant component, contained in perilla and other
Labiatae plants and compared its content in cultivated and wild species of perilla. Factors for different
content of rosmarinic acid were investigated especially for the genetic background by growing perilla
plants under controlled conditions. The relationship between rosmarinic acid and oil types was also
examined. Further investigations on rosmarinic acid in perilla will be discussed in the presentation.
・Flowchart of experiment
Keywords: Rosmarinic acid, Perilla plants, Essential oil
References
[1] Yamamoto H., Sakakibara J., et al. J. Agric Food Chem, 46, 862-865 (1998)
[2] Nakamura Y., Ohto Y., et al. J.Agric Food Chem, 46, 4545-4550 (1998)

TISSUE CULTURE OF EAGLEWOOD AND
AROMATIC PLANTS

P-T1
Methyl Jasmonate and Crude Extract of Fusarium solanielicit Agarwood
Compounds in Shoot Culture of Aquilaria malaccensis Lam.
Ahmad Faizala*, Rizkita Rachmi Esyantia, Nisaa Adn’aina, Silmi Rahmania, Alda Wydia Prihartini
Azara, and MamanTurjamanb
InstitutTeknologi Bandung, Jalan Ganeca 10, Bandung 40132, Indonesia

bForest Microbiology Research Group, R & D Centre for Forest, Environment & Forestry Research,
Development, and Innovation Agency (FORDA), Ministry of Environment and Forestry, Jalan
GunungBatu 5, Bogor, 16680, Indonesia
Corresponding author E-mail: afaizal@sith.itb.ac.id
Abstract
Agarwood is one of the commercially important commodities in the world market. However, its
production is limited due to lengthy time process, occurred very randomly, and decreasing number of
agarwood producing trees such as Aquilaria malaccensis in nature.
This research was established in in-vitro condition to evaluate the production of secondary
metabolites in Aquilaria malaccensis thin layer culture upon elicitation with methyl jasmonate (MeJA)
and crude extract of Fusarium solani strain Gorontalo (GSL1) or Jambi (GSL2). Elicitation was
performed by adding elicitors to A. malaccensis shoot culture grown in ½ MS liquid medium. Secondary
metabolite profile was analyzed by Gas Chromatography – Mass Spectrometry (GC-MS).
The results showed that chromone were detected after 3 weeks of co-culture time in the treatment
MeJA 12.5 and 25 μM. Styrene and D-Limonene which are compound of agarwood were detected in
the treatment GSL1 and GSL2. Thus, elicitation treatment induced higher production of secondary
metabolite compounds compared with control group with no treatment.
Keywords: Aquilaria malaccensis, elicitation, methyl jasmonate, Fusarium solani, secondary
metabolites
A B
Figure 1. Heatmap of secondary metabolite detected. [A]MeJA treatment, [B] Fungal treatment.

References:
[1] Ito, M., K. Okimoto, T. Yagura & G. Honda.,J. Essent. Oil Res.,17(2): 175-180, (2005).
[2] Jayaraman, S. & Mohamed, R., Turk. J. For., 39(2): 163-173, (2015).
[3] Jong, P.L., P. Tsan& R. Mohamed.,Int. J. Agric. Biol.,16(3): 644-648, (2014). 4. Naef, R.
FlavourFragr. J.,26: 73-89,(2011).
[4] Faizal, A. et al., Trees, 31: 189–197, (2017).

P-T2
Secondary Metabolite Profiles of Aquilaria malaccensis Lamk. Callus
Cultureelicited with Fusarium solani Mycelium Extract and Methyl
Jasmonate
Ahmad Faizala*, Rizkita Rachmi Esyantia, Nadia Puji Utamia, Maria Masitho Makajanmaa, and
Maman Turjamanb
InstitutTeknologi Bandung, Jalan Ganeca 10, Bandung 40132, Indonesia

bForest Microbiology Research Group, R & D Centre for Forest, Environment & Forestry Research,
Development, and Innovation Agency (FORDA), Ministry of Environment and Forestry, Jalan
GunungBatu 5, Bogor, 16680, Indonesia
Corresponding author E-mail: afaizal@sith.itb.ac.id
Abstract
Aquilaria malaccensisis a common agarwood-producing tree cultivated in Indonesia. The
agarwood formation can be induced by biological and chemical agents. Induced secondary metabolites
production in A. malaccensisin vitrocan be used as an alternative method to study the formation of
agarwood compounds. Thus, this study aimed to observe the effect of elicitation by F. solaniGSL1 and
GSL2 strains, and methyl jasmonate on the growth and secondary metabolite production in A.
malaccensiscallus culture. A. malacceniscalluswas induced on Murashige-Skoog (MS) medium with the
addition of 2 ppm 2,4-D (2,4-Dichlorophenoxyacetic acid) and 0,1 ppm (6-Benzylaminopurine) BAP.
Callus was subsequently subcultured to MS medium supplemented with 8 mg/L GSL1, GSL 2, and
MeJA 100 μM. Callus culture was incubated for 20 days in dark conditionatroom temperature. Callus
biomass was measured to determine growth rate and cell viability. Furthermore, callus was subjected to
GC-MS to determine the profile of secondary metabolites. The results showed that the treatments with
GSL1, GSL2, and MeJA did not significantly influence callus growth rate, but decreased cell viability
in the GSL1 (24%), GSL2 (17,1%), and MeJA (35%) treatments. GC-MS analysis showed that a typical
agarwood compound, chromone (6-methoxy-2- (4-methoxyphenetyl) -4H-chromen-4-on) was detected
in both GSL2 and MeJA-treated callus culture. It can be concluded that both fungal extract and
MeJAcould be employed to elicit the production of agarwood compounds in A. malaccensiscallus
culture.
Keywords: Aquilaria malaccensis, Elicitation, Agarwood, Callus, Chromone.

