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4600 SERIES

CHEMISTRY ANALYZER

Service Manual

©2009 Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual
is restricted for use by factory authorized personnel and is to be used for the sole purpose of providing routine
instrument maintenance and repair service. No part of this manual may be copied or redistributed without the
express consent of Awareness Technology, Inc. There is not transfer of technology, copyright, trade name, patent,
trade secret, or other proprietary right given or implied. Doc. 4600 Rev. A 11/2011
TABLE OF CONTENTS

1. INTRODUCTION .......................................................................................................1
1.1 ChemWell-T........................................................................................................................................................1

1.2 Warnings and Precautions.................................................................................................................................2


1.2.1 Intended Use .................................................................................................................................................2
1.2.2 General Safety Summary ..............................................................................................................................3
1.2.3 To Avoid Fire or Personal Injury..................................................................................................................3
1.2.4 Safety Terms and Symbols ...........................................................................................................................4

1.3 Layout..................................................................................................................................................................5
1.3.1 Instrument Parts and Controls.......................................................................................................................7

1.4 Technical Specifications ...................................................................................................................................28

2. PRINCIPLES OF OPERATION...............................................................................30
2.1 Electronic Hardware Description ...................................................................................................................30
2.1.1 Master Controller (eZ80) PCB....................................................................................................................30
2.1.2 Motor Controller PCB ................................................................................................................................33
2.1.3 Photometer PCB .........................................................................................................................................36
2.1.4 Diluter PCB ................................................................................................................................................38
2.1.5 PCB Light Diagnostic Guide ......................................................................................................................39

2.2 System Communication Description...............................................................................................................46


2.2.1 System Protocol Description ......................................................................................................................46
2.2.2 Slave Programming ....................................................................................................................................46

2.3 Power Subsystem ..............................................................................................................................................47

2.4 Computer to ChemWell-T Communication...................................................................................................53


2.4.1 Firmware versus Software ..........................................................................................................................53

2.5 Motion Control .................................................................................................................................................53


2.5.1 Mixer ..........................................................................................................................................................53
2.5.2 Probe X .......................................................................................................................................................53
2.5.3 Probe Z .......................................................................................................................................................54

2.6 Syringe Pump....................................................................................................................................................55

2.7 Photometer ........................................................................................................................................................55

2.8 Computer Connections ....................................................................................................................................56

2.9 Computer Software Interface..........................................................................................................................56

2.10 Instrument Routines.......................................................................................................................................56


3. SERVICE PROCEDURES ......................................................................................57
3.1 Enclosure...........................................................................................................................................................57
3.1.1 Enclosure Removal Procedures ..................................................................................................................58
3.1.2 Removal of Cuvette Cover Assembly (part #994626SP) and Syringe Diluter Cover (part #189637SP) ...58
3.1.3 Removal and Re-installation of Top (needed to remove the Shield) ..........................................................58
3.1.4 Removal and Re-installation of the Shield Assembly (part #994625SP)....................................................59
3.1.5 Removal and Re-installation of Cover........................................................................................................60
3.1.6 Removal and Re-installation of Base..........................................................................................................64
3.1.7 Troubleshooting Guide for Enclosure.........................................................................................................69

3.2 Photometer ........................................................................................................................................................70


3.2.1 Motor, Photometer (part #994089SP).........................................................................................................71
3.2.2 O-ring, Photometer (part #132059SP) ........................................................................................................74
3.3.3 Photometer Board (part #995574SP) ..........................................................................................................77
3.2.4 Photometer Lamp (part #994615SP) ..........................................................................................................79
3.2.5 Push nut, Photometer (part #125100SP) .....................................................................................................81
3.2.6 Thumbnut, Photometer (part #189621SP) ..................................................................................................81
3.2.7 Troubleshooting Guide for Photometer ......................................................................................................82
3.2.8 Photometer Command List .........................................................................................................................83
3.2.9 Photometer Error Messages ........................................................................................................................84

3.3 Filter Wheel.......................................................................................................................................................85


3.3.1 Filter Label .................................................................................................................................................85
3.3.2 Photometer Filter Replacement / Addition of New Filter ...........................................................................86

3.4 Cuvette Mover ..................................................................................................................................................93


3.4.1 Motor (part #994638SP) .............................................................................................................................94
3.4.2 O-Ring Belt Cuvette (part #132060SP) ......................................................................................................97
3.4.3 Encoder Board (part #995573SP) ...............................................................................................................99
3.4.4 V Mover Motor Controller Board (part #994632SP)................................................................................ 102
3.4.5 Thermistor (part #995564TSP) ................................................................................................................. 103
3.4.6 Opto Right Angle (part #995571SP)......................................................................................................... 105
3.4.7 Cuvette Cover (part #994626SP) .............................................................................................................. 108
3.4.8 Troubleshooting Guide for Cuvette Mover............................................................................................... 108
3.4.9 Cuvette Mover Command List ................................................................................................................. 109
3.4.10 Cuvette Mover Error Messages .............................................................................................................. 110

3.5 Diluter.............................................................................................................................................................. 111


3.5.1 Motor (part #105102SP-46)...................................................................................................................... 112
3.5.2 Belt, small, Diluter (part #132048SP)....................................................................................................... 113
3.5.3 Valve, Diluter (part #105196SP) .............................................................................................................. 113
3.5.4 Diluter Board (part #995575SP) ............................................................................................................... 115
3.5.5 24VDC Supply Board (part #995578SP).................................................................................................. 116
3.5.6 Syringe Cover Diluter (part #189637SP).................................................................................................. 117
3.5.7 Syringe, Diluter (part #105189SP) ........................................................................................................... 117
3.5.8 O-ring & Plunger (part #046005SP) ......................................................................................................... 117
3.5.9 Manifold (part #046007SP) ...................................................................................................................... 118
3.5.10 Removing Diluter from the Chassis........................................................................................................ 118
3.5.11 Diluter Troubleshooting Guide ............................................................................................................... 119
3.5.12 Diluter Command List ............................................................................................................................ 120
3.5.13 Diluter Error Messages ........................................................................................................................... 121
3.6 Probe X............................................................................................................................................................ 122
3.6.1 Black Belt (part #132048SP) .................................................................................................................... 123
3.6.2 Motor with Encoder (part #994618SP)..................................................................................................... 124
3.6.3 Yellow Belt (part #132049SP).................................................................................................................. 125
3.6.4 Probe X Interface Board (part #995569SP) .............................................................................................. 127
3.6.5 Probe X Troubleshooting.......................................................................................................................... 128
3.6.6 Probe X Error Messages ........................................................................................................................... 129
3.6.7 Probe X Command List ............................................................................................................................ 131

3.7 Probe Z ............................................................................................................................................................ 132


3.7.1 Motor, Mixer (part #105061SP-46) .......................................................................................................... 135
3.7.2 Motor, Probe Z (part #994369SP) ............................................................................................................ 137
3.7.3 Belt, Mixer (part #132081SP)................................................................................................................... 139
3.7.4 Belt, Probe Z (part #132080SP)................................................................................................................ 140
3.7.5 Probe Z Fluid Sense Wire (part #993293SP)............................................................................................ 142
3.7.6 Probe Tip Assembly (Part # 994617SP) ................................................................................................... 142
3.7.7 Counterweight Assembly (Part # 994622SP) ........................................................................................... 145
3.7.8 Mixer Assembly (Part # 994620SP) ......................................................................................................... 145
3.7.9 Spindle Bearing Assembly (Part # 994621SP) ......................................................................................... 147
3.7.10 Motor Controller Z Mover (Part # 994636SP)........................................................................................ 148
3.7.11 Optical Fluid Sensor (Part # 995576SP) ................................................................................................. 150
3.7.12 Troubleshooting Guide for Probe Z........................................................................................................ 152
3.7.13 Probe Z Error Messages.......................................................................................................................... 154
3.7.14 Probe Z Command List........................................................................................................................... 156

3.8 Rack Mover..................................................................................................................................................... 158


3.8.1 Motor (part #994640SP) ........................................................................................................................... 159
3.8.2 O-ring Rack (part #132060SP) ................................................................................................................. 159
3.8.3 Encoder Board (part #995573SP) ............................................................................................................. 161
3.8.4 X Mover Motor Controller Board (part #994633SP)................................................................................ 162
3.8.5 Opto Right Angle (part #995571SP)......................................................................................................... 163
3.8.6 Cooling Assembly (part #994344SP) and Graphite Sheet (part #135032SP)........................................... 164
3.8.7 Fan (part #104507SP) ............................................................................................................................... 165
3.8.8 Troubleshooting Guide for Rack Mover................................................................................................... 166
3.8.9 Rack Mover Command List...................................................................................................................... 167
3.8.10 Rack Mover Error Messages................................................................................................................... 167

3.9 Shuttle.............................................................................................................................................................. 169


3.9.1 Motor (part #994641SP) ........................................................................................................................... 170
3.9.2 O-ring Shuttle (part #132062SP) .............................................................................................................. 171
3.9.3 Encoder Board (part #995573SP) ............................................................................................................. 171
3.9.4 Opto Straight (part #995568SP)................................................................................................................ 172
3.9.5 V Mover Motor Controller Board (part #994632SP)................................................................................ 172
3.9.6 Troubleshooting Guide for Shuttle ........................................................................................................... 173
3.9.7 Error Messages for Shuttle ....................................................................................................................... 174
3.9.8 Shuttle Command List .............................................................................................................................. 176

3.10 Chassis ........................................................................................................................................................... 177


3.10.1 Power Supply (part #101110SP)............................................................................................................. 179
3.10.2 Tubing for Serial Number 330 and below (part #135242SP) ................................................................. 181
3.10.3 Barb, Pipe Fitting for Serial Number 330 and below (part #137097SP) ................................................ 183
3.10.4 Luer, Female (part #137102SP).............................................................................................................. 185
3.10.5 Male Nut (part #994651SP).................................................................................................................... 186
3.10.6 Fuse (part #102015SP)............................................................................................................................ 187
3.10.7 Cable, USB 6’ (part #104455SP)............................................................................................................ 187
3.10.8 Cable Assembly, RS1, Ez80, Vmover (part #104047SP) ....................................................................... 187
3.10.9 Cable Assembly, RS2, Vmover, Photometer, Xmover (part #104048SP).............................................. 187
3.10.10 Bottle, 250ml (part #187146SP) ........................................................................................................... 187
3.10.11 Bottles and Drain Tubing Kit (part #994335) ....................................................................................... 187
3.10.12 Fuse Board (part #994631SP)............................................................................................................... 188
3.10.13 Motor Controller, X Mover (part #994633SP) ..................................................................................... 188
3.10.14 Main Board Ez80 (part #994630SP)..................................................................................................... 189
3.10.15 Cooler Board (part #995577SP)............................................................................................................ 189
3.10.16 Motor Controller, V mover (part #994632SP)...................................................................................... 192
3.10.17 Power Cord (US = 137521 & European = 137221).............................................................................. 193
3.10.18 Fan, Chassis (part #104508SP) ............................................................................................................. 193

4. SUB-ASSEMBLY REMOVAL FROM THE CHASSIS ..........................................195


4.1 Removal of Probe X and Probe Z Assembly ................................................................................................ 196

4.2 Removal of Photometer.................................................................................................................................. 197

4.3 Removal of Rack Mover................................................................................................................................. 200

4.4 Removal of Cuvette Mover ............................................................................................................................ 201

4.5 Removal of Wash Cup.................................................................................................................................... 203

4.6 Removal of Shuttle ......................................................................................................................................... 204

4.7 Removal of Diluter ......................................................................................................................................... 205

4.8 Troubleshooting Guide for Chassis............................................................................................................... 206

4.9 Chassis Command List................................................................................................................................... 207

5. PHOTOMETER CALIBRATION VERIFICATION .................................................209


5.1 Photometer Calibration Verification Preparation....................................................................................... 209

5.2 4600 Photometer Reference Kit #046034...................................................................................................... 210

6. ALIGNMENT .........................................................................................................212
6.1 Probe Vs. Wash Cup ...................................................................................................................................... 212
6.1.1 Using the Alignment Window .................................................................................................................. 212

6.2 Probe vs. Rack Mover .................................................................................................................................... 215

6.3 Probe vs. Cuvettes .......................................................................................................................................... 216

6.4 Photometer vs. Cuvettes................................................................................................................................. 216

7. CONTACT INFORMATION...................................................................................217
APPENDIX A – FUNCTIONAL/WIRING BLOCK DIAGRAM ...................................218

APPENDIX B – CHEMWELL-T COMMAND LIST....................................................219

APPENDIX C – REPLACEMENT PARTS ................................................................224


1. INTRODUCTION
1.1 ChemWell-T
ChemWell®-T is a computer controlled instrument system, capable of automating any or all stages of
assay processing that involve these functions:
fluid handling ...............aspirating and dispensing from 2µL to 500µL
incubating ....................cuvette well 37°C, or ambient
reagent cooling………..cools 9° to 12° C below ambient
mixing ..........................on board oscillating (programmable speed)
timing...........................from 1 sec to 24 hours
optical reading .............UV/Visible range
calculating ...................using numerous preprogrammed equations
data storage ................unlimited capacity
reporting ......................with many options and customization to choose from
The system allows you to define and program an unlimited number of customized protocols by
selecting displayed menu options from a Microsoft Windows® software program. This open system
can be programmed to perform any of the colorimetric biochemistry assays or turbidimetric assays
that can be handled using the volumes, temperatures, and wavelengths provided. It has many
possible applications in clinical and veterinarian testing, environmental testing, food and water
analysis, life science research, and may also be used in production processes involving micro
volume dispensing, diluting, incubating, reading, and so on.
Reactions occur in plastic cuvettes instead of sample cups or a carousel. Cuvettes are commercially
available from many sources. Place your reagent bottles and sample tubes into the removable,
adjustable instrument racks. Then program the instrument to pick up from one place, dispense to
another, wash the probe, read the cuvettes, incubate, mix, or whatever you want it to do.
ChemWell®-T is not dedicated for use with any particular chemical reaction, method, or
manufacturer. This gives you many advantages including great flexibility in how you use it. Your lab
decides how to set up your racks and plates, which reagents you will use, how many controls you
will run, how many different applications you want on it, and so on. This also means that the
instrument must first be programmed before each new application.
To assure the quality of clinical information, each new setup must be validated before reporting
specimen results. In some cases the programming, optimization, and validation may have already
been done. Before applying any new clinical reagent system, check first with the diagnostic reagent
manufacturer for specific instructions, validation information, and application tips. You are also
advised to run specimens having known concentrations to verify your instrument setup parameters.
After that, programs can easily be recalled for review, use, change, or deletion by you. You decide
everything including how manual and how automatic you want your system to be.
Awareness Technology, Inc. developed ChemWell®-T instruments and software as tools for the
modern global laboratory. The power and freedom to apply ChemWell®-T, is completely yours.

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 1


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
1.2 Warnings and Precautions

! For in-vitro diagnostics use.


WARNING

! The operation of ChemWell®-T may involve the use of biohazardous


material. Refer to the Owner’s Manual for biohazard warnings.
WARNING

1.2.1 Intended Use


The ChemWell®-T is designed for use in processing general chemistry and turbidimetric
assays. This general purpose instrument is intended to be used by laboratory professionals
who are capable of selecting the appropriate features and options for each specific clinical
application.
• Some diagnostic assays utilize materials which are potentially biohazardous.
• Always wear protective apparel and eye protection while using this instrument.
• Always operate the instrument with the aerosol shield lowered.
• Do not use the instrument in a manner not specified by the manual, or the
protection provided by the instrument may be impaired.
• Probe tips are sharp and may cause bodily injury. Do not place hands or fingers
under the probe while instrument is in operation. Always set the power switch to
OFF (0) before working on the probe. Never touch the probe while the instrument is
operating.
The probe performs a self-clean periodically
while the probe is idle. Keep hands away
! from the probe at all times when the
WARNING instrument is ON (1).

DANGER DANGER: Pinch points, sharp points, and


moving parts - mechanisms may operate
! without warning.

• Solvents such as acetone or thinner will damage the instrument. Do not use
solvents to clean the unit. Avoid abrasive cleaners; the aerosol shield is liquid-
resistant, but is easily scratched.

2 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
• The exterior of the instrument may be cleaned with a soft cloth using plain water. If
needed, a mild all-purpose or non-abrasive cleaner may be used. A 10% solution of
chlorine bleach (chlorine bleach= 5.25% Sodium Hypochlorite) or 70% isopropyl
alcohol may be safely used as a disinfectant. Take special care not to spill liquid
inside the instrument.
Please take time to read this service manual and the operator’s manual carefully before
using the ChemWell®-T instrument. For best results, familiarize yourself with the instrument
and its capabilities before attempting any clinical diagnostic tests. Refer any questions to
your instrument dealer.
Retain the original packing material for future use in the event that the instrument is placed
in storage, shipped to another location, or returned for service. One person can easily lift
unit by placing hands under the side panels and lifting. Instrument weighs 15kg.
ChemWell®-T should be installed on a sturdy, level, surface capable of supporting the
instrument’s weight (15 kg) safely. The instrument should be surrounded by 10 cm (4
inches) clearance on each side, top, front, and back. ChemWell®-T requires no fastening to
the bench top.
1.2.2 General Safety Summary
Review the following safety precautions to avoid injury and prevent damage to this
instrument or any products connected to it. To avoid potential hazards, use this instrument
only as specified.

! Only qualified personnel should perform service procedures.


Contact your dealer to arrange factory training.
WARNING

1.2.3 To Avoid Fire or Personal Injury


• Use Proper Power Cord. Use only the power cord specified for this product and
certified for the country of use.

• Ground the Product. This product is grounded through the grounding conductor of the
power cord. To avoid electric shock, the grounding conductor must be connected to
earth ground. An optional method is to attach a ground strap from the external
grounding terminal on the rear panel of the instrument to a suitable ground such as to a
grounded pipe or some metal surface to earth ground.

• Observe All Terminal Ratings. To avoid fire or shock hazard, observe all ratings and
markings on the instrument. Consult this manual for further ratings information before
making connections to the instrument.

• Do Not Operate Without Covers. Do not operate this instrument with covers and panels
removed.

• Use Proper Fuse. Use only the fuse type and rating specified for this instrument.

• Avoid Exposed Circuitry. Do not touch exposed connections and components when
power is present.

• Do Not Operate With Suspected Failures. If you suspect there is damage to this
instrument, have it inspected by a qualified service person.

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 3


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
• Provide Proper Ventilation. Refer to the installation instructions for details on installing
the product so it has proper ventilation.

• Do Not Operate in Wet/Damp Conditions.

• Do Not Operate In An Explosive Atmosphere.

• Keep Instrument Surfaces Clean and Dry.

! The operation of ChemWell®-T may involve the use of biohazardous material.


Refer to your owner’s manual for biohazard warnings.
WARNING

1.2.4 Safety Terms and Symbols


The following terms appear in this manual:
DANGER indicates an injury immediately accessible as you read the marking.
WARNING indicates an injury hazard not immediately accessible as you read the marking.
CAUTION indicates a hazard to property including the product.
BIOHAZARD indicates biological agents that can cause disease in humans. Lab workers
handling potentially infectious materials must use universal precautions to reduce the risk
of exposure to these agents.