Figure 1. Relative cell viability.
Figure 2. Heatmap of secondary metabolite detected.
References:
[1] Dong, X., Gao, B., Feng, Y., Liu, X., Wang, J., Wang, J., and Shi, S.,Plant Cell, Tissue and Organ
Culture, 135: 53-62, (2018).
[2] Jayaraman, S. and Mohamed, R., Turk. J. For., 39(2): 163-173, (2015).
[3] Faizal, A. et al., Trees, 31: 189–197, (2017).
[4] Rasool, S., and Mohamed, R., Springer, Singapura. pp. 39-56, (.2016).
[5] Okudera, Y., and Ito, M, Plant Biotechnology, 26: 307-315, (2009).
[6] Walley, J. W., Kliebenstein, D. J., Bostock, R. M., andDehesh, K., Current Opinion in Plant
Biology, 16: 520-526, (2013).

PT-3
Distribution Mapping of Agarwood Plantation in Lombok Island
Nurul Fitriyani1)*, Muhammad Sopian Sauri2), Mamika Ujianita Romdhini2), Agus Kurnia2), Surya
Hadi2), Tri Mulyaningsih2), Michiho Ito3), Isamu Yamada4)
1)*Faculty of Mathematics and Natural Sciences, UniversitasMataram, Jalan Majapahit 62, Mataram, 83125,
Indonesia, nurul.fitriyani@unram.ac.id
2)Faculty of Mathematics and Natural Sciences, UniversitasMataram, Jalan Majapahit 62, Mataram, 83125,
Indonesia
3)Graduate School of Pharmaceutical Sciences, Kyoto University, 46–29 Yoshida Shimoadachi-cho, Sakyo-ku,
Kyoto 606–8501, Japan
4)Center for Southeast Asian Studies, Kyoto University; 46 Shimoadachi-cho, Yoshida Sakyo- ku, Kyoto606-
8501, Japan
Abstract
In this study, researchers describe the distribution of Agarwood plantation. This research aimed
to map the distribution of Agarwood farming in several locations on Lombok Island. A survey was
conducted through interviews and questionnaires, with selected respondents from Agarwood farmers of
Agarwood. In addition, to obtain data about the agarwood plants, researchers also conduct field research
at the location of agarwood plantations. The study was conducted by using Geographic Information
System (GIS) to map the spatial distribution of Agarwood plantation. This study is expected to be useful
for related parties to be able to find out the distribution of agarwood plantation in Lombok Island.
Keywords: Field Research; Geographic Information System; Gyrinops; Statistical Analysis; Survey.
References
[1] Arbia, G., 2006, Spatial Econometrics: Statistical Foundations and Applications to Regional
Convergence, Germany: Springer-Verlag Berlin Heidelberg.
[2] Gaetan, C. and X. Guyon, 2010, Spatial Statistics and Modeling, Translated by Kevin Bleakley,
New York: Springer Science Business Media.
[3] Mulyaningsih, T. and I. Yamada, 2008, Notes on Some Species of Agarwood in Nusa Tenggara,
Celebes, and West Papua, Natural Resource Management and Socio-Economic Transformation
Under the Decentralization in Indonesia, Towards Sulawesi Area Studies, CSEAS, Kyoto.
[4] Mulyaningsih, T., A. Muspiah, E. Arianty., S. Hadi., N. Fitriyani., M. Ito., and I. Yamada, 2018,
Variasi Keragaman Morfologi Pohon Gaharu di NTB, Indonesia. Paper of 1st International
Conference and Workshop in Bioscience dan Biotechnology by Bioscience dan Biotechnology
Research Center University of Mataram, November 28th – 30th, 2018, Mataram.
[5] Mulyaningsih, T., D. Marsono, and I. Yamada, 2015, Community of Eaglewood Gyrinops
versteegii (Gilg.) Domke and The Diversity of Plant Species Associated in Western Lombok Forest,
Proceeding of ICST, pp.361 – 382.
[6] Mulyaningsih, T., D. Marsono, I. Yamada, 2016, Community of Eaglewood Gyrinops versteegii
(Gilg.) Domke and The Diversity of Plant Species Associated in Western Lombok Forest, ICST
Proceeding, pp. 361- 382.

MISCELLANEOUS

GETTING AROUND IN MATARAM
Lombok Internasional Air (LIA), located near the city of Praya, Lombok Tengah is
approximately 40 km south of Mataram.
1. Airport shuttle bus
DAMRI a public bus service is available from Lombok Internasional Airportto serve passengers
who are wishing to travel to and from either Mataram or Senggigi. The 8 DAMRI buses serving
the route have a capacity of approximately 40 passengers and 10 buses with a 20 passengers.
They are new air-conditioned buses and are white in colour.
DAMRI services depart from their office located in eastern Mataram on Jl. Tuan Guru Faisal
(a three minute taxi ride from the Mandalika Bus Station) every hour and proceed to the airport,
commencing at 04:00 with the last bus leaving that terminal at 19:00.
DAMRI services provided to connect Senggigi to the airport, departing Senggigi from 03:00
and then every 1.5 hr until the last departure from Senggigi at 20:00. Some airport buses may
provide services between Mandalika and Senggigi. In the last couple of months these services
have not been accurately following the published schedules, ensure you leave plenty of buffer
time if catching a departing flight.
The bus fare from/to Mataram is IDR 35,000 and from/to Senggigi is IDR 45.000 Taxis are
normally available at the terminal for travel onward to other areas.
2. By boat
The port of Lembar is 22 km south of Mataram. There are ferries every 90 minutes or so, 24
hours a day, from Padang Bai on Bali, with the trip taking 4-5 hours. Any volunteer "porters"
who carry your bags will expect at least Rp. 10,000.
If you haven't booked an end-to-end travel package, getting from Lembar to Mataram can
sometimes be problematic. You can take a shuttle bus service to Mataram with Perama Tours
and Travel, Pejanggik Str. No. 66, Mataram, ☎+62 370 635928. Perama have offices across
Indonesia including Bali and Lombok providing land sea and air connections. Perama shuttle
bus offers Bali to Mataram connections and Lembar to Mataram connections. An hotel pick-up
should always be arranged well in advanced and preferably confirmed by email. An alternative
is to negotiate a trip with a private car operator upon arrival. You can also take an online
transportation by using an apps. In your mobile phone (GO-JEK or GRAB).Alternatively,
metered taxis are often available at the terminal or just outside the gates. A taxi may be available
but do not depend on one being there. There are a few basic warung and losmen in Lembar if
you get stuck.

1.1 Get Around Mataram City
1.1.1 By taxi
Taxi fares are quite reasonable if they use the meter. Some drivers may try and enforce IDR
25,000 minimum. Blue bird Lombok Taxi, ☎+62 370 627000is the most common taxi available
in Mataram. Both the Bluebird and white coloured Express Taksi have a radio despatch system
and both companies cars are equiped with meters which should be used by their drivers at all
times. Flag fall is approximately Rp 6,000 and the meter ticks up a few hundred rupiah for every
hundred meters past 2 km. Taxis can be booked in advance either by calling them yourself or
by booking through your hotel. Taxis may be hailed down on the street anywhere around
Mataram with the exception of the dark blue Airport Taxi the use of which is limited to arriving
airport passengers only.
1.1.2 By GO-JEK or GRAB
GO-JEK and GRAB are online transpotation that can be reach by using an apps in your mobile
phone.You can also take an online transportation by using an apps. In your mobile phone (GO-
JEK or GRAB). Alternatively, metered taxis are often available at the terminal or just outside
the gates.
1.1.3 By ojek
Prices are negotiable but a rule of thumb is Rp 5,000-10,000 for a short trip with a local
motorbike rider if the destination is nearby, longer distances will require some informed
negotiation.
1.1.4 By horsecart (Cidomo)
Horse carts known as cidomo remain a common means of transport in Mataram and they are
often found operating throughout the congested streets near a pasar or produce market. Ojek
and metered taxis are more commonly used for transport in and around Mataram.
1.1.5 By car
Renting a car is a possible alternative in Mataram. It will cost around IDR 300,000 for a Toyota
Avanza/Daihatsu Xenia (2x4) 6-8 seats, IDR 450,000 for a Toyota Kijang (2x4) with 7-8 seats.
Age and condition of car will effect price as will high rental demand in the peak tourism period
around the June-August and local holidays. Rental periods should be for 24hours.
1.1.5 By motorbike
Motorbikes are also available for rent around Mataram. Expect to pay approximately
IDR70,000 per day for a motor bike in as new condition. There are several rental operators to
be found in the Cakranegara area or enquire at your hotel for assistance.