These symbols may appear on the product:

!
WARNING Protective Ground CAUTION BIOHAZARD
Risk of Shock (Earth) Terminal Refer To Manual Risk of Infection

FUSE: For continued protection against risk of fire, replace only


with fuse of the specified type and current ratings. Disconnect
equipment from supply before replacing fuse.

DANGER DANGER: Pinch points, sharp points, and moving parts -


mechanisms may operate without warning.
!

4 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
1.3 Layout
Refer to the figures on the following pages for illustrations of the ChemWell®-T and its
components.

Hazardous line voltages are present behind the AC cover and on the
! power supplies. Always disconnect the external AC power cable before
WARNING servicing the instrument.

ChemWell®-T Component Figure Number

Instrument Exterior Figure 1.3.1

Instrument Exterior (rear view) Figure 1.3.2a

Instrument Exterior (inverted view) Figure 1.3.2b

Instrument Front View Figure 1.3.3

Note: Aerosol shield installed.

Instrument Chassis Figure 1.3.4

Instrument Interior (top view) Figure 1.3.5a

Instrument Interior (front view) Figure 1.3.5b

Sub-assemblies: Figure Number

Cuvette Mover (side view) Figure 1.3.6a

Cuvette Mover (bottom view) Figure 1.3.6b

Rack Mover (back view) Figure 1.3.7a

Rack Mover (inverted view) Figure 1.3.7b

Diluter (side view) Figure 1.3.8

Probe X Mover (back view) Figure 1.3.9

Probe Z Mover Figure 1.3.10

NOTE: Probe X and Z Movers are


combined and are responsible for the
probe movement.

Shuttle (front view) Figure 1.3.11a

Shuttle (rear view) Figure 1.3.11b

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 5


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Layout (Continued)

Sub-assemblies: (Continued) Figure Number

Photometer (front view) Figure 1.3.12a

NOTE: Houses the lamps, filters, and is


responsible for photometric readings.

Photometer (rear view) Figure 1.3.12b

Prime Bottle Figure 1.3.13

Wash Cup Figure 1.3.14

Universal Rack Figure 1.3.15

Cuvette Holder Figure 1.3.16

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©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
1.3.1 Instrument Parts and Controls

Top

Shield

Cover

Base

Figure 1.3.1 Instrument Exterior

Top (rear view)

Power Connection

Ground Terminal

Cover (rear view)


USB Connection

Base (rear view)


Drain Connection
ON / OFF Switch

Figure 1.3.2a Instrument Exterior (rear view)

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 7


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Instrument Parts and Controls (Continued)

Speaker

Air Exhaust

Air Intake

Photometer
Fan

Figure 1.3.2b Instrument Exterior (bottom view)

CAUTION! DO NOT BLOCK FAN OPENING,


AIR INTAKE OR AIR EXHAUST

8 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Instrument Parts and Controls (Continued)
Top

Shield

Probe Tip

Universal Rack
500µl Syringe

Cuvette carrier

Prime Bottle Cover

Base
Figure 1.3.3 Instrument Front View – complete with shield

Reagent cooling only in


positions 1 and 2 via the Cuvettes may
self-enclosed Peltier incubate and be
cooling system heated to 37.0°C

1 2 3 4 5

Figure 1.3.3a Cooling in reagent area only; cuvette incubation area pinpointed

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 9


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Instrument Parts and Controls (Continued)

X-Mover PCB Fuse Board


Power Supply
Cooler PCB
V Mover PCB

Prime Bottle
Ez80 Board

Chassis

Figure 1.3.4 Instrument Chassis

Circuit boards mounted in housing

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©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Instrument Parts and Controls (Continued)

Figure 1.3.5a Instrument Interior (top view) shown out of enclosure and with Probe Z
and Mixer cover removed

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 11


©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Instrument Parts and Controls (Continued)

A
B

G
D

Figure 1.3.5b Instrument Interior (front view)

Sub Assembly
A Probe Z
B Probe X
C Rack Mover
D Photometer
E Wash Cup
F Shuttle
G Diluter/Syringe
H Cuvette Mover (heated)

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restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Cuvette Mover

B
A
C D

Figure 1.3.6a Cuvette Mover (side view)

E F
G

Figure 1.3.6b Cuvette Mover (inverted view)

Components of the Cuvette Mover


A Home Switch
B Lead Screw
C Motor
D Encoder
E Thermistor
F Heating Resistors (6)
G Belt

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restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
Rack Mover

Figure 1.3.7a Rack Mover (back view)

C D
E

F
G
H
I

Figure 1.3.7b Rack Mover (inverted view)


Rack Mover
A Motor F Hot Block
B Belt G Cold Block
C Home Switch H Peltier
D Cooling Fan I Lead Screw
E Encoder

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Diluter

A
B
C

D
E

F G
H
Figure 1.3.8 Diluter (side view)

Diluter Components
A Manifold
B Syringe
C Guide Rod
D Belt
E Valve
F 24VDC Supply
G Diluter PCB
H Motor

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 15


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Probe X Mover

A B C

E F G I
H

Figure 1.3.9 Probe X Mover (back view)

Probe X Mover Components


A Large Belt
B Igus Chain
C Guide Rod
D Small Belt
E Mid position Switch
F Probe X Interface PCB
G Home Switch
H Encoder
I Motor

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Probe Z Mover

B
D

C G

Figure 1.3.10 Probe Z Mover (side view)

Probe Z Mover Components


A Probe Z Home Switch
B Home Flag
C Mixer Motor
D Fluid Sensor PCB
E Lead Screw
F Mixer Belt
G Counterweight Assembly
H Guide Rod

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Shuttle
Shuttle Motor

Belt

Encoder Wheel

Figure 1.3.11a Shuttle (front view)

Comb Switch

Lead Nut

Home Switch

Comb Shuttle Truck

Figure 1.3.11b Shuttle (rear view)

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Photometer

Filter Wheel Belt

Analog Board

Filter Wheel
(located behind
board)

Figure 1.3.12a Photometer (front view – shown without cover)

Transfer Block

Lens

Lamp

Lens Bracket

Thumb nut

Lamp Assembly

Filter Wheel Motor

Digital Board

Figure 1.3.12b Photometer (rear view)

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 19


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Prime Bottle

Figure 1.3.13 Prime Bottle 0.25L

Wash Cup

Wash Cup

NOTE: Mounting style for


Wash Cup may vary.

Figure 1.3.14 Wash Cup

20 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011


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Reagent and Sample Rack

Position 1
A
D
Position 2 C
B
Position 3 E

Position 4

Position 5

Figure 1.3.15 Universal Rack

Sector Size/type
A 30ml bottle holder
B 15ml bottle holder
C Mixed bottle holder
D 2ml vial holder
E Primary tube sample holder will accommodate 50mm,
75mm, or 100mm tubes

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Cuvette Holder

1 23
45

Express 550 Cuvettes A


B
C
D
E
F Handle
G
H

Figure 1.3.16 Cuvette Holder

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Diluter PCB Layout

EPROM Memory

RS-232 Control

Power Input

Stepper Valve
Motor

J5 Strap for full


step operation

24V Power Supply

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 23


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USB
Master Controller (eZ80 Layout)

Heater

Lamp

Heater

Power Input

RS-485

Audio

Flash
Download

Diluter

Heartbeat
LED

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Motor Controller (3 Units) – Board Stack
Top PCB

Bottom PCB

Board to Board
Interface

Temperature
Position Encoder Sensor Input
Inputs
Optical
Sensor
Inputs
Optical Sensor
Inputs

Position
Temperature Encoder
Sensor Input Inputs

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 25


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Photometer Digital PCB

RS-485 Communications Bus

Flash Download

Filter Wheel Motor

Board-to-Board
Connector

26 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011


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Photometer Analog PCB
Board-to-Board Connector

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 27


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1.4 Technical Specifications

Technical Specifications for ChemWell®-T


Overall
Typical throughput up to 100 tests per hour
Typical reaction volume 200µL or less
Dimensions 53cm W x 50cm L x 45cm H, approx. weight = 15kg (38lbs)
Reagent and Sample Dispensing
Capabilities dilutions, predilutions, dispensing reagents
Pumps Single syringe pump, sized: <500µL
Probe 316 stainless for maximum reagent compatibility, level sensing
Minimum and maximum 2µL - 400µL
volume
Precision <1%
Maximum number of 20
specimens (including
calibrators and controls)
Maximum number of reagents Typically 20
Reaction vessel Express 550 Cuvette
Instrument bottle 0.25L Prime Bottle
Incubating, Timing and Temperature Control
For Chemistries each cuvette is timed separately
Thermal control Cuvette holder 37°C, or ambient room temperature
Reagent rack cools 8° C to 12° C below ambient through
Peltier thermoelectric modules.
Sample rack is not temperature controlled.
Reading
Optical design Reads absorbance in one channel, NIST traceable calibration,
user selects monochromatic or bichromatic results
Light source Tungsten halogen lamp
8-position filter wheel 340, 405, 505, 545, 580, 630, plus two blanks
Interference filters long life, hard coat, ion assisted deposition, +/- 2nm, 10nm
typical half bandpass
Linear range 0.0 to 3.0A
Photometric accuracy • ± (1% of the reading +0.005A from 0 to 1.0A)
• ± (2% of the reading +0.005A from 1.0 to 3.0A)
Software
Format USB and Internet upgrades
Operating systems Microsoft Windows® XP, Vista or Windows 7
Minimum system Microsoft Windows® XP 256 RAM; USB port

Main menu options Patients, New Job, Job, Setup


Secondary menu options Create/edit protocols, import/export assays, data, etc.,
Control, Run, Setup
Calculation modes Absorbance, single standard, factor, fixed time kinetics,
kinetics by standard or factor, multi-calibrator point-to-point,
linear regressions, cubic spline, 4-parameter logistic and
percent absorbance
Self monitoring modes Reagent volume, filters, mechanical function, and more
QC options Store control data, print Levey-Jennings or QC range plots,
calculate SDs
28 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011
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Technical Specifications for ChemWell®-T
USB port USB cable provided
Power
Voltage range 100-250VAC
AC input power Approximately 175 watts
Frequency range 50-60Hz
Power maximum 250 watts
Installation category CAT II
Environmental Conditions for Safe Operation
• Indoor use
• Altitude up to 2000 m
• Temperature 5°C to 40°C 1
• Humidity 80% for temperatures up to 31°C decreasing linearly to 50% humidity at 40°C.
• Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage
Recommended Environmental Conditions
Recommended operating 18-35°C
temperature
Recommended operating less than 85%
humidity
Certifications
• Pending

Design and instrument specifications are subject to change without notice.


1
Although it may be safe to operate in these conditions, it may not be suitable for the performance of your
tests. Check with your supplier.

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2. PRINCIPLES OF OPERATION
2.1 Electronic Hardware Description
2.1.1 Master Controller (eZ80) PCB
The Master Controller is located towards the back of the machine on the right hand side.
The Master Controller consists of an eZ80 microprocessor that is programmed to
communicate with the external computer, execute commands from the external computer
and control six motors used for motion control, a photometer and a diluter module.

EZ80 Master Controller PCB

The main components of the Master Controller are the eZ80 microprocessor, 2MBytes
Flash memory, 1MBytes of SRAM, 64KByte of EEPROM parameter storage, 16 bit audio
output, RS-485 communications bus used to control the motor controllers and the
photometer, RS-232 interface used to communicate with the Diluter, +5V stabilized
photometer lamp supply and a switched heater supply.

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USB

Heater

Lamp

Heater
Power
Input

RS-485

Audio

Flash
Download

Diluter

Heartbeat
LED

Figure 2.1.1-1 Master Controller (eZ80) PCB

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Master Controller (eZ80) PCB (Continued)

The I/O connections are shown in Figure 2.1.1-1. J2 is a flash download port that is used
with the Zilog flash downloader system. This is the mechanism to program the flash
memory during the PCB assembly. The flash memory will be able to be programmed
through the computer USB interface once the boot loader is programmed into the flash for
the first time.

Main power originates from the fuse board and is connected to the Master Controller
through several cables located on the back of the PCB. J10 supplies +12V and ground,
J11 supplies +5V and ground, J12 supplies +3.3V, +5V, and ground, and J15 supplies
+5V and ground. The UART to USB bridge circuit is contained within the Master
Controller PCB. UART1 is multiplexed via analog switch to control either the RS-485
bus or the Diluter RS-232 interface.

The Master Controller is also capable of switching power devices such as the Cuvette
heaters and the Photometer lamp. These power switching devices are controlled by the
eZ80 but are also logically connected to a dead man timeout circuit. The eZ80 has to
periodically strobe the dead man circuit in order to keep the switched powered outputs
enabled. If the eZ80 fails to strobe the dead man circuit, then the one-shot times out, and
disable all the switched power outputs. This protects from having a switched power output
failing in the “on” state.

The 16 bit Audio consists of an audio data digital-to-analog converter (DAC), op amp
buffers, a gain control DAC, and a power amp used to power the speaker mounted on the
bottom of the unit. The eZ80 transmits audio data via the SPI bus to the audio DAC. The
gain control DAC operates as a variable attenuator in order to not overdrive the audio
power amp since the audio power amp gain is set to 15. Audio sample rate is set by the
timing of the ez80 and is usually in the 8 KHz range.

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2.1.2 Motor Controller PCB
The Motor Controller PCB is a dual axis motor controller capable of controlling two
separate motor and sensor interfaces. The Motor Controller (3 units – board stack) PCB
is shown in Figure 2.1.2-1.

Bottom PCB

Board DC Power
to Input
Board
Interface

Motor 1
Output

Motor 0
Output

Cooler Board Control


(X Mover Only)

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Motor Controller PCB (Continued)

Top PCB

Temperature Optical Sensor Position Encoder


Sensor Input Inputs Inputs

Board
to
Board
Interface
RS-485

Flash
Download

Position Encoder Optical Sensor Fluid Sense Temperature


Inputs Inputs (Z Mover only) Sensor Input

Figure 2.1.2-1 Motor Controller (3 units board stack)

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Motor Controller PCB (Continued)

The X Motor Controller consists of a Z8 microprocessor, RS-485 communications interface,


two temperature sensor inputs, a motor driver circuit capable of driving two DC motors, and
a switched +12V power output to control the cooler board.

Power input is from the fuse board via the disk drive (FDD) connector shown in Figure
2.3-4 Motor Controller Connector Pinout. The motor driver circuit and the switched
+12V power output are controlled by the Z8 but is also logically connected to a dead
man timeout circuit. The Z8 periodically strobes the dead man circuit in order to keep
the motor driver circuit and switched powered output enabled. The Z8 +3.3V power is
derived from the +5V input by voltage regulator and is indicated by LED (D24). Each
of the two motor interfaces consists of an optical encoder wheel interface and three
optical location sensor inputs. One sensor input located on J10 (ONLY on the Z-Mover
Controller) is used as a fluid sense input. This only applies to the Z-Mover since parts
are configured differently from other movers. The RS-485 communication bus is a
multi-drop configuration and must be terminated at the end of the cable so as to reduce
reflections.

Motor Controller PCBs are used in three different locations. The X-Mover PCB is located at
the back on the left side. This Motor Controller operates the X axis and the Reagent Rack
motion control. It also controls the Cooler within the Reagent Rack via the switched +12V
output. In this particular case, the switched output is used only as a signal to control the
Cooler and does not supply the Cooler power. The Z-Mover PCB is located on the Z mover
and controls the Z axis and the mixer motor. It also connects to the probe to provide fluid
sensing. The V-Mover PCB is located on a bracket support on the right side of the unit.
This unit controls the Read Shuttle and the Cuvette Mover.

X Motor Controller PCB

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2.1.3 Photometer PCB
The Photometer PCBs consist of a board stack that is divided into an analog PCB that
resides inside the photometer enclosure and a digital PCB that resides on the back of the
photometer wheel bracket.

The PCBs are interconnected via a dual row header through a slot in the back of the
wheel bracket. Analog test points are brought out to the digital PCB in order to have
access to the test points while the photometer cover is installed. The digital board
contains a Z8 microprocessor that is programmed with photometer operational code and
RS-485 communications interface code. The photometer is a self-contained unit such
that the only external connections needed to operate is the communications bus and
power. The lamp power comes from a stabilized switched 5V source on the Master
Controller. Photometer is powered by +5V from the fuse board via the 2-pin cable
connected at J11 near the center of the outer PCB.

The filter wheel is rotated by a small DC motor connected to the wheel by a rubber belt.
The wheel speed is electronically controlled to four revolutions per second. As the wheel
turns, the filters mounted in it pass between the light source and the photo detector, the
reading is taken when the filter passes through the light beam.

Optical gates (beam interrupt switches) are used to control the filter wheel speed and take
readings. Normally the infrared light from the LCD side of the gate is blocked, but when the
small hole in the filter wheel passes by, the light passes through and the microprocessor
receives a signal.

Photometer Digital PCB


Filter Wheel Motor (FWM)

+5 V Power Input
Board
to Flash Download
Board
Interface

Test Header

RS-485
Figure 2.1.3-1 - Photometer Outer PCB

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Photometer PCB (Continued)

Photometer Analog PCB

Figure 2.1.3-2 - Photometer Inner PCB

Board
to
Board
Interface

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 37


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2.1.4 Diluter PCB
The Diluter PCB is a single syringe design, a Z180 microprocessor, 32KByte EPROM,
8Kbyte SRAM and an 8255 PC type PIO circuit. The PIO indicates “processor alive” status
through LED (D11) and controls the direction, clock and reset signals on the L297D stepper
motor circuit. The PIO also controls the valve through buffer transistor. The two optical limit
detectors are also sensed by the PIO through phototransistors Q2 and Q3. The L297D
provides the four phased control signals to the L298N motor driver interface.
A dead man circuit provides protection in the event that the Z180 stalls and the stepper
motor output is being driven. Communication is via a RS-232 interface circuit. This
dedicated link is cabled directly to the Master Controller RS-232 serial interface. Power
input originates from the fuse board, +12V is connected to J6 and provides power for the
valve. +12V is also transferred to a separate power supply PCB connected directly to the
Diluter PCB through board-to-board header J2. This +24V power supply converts +12V to
+24V used by the stepper motor. Additionally, this PCB includes a +5V voltage regulator to
provide +5V for the logic circuitry on the Diluter PCB.