• Lombok Motor Bike Motorbike Rental Kota Mataram– Lombok, rate starts from IDR70.000
per 24 hours. Motorbike will be delivered and pick up at hotel near Mataram or Damri Bus
terminal. Booking can be made via booking@lombokmotorbike.com.
• Don't miss Lombok's beautiful scenery. If you choose to rent a car or motorcycles and drive
yourself, you must have an International Driving License.
• Renting a car or motorbike are good alternative to get around Lombok, either self driving or
with driver so you can relax and enjoy the view. (Tip driver pocket money for meals if you stop
for lunch or dinner). If you are pleased with service, tip (IDR. 20,000 minimum). If you collide
with anything, or it collides with you, you are responsible for all cost.

BANKING INFORMATION
Money & Local Currencies
When changing money there are many money changers in Lombok. The best rates are usually
in Senggigi. Money changers prefer new, clean large bills and will accept smaller
denominations at a lower rate.
If you have traveler’s checks the rate will be less than bank notes. Be sure to count your money
before you leave money changers. You can also check local banks but the rate is slightly lower
than outside. Re-count the money in front of the money changer reception is a must, as usually
there's a quick tricky way how they can steal your money.
A suggestion when changing money:
If you plan on making small purchases be sure to carry small denominations with you because
many sellers do not have change. The Rupiah come in denominations of 100,000; 50,000;
20,000; 10,000; 5,000;2,000; 1,000 and 1,000; 500; 200;100 in coin.
Business Hours
Indonesian work in the morning to avoid the heat of the day. If you need to visit a government
office, arrive between 08 AM and 11:30 AM. Government offices close early on Fridays.
Generally offices are open 07:30AM - 3 PM Monday - Thursday, 07:00AM - noon on Friday.
Banks and Government Offices are closed on Saturday and Sunday.
Money Changer:
1. Tokoemasmelati& money changer: Pejanggik No.6 Pertokoan Cakra PlazaMataram, Lombok
83231, Indonesia, Phone: +62 370 631567 Email: @tokoemasmelati.
2. Mandiri Bank: at Jalan Sri Wijaya, at Jalan Erlangga.
3. BNI Bank: Jalan Erlangga; and Jalan Langko.
4. BCA: at Cakra Negara.
5. BMC money changer at Senggigi.
ATM
1. ATM (Mandiri Bank, BNI Bank, BPD bank, BRI Bank) left and right side at Jalan SriWijayain
front of Post office.
2. ATM (Mandiri Bank, BNI Bank, BCA bank, BRI Bank) at Jalan Sriwijaya, at Epicentrum Mall
Jalan Sriwijaya.
3. ATM (Mandiri Bank, BNI Bank, BCA bank, BRI Bank) at Jalan Erlangga.

MATARAM CITY MAP

LIST OF PARTICIPANS

Name Institute E-mail addres
Agus Sukito Research and Development sukitokun@gmail.com

Intitute of Non-Timber Forest
Product Technology, Ministry of
Environment and Forestry Jl.
Dharma Bakti No.7, Lingsar,
Lombok Barat District, Nusa
Tenggara Barat Province –
Indonesia.
Ahmad Faizal Plant Science and Biotechnology afaizal@sith.itb.ac.id

Research Group, School of Life
Sciences and Technology,
InstitutTeknologi Bandung,
Ganesa 10 Bandung 40132,
Indonesia.
Ahmad Teguh Faculty Mathematics and sur_hadi88@yahoo,com

Riyadi Natural Science, University of
Mataram, Lombok, NTB,
Indonesia
Alda Widya Plant Science and Biotechnology Afaizal@sith.itb.ac.id

Prihartini Azara Research Group, School of Life
Science and Technology, Institut
Teknologi Bandung, Indonesia
Amalia Indah Research and Development amaliaindah2@gmail.com

Prihantini Institute of Non Timber Forest
Product Technology, Jalan.
Dharma Bhakti No.7, Langko,
Lingsar, Lombok Barat, NTB,
Indonesia.
Baiq Erna Listiana Faculty of Agriculture, Mataram e.listiana@unram.ac.id

University, Jl.
Majapahit No. 62, Mataram
83127, Indonesia.
Dian Lestari Faculty Mathematics and sur_hadi88@yahoo.com

Natural Science, University of
Indonesia
Erna Listiana Faculty of Agriculture, e.listiana@unram.ac.id

Mataram University, Indonesia

Ernin Hidayati Departement of Biology, Faculty hidayatiernin@unram.ac.id
Mathematics and Natural
Science, University of Mataram,
Indonesia
Farel Ahadyatul Faculty Mathematics and farelfisika@outlook.com
Akbar Aditama Natural Science, University of
Indonesia
Fitra Fauhan Faculty Mathematics and Sur_hadi88@yahoo.com
Rahmasari Natural Science, University of
Indonesia
Hussain Ahmed Doctor retired, previously dr.hussain_uea@hotmail
Hussain Alrefaei worked in the Department of
Education and Knowledge In
U.A.E; United Arab Emirated
Isamu YAMADA CSEAS Kyoto Universitas yamada@cseas.kyoto-u.ac.jp
Jeksi Mahari Faculty Mathematics and Sur_hadi88@yahoo.com

Nurpratama Natural Science, University
of Mataram, Lombok, NTB,
Indonesia
Karmilawati Departement of Biology, trimulya@unram.ac.id
Faculty Mathematics and
Natural Science, University
of Mataram, Indonesia
Kurniasih Biology Departement, Faculty kurniasihs@unram.ac.id
Sukenti Mathematics and Natural
Science, Mataram University,
Indonesia
Laily Hunawatun Departement of Biology, trimulya@unram.ac.id
Sani Faculty Mathematics and
Mataram, Indonesia
Lalu Arya Biology Study Program, yzamroni@unram.ac.id
Kasmara Faculty Mathematics and
Natural Science, Universitas
Mataram, Indonesia
Lina Karlinasari Forestry Faculty, IPB, Bogor, Karlinasari@apps.ipb.ac.id
Indonesia
Lukmanul Departement of Biology, trimulya@unram.ac.id
Hakim Faculty Mathematics and
Mataram, Indonesia
MamanTurjaman Pusat Penelitian dan turjaman@gmail.com
PengembanganKonservasi dan
RehabilitasiHutan, KLHK.
In Bogor.

Marlina Ardiani Herbarium Bogoriense marlina.adriani@gmail.com
Botany Devision Research
Centre of Biology, Indonesia
Instsitute of Science (LIPI),
Indonesia
Michiho ITO The Graduate School of michihoi@pharm.kyoto-u.ac.jp
Pharmaceutical Sciences,
Kyoto University
Muhammad Alif Department of Basic Medical muhdalifmazlan@gmail.com
Bin Mazlan Sciences, Kulliyyah of
Nursing, International Islamic
University of Malaysia,
Kuantan Campus, Pahang,
Malaysia
Muhammad Department of Basic Medical lokman@iium.edu.my
Lokman Md Isa Sciences, Kulliyyah of
Nursing, International Islamic
Malaysia
Muhammad Departement of Chemistry, Sur_hadi88@yahoo.com
Rizki Juwardi Faculty Mathematics and
Science, University of
Mataram
Najihah Mohd International Instiute for Halal najihahmnoor@gmail.com
Noor Research and Training
(INHART) International
Islamic University Malaysia
(IIUM), Kuala Lumpur,
Malaysia
Nur Aimi Aliah Departement of Biotechnology yumi@iium.edu. My
Zainurin and Engineering, Kulliyyah of
Engineering, International
(IIUM), Kuala Lumpur,
Malaysia
Nurhusna International Instiute for Halal nurhusna@iium.edu.my

Samsudin Research and Training
International Islamic
University Malaysia (IIUM),
Gombak Selangor
Nurul Fitriyani Faculty Mathematics and nurul.fitriyani@unram.ac.id
Science, University of
Mataram, Mataram, Indonesia
Nurul Wahyuni NTFP Technology Reasearch Nuni.colin@gmail.com

and Depelopment Institute

Redzuan Nul Department of Basic Medical redzuan@iium.edu.my
Hakim Abdul Sciences, Kulliyyah of
Razak Nursing, International Islamic
Malaysia
RestiWahyuni Non-Timber Forest Product resti_bio@yahoo.com
Technology Research and
Development Institute, Jalan
Dharma Bhakti No.7, Ds
Langko, Lingsar, Lombok
Barat, NTB.
Robert A. Department of Plant Pathology,
Blanchette 1991 Upper Buford Circle, 495
Borlaug Hall; University of
Minnesota, St. Paul,
Minnesota 55108-6030 U.S.A.
Rubangi Al Non - Timber Forest Product rubhasan@yahoo.com

Hasan Technology Research and
Development Institute. Jl.
Dharma Bhakti No. 07,
DesaLangko, Kec.
LingsarKab. Lombok Barat,
West Nusa Tenggara.
Rubangi Al- Centre for Forestry and upuls@sjp.ac.lk
hasan Environment, Departement of
Forestry and Environment,
University of Sri
Jayewardenepura, Nugegoda,
CO 10250, Srilangka
S.M.C.U.P. Department of Forestry and
Subasinghe Environmental Science,
University of Sri
Jayewardenepura, Sri Lanka
Sakura Graduate School and Faculty michihoi@pharm.kyoto-u.ac.jp
Takamatsu of Pharmaceutical Sciences,
Kyoto University. 46-29
Yoshidashimoadachi-cho,
Sakyo-ku, Kyoto 606-8501,
Japan
Seiji Tanaka Graduate School of michihoi@pharm.kyoto‑u.ac.jp
Pharmaceutical Sciences,
KyotoUniversity. 46-29
Yoshidashimoadachi-cho,
Japan.