Diluter PCB Layout

EPROM Memory

RS-232 Control

Power Input

Board-to-board to
+24V Power
Supply PCB
Stepper
Motor
Valve

J5 Strap for full


step operation
Figure 2.1.4-1 Diluter PCB

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2.1.5 PCB Light Diagnostic Guide
USB

Heat ON:
Warm-up:
Full ON
Regulating:
Flashing

System Status:
ON: Normal
OFF: Error

Main processor
communicating:
Power light: Flashing red
Steady Any action
green light causes faster
flashing

Figure 2.1.5-1 Master Controller (eZ80) Layout

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 39


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PCB Light Diagnostic Guide (Continued)

Z Motor Controller

Probe Z Home: Green lights: Z Mover Probe Z Encoder:


Steady green block Always ON connected: Steady green when blocked
OFF when open Steady green light OFF when open

Communicating:
Slow red
flashing

Performing Power ON:


action: Steady
Fast red flashing green light

Heartbeat (Time
Loop Flashing)
Main Code
Lamp Flashing

Green light: Fluid Sense: Z Probe jam: Spindle wheel home:


Always ON Steady green ON when green when blocked
jammed OFF when open

Figure 2.1.5-2 Z Motor Controller

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PCB Light Diagnostic Guide (Continued)

X Motor Controller

Probe X Reference:
Probe X Encoder:
PROBE X Probe X Home: Steady green light Green light:
Steady green block Always ON Steady green when blocked
when blocked
OFF when open OFF when open
OFF when open

RX/TX
Communications:
Fast red flashing

Performing Power ON:


action: Steady
Fast red flashing green light
-Motor Control
Active
-Communication
Reset

Heartbeat (Time
Loop Flashing)
Main Code Lamp
Flashing

RACK
MOVER

Rack Mover encoder: Green light: Rack Mover over travel: Rack Mover home:
Steady green when blocked Always ON Steady green when blocked Steady green when blocked
OFF when open OFF when open OFF when open

Figure 2.1.5-3 X Motor Controller

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PCB Light Diagnostic Guide (Continued)

V Motor Controller

Comb position:
SHUTTLE Home Opto: Green light: Shuttle Encoder:
Steady green light
Steady green when Always ON Steady green when blocked
when blocked
blocked OFF when open
OFF when open

Communication:
Slow red flashing

Performing
action: Power ON:
Fast red flashing Steady
green light

Heartbeat (Time
Loop Flashing)
Main Code Lamp
Flashing

CUVETTE
RACK

Cuvette Mover Encoder: Green light: Cuvette Mover over travel: Cuvette Mover Home:
Steady green when blocked Always ON Steady green when blocked Steady green when blocked
OFF when open OFF when open OFF when open

Figure 2.1.5-4 V Motor Controller

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PCB Light Diagnostic Guide (Continued)

Diluter PCB Layout

Power ON

Diluter processor
connected:
Slow blinking
green light

Diluter valve
open:
Steady green

Diluter valve
closed: Light OFF
Figure 2.1.5-5 Diluter PCB

Cooler PCB

Cooling ON:
Steady green light
Cooling OFF:
No light

Figure 2.1.5-6Cooler PCB

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PCB Light Diagnostic Guide (Continued)

Fuse PCB
NOTE: Lights on to indicate continuity of fuses.

Fuse
Lights

Fuse
Lights

Fuse
Lights
Figure 2.1.5-7 Fuse PCB

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PCB Light Diagnostic Guide (Continued)

Photometer Digital PCB

Flashing when filter wheel is


turning

Power ON:
Steady green light

Figure 2.1.5-8 Photometer Digital PCB

ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 45


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2.2 System Communication Description
The ChemWell®-T architecture is a distributed processor control system that is connected
in a high speed multi-drop half duplex configuration. The distributed nature of the system
allows for modularity which benefits system component replacement and testing of
individual modules. The three motor controller boards have similar functionality, but are
each populated with different parts and require different configuration firmware. Because of
this, the three controller modules are not interchangeable and care must be taken to
ensure they are installed in the correct locations. The physical media is a RS-485
differential serial link which is commonly used in a high noise industrial environment.

The system consists of a master controller board, three dual axis motor control slave
boards, a Diluter and a slave photometer board set. The motor controllers are configured
as follows:
a. X Controller: controls the X mover and Reagent Rack
b. Z Controller: controls the Z mover and mixer.
c. V Controller: controls the Cuvette mover and Read shuttle.

The single syringe diluter is controlled via a RS-232 serial port.

2.2.1 System Protocol Description


The system RS-485 bus operates in a half duplex mode. Slaves only respond to the
master controller when addressed. The master sends packetized data to the slaves.
Error detection and a retry mechanism are employed.
2.2.2 Slave Programming
These Motor Controller boards contain an analog-to-digital converter which reads a
voltage unique to each module and will identify them as an X Controller, V Controller,
Z Controller, or Photometer. These boards can not be programmed to function as a
different module. Programming code is downloaded through the master controller
board and to the appropriate module. The master controller stores the slave module
programming. If a new unprogrammed slave is connected to the bus, then the
master will detect the event and download the appropriate program to the slave. The
unprogrammed modules have a resident boot loader that allows it to download code
in this fashion. Additionally, new code can be downloaded via the USB connection in
order to update the firmware for the master controller. This firmware update will
contain current firmware for all slave modules.

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2.3 Power Subsystem
A 250 watt universal input PC type power supply serves as the main power source to the
system (reference Power Supply Block Diagram in Figure 2.3-1a and Figure 2.3-1b). The
power cables are terminated with the normal PC type connectors. The main power supply
(10) bus through the standard ATX motherboard connector. The pinout is shown in Figure
2.3-2. The stock 12V2 DC connector is shown in Figure 2.3-3. The stock peripheral
connectors are shown in Figure 2.3-4.

Figure 2.3-1a Power Supply Block Diagram


®
ChemWell -T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011 47
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Power Subsystem (Continued)

Power Supply

V Mover PCB

Ez80 Board

Chassis

Figure 2.3.1b Instrument Chassis – Power Supply Identified

V mover controller PCB

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Figure 2.3-2 Power Supply Motherboard Connector Pinout

Figure 2.3-3 Cooler PCB Connector Pinout

Figure 2.3-4 Motor Controller Connector Pinout

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Fuse PCB

Fuse PCB

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Encoder

Cooler PCB

Fluid Sense (located on Probe Z Mover)

Opto

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Probe X Encoder

Thermistor PCB

X Interface

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2.4 Computer to ChemWell-T Communication

USB RS-485

Master Slave Slave

CHEMWELL-T

Figure 2.4-1 Computer to ChemWell®-T Communication


In addition to the commands originating from the computer, Master and Slaves
communicate to coordinate a variety of functions. This communication is transparent and
the ChemWell®-T can be treated as a unified instrument.

2.4.1 Firmware versus Software


The internal software, usually referred to as the firmware, is responsible for the direct
control of the mechanisms and other systems.
The “software” refers to the Microsoft Windows® program running on the external PC. This
is the program that interacts with the user and controls the ChemWell®-T.
Fundamentally this software breaks down all operations into a set of commands which the
instrument can execute. For an explanation of these commands refer to the Section on
Troubleshooting.
The version of the software can be identified by selecting “Help/About...” from the
ChemWell®-T pull-down menu. In this dialog you will see a message in the format “Version
x.xx / Build yyyy”. The Build Number is the primary means of identifying the software
version.

2.5 Motion Control


2.5.1 Mixer
Mixing is accomplished by rotating the probe within the cuvette. Mixing speed can be
controlled via a user parameter.
2.5.2 Probe X
A bidirectional DC motor, under microprocessor control, pulls a slide via a nylon belt and
nylon pulleys. The motor is dedicated to X motion of the probe. Probe X has a two-
phased encoder wheel which creates pulses when passing between a reader, providing
quadrature tachometer pulses to the microprocessor. The one phase of the index pulley is
used by the software to determine direction and accurately track the probe’s position.
Another phototransistor, positioned toward the right side of the probe X movement, is used
to verify and if necessary, reset the probe count additionally. Another phototransistor
indicates the home position to the microprocessor. LED on the Motor Controller board
provides visual feedback to the service technician of the tach pulses and home positions.

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2.5.3 Probe Z
A bidirectional DC motor, under microprocessor control, turns an ACME lead screw via
neoprene belt and nylon pulley. The motor is dedicated to the vertical Z motion of the
probe. Probe Z uses a pulley with an attached aluminum code wheel that has index slots
which create pulses when passing between an IR LED and a phototransistor, providing
tachometer pulses to the microprocessor.

Probe Z mover (front view)

Probe Z mover (back view)


Additionally other phototransistors indicate the home position to the microprocessor. LED
provides visual feedback to the service technician of the tach pulses and home positions.
The Z Mover Motor Controller board holds the motor driver and interfaces to the Probe
Sense board.

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2.6 Syringe Pump
The syringe pump is an OEM component of the ChemWell®-T consisting of a syringe drive
and a valve which are capable of independent operation through its own microcontroller
board. The pump module has its own internal command set, with communications between
the syringe pump and the ChemWell®-T Master Controller (eZ80) PCB via serial bus. A
feeder tube goes from the Prime bottle to the Diluter manifold. The connector at the diluter
manifold is color-coded black.
Volume pipetting is accomplished by the color-coded red connector that goes from the
Diluter manifold to the Probe tip. The motor demands 24 volts (maximum) from the power
supply. Maximum output from the power supply is 15 volts. To achieve the 24 volts, a
separate PCB 24v converter is attached to the Diluter PCB.
2.7 Photometer
The photometer consists of a mechanism with its own electronics and additional calibration
and analog to digital conversion circuitry on the Photometer Analog Board. The mechanism
contains the light source, filters, detector and electronics. Light from a tungsten-halogen
lamp passes downward though the cuvette containing its sample. Inside the sealed box is a
rotating filter wheel and a photodiode detector. The filter wheel contains positions for eight
interference filters of various wavelengths, and is speed controlled to approximately three
rotations per second under software control.
While in motion, as each filter passes in front of the photo detector, an infrared optical
switch triggers a sampling of the peak voltage by the Photometer Digital Board. The
voltage is then fed to the comparator which compares the sampled voltage to the output of
an exponential capacitor decay circuit. The pulse at the output of the comparator is timed
internally by the Z8 processor in the Digital Board. The counts are read by the
microprocessor and this completes the analog to digital conversion.
The photo detector output is proportional to the intensity of the light, whereas the width of
the positive phase of the comparator output is proportional to the absorbance. The
resistance across the log cap (RC decay) determines the base of the log (10 for
absorbance) and is used to adjust the low-end absorbance calibration (gain). Another
electronic potentiometer is used to adjust the high-end absorbance (offset) of the channel.

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2.8 Computer Connections
The computer (PC) communicates with ChemWell®-T via a Type B USB port.
2.9 Computer Software Interface
Reference the 4600 Operator’s Manual for information regarding the software.
ChemWell®-T uses the standard Windows® dialogs. If you are unfamiliar with these controls
and how to use them, please refer to your Windows® documentation. All of the ChemWell®-T
functions are available from the pull-down menus at the top of the program. Some of the more
common functions are also available from the main menu.
2.10 Instrument Routines
This dialog gives you easy access to common instrument routines. The following options are
available:
Note: The “Start of Day” and “End of Day” functions are also available from the “Run” menu.
Start of day ........................Prepares the instrument to be run by turning on the photometer
lamps and washing the probe.
End of day .........................Prepares the instrument to be shut down at the end of the day by
turning off the photometer lamps, washing the probe (future
feature).
Wash probe .......................Wash the probe.
Prime Syringe ....................Prime the syringe with fluid from the prime bottle.
Cuvette Heat ON ...............Turns cuvette heater ON.
Cuvette Heat OFF .............Turns cuvette heater OFF.
Rack Cooling ON...............Turns rack cooling ON.
Rack Cooling OFF .............Turns rack cooling OFF.
Read Cuvettes...................Reads individual or rows of cuvette.

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3. SERVICE PROCEDURES
WARNING! Set the power switch to OFF (0) position before performing
! any service work on this instrument.

3.1 Enclosure
ENCLOSURE – this section contains Spare Parts List, Enclosure Removal Procedures,
Service Instructions, Troubleshooting Guide

The Enclosure covers the Chassis and moving components. It has been designed to be rugged
and easy to service. To access most of the components mounted to the chassis, it will be
necessary to remove the Syringe Diluter Cover, Cuvette Cover Assembly, Top, Shield
Assembly and Cover. Occasionally, the Base may have to be removed to access wires or
fasteners located on the underside of the Chassis. Access holes for the Photometer fasteners
have been provided through the Base to allow the Photometer to be removed without having to
remove the Base.

Service: The Enclosure is designed to give years of trouble free service. There are a few items
that may need attention or replacement during the life of the instrument. They are:

1. Cuvette Cover Assembly (part #994626SP)

2. Syringe Diluter Cover (part #189637SP)

3. Shield Assembly (part #994625SP)

4. Drain Barb T (part #137006SP)

5. Tubing (part #135237SP)

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3.1.1 Enclosure Removal Procedures

Shield Assembly Top Cuvette Cover Assembly Syringe Diluter Cover

Cover Base

Removal of the Enclosure parts will be described in detail in the following pages.
Caution: Risk of damage to the Probe Tip could occur. It is advised to remove the
Probe Tip before removal of Cover or Base!

3.1.2 Removal of Cuvette Cover Assembly (part #994626SP) and Syringe


Diluter Cover (part #189637SP)

1. The Cuvette Cover Assembly sits over the Cuvette Mover and can be lifted off easily
and placed out of the way. The Syringe Diluter Cover is held in place with 2 pins in the
rear of the cover. Lift the cover straight up to remove it.

3.1.3 Removal and Re-installation of Top (needed to remove the Shield)

1. The Top sits in a groove molded into the Cover. It is held in place by 3 thumb screws
that apply slight pressure to the outside surface. To remove the Top, loosen the 3
thumb screws and lift the Top straight up and out of the groove and away from the
thumb screws and brackets.
2. To re-install the Top, position the Top over the side and back brackets and align the
slots with the 3 thumbs screws. Slide the Top down over the 3 thumb screws until it
rests all the way down in the molded groove.
3. Carefully re-tighten the thumb screws. Use only light hand tightening pressure on
these 3 thumbs screws.

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Loosen the 3 thumb screws and lift Top straight up

(View looking down at instrument from the top)

3.1.4 Removal and Re-installation of the Shield Assembly (part #994625SP)

1. Remove the Top as described above.


2. Close the Shield Assembly so it is resting on the front surface of the instrument.

Shield Assembly

3. Remove the 2 shoulder screws with a flat blade screwdriver.


4. Carefully slide the Shield Assembly away from the pivot posts and remove the Shield
Assembly from the instrument.
5. To re-install the Shield Assembly, place the assembly on the front of the instrument
with the handle facing forward.
6. Slide the sides of the Shield Assembly over the pivot brackets until the pivot holes are
in line with the holes in the pivot.
7. Re-install the 2 shoulder screws through the Shield Assembly holes and into the pivot
bracket holes. Tighten with a flat blade screwdriver.

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3.1.5 Removal and Re-installation of Cover

1. To remove the Cover, the Cuvette Cover Assembly, Syringe Diluter Cover, Top and
Shield Assembly must first be removed. If there is a Rack assembly in the instrument,
it should also be removed as well as the Cuvette Carrier if it is in the instrument.
2. With the Cover and Top removed, the 7 screws that hold the cover can easily be
accessed. Remove the 4 pan head screws and flat washers that fasten the Cover
down from the top. One of these screws also holds the sheet metal Cuvette Cover
Rear Cover. Please note that these 4 screws are longer than the other 3 that will
be removed next. The longer screws must be used in the top surface to prevent
threads from pulling out!

Remove the 4 pan head screws and washers that fasten the Cover down from the
top
(Cuvette Cover Rear Cover)

Remove the 2 pan head screws and washers from the angled surface on the
right side of the Cuvette Mover

3. Remove the 2 pan head screws and washers from the angled surface on the right side
of the Cuvette Mover.

4. Remove the screw and flat washer on the vertical surface on the left side of the Rack
Mover.

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5. Unscrew the RED fitting from the rear of the Manifold. This fitting may be difficult to
unscrew but can be made easier by removing the Shuttle Cover.
6. To remove the Shuttle Cover, remove the top, right side pan head screw and washer,
then loosen the screw below it and the screw on the left side of the cover. Lift the
Shuttle Cover up and off the 2 screws.

Shuttle Cover Unscrew RED fitting from the rear of the Manifold

To remove the Shuttle Cover, remove top, right side pan head screw and washer

7. Remove the Prime Bottle and unscrew the Luer fitting from the fitting on the instrument.
Be careful not to let the water siphon out of the bottle while it is off the instrument.
8. Slide the Probe Z to the left with the Probe tip approximately over the slot in the Rack
Mover Deck. This will allow the Cover to clear the Probe Z when it is lifted off.
9. The Cover is now ready to be removed. Carefully lift up on the cover to separate it
from the seam where it mates with the Base.
10. Use both hands to lift the left side up while watching the right side for clearance and
holding it up just slightly. The left side should lift up past the Probe X rail bracket and
then the right side can be maneuvered out from under the right side of the Probe X rail
bracket. It should be noted that the Cover has rails on the inside surface where it
mates with the Base. These rails insure a rigid seam when mated to the Base. Care
must be taken during re-installation of the cover to insure the rails fit properly with the
Base.

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Use both hands to lift the left side up while watching the right side for clearance and
holding it up

The left side of cover is lifted up past the Probe X rail bracket first and then the right
side can be maneuvered out from under the Probe X rail bracket.

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Inside of Cover with rails to insure a rigid seam when mated to the Base

11. Re-installation of the Cover is the reverse of removal. Position the Probe Z so that the
Probe tip is approximately over the slot in the Rack Mover Deck. Hold the Cover with
both hands by the sides and with the front of the instrument facing forward, hold the left
side higher than the right side and maneuver the right side of the Cover under the
Probe X rail bracket. The RED fitting and tubing needs to be on the top of the Cover
and may need to be put through the right side opening in the cover while installing the
Cover. The Manifold tubing from the Prime bottle should go into the provided cutout
and then the left side of the Cover should slide past the left side of the Probe X rail
bracket.
12. Once the Cover is positioned over the Base, it will be necessary to push the Cover
down onto the Base flange. Remember, the Cover has rails on the inside of the front
and sides that will need to capture the flange on the Base. It may be necessary to work
from one corner and then out and around the perimeter of the Cover in order to get it
fitted properly to the Base.
13. Check the Cover at the right rear of the Cuvette Mover to make sure the Cover is not
resting on top of the Cuvette Mover deck.

Make sure
cover is
down at
this critical
point.

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14. Once the Cover is correctly fitted to the Base, the 4 long screws and washers should be
re-installed down from the top surface. Be sure to re-install the Cuvette Mover Rear
Cover when re-installing the 4 top screws.

15. Re-install the 2 shorter screws and washers on the angled surface along the right side
of the Cuvette Mover. (see step #3)

16. Re-install the remaining short screw and washer on the vertical surface on the left side
of the Rack Mover. (see step #4)

17. Re-install the RED fitting into the back of the Manifold.

18. Re-install the Shuttle Cover if it was removed.

19. Re-install the Prime bottle and re-connect the Luer fitting.

20. Re-install Shield Assembly, Top, Cuvette Carrier, Cuvette Cover Assembly, Syringe
Diluter Cover, and Rack.

3.1.6 Removal and Re-installation of Base

1. To remove the Base, it will be necessary to first remove the Cuvette Carrier, Cuvette
Cover Assembly, Syringe Diluter Cover, Rack, Top, Shield Assembly and Cover.

2. With the Cover removed, there is now access to the Chassis. The entire Chassis can
be separated from the Base. Start by unplugging the switch from the Fuse board and
pushing the switch out of the Base.

Unplug the switch from the fuse board and push the switch out of the Base

3. Remove the thumb screw that is used for an earth ground. It screws into the power
supply.

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4. Remove the drain tee and tubing.

Remove drain tee Remove thumb screw that is used for an earth ground (part #137006SP)

5. Gently tilt the instrument to access the center screw and large flat washer on the
underside of the instrument. Remove the center screw and flat washer.

Remove the center screw and flat washer from the underside of the instrument

6. Carefully slide the instrument so that 2 of the 4 feet are hanging off the bench surface
and the screws can be accessed with a screwdriver.
Caution: Have someone hold the instrument so that is does not fall!
7. Remove the 2 feet by removing the screws holding them to the instrument.

Remove the 2 feet by removing the screws holding them to the instrument

8. Rotate the instrument so that the remaining 2 feet are hanging off the bench surface
and the screws can be accessed with a screwdriver.
9. Remove the last 2 feet by removing the screws holding them to the instrument.

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10. Slide the instrument back onto the bench.
11. Check the power supply and free it from the Base by gently pulling the rear of the Base
away from the supply.

Make sure the power supply will separate from the Base by gently pulling the rear of
the Base away from the supply.

12. The Base and Chassis are now ready to be separated. Firmly grip the Probe X rail with
both hands and lift the Chassis up and away from the Base. Set the Chassis down
carefully on a stable surface.

Lift Chassis up and out of Base Base with Chassis removed Power supply
opening

13. To re-install the Base, work in reverse order of above. Start by making sure the Base is
sitting on a stable surface and the Chassis has all the wiring secured on the underside
of the Chassis. There is minimal clearance between the Base and the Chassis so there
is no room for thick wire. There are 7 round spacers in the Base that must line up with
the 4 feet, 2 Photometer mounting screws and 1 center screw.
14. Use both hands to lift the Chassis by the Probe X rail bracket and carefully set the
Chassis back into the Base. Use the power supply opening as a reference for the
correct position. The 2 Photometer mounting screws will fall inside the 2 round spacers
in the Base limiting the movement.

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Looking at bottom side of Chassis the Looking at inside of Base
2 Photometer mounting screws are shown Photometer mounting screws fall
inside of these 2 round spacers

15. If the power supply was not disturbed while out of the Base, the thumb screw should be
re-installed. This will help to hold the Chassis in place while re-installing the feet and
center screw. If the power supply was moved or replaced, the strap that secures it
should be left loose so that the supply can be moved front to rear. It will need to be
adjusted to the Base after the feet are tightened.

Power supply strap screws

16. The drain manifold may also need to be adjusted if it was removed or moved. The
drain manifold has slots under the mounting screw so that it can be adjusted to be tight
against the Base after the feet have been tightened.

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Drain Manifold adjustment screws

17. Slide the instrument so that the front is hanging over the edge of the bench enough to
get two of the feet loosely re-installed. Do not fully tighten feet at this time.
18. Rotate the instrument so that the rear is now hanging over the edge of the bench
enough to get the remaining two feet loosely installed.
19. Once all four feet are loosely installed, carefully tilt the instrument on its back side to
loosely re-install the center screw and large washer.
20. With the instrument still tilted on its back edge, visually inspect the two Photometer
mounting screws. They should be as far towards the rear of the instrument as possible.
Once this position is confirmed, the center screw and the four feet can be tightened and
the instrument returned to its normal position on the bench.

Visually inspect the two Photometer mounting screws. They should be as far
towards the rear of the instrument as possible.

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21. Re-install the switch with the “O” facing down. Re-connect the switch connector to the
fuse board.
22. Re-install the tee and drain tubing.

3.1.7 Troubleshooting Guide for Enclosure

Problem: Possible Cause: Possible Solution:


Check components for correct
Cover not fitting Component not installed correctly installation
Base Inspect all wiring close to
Wire or tubing being trapped in
perimeter of base.
seam

Missing support post Install the support post


Cover screws not Stripped thread in support post Install longer screws in support
tight post

Support post loose Tighten support post


Prime bottle fitting Prime bottle block assembly moved Readjust prime bottle block
assembly

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3.2 Photometer
PHOTOMETER – this section contains Service Instructions, Troubleshooting Guide, Error
Messages and Command List
The Photometer measures the light path in the cuvette. The cuvette is positioned in front of the
aperture hole. The lamp comes already aligned as an assembly, and should be replaced as an
assembly. WARNING! The lamp is designed to be changed as an assembly; it is not
recommended that the technician try and replace just the lamp. The filters are good for many
years of service, however they many need to be replaced or cleaned. There is a replaceable
o-ring belt that drives the filter wheel, which can stretch or wear out over time. When changing
the lamp assembly, note that the push nut is there to prevent the thumbnut from falling off. The
push nut stops the thumbnut before it is completely unscrewed and falls off.
Service: The Photometer is designed to give years of trouble free service. There are a few
items that may need attention or replacement during the life of the instrument. They are:
1. Motor photometer (part #994089SP)
2. O-ring photometer (part #132059SP)
3. Photometer Board (part #995574SP)
4. Photometer Lamp (part #994615SP)
5. Thumbnut, Photometer (part #189621SP)
6. Push nut, Photometer (part #125100SP)
Each item is given step by step instructions below.
Push nut Thumbnut

Photometer Lamp Assembly Motor

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Photometer Board

O-ring belt
NOTE: SEE FILTER WHEEL SECTION FOR REPAIR OR REPLACEMENT OF FILTERS.

Service of Photometer:

3.2.1 Motor, Photometer (part #994089SP)

1. Remove the cover (see Section 3 for enclosure removal instructions). Remove the
Photometer from the chassis (reference Section 4).
2. With the photometer removed from the Chassis, remove the four screws that hold the
cover on the photometer with a Phillips screwdriver.

Remove 4 Phillips screws and take off the cover.

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3. Remove the four screws that hold the Photometer Board on and then remove the Board.
CAUTION: A grounding strap should be worn to avoid static discharge to the
Photometer PCB
Note: Do not touch the components on the PCB especially the photo diodes and their
windows. Place the PCB into an approved static bag for protection while working on the
O-ring.

Remove the 4 screws that hold the Photometer PCB in place.

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4. Remove the drive belt from the filter wheel.

Note: Do not let the filters get dirty, dusty, or contaminated.

Remove belt with your finger.


Two screws hold the motor.

5. Remove the two screws that hold the motor in place with a Phillips screwdriver.

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6. Unplug the motor connector and remove the motor.

Motor connector
7. Replace the motor. Re-assembly is reverse of disassembly.
8. Check the filter wheel speed. Filter wheel speed is monitored in milli-seconds per
revolution. The higher the number the slower the speed. Use command prompt and type
command FWMSPD. The filter wheel specification is 310 +/-10. If the speed needs to
change, type in command PTLIST and note value for HG3, FWMotorDuty. If the speed
needs to increase, then increase the value. Enter command FWMSPD ##, changing the
number to a higher value. Verify that the speed is correctly set at 310.

3.2.2 O-ring, Photometer (part #132059SP)

1. Remove the cover (see Enclosure section for detailed instructions). Remove the
Photometer from the chassis (see Section 4 for detailed instructions).

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2. With the photometer removed from the Chassis, remove the four screws that hold the
cover on the photometer with a Phillips screwdriver.

Remove 4 Phillips screws and take off the cover.

3. Remove the four screws that hold the Photometer Board on and then remove the Board.

CAUTION: A grounding strap should be worn to avoid static discharge to the


Photometer PCB

Note: Do not touch the components on the PCB especially the photo diodes and their
windows. Place the PCB into an approved static bag for protection while working on the
O-ring.

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Remove the 4 screws that hold the Photometer PCB in place.

4. Remove the drive belt from the filter wheel. .

Note: Do not let the filters get dirty, dusty, or contaminated.

Remove belt with your finger.

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5. Replace the belt with the new belt. Make sure the belt is centered on the filter wheel and
is not twisted. Re-assembly is the reverse order of disassembly.
6. Check the filter wheel speed. Use command prompt and type command FWMSPD. The
filter wheel specification is 310 +/-10. If the speed needs to change, type in command
PTLIST and note value for HG3, FWMotorDuty. If the speed needs to increase, then
increase the value. Enter command FWMSPD ##, changing the number to a higher
value. Verify that the speed is correctly set at 310.

3.3.3 Photometer Board (part #995574SP)

1. Remove the cover (see Enclosure section for detailed instructions). Remove the
Photometer from the chassis (see Section 4 for detailed instructions).
2. With the photometer removed from the Chassis, remove the four screws that hold the
cover on the photometer with a Phillips screwdriver.

Remove 4 Phillips screws and take off the cover.

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3. Remove the four screws that hold the Photometer Board on and then remove the Board.
CAUTION: A grounding strap should be worn to avoid static discharge to the
Photometer PCB
Note: Do not touch the components on the PCB especially the photo diodes and their
windows. Make sure the PCB stays in the static bag until you have dis-assembled everything
and are ready to replace the PCB.

Remove the 4 screws that hold the Photometer PCB in place.

4. Replace the Photometer Board. The re-assembly is the reverse order of disassembly.
5. Under pull down menus, go to “utilities”, “filter voltages” and a dialog box will appear.
Stage 1 is default, check to make sure voltages are good. This checks PTVOLTS, to
see if they fall between 2 to 10 volts. If they are, pull down the Stage 2 menu and
check filter voltages. This checks RVOLTS, to see if they fall between 3 to 8 volts. If
either is in the no good range, open up the communication window and send the
command PTEQ, which will equalize the ampl/offsets. Close the communication
window and recheck Stage 1 and Stage 2 Filter Voltages.

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3.2.4 Photometer Lamp (part #994615SP)
1. Make sure power is turned off.
2. Remove top enclosure of unit by removing the (3) thumbscrews.
thumb screws

3. Close shield so that it is covering the front of unit, exposing the rear of unit.

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4. Remove light shield by loosening thumbscrew to expose lamp bracket assembly.

THUMB SCREW

Push nut stops the blue thumbnut from unscrewing and falling off

Electrical connector Blue thumbnut

Wear gloves to prevent oil or dirt deposits from hands from getting on
glass bulb surface.

5. Disconnect yellow wires at electrical connector. Loosen the blue thumbnut - do not
take the thumbnut off the screw - there is a push nut to prevent this from happening.
Remove the lamp assembly.
6. Install the new lamp assembly. Guide and place the lamp assembly onto two locating
pins. (Wear protective gloves to prevent oils and dirt from getting on lamp bulb.) Make
sure the blue thumb nut is secured tightly upon re-assembly.
7. Reassemble in the reverse order of disassembly.

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8. Verify Filter volts are in correct range:
a. Power up unit
b. Observe photometer light. Light should be centered with lens.
c. Open the ChemWell®-T Manager
d. Go to Management from tool bar and Connect
e. Attach the power cord and turn the power switch on. Under pull down menus, go to
“Utilities”, “Filter voltages” and a dialog box will appear. Stage 1 is default, check to
make sure voltages are good. This checks PTVOLTS, to see if they fall between 2
to 10 volts. If they are, pull down the Stage 2 menu and check filter voltages. This
checks RVOLTS, to see if they fall between 3 to 8 volts. If either is in the no good
range, open up the communication window and send the command PTEQ, which
will equalize the ampl/offsets. Close the communication window and recheck Stage
1 and Stage 2 Filter Voltages to make sure they are in the good range.

3.2.5 Push nut, Photometer (part #125100SP)

1. Remove Top and lamp shield.

2. Take the new push nut and press it on to the end of the shaft with your finger.

Push nut installed on shaft

Step 3

3. Reinstall lamp shield and Top.

3.2.6 Thumbnut, Photometer (part #189621SP)

1. Remove Top and lamp shield.

2. Take a flat screw driver and pry off the push nut. Take care not to bend or break the
push nut.

Pry the push nut off the shaft with a flat screwdriver

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3. Remove the blue thumb nut. Install the new thumb nut. Install the push nut by pushing
back onto the shaft with your fingers.

4. Reinstall lamp shield and Top.

3.2.7 Troubleshooting Guide for Photometer

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Filter wheel does Power Not Turned Turn power on or check Power Supply to
not spin On instrument.
Check that heartbeat light is on.

Belt broken Replace the belt

Motor failure Change the motor.

Filter Wheel speed Belt has stretched Check the filter wheel speed and adjust if
it is not in specification.

Lamp does not turn Lamp burned out Change the lamp.
on.
Connector Make sure connector is securely plugged
unplugged. in.

Power supply faulty Check for correct voltage at the


connector.

Photometer volts Dirty or damaged Equalize the photometer volts.


out of range. filter Clean the filter or change the filter.
Dirty Lens Clean lens with lint free cloth.
Lamp Replace lamp.

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3.2.8 Photometer Command List

Name Description Explanation Syntax


List unit filters Displays a list of filters.
GETFILT GETFILT
0=340 1=405 2=505 3=545 Example 0 position = 340nm filter
4=580 5=630 6=999 7=999
Show Gain x1 volts Displays a gain x1 volts for each filter position.
0)5.0015 1)6.4485 2)5.2411
PTVOLTS 3)6.6575 4)5.8995 5)6.1320
PTVOLTS
Example 0 position = 5.0015 volts
6)0.0011 7)0.0014

Photometer volts Diplays equalized volts for each filter position.


RVOLTS 0)7.2800 1)7.2606 2)7.3448 Example 0 position = 7.2800 RVOLTS
3)7.4665 4)7.2944 5) 7.3561
6)0.0011 7)0.0013
LPON Lamp on. Turns the lamp on. LPON
LPOFF Lamp off. Turns the lamp off. LPOFF
FWMON Filter wheel motor on. Turns the filter wheel motor on. FWMON
FWMOFF Filter wheel motor off. Turns the filter wheel motor off. FWMOFF
Filter wheel motor speed. Displays the current filter wheel speed.
FWMSPD If the speed has just been changed, enter FWMSPD
old speed = 301 command again
PSTAT Photometer status Photometer status, either I (on) or o (off) PSTAT
Command needs 3 arguments, warmup, max
Set lamp group idle, cont
PSETLG Warmup time in seconds, max idle in seconds, cont
PSETLG N,N,N
in seconds
Lists all of the values for the different set
PTDUMP Photometer dump settings groups. PTDUMP
Automatically equalizes the amplitude and
PTEQ Equalize ampl/offsets offsets. PTEQ
Displays the current settings of the
PTLIST Photometer list settings photometer from all groups: Filter, Hardware, PTLIST
Waves, Amplitude, Offsets, Tune
PTSAVE Photometer save settings Saves the photometer settings. PTSAVE
Sets Photometer wave The first 6 filters are set at the
group factory, when adding new filter, use PTSETWG
PTSETWG
position 6 and then position 7. Zero X,X,X,X,X,X,X,X
becomes a placeholder.
Motor set hardware group Sets the motor hardware group.
8=Install wheel size [HG1] To increase speed change HG3 to a lower PTSETHG
PTSETHG 310 = Target Speed [HG2] number. Example PTSETHG 0,0,60 is the
62 = FWMotor Duty [HG3] command used to change the current setting N,N,N
to a faster wheel speed.

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3.2.9 Photometer Error Messages

*003: Primary Filter >{filter}<, not found

The primary filter specified for the assay is not mounted in the filter wheel.

*003: Differential Filter {filter}, not found request filter was not found

The differential filter specified for the assay is not mounted in the filter wheel.

*101: Photometer Timeout

Photometer did not respond after several tries.

*102: No Read: Mechanical error Read command was cancelled due to mechanism
issue

The photometer did not attempt to read, because of various mechanical issues
with the Shuttle mechanism.

*103: Photometer error

Photometer returned bad status.

*111: Filter voltage out of range

Photometer reports filter voltages outside of specifications.

*112: Get Volts failed

Photometer did not return voltage values during query.

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3.3 Filter Wheel
3.3.1 Filter Label
The filter label located on the lamp bracket describes the specific filter wheel configuration
for your particular instrument.
Filter wheel position is the physical placement of the filter on the wheel. The filter wheel
position numbers are shown below.

Standard configuration for the ChemWell®-T Filter Wheel


Position 1 – 340 (No Neutrals)
Position 2 – Blank
Position 3 – Blank
Position 4 – 630 (Neutrals 90, 30, 70)
Position 5 – 580 (Neutrals 90, 15, 70)
Position 6 – 545 (Neutrals 90, 30,70)
Position 7 – 505 (Neutrals 90, 50, 50, 70)
Position 8 – 405 (Neutrals 15, 50)

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3.3.2 Photometer Filter Replacement / Addition of New Filter

Materials/tools required:
• Replacement filter set or new filters to be added.
• 2- Phillips screw driver #1 and #2
• 1- Flat blade screw driver
• 1- retaining ring pliers

Photometer needs to be removed from the chassis to service the Filter Wheel.

1. Remove the cover (see Enclosure section for detailed instructions). Remove the
Photometer from the chassis, see Section 4.

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Photometer Filter Replacement / Addition of New Filter (Continued)

2. With the photometer removed from the Chassis, remove the four screws that hold the
cover on the photometer with a Phillips screwdriver.

Remove 4 Phillips screws and take off the cover.

3. Remove the four screws that hold the Photometer Board on and then remove the
Board.
CAUTION: A grounding strap should be worn to avoid static discharge to the
Photometer PCB

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Photometer Filter Replacement / Addition of New Filter (Continued)

Note: Do not touch the components on the PCB especially the photo diodes and their
windows. Place the PCB into an approved static bag for protection while work in being
done on the Filter Wheel.

Remove the 4 screws that hold the Photometer PCB in place.

4. Remove the drive belt from the filter wheel. Using the flat blade screwdriver, loosen the
filter wheel screw. Remove the wheel by pulling the screw and wheel together, away
from the bracket. Take care not to misplace the nylon washers. There are two nylon
washers, one gets installed on each side of the filter wheel.
Note: Do not let the filters get dirty, dusty, or contaminated. Note the orientation and
position and in the case of filters, the wavelength of anything removed.

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Photometer Filter Replacement / Addition of New Filter (Continued)

Note location of home hole. #1 position is 340nm at 1 o’clock


#2 and #3 are blank

Remove belt with your finger. Remove the screw with a flat screwdriver.
Filter wheel can then be removed.
NOTE: The filter position is numbered mechanically with Position 1 being at 1 o’clock.
However, the filter wheel rotates clockwise. This means as it reads:

Position # Physical location Filter #


Position #1 is equal to 0 (zero) which is filter 340nm on the PLIST
Position #8 is equal to 1 (one) which is filter 405nm on the PLIST
Position #7 is equal to 2 (two) which is filter 505nm on the PLIST
Position #6 is equal to 3 (three) which is filter 545nm on the PLIST
Position #5 is equal to 4 (four) which is filter 580nm on the PLIST
Position #4 is equal to 5 (five) which is filter 630nm on the PLIST

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Photometer Filter Replacement / Addition of New Filter (Continued)

5. Refer to Figure below. Locate the filter to be removed. The filters are fixed by use of a
retaining ring. Use the retaining ring pliers to remove the retaining ring. Pull the ring
away from the filter until the ring is dislodged and remove the ring and filter. The filter
may be bumped or pushed from the wheel using the eraser end of a pencil or other
blunt object. Remove any remaining filter components from the filter cavity.
6. Locate then install the neutral filters and/or screens if any that were included with the
replacement filter (some wavelengths do not require these extra screens) and place
them into the wheel cavity first. Next insert the filter with the flat ring side down. Place
the snap ring over the filter and use the retaining ring pliers to insert the retaining ring in
place.

NOTE: Make sure the filter does not move back and forth, sometimes the ring
does not get seated all the way down.

Retaining Ring Pliers Part No. 131135SP

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Photometer Filter Replacement / Addition of New Filter (Continued)

Neutrals must go between filter and filter wheel.