Supriyanto SEAMEO BIOTROP, BOGOR supriyanto@biotrop.org
Department of Silviculture,
Faculty of Forestry, IPB-
University
Surya Hadi Mathematics and Science Sur_hadi88@yahoo.com
Faculty, University Of
Mataram, West Nusa
Tenggara, Mataram,
Indonesia
Thifali Adzani, Division of Forestry and thif.adzani@gmail.com
Biomaterials Science, Graduate
School of Agriculture, Kyoto
University
KitashirakawaOiwake-cho,
Japan.
Tri Mulyaningsih Faculty of Mathematics and trimulya@unram.ac.id

Natural Sciences, Mataram
University, Jl. Majapahit No.
62, Mataram 83127,
Indonesia.
Via Febrianti Plant Biodiversity Club, trimulya@unram.ac.id
Faculty of Mathematics and
University, Indonesia
Vira heryanto Plant Biodiversity Club, trimulya@unram.ac.id
Wiratno, M.Sc. Dirjen. KSDAE
KementrianLingkunganHidup
dan Kehutanan
In Jakarta
Wiwin Hidayat Plant Biodiversity Club, trimulya@unram.ac.id
Yuliadi Zamroni Biology Study Program, yzamroni@unram.ac.id
Faculty Mathematics and
Mataram, Indonesia
Yulizah Research Center For Biology, yulizah.rhiezha@gmail.com
Indonesian Institute of Science.
Cibinong Science Center, Jl.
Raya Jakarta Bogor KM 46,
Cibinong, West Java 16911.

Yumi Zuhanis International Institute for Halal yumi@iium.edu.my
Has-Yun Hashim Research and Training
(INHART), International
(IIUM), Jalan Gombak, 53100
Kuala Lumpur, Malaysia.
Yuya Deguchi Department of michihoi@pharm.kyoto-u.ac.jp

Pharmacognosy, Graduate
School of Pharmaceutical
Sciences, Kyoto University.
Zainul Muttaqin Biology Study Program, trimulya@unram.ac.id
Sany Faculty Mathematics and
Mataram, Indonesia

GENERAL
INFORMATION

TOPICS
SCOPE OF PRESENTATION
1. Commerce, policies and regulations of agarwood.
2. Ecology, environment, conservation, molecular, cytogenetic, anatomy and biosystematics of
agarwood and aromatic plants.
3. Agarwood induction and biosynthesis, products development, extraction technologies,
chemical constituents and grading technology, botany, physiology, its symbiont and pathology
of agarwood and aromatic plants.
4. Secondary use, traditional medicine, pharmacological activity of agarwood and its cell target in
the body, ethnobotany of agarwood and aromatic plants.
5. Tissue culture, domestication, management of wild and planted agarwood taxa and planting
technology of eaglewood and aromatic plants.

EVENT, VENUE, AND HOTEL AROUND
VENUE
Schedule
Symposium (October 1, 2019) : 08.00 – 17.00 local time.
Welcome Dinner (October 1, 2019) : 19.00 – 21.00 local time
(only for registered welcome dinner participant)
Fullday Excurtion (October 2, 2019) : Trip, 07.00 – 18.15 local time
Dinner, 19.00 – 20.30 local time
(Primarasa Resto in Gegutu, Mataram)
Venue
The 1st ISAAP 2019 will be held in Golden Palace Hotel, Jl. Sriwijaya No. 38 Mataram,
Lombok - Indonesia.
Conference Room: Garuda Ballroom I, Garuda Ballroom II, and Camar Room

Venue

Venue

EXCURTION GUIDE TOUR
Time Schedule
Wednesday, October 2, 2019
Route:
07.00 – 07.30 → Registration and gathering at Golden Palace Hotel
07.30 – 10.30 → Trip to Labuan Pandan Beach, Eas Lombok.
10.30 – 10.45 → Trip to Gili Petagan (Gili Lampu)
10.45 – 12.15 → Tour to Gili Lampu
12.00 – 12.15 → Back to Labuan Pandan Beach.
12.10 – 13.00 → Lunch and Prayer Time in Labuan Pandan Beach
13.00 – 13.20 → Trip to Big Ancient Tree Ficus albipila (Miq) King
13.20 – 13.50 → Tour to Big Ancient Tree Ficus albipila (Miq) King
13.50 – 17.00 → Trip to Agroforestry of Natural Gyrinops versteegii,
Pemepek, Central Lombok.
17.00 – 17.30 → Tour to Agroforestry of Natural Gyrinops versteegii,
Pemepek, Central Lombok.
17.30 – 18.15 → Trip to Prima Rasa Resto
18.15 – 19.00 → Prayer Time
19.00 – 20.30 → Dinner and Art Performance at Prima Rasa Resto.