Home Position Hole

This diagram shows position 8, 405 Filter with 2 neutrals installed – a 15 and a 50
neutral. Some filters require neutrals, some others do not. Please see chart.

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Photometer Filter Replacement / Addition of New Filter (Continued)

6. Installation of the photometer is the reverse of disassembly. Reinstall the filter wheel on
the bracket taking care not to pinch any washer under the shaft. Check the lube to see
if it is dry, dirty, or missing. Add more lube if needed, on the screw shaft and on the
nylon washers. Tighten the shaft with the flat blade screwdriver. Be sure that the belt is
centered on the filter wheel and is not twisted. Install the photometer PCB taking care
to center the large hole around the shaft.
Note: Replace the cover taking care to fit and position the cover so that no light will
leak into the covered area.

7. Install the photometer onto the chassis.

8. Replace the Enclosure Cover.

9. Attach the power cord and turn the power switch on. Under pull down menus, go to
“utilities”, “filter voltages” and a dialog box will appear. Stage 1 is default, check to
make sure voltages are good. This checks PTVOLTS, to see if they fall between 2 to
10 volts. If they are, pull down the Stage 2 menu and check filter voltages. This checks
RVOLTS, to see if they fall between 3 to 8 volts. If either is in the no good range, open
up the communication window and send the command PTEQ, which will equalize the
ampl/offsets. Close the communication window and recheck Stage 1 and Stage 2 Filter
Voltages to make sure they are in the good range.

10. If a filter has been added to Position 6, type in the command:

PTSETWG 0 0 0 0 0 0 700 0

where there are 8 filter arguments and the first 6 (Position 0 through 5) are set up and
used as factory filters. Use 0 (zero) as a place holder, type in the filter wave in Position
6 and another 0 at the last position as a place holder. Type in PLIST to see if the
additional filter wave has been added, it will be displayed at the bottom line. Type in
PTSAVE. This example has added 700nm filter to Position 6.

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3.4 Cuvette Mover
CUVETTE MOVER – this section contains Service Instructions, Troubleshooting Guide, Error
Messages and Command List.

The Cuvette Mover transfers the Cuvette Carrier front to back, insuring proper positioning of
the Cuvette Carrier for the cuvette to be transferred into the photometer during testing. The
visible black surface under the Cuvette Carrier is known as the Cuvette Deck. On the surface
of the deck are three pins protruding out of a slot, these pins serve two purposes, to mate with
the bottom surface of the cuvette carrier to insure that it can only be installed in the proper
alignment and to drive the cuvette carrier along the cuvette deck. The Cuvette Deck also acts
as the heating surface for the reactions in the cuvette.

Service: The Cuvette Mover is designed to give years of trouble free service. There are a few
items that may need attention or replacement during the life of the instrument. They are:

1. Motor (part #994638SP)


2. O-Ring Belt Cuvette (part #132060SP)
3. Encoder Board (part #995573SP)
4. V Mover Motor Controller Board (part #994632SP)
5. Thermistor (part #995564SP)
6. Opto Right Angle (part #995571SP)
7. Cuvette Cover (part #994626SP)

Each item is given step by step instructions below. Reference Section 4 Removal of Sub-
assemblies from the Chassis.

CUVETTE DECK MOTOR

THERMISTOR

O-RING BELT

CUVETTE MOVER ASSEMBLY


OPTO RIGHT ANGLE
ENCODER BOARD

CUVETTE DECK

V MOVER MOTOR CONTROLLER BOARD

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3.4.1 Motor (part #994638SP)

1. Make sure that the power is turned off.

2. Remove the cover. Open product enclosure as described in the Enclosure Section of the
service manual.

3. Disconnect the resistors, thermistor, and motor controls coming from Cuvette Carrier at
the main Circuit board. Disconnect all ground wires from the Cuvette Carrier as well.

2 yellow connectors motor connector 3 connectors

4. Remove four mounting screws on top of the Cuvette Deck, two flat heads and two pan
head screws, with a Phillips head screwdriver.

Mounting Screws

5. Remove the Cuvette Deck by pulling it toward the front of the product, turning it slightly
from side to side as you remove it may help. Insure that no wires are hung up.

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6. Remove Encoder from Motor Mount be removing 2 screws with a Phillips head screw
driver.

ENCODER BOARD
MOUNTING SCREWS

7. Remove the belt from between the two pulleys by the motor.

O-RING BELT

8. Remove pulley from motor shaft by pulling it straight off.

MOTOR PULLEY

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9. Remove motor by removing two Phillips head screws.

MOUNTING SCREWS

10. Install new motor using the same two Phillips head screws previously used.

11. Reinstall the motor pulley; insure that it is lined up with the other pulley while looking
from the side.

PROPER PULLEY ALIGNMENT

12. Reinstall belt onto pulleys.

13. Reinstall encoder; make sure encoder wheel attached to the lead screw pulley is not
contacting the encoder board.

14. Place Cuvette Deck back onto its mounts, make sure that all wires are routed properly
and are will not be pinched when the Cuvette Deck is secured back down.

15. Connect all wire to their proper locations. Secure the Cuvette deck back in place.
Reinstall product covers as described on Enclosure section of manual.

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3.4.2 O-Ring Belt Cuvette (part #132060SP)

1. Make sure that the power is turned off.

2. Remove the cover. Open product enclosure as described in the Enclosure Section of
the service manual.

3. Disconnect the resistors, thermistor, and motor controls coming from Cuvette Carrier at
the main Circuit board. Disconnect all ground wires from the Cuvette Carrier as well.

2 yellow connectors motor connector 3 connectors

4. Remove four mounting screws on top of the Cuvette Deck, two flat head screws and
two pan head screws, with a Phillips head screwdriver.

Mounting Screws

5. Remove the Cuvette Deck by pulling it toward the front of the product, turning it slightly
from side to side as you remove it may help. Insure that no wires are hung up.

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6. Remove Encoder from Motor Mount be removing 2 screws with Phillips head screw
driver.

ENCODER MOUNTING SCREWS

7. Remove and replace the belt from between the two pulleys by the motor.

O-RING BELT

8. Reinstall Encoder make sure encoder wheel attached to the lead screw pulley is not
contacting the encoder board.

9. Place Cuvette Deck back onto its mounts, make sure that all wires are routed properly
and are will not be pinched when the Cuvette Deck is secured back down.

10. Connect all wire to their proper locations.

11. Connect all wire to their proper locations. Secure the Cuvette deck back in place.
Reinstall product covers as described on Enclosure section of manual.

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3.4.3 Encoder Board (part #995573SP)

1. Make sure that the power is turned off

2. Remove the cover. Open product enclosure as described in the Enclosure Section of
the service manual.

3. Disconnect the resistors, thermistor, and motor controls coming from Cuvette Carrier at
the main circuit board. Disconnect all ground wires from the Cuvette Carrier as well.
Note: Board must come out to remove cables or unit must be removed from base
enclosure.

2 yellow connectors motor connector 3 connectors

4. Remove four mounting screws on top of the Cuvette Deck, two flat head screws and
two pan head screws, with a Phillips head screwdriver.

Mounting Screws

5. Remove the Cuvette Deck by pulling it toward the front of the product, turning it slightly
from side to side as you remove it may help. Insure that no wires are hung up.

6. Unplug four-pin connector from encoder board.

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Encoder Board (Continued)

7. Remove old encoder, and mounting bracket from motor mount be removing 2 screws
with a Phillips head screw driver.

MOUNTING SCREWS

ENCODER

8. Remove encoder board from its mounting bracket. Keep mounting bracket, nylon
washers, and screws.

9. Install new encoder board on to mounting bracket with nylon washers in-between the
board and mounting bracket.

Screws

Encoder Board

Nylon Washers

Mounting Bracket

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Encoder Board (Continued)

Encoder and Encoder Wheel Clearance

10. Reinstall mounted encoder on motor bracket and reconnect four-pin connector, make
sure encoder wheel attached to the lead screw pulley is not contacting the encoder
board.

11. Place Cuvette Deck back onto its mounts, make sure that all wires are routed properly
and are will not be pinched when the Cuvette Deck is secured back down.

12. Connect all wire to their proper locations.

13. Connect all wire to their proper locations. Secure the Cuvette deck back in place.
Reinstall product covers as described on Enclosure section of manual.

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3.4.4 V Mover Motor Controller Board (part #994632SP)

1. Make sure that the power is turned off.

2. Remove the cover. Follow instructions for opening enclosure from Enclosure Section of
Service Manual.

3. Disconnect all wires from V Mover Control Board. NOTE: Can not disconnect wires
from board without removing board.

2 yellow connectors motor connector 3 connectors

3 connectors

2 connectors

4. Remove Screws on board mounting bracket.

Mounting Screws

5. Remove V Mover Control Board and Mounting Bracket.

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6. Reconnect all wire to their proper locations

7. Install V Mover Control Board with Mounting Bracket, reusing hardware.

8. Reinstall product covers as described on Enclosure section of manual.

3.4.5 Thermistor (part #995564TSP)

1. Make sure that the power is turned off.

2. Open product enclosure as described in the Enclosure Section of the service manual.

3. Disconnect the resistors, thermistor, and motor controls coming from Cuvette Carrier at
the main Circuit board. Disconnect all ground wires from the Cuvette Carrier.

2 yellow connectors motor connector 3 connectors

4. Remove four mounting screws on top of the Cuvette Deck, two flat head screws and
two pan head screws, with a Phillips head screwdriver.

Mounting Screws

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Thermistor (Continued)

5. Remove the Cuvette Deck by pulling it toward the front of the product, turning it slightly
side to side as you remove it may help. Insure that no wires are hung up.

6. Remove Thermistor mounting screw, remove and disregard the Thermistor, keep the
aluminum block, mounting screw and nylon washers. Tie wraps will need to be cut off
and removed.

Mounting Screw

7. Install new Thermistor board onto cuvette deck with aluminum block contacting the
Cuvette Deck, apply thermal grease between the block and the cuvette deck, place a
nylon washer between both the Thermistors and board, and the Thermistor and screw
head.

Thermistor
Board

Screw Head
Mounting Block

Nylon Washers

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Thermistor (Continued)

8. Place Cuvette Deck back onto its mounts, make sure that all wires are routed properly
and are will not be pinched when the Cuvette Deck is secured back down.

9. Connect all wire to their proper locations. Secure the Cuvette deck back in place.
Reinstall product covers as described on Enclosure section of manual.

3.4.6 Opto Right Angle (part #995571SP)

1. Make sure that the power is turned off.

2. Remove the cover. Open product enclosure as described in the Enclosure Section of
the service manual.

3. Disconnect the resistors, thermistor, and motor controls coming from Cuvette Carrier at
the main Circuit board. Disconnect all ground wires from the Cuvette Carrier.

2 yellow connectors motor connector 3 connectors

4. Remove four mounting screws on top of the Cuvette Deck, two flat head screws and
two pan head screws, with a Phillips head screwdriver.

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Mounting Screws

5. Remove the Cuvette Deck by pulling it toward the front of the product, turning it slightly
side to side as you remove it may help. Insure that no wires are hung up.

6. Remove Motor Guide Bracket. By removing two mounting screws. Unplug 4-pin
connectors from top of Opto Right Angle.

Opto Right Angle

Mounting Screws

7. Remove mounting screw in the center of the Opto Right Angle, and replace with new
part and reinstall mounting screw.

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Mounting Screw

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8. Reconnect 4-pin connectors and reinstall Motor guide Bracket.

9. Connect all wire to their proper locations. Secure the Cuvette deck back in place.
Reinstall product covers as described on Enclosure section of manual.

3.4.7 Cuvette Cover (part #994626SP)

1. Install the new Cuvette Cover.

3.4.8 Troubleshooting Guide for Cuvette Mover


PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION
Cuvette Mover Power Not Turned On Turn power on or check Power Supply to
Does Not Move instrument.
Check that heartbeat light is on.

USB Cable Not Connected Connect USB cable and restart software
Use Communication window to check status
Use reconnect command

Mechanical Jam
Check that encoder wheel turns freely and is
not jammed in home position.
Check that lead screw is not jammed and
operates freely.

Electrical Problem Check motor is getting power.


Check all connectors on Motor Controller
board.
Cuvette Mover Mechanical Jam
Jam Message Check that encoder wheel turns freely and is
not jammed in home position.
Check that lead screw is not jammed and
operates freely.

Electrical Problem Check that motor is getting power.


Check connectors on motor controller board.

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3.4.9 Cuvette Mover Command List
Note for Command Prompt: Cuvette Mover = VRack and Shuttle = VShutt

Name Description Explanation Syntax


HEATOFF Turns reaction heating off Used to turn heating off HEATOFF
HEATON Turns reaction heating on Used to turn heating on HEATON
Status display of heat temp Status of reaction heater HEATTEMP
data Reaction Heater is on Reaction temp setting HG1,
HEATTEMP Heat temp [37.0] 36.7 (-1.0) measured temp, reaction
[693] trim temp, raw counts from
analog
Reaction heat adjusting Used at factory to calibrate HEATTRIM
HEATTRIM
value, HG2 heating
Reaction temperature value. A value (n) is required. HEATSET n
HEATSET
Typically set to 37.0
Homes Shuttle and Cuvette Used to home the Cuvette VHOME
VHOME
Mover movers
A value (n) in inches is
VRKM Cuvette Moves to a distance required, decimal point not
in inches used. VRKM n
Exercises Cuvette Mover and Used to check for VTIM
VTIM displays time mechanical problems
V time = 19.8 sec
Status display of Cuvette Used when the status info VSTAT
VSTAT
Mover and Shuttle is needed.
Vrack pos=2 Lastdest=0,
state = G[0]0
Vshutt pos=1,Lastdes=
-3,state=G[0]0

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3.4.10 Cuvette Mover Error Messages

*002: VRack command exceeds allowed range at {CurPos} of >{ReqPos}<

Position sent to mover exceeds maximum range specified at the mover module.

*002: VRack stat timeout at ?? of >{ReqPos}<

Mover module did not respond after a few tries.

*002: VRack break cmd recvd at {CurPos} of >{ReqPos}<

Mover module responded with a interrupt indication.

*009: Vrack is timing out (10 tries)

PRV module did not respond.

*009: Vrack has mech jam at {CurPos} of {LastDest} req->{ReqPos}

VRack mover module is reporting a stuck condition.

*009: Vrack has mech jam (long running) at {CurPos} of {LastDest} req-
>{ReqPos}

VRack mover module has been running and still not reached destination.

*009: VRack has mech jam (EndLimit) at {CurPos} of {LastDest} req->{ReqPos}

VRack mover module report mechanism has reached the limit sensor

*015: VRack move timeout at ?? of >ReqPos}<

VRack mover issued bad status

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3.5 Diluter
DILUTER – this section contains Service Instructions, Troubleshooting Guide, Error Messages
and Command List

The Diluter aspirates and dispenses controlled amounts of reagents and samples to the
reaction. Typical to most reactions, the precise amount dispensed is very important. Power
requirements are 24V dc to the stepper motor. The power supply delivers 12 volts, which is
then transformed to 24V dc. The 24VDC Supply board is a separate PCB mounted on the far
side of the Diluter. The 500µl syringe has an O-ring seal and plunger that can be replaced
separately or the complete syringe which includes the O-ring and plunger can be replaced.
Service to the syringe can be done without taking out the Diluter. Service to the valve, motor,
belt or PCB’s is most easily done by removing the Diluter from the chassis.

Service: The Diluter is designed to give years of trouble free service. There are a few items
that may need attention or replacement during the life of the instrument. They are:
1. Motor (part #105102SP-46)
2. Belt, small, Diluter (part #132048SP)
3. Valve, Diluter (part #105196SP)
4. Diluter Board (part #995575SP)
5. 24VDC Supply Board (part #995578SP)
6. Syringe Cover Diluter (part #189637SP)
7. Syringe, Diluter (part #105189SP)
8. O-ring & Plunger (part #046005)

Each item is given step by step instructions below.

To service the Motor, Belt, Valve, Diluter Board, or 24 VDC Supply Board it is recommended
that the Diluter be removed from the instrument. See Section 4, sub-assembly removal.

Valve Syringe O-ring & Plunger

24VDC Board Diluter Board Motor Belt

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3.5.1 Motor (part #105102SP-46)
NOTE: See Section 4 for Diluter subassembly removal.
1. To change the motor it will first be necessary to remove the sheet metal support
bracket. To remove this bracket, remove the 2 flat head screws in the diluter panel and
the 2 socket head screws that are fastened through the sheet metal bracket just below
the Motor drive pulley. Be careful not to lose the nylon spacers between the sheet
metal and the motor mounting plate.

Remove 2 Flat Head Screws Remove 2 Socket Head Screws

2. With the sheet metal support bracket removed, gently slide off the black drive belt and
loosen the set screw in the Motor drive pulley then pull the pulley off the motor shaft.

3. Unplug the motor connector from the Diluter PCB board and remove the 2 remaining
socket head screws.

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Remove Black Belt Loosen Motor drive pulley set screw Remove 2 socket head screws

4. The motor can now be removed from the Diluter. Install the new motor in the reverse
order. Make sure to set the motor drive pulley so that the screws do not rub on the
inside of the sheet metal support bracket.

3.5.2 Belt, small, Diluter (part #132048SP)


1. To install a new belt on the Diluter, the sheet metal support bracket will need to be
removed. See Section 1, #1 in the previous section for this procedure.

2. With the sheet metal support bracket removed, the black belt can be removed from the
pulleys and the new belt installed. There is no adjustment on this belt.

3. Re-install the sheet metal support bracket in the reverse order.

3.5.3 Valve, Diluter (part #105196SP)


1. To change the valve, it is recommended to remove the manifold from the Diluter. Start
by disconnecting the valve wire from the PCB board.

2. Unscrew the Syringe then remove the 2 screws from the top of the manifold.

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Unscrew Syringe Remove 2 screws from top of Manifold

3. Lift the manifold and valve up and away from the Diluter.
4. Remove the 2 small screws holding the valve to the bottom of the manifold.

Remove 2 screws holding valve to manifold

5. Pull the valve away from the manifold. Pay special attention to the small dowel pin
located in the manifold to match the hole in the valve. This dowel pin prevents the
valve from being installed incorrectly. Also note the small o-rings that make the seal
between the valve and the manifold. These must be seated in the valve before re-
assembly.

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Make sure small o-rings are seated on the valve before installing new valve!

6. Install new valve in reverse order of above being careful to line up the small dowel pin
correctly with the valve and the 3 small o-rings are seated on the valve. Finish
reassembly of manifold and Syringe and re-plug the valve into the PCB board.

3.5.4 Diluter Board (part #995575SP)


NOTE: See Section 4 for Diluter subassembly removal.
1. To replace the Diluter Board, start by disconnecting the motor and valve connectors.

2. Remove the 4 Pan Head screws that hold the 2 mounting brackets to the frame. This
will allow the Diluter Board and mounting brackets to be released from the Diluter.

Remove the 4 Pan Head screws

3. Remove the 3 screws that hold the Diluter Board to the mounting brackets. Make note
of the nylon spacers that are used to maintain the correct spacing. If the 24VDC
Supply Board is not being replaced, remove the screw and spacer that connect the 2
boards together. Separate the boards by carefully pulling on the connector that is
between them.