Excursion Map

✓ Gili Lampu, Sambelia, East Lombok.

✓ Labuhan Pandan Beach, East Lombok.
Lunch and prayer time in Labuhan Pandan Beach, East Lombok.

✓ BigAncient tree, Lian (Ficus albipila (Miq.) King) at Jalan Raya Sambelia, Gunung Malang,
Pringgabaya, East Lombok.

✓ Agroforestry of Natural Gyrinops versteegii, Pemepek Central Lombok.
Natural Gyrinops versteegii grew among other trees, such as: Arenga pinnata, Cocos nucifera,
Gnetum gnemon, Durio zibethinus, bamboo (Gigantocloa atter), etc.

✓ Prima Rasa Resto
Dinner in Primarasa Resto

AUTHOR INDEX

Name Page
Agung Adi Nugroho 71
Agus Kurnia 114
Agus Sukito 69
Ahmad Faizal 55, 110, 112
Ahmad Jupri 91
Ahmad Teguh Riady 101
Aida Muspiah 84, 87
Ainun Astrid 90
Alda Wydia Prihartini Azar 54, 110
Amalia Indah Prihantini 52, 78, 99
Asep Hidayat 37
Asmak Muti’ah Mohd Nasir 34, 48
Azantee Yazmie Abdul Wahab 42
Baiq Desy Ratnasari 43
Baiq Farista 91
Cecep Handoko 69
Dian Lestari 106
Emmy Yuanita 105
Erna Listiana 68
Ernin Hidayati 65
Farel Ahadyatulakbar Aditama 98
Faridah Ismail 42
Faturrahman 65
Ferdinand Schroeder 68
Fitra Fauhan Rahmasari 107
Hashim, Y. Z, H-Y. 49
Hitihamu, I., 30
Hussain Ahmed Hussain Alrefaei 58
Hussin Muhammad 42, 46
I Gede Nano Septian 91
I Wayan Suana 93
Immy Suci Rohyani 94
33, 38, 43, 64, 87, 91, 94, 101, 102,
Isamu Yamada
105, 106, 107, 114
Ito Michiho 43, 64, 101
Janwar Wahyu 89
Jeksi Mahari Nurpratama 105
Joeni Setijo Rahajoe 71
Karmilawati 84
Koji Matsushita 73
Kurniasih Sukenti 43, 94

Name Page
Kusumadewi S. Yulita 74
Laili Mardiana 98
Laily Hunawatun Sani 89
Lalu Arya Kasmara 93
Lina Karlinasari 32
Lina S. Juswara 74
Lukmanul Hakim 86
Lulut D. Sulistyaningsih 74
Lutfi Anggadhania L. 57
Malithi, R.A.P., 30
Maman Turjaman 36, 55, 110, 112
Mamika Ujianita Romdhini 114
Mamoru Kanzaki 73
Mansyur 78
Maria Masitho Makajanma 112
Marlina Ardiyani 74
29, 38, 43, 64, 81, 84, 87, 91, 94, 96,
Michiho Ito
101, 105, 106, 107, 108, 114
Muhammad Alif Mazlan 42
Muhammad Lokman Md Isa 42, 46
Muhammad Rizki Juwardi 102
Muhammad Sopian Suauri 114
Murniati 102
Najihah Mohd Noor 48
Natasha Jafar Ali 44
Nazurah Ab llaha 34
Ni Komang Tri Dharmayani 101, 106, 107
Ni Nengah Sri Eni 86
Nilam Fadmaulidha Wulandari 64
Nisaa And'Aina 110
Nur Aimi Aliah Zainurin 44
Nur Umisha Zainudin 48, 50
Nurhusna Samsudin 34, 50
Nurul Fitriyani 114
Nurul Qomariyah 98
Nurul Wahyuni 57
Puji Utami 112
Rahadi Wirawan 98
Raja Arif Shah Raja Ismail 42
Rani Asmarayani 74
Redzuan Nul Hakim Abdul Razak 42, 46

Name Page
Resti Wahyuni 53, 99
Rizkita Rachmi Esyanita 110, 112
Rubangi Al Hasan 78
S.M.C.U.P. Subasinghe 30
Sakura Takamatsu 96
Sarkono 64
Seiji Tanaka 81
Seni K. Senjaya 74
Silmi Rahmania 110
Sudirman 63
Sukiman 94
Sumarjan 68
43, 91, 94, 101, 102, 105, 106, 107,
Surya Hadi
114
Susila 74
Thifali Adzani 73
38, 43, 64, 68, 83, 84, 86, 87, 89, 90,
Tri Mulyaningsih
91, 94, 98, 114
Ulrich Schurr 68
Ummu Athiyah Mohammad Tormizi 34, 48, 50
Via Febrianti 90
Vidurangi, A.N.K.G 30
Wita Wardani 74
Withanage, S., 30
Wiwin Hidayat 83
YMM Anita Nugraheni 57
Yuliadi Zamroni 43, 91, 93
Yulizah 71
Yumi Zuhanis Has-Yun Hashim 34, 44, 48
Yuya Deguchi 108
Zainul Muttaqin Sany 87

st
1 ISAAP 2019
Secretarial
1st International Symposium of Agarwood and Aromatic Plants 2019
Faculty of Mathematics and Natural Sciences, Mataram University

Jalan Majapahit No 62, Mataram Lombok 83125 Indonesia.
E-mail: isaapp@unram.ac.id
Website:http://www.isaapp2019.unram.ac.id
Phone: +62 - 370 - 646506
CONTACT PERSON
Dr. Tri Mulyaningsih, M. Si.

trimulya@unram.ac.id
Dr. Kurniasih Sukenti
kurniasihs@unram.ac.id
Nurul Fitriyani, M. Si.

nurul.fitriyani@unram.ac.id


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PROGRAMME BOOK

1st INTERNATIONAL SYMPOSIUM ON AGARWOOD

Lombok, October 1 – 2, 2019

Faculty of Mathematics and Natural Sciences Mataram University,

We cordially invite you to attend and participate in this significant event.

Dr. Tri Mulyaningsih, M. Si.

1st International Symposium on Agarwood and Aromatic Plants 2019

1st International Symposium on Agarwood and Aromatic Plants 2019

Mathematics and Natural Sciences, Mataram University).

Prof. Emrt. Isamu Yamada (CSEAS Kyoto University, Japan)

Ir. H. Rosiady Husaenie Sayuti, M.Sc., Ph.D. (Mataram University)

1st International Symposium on Agarwood and Aromatic Plants 2019

Treasurer : Mamika Ujianita Romdhini, M.Si.

Fundraising : Dr. Ernin Hidayati

Secretarial : Dr. Yuliadi Zamroni

Program : Dr. Bambang Fajar Suryadi

Poster : Suripto, M.Si.

Consumption : Aida Muspiah, M.Si.

Excursion : Dr. Sarkono Sukiman, MSi.

1st International Symposium on Agarwood and Aromatic Plants 2019

1st International Symposium on Agarwood and Aromatic Plants 2019

08.00 – 09.00 Registration

09.00 – 09.40 Opening Ceremony

09.00 – 09.10 Delivering Indonesian National Anthem (“Indonesia Raya”)

09.10 – 09.20 Committee Report

09.20 – 09.30 Welcome Speech (Rector of Mataram University)

09.30 – 09.40 Welcome Speech and Opening Ceremony

(Governor of West Nusa Tenggara)

09.40 – 09.55 Coffee Break

09.55 – 12.30 Plenary Session I (Keynote Lectures)

Moderator: Prof. I Made Sudarma

09.55 – 10.20 Prof. Michiho ITO

10.20 – 10.45 Prof. S.M.C.U.P. Subasinghe

10.45 – 11.10 Prof. Lina Karlinasari

11.10 – 11.35 Prof. Emeritus Isamu YAMADA

11.35 – 12.00 Dr. Maman Turjaman

12.00 – 12.30 Discussion

12.30 – 13.30 Prayer Time, Lunch Break, and Poster Session

13.30 – 15.15 Plenary Session II (Keynote Lectures)

Moderator: Prof. Surya Hadi

13.30 – 13.55 Assoc. Prof. Yumi Zuhanis Has-Yun Hashim

13.55 – 14.20 Dr. Dadang Wardana

1st International Symposium on Agarwood and Aromatic Plants 2019

14.45 – 15.15 Discussion

15.15 – 15.30 Coffee Break

15.30 – 16.50 Parallel Session

15.30 – 16.50 Garuda Ballroom I (scope)

Moderator: Eka Sunarwidhi Prasedya, Ph.D.

Secondary Use, Phytochemistry, Traditional Medical, Pharmacological

15.30 – 16.50 Garuda Ballroom II (scope)

Moderator: Dr. Irwansyah

Induction of Agarwood, Commerce, Chemical Constituents of Agarwood

15.30 – 16.50 Camar Meeting room (scope),

Moderator: Hj. Suryani Eka W., ST., MBA., Ph.D.

Tissue Culture, Ecology, Biosistematic, Management of Wild and Planting

16.50 – 17.00 Closing ceremony

19.00 – 21.00 Welcome Dinner (for registered participant only)

1st International Symposium on Agarwood and Aromatic Plants 2019

1st International Symposium on Agarwood and Aromatic Plants 2019

CODE TITLES AUTHOR

1st International Symposium on Agarwood and Aromatic Plants 2019

1st International Symposium on Agarwood and Aromatic Plants 2019

(Rector of Mataram University)