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Remove the 3 screws holding the Diluter Board to the mounting brackets

4. Attach the 24VDC Supply board to the new Diluter board and install the screw, spacer
and nut that holds them together.

Screw holding 24VDC board to Diluter Board 24VDC Board

5. Re-attach the mounting brackets to the new Diluter board using the same screws and
spacers that were removed from the old board.
6. Reattach the mounting brackets to the frame of the Diluter using the same pan head
screws that were removed.
7. Check the stroke of the Diluter to make sure the flag is positioned correctly between the
optical sensors. Normally the flag does not need to be readjusted. As a check, when
the unit is under power make sure the syringe plunger tip does not bottom out when it
dispenses. If it does, contact the factory for further instructions.
8. Re-connect the motor and valve connector to the board.

3.5.5 24VDC Supply Board (part #995578SP)

1. Changing the 24VDC Supply Board has been covered in the previous section. Please
see Section 3.5.4 for procedure.

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3.5.6 Syringe Cover Diluter (part #189637SP)

1. The Syringe Cover can easily be replaced by lifting the cover off the pins in the
manifold and replacing with a new Cover.

Pin holes Lift off pins and replace as needed

3.5.7 Syringe, Diluter (part #105189SP)

1. To replace the Syringe, loosen the thumb screw on the back end of the Syringe then
firmly grip the Syringe body and unscrew it from the Luer fitting on the manifold. Lift the
syringe off the Diluter drive arm. Screw the new Syringe on the Luer fitting in the
reverse manner then re-attach retighten the thumb screw.

3.5.8 O-ring & Plunger (part #046005SP)

1. To replace the O-ring and Plunger, begin by removing the plunger assembly from the
Syringe body.
2. Remove the white plunger by cutting with a sharp blade.
3. There is an o-ring under the plunger. Remove the old o-ring and replace with new o-
ring.
4. Install the new plunger onto the end of the plunger assembly and over the new o-ring
by placing plunger on a flat surface and then inserting plunger assembly.
5. Re-install the plunger assembly into the Syringe Body.

O-ring & Plunger Pull Plunger off end of rod

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3.5.9 Manifold (part #046007SP)

1. Changing the Manifold can be done by following the steps in Section 3.

2. Once the Manifold has been changed, it should be primed and checked for leaks
before performing any tests.

Manifold assembly

3.5.10 Removing Diluter from the Chassis


1. To remove the Diluter, the Enclosure Cover will need to be removed (see Section 4).

2. Unscrew the black fitting from the rear of the Diluter manifold.

3. Remove the 4 screws and ground terminal from the Diluter mounting brackets.

Remove the 4 screws and ground terminal from the Diluter mounting brackets

4. Lift the Diluter slightly and unplug the USB and power connectors from the PCB located
under the Diluter.

5. Lift Diluter out of instrument.

6. Re-install the Diluter in reverse order of above. Use care when plugging the
connectors back into the PCB as it is easy to plug them incorrectly.

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3.5.11 Diluter Troubleshooting Guide

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Air Bubbles Leak in tubing Check fittings for tightness.
connection Check flare for damage, re-flare if needed.

Plunger worn out Replace plunger and o-ring.

Diluter Cavitation Excessive restriction on probe side of


manifold.
Check for pinched tubing.
Clean inside of probe tip. Check manifold
for debris.

Damaged tubing Repair or replace tubing.

Air leak at plug Tighten or replace plug.

Syringe air leak Tighten syringe on Luer fitting.


Tighten or replace Luer fitting.

Syringe does Mechanical Jam Remove diluter from chassis and verify the
not move motor turns.

Motor does not move Check motor wires and connector.


Check 24VDC supply board.
Check connections on Diluter board and
main board.

Noise Motor drive pulley Check for clearance between the screws
hitting bracket. and the metal support bracket.

Syringe plunger Re-adjust home flag position.


hitting bottom of Check that Luer fitting is screwed in all the
syringe way.

Carriage noise Lubricate lead screw and guide rods with


oil.
Check lead nut for tightness.

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3.5.12 Diluter Command List

Name Description Explanation Syntax


Displays the status of the reserve, between 99-
BLPR Backlash push reserve 0. BLPR
Diluter prime Primes system a given number of times. DPRI n
DPRI
Example DPRI 5 will prime the system 5 times.
Diluter aspirate Requires volume # (in µl) and speed (1-9) DASP nn
DASP Example DASP 100 9 will aspirate 100µl speed n
9
Diluter dispense Requires volume # (in µl) and speed (1-9)
DDSP Example DDSP 100 7 will dispense 100µl DDSP nn
speed 7
Initializes to make sure everything is
DINI Diluter initialize working. DINI
DSAVE Diluter save settings. Saves diluter settings. DSAVE
Query diluter Query’s the diluter.
Query W =
QDIL >wEC01w028Dw< QDIL
Syr-0 653, Syr-1 60417 Displays position of syringe plunger
Query F = >V46 V1.3 < in counts and firmware version.
Diluter make air pocket Requires volume # (in µl) and speed (1-9) DMAP
DMAP Example DMAP 8 3 will make air pocket 8µl nn n
speed 3
DSETDEFAULT Diluter set to defaults Sets diluter to factory settings
Diluter list settings Requires a number after (1-5) DLIST n
Active Profile # 1 [315]
[500ul_HS] = EntryName
[MS1]
H = StepMode
[MS2]
2000 = RunLength_steps
[MS3]
0=
BackLashCorrectionAmtsteps
[MS4]
500 = SyringeSize_uL
[SS1]
DLIST 1 = AirGap_uL
[SS2]
Profiles can be active or inactive.
90 = PushVolReserve_uL Usually #1 profile is active.
[SS3]
9 = Wash Speed
[SS4]
9 = Air Gap Speed
[SS5]
0 = Syringe No
[SS6]
1 = Valve Polarity
[GP1]
Speed List:0: 800 1: 850 2:
900 3:1000 4:1200 5:1500
6:1600 7:1700 8:1800 9:2150
DLOAD Diluter load settings Moves settings from flash to main. DLOAD

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3.5.13 Diluter Error Messages

*010 Diluter command TimeOut

Diluter module did not respond to command

Possible Cause: Diluter not connected; board or motor issue

Possible Solution: Check Diluter connections; check boards and motor

*010 Diluter Not ready

Diluter module did not respond to status check

Possible Cause: Malfunction on board;

Possible Solution: Check boards and connections

*201 Attempted to aspirate more than syringe capacity

Diluter volume spec exceeded

Possible Cause: Corrupted or bad command

Possible Solution: Correct command; remain in specified volume range

*202 Attempted to dispense more than remaining in syringe

Diluter volume was exhausted

Possible Cause: Corrupted or bad command

Possible Solution: Correct command; remain in specified volume range

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3.6 Probe X
Probe X - this section contains Service Instructions, Troubleshooting Guide, Error Messages and
Command List

The Probe X axis has a two phase Encoder Wheel and matching sensor pair on the left side which
provides tachometer pulses to the microprocessor.

CAUTION: The encoder wheel is fragile, do not damage.


Use care in handling - wheel is sharp!

The two phases of the encoder output are used by the software to determine direction and
accurately track the probe’s position. On the right side of X Interface Board, there is a sensor with a
phototransistor and LED that is at a known fixed point and is used as a reference to verify the
encoder wheel count is correct and if not resets the count in software. An additional sensor to the
far left of the mechanism senses home for the microprocessor.

Service: The Probe X is designed to give years of trouble free service. There are a few items that
may need attention or replacement during the life of the instrument. They are:
1. Black Belt (part #132048SP)
2. Motor with Encoder (part #994618SP)
3. Yellow Belt (part #132049SP)
4. Probe X Interface Board (part #995569SP)
5. Motor Controller Board (part #994633SP) NOTE: See Rack Mover Section for
replacement of the Motor Controller Board.

Each item is given step by step instructions below.

Yellow Belt  Sensor    Probe X Interface Board    Motor with Encoder     Black Belt 

(Probe X shown with Probe Z removed) 

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Changing Service Parts:

It is recommended to remove the Shield and Top prior to servicing any Probe X component. NOTE:
See Enclosure section for removal procedure.

3.6.1 Black Belt (part #132048SP)


1. Remove the Yellow Belt from the Idler pulley by gently sliding the belt off the pulley
while slowly moving the Probe X.

Idler Bracket Pulley 

Gently pull Yellow belt off the Idler Pulley  

2. Follow the steps #4 through #6 in Section 1 to loosen the hex screws on the motor,
loosen the Black Belt and then reinstall in reverse order. It is not necessary to
disconnect the wiring or remove the Motor Cover. Adjust the belt as shown in Section 1.

3. Re-install the Yellow Belt by putting the left side around the drive pulley first, then slide
the yellow belt back on the Idler pulley in the reverse of Step 1.

4. Run the Probe X with Motor to check for smooth operation.

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3.6.2 Motor with Encoder (part #994618SP)
1. Disconnect the 2 ground wires from the spade connector on the back of the motor
cover.

2. Remove the 3 screws and ground terminal from the motor cover and remove cover.
Use caution when removing cover that the long nylon spacer does not get dislodged or
lost. This spacer is used to maintain the correct spacing from the Probe X Interface
board to the cover.

Long Nylon Spacer    3 Screws that hold the Motor Cover    Ground Terminal 

3. Disconnect the motor power and encoder connector from the Probe X Interface Board.
Release tension on the yellow belt or remove the yellow belt.

4. Use a 2.5mm hex wrench to remove the 2 hex screws that holds the motor to the Motor
mounting bracket. Once the screws are removed the motor can be released from the
Black belt and removed from the mounting bracket.

5. Replace or service Motor with Encoder as needed and reinstall in the reverse order.

6. Leave the 2 hex screws loose and adjust the Black belt to have a small amount of play
by sliding the motor and then tighten the hex screws. Caution must be used not to over
tighten the Black belt as this will put unwanted strain on the motor and could affect the
instrument performance. Too little tension could cause the Black belt to ride over the
pulley teeth and cause uneven movement of the Probe X. There should be
approximately 1mm of play at the center between the pulleys when a slight pressure is
applied with the fingertip.

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Adjust for 1mm of play

2 Hex Bolts that hold Motor to bracket 

7. Re-connect the motor and encoder connectors to the interface board.

8. Re-install the Motor cover and ground wires.

3.6.3 Yellow Belt (part #132049SP)


1. The Yellow Belt can be adjusted by loosening the 2 Hex Head screws located under
the Idler Bracket. Use a ¼” wrench to loosen the 2 screws, then apply additional
tension to the belt and re-tighten the screws. Caution should be used to over tighten
the yellow belt as this could cause poor performance of the Probe X. The belt should
be checked when the Probe X is home. It should be between 4 and 6mm when you
push from the top downward at the center distance between the pulleys.

CAUTION!
Do not over
tighten.

  Apply tension out to tighten 
yellow belt  

Loosen these 2 screws to adjust Probe X Yellow Belt 

2. To replace the Yellow belt it will be necessary to remove the Idler Bracket by removing
the 2 hex head screws that hold the Idler bracket. Remove the Yellow belt from the
Drive pulley. It is highly recommended that the Probe Tip be removed before
proceeding to the next step. See Probe Z Section.

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Yellow Belt (Continued)

3. Slide the Probe X and Probe Z out of the slide rail so that it is clear of the Probe X rail.

4. Allow the Probe X and Probe Z to turn upside down to gain access to the Yellow belt
attaching screw. Be careful not to damage the Igus flexible wire chain.

5. Pay special attention to the way the Yellow belt is attached and re-attach the new belt
in the same manner by removing the attaching screw and re-attaching the new belt.
Caution should be used to make sure the teeth on the new belt face the same way as
on the old belt and not to get the Yellow belt twisted.

6. Carefully turn the Probe X and Probe Z back over to normal condition and slide it back
into the rail in the opposite manner from step #3.

7. Check the Yellow belt is correctly installed by putting the left end over the drive pulley
and stretching it out to where the Idler pulley will be. If the belt looks correct, proceed
to the next step. If the belt does not look correct, repeat steps #3 through #7 until it
looks correct.

8. Re-attach the Idler bracket by re-installing the 2 hex head screws.

9. Place the yellow belt over the Idler pulley and set tension as described in Step #1 of
this Section.

10. Replace the enclosure and put the machine under power. Open the command window
and type in the command XTIM. The mechanism should run smoothly with no clicking.
A clicking .sound usually indicates the yellow belt is too loose and is skipping over a
tooth on the drive pulley.

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3.6.4 Probe X Interface Board (part #995569SP)
1. The Probe X interface Board has no user serviceable parts. It serves as the electrical
connection board for the Probe X and Probe Z. It has 2 optical sensors; one is a home
position and the other is the reference sensor. If the board becomes damaged and
needs to be changed, it will be necessary to remove the Motor Cover. Refer to Section
1, #2 for this procedure.

2. Disconnect all connectors from the Probe X interface Board.

3. Remove the 3 screws holding the interface Board to the Probe X bracket rail.

4. Make sure the 4 nylon spacers are attached to the bottom side of the new interface
board. (3 mounting screws and 1 under Motor cover screw)

5. Re-install the new board with the 3 screws that were removed from the old board.

6. Re-attach all electrical connectors.

7. Re-install the Motor Cover and ground wires.

8. From the command prompt type in XREF to verify unit is working. Open the Utilities
Menu and click on the Alignment tab. Three items need to be checked and realigned:
the Rack, Wash Cup, and Probe Vs. Cuvette. After alignment, open the command
window and check the XTIM and verify that it moves within the specified time limits.

Spacers under the interface Board (4)      3 Screws that hold the interface board 

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3.6.5 Probe X Troubleshooting

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Probe X does Power not turned on Check for power on the instrument
not move Check heart beat light on main board.

USB cable not Check status in command window. Replace


connected cable.

Mechanical Jam Check that Probe X moves freely.


Lubricate rails with oil.
Adjust truck tension.

Broken belt Replace black or yellow belt.

Electrical problem Check motor is getting power


Check wiring from motor controller board to
interface board.

Probe X noise Belt skipping Adjust yellow belt.

Loose fastener Check for loose screws on Probe X

Felt pad Check felt pads, they should just be


touching rail.

Truck adjustment Contact Technical Support.

Probe X not Rails dry Clean and lubricate rails with oil.
running
smooth
Black belt Check black belt for wear, adjust black belt
tension.

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3.6.6 Probe X Error Messages

*002: {Probe X} command exceeds allowed range at {CurPos} of >{ReqPos}<

Position sent to mover exceeds maximum range specified at the mover module

Possible Cause: Corrupted command; malfunction of home or reference


sensor.

Possible Solution: Reboot software; restart instrument; check home and


reference sensors on the Probe X interface board for damage.

*002: {Probe X stat timeout at ?? of >{ReqPos}<

Mover module did not respond after a few tries

Possible Cause: Probe X did not move to requested destination in the expected
time frame.

Possible Solution: Check for Mechanical or Electrical issues. Reboot software,


instrument.

*002: {Probe X} break cmd recvd at {CurPos} of >{ReqPos}<

Mover module responded with an interrupt indication

Possible Cause: The Probe X was interrupted during the expected move.

Possible Solution: Check for mechanical sources that may have caused an
interrupt during the move. Clear area of source of interrupt.

*007: Probe X has mech jam at {CurPos} of {LastDest} req->{ReqPos}

Mover module is reporting a stuck condition

Possible Cause: Motor failure or incorrect Motor settings. Rails have become
dry or dirty creating excessive friction. Possible foreign material has jammed
the mechanism. Black belt has jumped a tooth and created a jam. Felt pads
have been adjusted to tight to the rail. Connector has become loose or no
current to motor.

Possible Solution: Check motor and settings. Clean and lubricate the slide rails.
Remove any foreign material that may be jamming mechanism. Inspect Black
belt for jamming and correct tension. Re-adjust trucks. Adjust felt pads so they
just touch lightly on the rail. Check connectors and wires for current to motor.

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Probe X Error Messages (Continued)

*007: Probe X has mech jam (long running) at {CurPos} of {LastDest} req-
>{ReqPos}

Mover module has been running and still not reached destination

Possible Cause: Motor failure or incorrect Motor settings. Rails have become
dry or dirty creating excessive friction. Possible foreign material is jamming
mechanism. Felt pads have been adjusted to tight to the rail.

Possible Solution: Check motor and settings. Clean and lubricate the slide
rails. Remove any foreign material that may b e jamming mechanism. Re-
adjust trucks. Adjust felt pads so they just touch lightly on the rail.

*007: Probe X has mech jam (EndLimit) at {CurPos} of {LastDest} req->{ReqPos}

Mover module report mechanism has reached the limit sensor

Possible Cause: Probe X has jammed at the home position or the flag has
traveled past home and continues to look for home by jamming all the way to
the left. The travel limiter bracket may have been removed or moved and the
instrument was turned on with the home flag past the sensor. Possible damage
to the home or reference sensor.

Possible Solution: Re-install the limiter bracket. Turn off instrument and move
the Probe X to the right side of the instrument. Turn the instrument back on so
that it will seek the home position at the left side. Inspect the home and
reference sensors for damage.

*015: Probe X move timeout at ?? of >ReqPos}<

Mover issued bad status

Possible Cause: Probe X corrupted command.

Possible Solution: Reboot software and restart instrument.

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3.6.7 Probe X Command List

Name Description Explanation Syntax


Moves the Probe X to the home
PRXH Probe X home position PRXH
Moves the Probe X to a position. PRXM
This value is displayed in inches. nnnn
PRXM Probe X to position
Example- to move the Probe X to
5.00" PRXM 500
Probe X current status Displays the current status of the
Probe X.
XSTAT ProbeX pos = 498 ProbeX position in inches 4.98 XSTAT
Lastdest = 500 Last destination requested in inches
state = G [0] 8903 5.00
Sends the Probe X past the
reference flag.
XREF Probe X setting ref The XREF position reported here is XREF
Active = Y [0] cmd, lastcmd G 897.
xref 0/897 last 0 / 0
Sends the Probe X past the
Probe X reference test reference flag 3 times.
xref 0/897 last 0/897 Probe X position in inches
xref 0/10237 last 0/10235 motor Probe X position in motor counts
XREFTST steps xref 0/897 last 0/896
xref 0/10237 last 0/10216 motor XREFTST
steps xref 0/897 last 0/896
xref 0/10237 last 0/10216 motor
steps
Displays status of the Probe X
Evaluate X reference values reference.
Probe X pos=51, lastdest=50,
XLIST XLIST
state=G[0]8901
Active =Y[0],
xref 0/897 last 0/896
Exercises Probe X movement full
Show X cycle time stroke 20 times.
XTIM X time = 19.1 sec Displays a value in seconds. XTIM

Sends the Probe X to various Checks the movement of the Probe


PRXTST locations X and displays a time in seconds PRXTST

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3.7 Probe Z
Probe Z – this section contains Service Instructions, Troubleshooting Guide, Error Messages
and Command List

The Probe Z assembly has been designed to move the probe tip up and down, when aspirating
and dispensing liquids. The Probe Z also has an oscillating mixing action to stir the reaction
after reagent and sample have been dispensed. The Probe Z has a number of replaceable
parts, please see the diagram below. Service Instructions for replacing the part(s) are given
below or they are available for qualified service technicians to view in video form over the
internet. The Liquid Sensing feature automatically detects when the probe tip comes in contact
with a liquid substance. The ChemWell®-T also features Probe Z jam detection. This feature
will stop the Probe Z in the downward direction if a cap is left on the bottle. There are
numerous critical adjustments that can affect the results of your chemistries, only qualified and
trained technicians should attempt to make these adjustments.

Service: The Probe Z is designed to give years of trouble free service. There are a few
items that may need attention or replacement during the life of the instrument. They are:

1. Motor, Mixer (part #105061SP-46)

2. Motor, Probe Z (part #994369SP)

3. Belt, Mixer (part #132081SP)

4. Belt, Probe Z (part #132080SP)

5. Probe Z Fluid Sense Wire (part #993293SP)

6. Probe Tip Assembly (Part # 994617SP)

7. Counterweight Assembly (Part # 994622SP)

8. Mixer Assembly (Part # 994620SP)

9. Spindle Bearing Assembly (Part # 994621SP)

10. Motor Controller Board Z Mover (Part # 994636SP)

11. Optical Fluid Sensor (Part # 995576SP)

Covers:

Each item is given step by step instructions below. For service to the Probe tip or mixer,
remove the Mixer Cover. For Service to the board, Probe Z belt, or Probe Z Motor, remove the
Probe Z Cover. In some cases, it may be better to have both covers removed. To perform
maintenance or repair, remove either/or both covers as instructed below. The Mixer Cover is
held on with 2 screws, the Probe Z Cover is held on with 3 screws. These 5 screws are
intentionally short and must not be replaced with longer screws.

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Probe Z (Continued)

Remove the two Mixer Cover screws and the three Probe Z Cover screws first Mixer Cover

Note: Do not replace these screws with longer screws!

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Motor Controller Z Mover

Optical Fluid Sense PCB

Mixer Motor Belt, Probe Z Probe Z Motor

Belt, Mixer Counterweight Probe Tip Spindle Bearing Assembly

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3.7.1 Motor, Mixer (part #105061SP-46)
1. To replace the Mixer Motor, the screws will need to be removed from the bottom of the
motor. These screws can be access through holes provided in the bottom of the mixer
bracket.

Z Wire Bracket Mixer Motor bracket

Mixer Motor Motor mounting screws Access holes through the mixer bracket

2. Since the Mixer Motor is shipped with a new wire and connector, it will be necessary to
carefully free the old wire by cutting the wire ties and unplugging the connectors from
the Z Motor Controller board. Note: The routing of the wire and tubing is critical to
the correct operation of the instrument. Pay close attention to this routing and
make sure it is duplicated during re-assembly.

3. Loosen the Z wire bracket to allow the Mixer motor to lift off the mixer motor bracket.
The Z wire bracket can be twisted or bent to achieve the desired routing for the wire
and tubing during reassembly. The wire bundle must clear and not rub on the Probe Z
cover.

4. Re-install the new motor in the reverse of above. The mixer belt will need to be re-
installed on the pulley.

5. Check the mixer belt for clearance between the bottom of the Sense PCB and the
Spindle housing. Adjust the mixer motor pulley as needed.

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Motor, Mixer (Continued)

Check mixer belt clearance between bottom of the Sense PCB and the Spindle
housing. Adjust the mixer motor pulley up or down as needed.

Note: Mix speeds are programmable and changeable in the PLIST. Mix speeds can affect the
performance and data of the assay. Whenever changing the mixer belt or motor, verify mixer
speeds.

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3.7.2 Motor, Probe Z (part #994369SP)
1. To replace the Probe Z motor, start by removing the belt from the motor pulley.

2. Disconnect the motor wire connector from the top of the Z Motor Controller board.

3. Unplug Ground cable from ground terminal on top of probe Z.

Belt Probe Z Motor Ground Wire Motor Connector

Mounting screws

4. Remove the 2 motor mounting screws that hold the motor. Note there are different
length screws.

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Motor, Probe Z (Continued)

5. Maneuver motor downward and out. If pulley does not fit through the hole, loosen top
right screw on the Z board and move the bracket out of the way.

6. Pull pulley off the old motor shaft and push onto the new motor shaft. Install new motor
in the reverse order of removal. Be careful to keep the wiring away from the encoder
wheel and in a way to allow the Probe Z cover to be re-installed correctly.

New Probe Z motor installed

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3.7.3 Belt, Mixer (part #132081SP)
1. To install a new Mixer belt it will be necessary to remove the Probe tip and
counterweight. See the section on Probe tip and counterweight.

2. Unplug the top connector and remove the 2 screws in the Sense PCB. Slide the board
to the left and away from the spindle and mixer assembly. Be careful not to lose the 2
nylon spacers between the board and the bracket.

Remove the 2 screws in the Sense PCB Slide the PCB board to the left

2. With the Probe tip, counterweight, and Sense PCB removed, the new belt can be
slipped over the Spindle and pushed back under the Mixer motor bracket.

3. Use a small tool that will not damage the o-ring belt to maneuver the belt onto the Mixer
motor pulley. This can be done by working from the left side of the Probe Z and
carefully moving the belt into position.

Slip the new belt over the Spindle and push it back under the Mixer Motor bracket

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Belt, Mixer (Continued)

4. Re-assemble the Sense board and Probe tip in the reverse order. Check the belt
clearance between the bottom of the Sense board and the back edge of the Spindle
assembly. Adjust the Mixer motor pulley if needed.

Note: Mix speeds are programmable and changeable in the PLIST. Mix speeds can
affect the performance and data of the assay. Whenever changing the mixer belt or
motor, verify mixer speeds.

3.7.4 Belt, Probe Z (part #132080SP)


1. To replace the Probe Z belt, the encoder wheel will need to be removed.

2. Unplug connector from encoder board.

3. Remove the 2 screws in the top of the encoder wheel then carefully lift the wheel off the
pulley and away from the encoder. Be careful not to damage the encoder.

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Belt, Probe Z (Continued)

4. Replace Probe Z belt

5. Reinstall encoder wheel and screws (be careful not to damage optic sensors).

Use caution not to damage the encoder when removing or re-installing encoder wheel.

6. Plug in encoder connector.

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3.7.5 Probe Z Fluid Sense Wire (part #993293SP)
1. The Probe Z Fluid Sense wire can be replaced by unplugging the connector from the
Sense board and loosening the screw in the counterweight.

2. With the counterweight screw loose, pull the sense wire out of the counterweight.

3. To install a new wire is the reverse of the above. Be careful not to allow the wire to
stick out the bottom of the counterweight.

Fluid Sense Wire Loosen screw in counterweight and pull sense wire out of counterweight

3.7.6 Probe Tip Assembly (Part # 994617SP)


1. The Probe tip assembly is a critical part of the ChemWell®-T. To replace the probe tip it
will be necessary to remove the tubing from the top of the Probe tip. A small
piece of fine sandpaper can help with gripping the tubing.

Remove tubing from the top of the Probe tip

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Probe Tip Assembly (Continued)

2. Loosen the screw in the counterweight and slide the Probe up and out of the
counterweight and spindle.

Loosen screw in the counterweight (Phillips #1 screwdriver) then slide the Probe up and out of
the counterweight and spindle

Lift the Probe tip up and out of the counterweight and spindle

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Probe Tip Assembly (Continued)

3. Slide the new Probe tip back into the counterweight and spindle in the reverse of
above. The Probe tip has a stop collar that is designed to stop against the top of the
counterweight. Gently re-tighten the screw in the counterweight to hold the Probe tip in
position. NOTE: Make sure fluid sense wire is properly connected.

Stop collar Probe Tip Assembly

Probe tip installed in counterweight and spindle

4. Re-connect the tubing and push it all the way down on the Probe Tip. A small piece of
fine sandpaper can help to grip the tubing and push it down. The tubing should be
down to the top of the stop collar.

Before running assays, be sure to prime the system using the prime syringe tool in the
software. Then, check the probe alignment to the Reagent Rack, wash cup, and vessel
rack referring to the Alignment Set-Up Section (4.2) in the Operator's Manual.

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3.7.7 Counterweight Assembly (Part # 994622SP)
1. To remove the counterweight, remove the tubing from the top of the Probe tip. See
section 6 for this procedure.

2. Unplug the Sense wire from the Sense PCB.

3. Loosen the screw in the front of the counterweight that holds the Probe tip then slide
the Probe tip up and out of the counterweight and spindle. See section 6 for this
procedure.

4. The counterweight can now be removed and serviced as needed.

Counterweight Assembly Black plastic faces to the right

Hole for Sense Wire Hole for Probe Tip

3.7.8 Mixer Assembly (Part # 994620SP)


Mixer Mixer
Assembly Assembly
shown shown
without with the
the wiring wiring
attached attached
and the and the
Probe Tip Probe Tip
removed installed

1. To change the Mixer Assembly, the Probe tip and counterweight should be removed
first. This will eliminate any chance of damage to the Probe tip during this procedure.

2. Unplug the mixer motor connector and the Sense board connector from the Z Mover
Motor Controller board.

3. Carefully cut the wire ties that fasten the wire bundle from the Z Mover Motor
Controller board to the Z wire bracket on the Mixer Motor bracket. Pay special
attention to the way the wires are routed as this routing is critical to the correct
operation of the Probe Z.

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Mixer Assembly (Continued)

4. Use a small Phillips screwdriver and remove the 4 pan head screws that hold the Mixer
Assembly to the Probe Z truck. Pay attention to the position of the metal flag under the
2 top screws as this will need to be put back into the same position during re-assembly.

Metal Flag

Use a Phillips screwdriver and remove the 4 pan head screws that hold the Mixer Assembly

5. Re-assembly the Mixer Assembly in the reverse order of above. Make sure the 4 pan
head screws that hold the assembly are tight. Note: Loose screws can affect the
truck adjustment.

6. Check to make sure the Mixer Bracket is horizontal and at 90 degrees to the front of the
Probe X rail. This should be checked with a square. If adjustment is needed, loosen
the 4 pan head screws and rotate the bracket until it is at 90 degrees to the Probe X
rail.

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Mixer Assembly (Continued)

Mixer Bracket Front of Probe X Rail 90 degree angle

7. Re-attach the wiring connectors on the Z Mover Motor Controller board and use wire
ties to bundle the wiring and tubing in the same manner as it was before disassembly.
This must be done correctly for correct operation of the instrument and to
prevent damage to the tubing or wiring.

3.7.9 Spindle Bearing Assembly (Part # 994621SP)


1. To remove the Spindle Bearing Assembly, start by removing the Probe Tip and
counterweight assembly. (See sections 6 and 7)

2. Remove the Sense board by removing the 2 screws in the front of the board,
disconnecting the top connector and sliding the board out to the left.

3. Remove the Mixer belt by slipping it over the spindle.

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Spindle Bearing Assembly (Continued)

4. Remove the 2 small screws that hold the Spindle Bearing assembly and push the
assembly up and out of the mounting hole in the Mixer bracket.

Remove the 2 small screws that hold the Spindle Bearing assembly Spindle Bearing
Assembly

3.7.10 Motor Controller Z Mover (Part # 994636SP)


1. To replace the Z Mover Motor Controller board, start by disconnecting all the
connectors that are plugged into the board. Make sure to note the location of all
connectors.

2. The new board is partially assembled with the PCB bracket mounted to the upper left
side of the board. (looking at it from the rear of Probe Z). Remove the ground wire and
screw that holds the ground strap and PCB bracket to the motor mount.

Remove the screw and ground terminal that holds the PCB bracket to the motor mount

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Motor Controller Z Mover (Continued)

3. Remove the 2 bottom screws from the Z Motor Controller Board and the board should
now be free from the Probe Z.

Remove the 2 bottom screws from the Z Motor Controller board

4. Remove the wire ties from the bottom holes of the new board. These are only to hold
the nylon spacers from getting lost.

5. Re-mount the Z Motor Controller board in the reverse order of above. Make sure the
nylon spacers are in between the boards when re-installing the bottom screws. There
are also nylon washers between the board and the mounting bracket on the bottom
holes.

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Motor Controller Z Mover (Continued)

Spacers between the board and the mounting bracket on the bottom holes

6. Plug in all connectors to the board as they were before unplugging them. The
communication cable faces with the red stripe toward the center of the board.

Reconnect the ground wire.

3.7.11 Optical Fluid Sensor (Part # 995576SP)

1. Unplug green connector.

2. Carefully remove tubing from Probe tip by sliding it up and off the Probe.

3. Unplug sense wire and remove probe tip assembly and counterweight.

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Optical Fluid Sensor (Continued)

4. Remove sensing board by unscrewing the 2 screws then remove board to the left. Be
careful not to damage the optical sensors that are above and below the spindle

Remove Sense board by removing the 2 screws, then remove the board to the left. Be
careful not to damage the optical sensors that are above and below the spindle

5. Reinstall sensing board in the reverse order of above being careful not to bend the
optical sensors when doing this. Make sure the 2 nylon washers are on the back side
of the board. These keep the board from shorting to the metal bracket.

6. Install probe tip assembly and counterweight. Plug sense wire back into sense board
and re-connect the green connector.

2 nylon washers go between sense board and mounting bracket

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3.7.12 Troubleshooting Guide for Probe Z

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION

Probe Z does Power not turned on Check for power on the instrument
not move
Check heart beat light on main board.

USB cable not connected Check status in command window. Replace cable.

Mechanical Jam Check that Probe Z moves freely - turn lead screw.

Lubricate rails and lead screw with oil.

Bearing defective.

Broken belt Replace black belt.

Electrical problem Check for broken wires.

Check all connectors are properly plugged into Z


motor board.

Probe Z noise Loose fastener Check for loose screws on Probe Z

Truck out of adjustment Adjust truck tension.

Damaged lead screw Replace lead screw.

Motor settings Verify motor settings.

Motor Noise Replace motor

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Troubleshooting Guide for Probe Z (Continued)

POSSIBLE
PROBLEM CAUSE POSSIBLE SOLUTION

Probe Z not running Rails or lead screw


smooth dry Clean and lubricate rails and lead screw with oil.

Board problem Replace Z mover board

Wire/tubing rubs on Inspect tubing and wires, reroute as needed.


cover

Belt worn Replace belt

Probe sense issue Defective sense


board Replace sense board

Loose sense wire Tighten counterweight screws to sense wire.

Broken wire Repair or replace wire

Ground problem. Check all grounds for resistance.

Mixer not working Belt broken Replace mixer belt.

Counterweight Pull counterweight down by pulling on probe tip.


jammed

Mix speed setting Check mix speed

Motor defective Change mixer motor.

Broken wire Repair or replace wire.

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3.7.13 Probe Z Error Messages

*002: {Probe Z} command exceeds allowed range at {CurPos} of >{ReqPos}<

Meaning of Error: position sent to mover exceeds maximum range specified at the
mover module

Possible Cause: Encoder problem; corrupted command;

Possible Solution: Replace encoder; restart instrument and software

*002: {Probe Z stat timeout at ?? of >{ReqPos}<

Meaning of Error: mover module did not respond after a few tries

Possible Cause: Electrical problem; communication issue

Possible Solution: Check all connectors and ribbon cable; restart instrument and
software

*002: {Probe Z} break cmd recvd at {CurPos} of >{ReqPos}<

Meaning of Error: mover module responded with an interrupt indication

Possible Cause: The Probe Z was interrupted during the expected move

Possible Solution: Check for mechanical sources that may have caused an interrupt
during the move. Clear area of source of interrupt.

*005: Probe Z Fluid not detected in Range

Meaning of Error: fluid sense did not occur before search range exceeded, check max Z

Possible Cause: Fluid sense issue; no fluid in bottle; defective sense board, bad
connection on sense wire or Probe.

Possible Solution: No fluid in bottle; replace Sense board; tighten screws in


counterweight on sense wire and probe tip.

* 006: Probe Z is timing out (10 tries)

Meaning of Error: Probe Z module did not respond

Possible Cause: Defective Z Motor Controller board; defective encoder

Possible Solution: Replace Z Motor controller board, check all connections and ribbon
cable; replace encoder

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Probe Z Error Messages (Continued)

*006: Probe Z is jammed at {CurPos} (pcmd)PRZ

Meaning of Error: mover is reporting a jam

Possible Cause: Connector not connected properly; encoder damage; mechanical


jam; motor issue; broken wire

Possible Solution: Connect all connectors properly; replace encoder; repair


mechanical issue; replace motor; repair broken wires

*006: Probe Z has tip jam at {CurPos} of {ReqPos}

Meaning of Error: Probe Z tip is reporting a jam (indicated by jam light illuminated)

Possible Cause: Probe tip is hitting bottle cap; probe tip is stuck in up position

Possible Solution: Check alignment of Probe with alignment procedures; free probe tip
from stuck position by pulling it down; replace sense board or optical sensors.

*006: Probe Z has mech jam at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: mover module is reporting a stuck condition

Possible Cause: Mechanical jam; encoder damage; defective motor; broken wire;
connector not connected properly

Possible Solution: Repair mechanical issue; replace encoder; replace motor; repair
broken wire; connect all connectors properly

*006: Probe Z has mech jam (long running) at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: mover module has been running and still not reached destination

Possible Cause: Mechanical issue; instrument detected movement but insufficient to


make it to the required destination

Possible Solution: Repair mechanical issue

*006: Probe Z has mech jam (EndLimit) at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: mover module report mechanism has reached the limit sensor

Possible Cause: Defective encoder; home flag position not set correctly; Probe Z
traveled past home sensor and became stuck at the top

Possible Solution: Replace encoder; reset home flag position to allow more travel past
home; restart instrument and software

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Probe Z Error Messages (Continued)

*015: Probe Z move timeout at ?? of >ReqPos}<

Meaning of Error: mover issued bad status

Possible Cause: Bad or corrupted command issued.

Possible Solution: Check command syntax; restart instrument and software

3.7.14 Probe Z Command List

Name Description Explanation Syntax

Probe Z home Moves the Probe Z to the home


PRZH position PRZH

Probe Z to position Moves the Probe Z to a position. PRZM


This value is displayed in inches. nnnn
PRZM
Example- to move the Probe Z to PRZM
2.00" 200

Probe Z current status Displays the current status of the


Probe Z.

ZSTAT ProbeZ pos = 198 ProbeZ position in inches 1.98 ZSTAT


Lastdest = 200 Last destination requested in inches
state = G [0] 0 2.00 state encoder counts = 3058
Current count = 3058

Show Z cycle time Exercises Probe Z movement full


ZTIM stroke 20 times. ZTIM
Z time = 19.1 sec Displays a value in seconds.

Probe Z movement test Moves the Probe Z to various test


positions.
PRZTST PRZTST
prZ time = 19.1 sec Used to check fluid movement of
Probe Z to various positions with a
time value displayed.

ZINIT Probe Z mover initialize Sends settings from flash to main ZINIT

MIX Mix at current position Sends the Probe Z down to mix at MIX
the current Probe X and Cuvette
Mover positions.

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Probe Z Command List (Continued)

Name Description Explanation Syntax

MXOFF Turns off probe mix If the mixer is running, it will turn off. MXOFF

MXON Turns on probe mix The mixer will turn on. MXON

MXSPD Sets probe mix speed Sets the default mix speed. MXSPD
nn

70 = MixSpeed_Default Example speed is set to 70 MXSPD


70

PARK Park probe at wash cup Sends the Probe X to the wash cup PARK
and parks there.

PCNT Probe current sense Example Current count = 3061 PCNT


reading

PSEN Probe Sense Threshold Sets or displays probe sense PSEN nn


setting threshold

90=ProbeSense_Threshold Example PSEN 90


was 100 Current count =
3060

PFFR Probe Z fluid detection Sends Probe Z down until it detects PFFR
fluid.

Probe Z sense detected Example – Probe Z went down and


Fluid sensed at 153, count detected fluid at 1.53”, it stopped at
= 2403 (S) EP : Final 1.52”
count = 1942 Probe Z
pos=152, Lastdest=326,
state=S Probe X pos=300,
Lastdest=300, state=G

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3.8 Rack Mover
RACK MOVER – this section contains Service Instructions, Troubleshooting Guide, Error
Messages and Command List

The Rack Mover transfers the reagents and samples from front to back, when placed on the
Universal Rack. The Rack Mover has a Peltier cooling system that cools the three left hand rows
of reagents. The Peltier is fan cooled and is capable of maintaining the liquid temperature in the
bottle at row A approximately 10˚C from the ambient room temperature. The movement of the
Rack is via a DC motor with a 12 position encoder wheel. Power is transferred from the DC motor
into the lead screw. There is a home position switch mounted at the front. There is also an over
travel protection switch mounted at the rear.

Service: The Rack Mover is designed to give years of trouble free service. There are a few items
that may need attention or replacement during the life of the instrument. They are:

1. Motor (part #994640SP)


2. O-ring Rack (part #132060SP)
3. Encoder Board (part #995573SP)
4. X Mover Motor Controller Board (part #995565SP)
5. Opto Right Angle (part #995571SP)
6. Cooling Assembly (part #994344SP) with Graphite Sheet (part
#13503240SP)
7. Fan, Rack Mover Cooling (part #104507SP)

Each item is given step by step instructions below. To service the following items, it will be
necessary to remove the Rack Mover from the instrument. Please see Section 4 on removing the
Rack Mover. NOTE: When service is performed on Items 1 through 5, the cooling assembly can be
left in the chassis.

Cooling assembly Motor Belt

Opto Right Angle Fan Encoder

FINGER ACCESS HOLE TO CLEAR MECHANICAL JAM

NOTE: Rack Mover shown as transparent for clarity of parts identification

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3.8.1 Motor (part #994640SP)
1. To replace the Motor, start by turning the Rack Mover upside down and locating the
nylon pulley and o-ring belt. Slide the o-ring belt off the nylon pulley then pull the pulley
off the motor shaft.
2. With the nylon pulley removed, the 2 pan head screws holding the motor to the motor
bracket can be removed.

Slide o-ring belt off nylon pulley Pull nylon pulley off motor shaft

Remove 2 pan head screws holding motor to motor bracket

3. Install the new motor in the reverse order being careful to align the nylon pulley with the
encoder wheel pulley. Re-route the wiring in the same manner as it was with the old
motor.
4. Re-install the o-ring belt and check for correct alignment of the belt.

3.8.2 O-ring Rack (part #132060SP)


1. To replace the o-ring, it will be necessary to separate the cooling assembly from the
Rack mover if it has not already been separated. To separate the cooling assembly
from the rack mover, remove the 4 flat head screws in the top surface of the blue
transfer plate. Care should be taken not to damage the Graphite Sheet (part
#13503240SP) during the assembly process. If the Graphite Sheet gets torn or
damaged; it will need to be replaced.

Remove the 4 flat head screws in the top surface of the blue transfer plate

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O-ring Rack (Continued)

Cooling assembly Graphite sheet goes on this surface

2. Remove the 2 pan head screws that hold the encoder bracket to the motor bracket and
mover the encoder away from the encoder wheel. Be careful not to damage the
encoder.

Remove the 2 pan head screws that hold the encoder bracket to the motor bracket

3. The new o-ring can now be installed over the encoder wheel pulley and the motor drive
pulley.

New o-ring can now be installed over the encoder pulley and the motor drive pulley

4. Re-install the encoder mounting bracket using the same screws that were taken out.
Be careful not to damage the encoder.

5. Re-install the cooling assembly by positioning the graphite sheet between the cold
block and the blue transfer plate and then re-install the flat head screws in the top
surface of the transfer plate.

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3.8.3 Encoder Board (part #995573SP)
1. Remove the 2 pan head screws that hold the encoder board to the bracket. Be careful
not to lose the 2 nylon spacers on the back side of the board.

Encoder board shown with 2 mounting screws removed

2. Position the new encoder board in the same manner as the old board and re-install the
2 nylon spacers between the board and the encoder bracket, then re-install the screws
that were removed.
3. Re-install the encoder bracket to the motor support bracket using the same pan head
screws and washers that were removed.

Re-install the encoder bracket to the motor support bracket using the pan head screws and washers

4. Check that the encoder wheel is not able to touch the encoder during operation. If the
encoder wheel needs to be adjusted, loosen the set screw in the encoder wheel pulley
and adjust as needed.

Check encoder clearance to encoder wheel.

Adjust encoder wheel pulley as needed.


5. Re-attach the wiring to the encoder.

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3.8.4 X Mover Motor Controller Board (part #994633SP)
1. The X Motor Controller Board is mounted on 2 sheet metal brackets. The board
controls the motors on the Rack Mover and the Probe X. To remove the board, remove
the 2 pan head screws that hold the mounting brackets to the chassis.

2. Lift the board up and disconnect the connectors. Remove the disconnected board
from the instrument.

3. Remove the mounting brackets and nylon spacers from the board and re-assemble the
new board with the mounting brackets and nylon spacers in the same manner.

X Mover Motor Controller Board

4. Re-connect the wiring connectors to the board in the reverse order that they were
disconnected.

5. Re-install the pan head screws in the mounting brackets and re-fasten to the chassis.

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3.8.5 Opto Right Angle (part #995571SP)
1. Remove the Cooling assembly (see above). There are 2 Opto Right Angle parts on the
Rack Mover. These are used as the home sensor and the other is used as a forward
position over travel sensor. Care must be taken not to damage or bend the opto
sensors while working on the Rack Mover. If an optical sensor gets bent for some
reason, most often, it can be restored to its original position by bending it back into
place. Remove the 2 pan head screws at each end of the actuator assembly and lift
the actuator assembly off the Rack Mover.

Pan Head screws Opto right angle home Opto right angle over travel

2. With the actuator assembly removed, the attachment screws for each opto is
accessible with a screwdriver under the lead screw. Be careful not to lose the
washers and nylon spacers that are part of the opto assembly.

Actuator assembly removed Access to home position opto screw

Actuator assembly removed Access to over travel opto screw

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Opto Right Angle (Continued)

3. Once the opto has been re-installed, place the actuator assembly back into the Rack
Mover and re-install the 2 pan head screws and washers that were removed. Be sure
to line up the pin on the contactor with the slot in the lead nut. If the contactor was
removed during this process, make sure the single pin faces the rear of the instrument
before securing the actuator assembly.

Single pin in Contactor faces towards the rear of the instrument

3.8.6 Cooling Assembly (part #994344SP) and Graphite Sheet (part


#135032SP)

1. Remove the Cooling assembly (see above). Care should be taken not to damage the
Graphite Sheet (part #13503240SP) during the assembly process. If the Graphite
Sheet gets torn or damaged; it will need to be replaced.

2. Replace the Cooling assembly with the new unit.

Cooling assembly Graphite sheet goes on this surface of cold block

3. Insert the graphite sheet between the cold block and the blue transfer plate so that
the graphite sheet covers the entire cold block top surface. Once positioned
correctly, screw the parts together. (See above section)

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3.8.7 Fan (part #104507SP)

1. Remove the 2 pan head screws that hold the fan bracket to the heat sink.

Remove the 2 pan head screws that hold the fan bracket to the heat sink

2. Remove the 2 screws that hold the fan to the fan bracket. Be mindful of the wire
routing so the new fan wire will route the same way.

Air Flow
Remove the 2 screw that hold the fan to the fan bracket

3. Install the new fan in the reverse order. Make sure the arrow on the fan housing is
facing towards the heat sink so that the air flow will force the warm air down and out of
the instrument. The fan will not change direction by changing the wiring polarity.

4. Re-install the fan bracket with fan attached to the side of the heat sink in the reverse
that it was removed.

5. Re-install the cooling assembly to the Rack Mover by placing the graphite sheet
between the cold block and the blue transfer plate, then install the 4 flat head screws
thru the transfer plate and into the cold block.Care should be taken not to damage the
Graphite Sheet (part #13503240SP) during the assembly process. If the Graphite
Sheet gets torn or damaged; it will need to be replaced.

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3.8.8 Troubleshooting Guide for Rack Mover

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Rack Mover Power not turned on Check for power on the instrument
does not
move Check heart beat light on main board.

USB cable not connected Check status in command window. Replace cable.

Mechanical Jam Check that Rack moves freely - turn lead screw.
Lubricate rails and lead screw with oil.
Rack jammed against front Cover, turn encoder
wheel with finger in the access hole.

Broken belt Replace O-ring belt.

Electrical problem Check for broken wires.


Check all connectors are properly plugged into
motor board.
Rack Mover Loose fastener
Check for loose screws.
noise

Motor Bracket loose Tighten motor mounting bracket.

Damaged lead screw Replace lead screw.

Motor settings Verify motor settings.

Motor Noise Replace motor


Rack Mover Lead screw dry
not running Clean with isopropyl alcohol and lubricate lead
smooth screw with oil.

Board problem Replace mover board

Sliding surface for Rack


is worn. Replace protective tape on deck.

Belt worn Replace belt

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3.8.9 Rack Mover Command List

Name Description Explanation Syntax


RHOME Rack to home Moves the Rack to the home position RHOME
Moves the Rack to a position. This value RMOVE nnnn
Rack move to is displayed in inches.
RMOVE
position
Example- to move the Rack 4.00" RMOVE 400
Rack current status Displays the current status of the Rack
RRack pos = 399 Rack position in inches 3.99
RSTAT RSTAT
Lastdest = 400 Last destination requested in inches 4.00
state = G [0] 0 state
Show Rack cycle Exercises Rack mover full stroke times.
RTIM time RTIM
R time = 16.1 sec Displays a value in seconds.

Rack mover
RINIT RINIT
initialize Sends settings from flash to main
COOLON Reagent cooling on Turns on the reagent cooling. COOLON
COOLOFF Reagent cooling off Turns off the reagent cooling. COOLOFF
Reagent cooling Displays current reagent cooling
temperature temperature.
COOLTEMP Reagent Cooling is Cooling status (on or off) COOLTEMP
off Cool temp 0.0, Cooling temp from thermistor
Hot temp 46.5
Set cooling group Sets cooling group from the Plist PTSETCG n n n
Reagent min temp Example - sets the minimum temp to -10
setting ˚C and sets the maximum hot side to 70
PSETCG Reagent HotMax ˚C and the control default as 1
PTSETCG -10 70 1
Setting
Reagent Control
Default

3.8.10 Rack Mover Error Messages

*002: Rrack command exceeds allowed range at {CurPos} of >{ReqPos}<

Meaning of Error: position sent to mover exceeds maximum range specified at the
mover module

*002: Rrack stat timeout at ?? of >{ReqPos}<

Meaning of Error: mover module did not respond after a few tries

*002: Rrack break cmd recvd at {CurPos} of >{ReqPos}<

Meaning of Error: mover module responded with a interrupt indication

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Rack Mover Error Messages (Continued)

*008: Rrack is timing out (10 tries)

Meaning of Error: PRZ module did not respond

*008: Rrack has mech jam at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: mover module is reporting a stuck condition

*008: Rrack has mech jam (long running) at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: mover module has been running and still not reached destination

*008: Rrack has mech jam (EndLimit) at {CurPos} of {LastDest} req->{ReqPos}

Meaning or Error: mover module report mechanism has reached maximum travel

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3.9 Shuttle

SHUTTLE – this section contains Service Instructions, Troubleshooting Guide, Error Messages
and Command List
The Shuttle moves the cuvette from the Cuvette Carrier into the Photometer to take readings. The
mechanism is similar to the Probe Z. It has guide rods, a truck, lead screw and motor. There is a
home switch mounted on the RH Shuttle Bracket. The comb switch ensures that the Cuvette is
centered in the well and the correct position is being read. The comb switch is set at the factory
and should not be adjusted. The truck has a set screw adjustment for tension on the guide rods.
The cam is adjustable for when the gate opens and closes to pull the Cuvette in and out of the
Photometer.

The Shuttle is designed to give years of trouble free service. There are a few items that may need
attention or replacement during the life of the instrument. They are:

1. Motor (part #994641SP)


2. Oring shuttle (part #132062SP)
3. Encoder Board (part #995573SP)
4. Opto Straight (part #995568SP)
5. V Mover Motor Controller Board (part #994632SP)

Each item is given step by step instructions below.

O-Ring Motor Encoder Board Opto Straight

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Service of Shuttle:
It is recommended that the Shuttle be removed from the instrument to perform service on the
following items. Please refer to Section 4 for Shuttle Removal.

V Mover Controller Board

3.9.1 Motor (part #994641SP)


1. The motor is attached to the right hand bracket by 2 screws. To access the 2 motor
screws, the nylon pulley must be removed from the motor shaft. Slide the O-Ring off
the motor pulley then pull the pulley off the shaft. The pulley is a press fit on the shaft.
It may be helpful to use a screwdriver behind the pulley to help move it off the shaft.
Be careful not to damage the nylon pulley.

2. Once the pulley is removed the 2 motor mounting screws are accessible. Remove the
screws and the motor will be released from the bracket.

3. Re-attach the motor to the bracket using the same screws that were removed.
4. Press the nylon pulley back on the motor shaft and line up groove with the encoder
wheel pulley.
5. Re-install the O-Ring back into the pulley groove.

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3.9.2 O-ring Shuttle (part #132062SP)
1. To replace the O-Ring it will be necessary to create a gap between the encoder wheel
and the encoder. This can be done by removing the screws from the encoder
mounting bracket and sliding the encoder out of the way.
2. Once the encoder is away from the encoder wheel, the new O-Ring can be installed
around the nylon pulley on the motor and the encoder wheel pulley. Due to the
closeness of the encoder wheel to the bracket, it may be necessary to put the O-Ring
in a slot on the encoder wheel and rotate it past the edge of the bracket.
3. With the O-Ring installed on the pulleys, re-install the screws in the encoder mounting
bracket. Care must be taken not to damage the encoder. The encoder wheel should
rotate freely within the encoder and should not be able to touch the encoder in any
condition.
4. Adjust the encoder wheel pulley as needed by loosening the set screw and moving it
on the shaft to achieve a free running condition.

3.9.3 Encoder Board (part #995573SP)


1. To replace the Encoder board, first disconnect the 4 position connector from the
header.

2. Remove the screws from the encoder mounting bracket.

3. Remove the screw from the encoder board. Be careful not to lose the nylon spacers
under the board.

4. Re-install the nylon spacers under the new encoder board then install the same screws
that were removed from the old board.

5. Re-install the encoder mounting bracket being careful not to damage the encoder.

6. Re-attach the 4 position connector to the encoder board.

7. Adjust the encoder wheel pulley as needed by loosening the set screw and moving it
on the shaft to achieve a free running condition.

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3.9.4 Opto Straight (part #995568SP)
1. There are 2 optical sensors (Opto Straight) used on the Shuttle. One is for the home
position and the other is for the comb switch. The sensor for the comb sensor bracket
has been pre-set at the factory and if this sensor is replaced, the procedure for
resetting the comb sensor will need to be done. This opto can be replaced without
moving the mounting bracket.

Home Opto Comb Switch Opto

2. To replace the home opto, remove the 4 position connector from the board and remove
the mounting bracket screw, washers and spacer. Change the opto and re-attach to
the Shuttle right hand bracket with the spacer, washers and screw that was removed.

3. Re-attach the 4 position connector and check for clearance on the home end of the
comb bracket.

3.9.5 V Mover Motor Controller Board (part #994632SP)


(It is not necessary to remove the Shuttle in order to replace the V Mover Motor Controller
Board)
1. The V Mover Motor Controller Board is located in the right, rear of the instrument. To
access this board set it is necessary to remove the Enclosure cover (see Section 3). To
remove the board, remove the 2 screws that hold the board bracket to the chassis
base. It is not necessary to remove the support post in front of the board but it may be
unscrewed and removed for easier access to the board.

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V Mover Motor Controller Board

2. Lift the board and carefully disconnect the ribbon cables and connectors that attach
to it. Please note that this board controls both the Shuttle and the Cuvette Mover.
The board has headers on each side, one side is for the Shuttle and the other side
is for the Cuvette Mover. The motor headers are located on the top of the board.
3. Re-connect the wire connectors in the reverse order being careful to correctly
position all connectors on the headers. (It is easy to miss a pin when plugging in
the connectors)
4. Re-attach the board bracket to the chassis base with the same screws that were
taken out.
5. Re-install the support post if it was removed.

3.9.6 Troubleshooting Guide for Shuttle

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Shuttle does Power not turned on Check for power on the instrument
not move
Check heart beat light on main board.
USB cable not Check status in command window. Replace
connected cable.
Mechanical Jam Check that Shuttle moves freely - turn lead
screw.
Lubricate rails and lead screw with oil.
Check that lever arm is not jammed on cam and
that gate operates freely.

Broken belt Replace O-ring belt.

Electrical problem Check for broken wires.


Check all connectors are properly plugged into
motor board.

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Troubleshooting Guide for Shuttle (Continued)

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Shuttle noise Loose fastener Check for loose screws.

Motor Bracket loose Tighten motor mounting bracket.

Damaged lead screw Replace lead screw.

Motor settings Verify motor settings.

Motor Noise Replace motor

Shuttle not
Clean with isopropyl alcohol and lubricate lead
running smooth
Lead screw dry screw with oil.

Board problem Replace mover board

Belt worn Replace belt

3.9.7 Error Messages for Shuttle

*002: {V Shuttle} command exceeds allowed range at {CurPos} of >{ReqPos}<

Meaning of Error: Position sent to mover exceeds maximum range specified at the
mover module

*002: { V Shuttle} stat timeout at ?? of >{ReqPos}<

Meaning of Error: Mover module did not respond after a few tries

*002: { V Shuttle} break cmd recvd at {CurPos} of >{ReqPos}<

Meaning of Error: Mover module responded with a interrupt indication

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Error Messages for Shuttle (Continued)

*100: V Shuttle has mech jam at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: Mover module is reporting a stuck condition

Possible Cause: Wire disconnected; encoder not working; gate jammed or cuvette
jammed

*100: V Shuttle has mech jam (long running) at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: Mover module has been running and still not reached destination

Possible Cause: Shuttle is trying to move by has too much resistance to move; motor
brushes worn out; truck too tight; lead screw dirty

Possible Solution: replace motor; clean and lubricate lead screw; rails; re-adjust truck

*100: V Shuttle has mech jam (EndLimit) at {CurPos} of {LastDest} req->{ReqPos}

Meaning of Error: Mover module report mechanism has reached the limit sensor

*015: V Shuttle move timeout at ?? of >ReqPos}<

Meaning of Error: Mover issued bad status

*102: No Read

Meaning of Error: Mechanical error Read command was cancelled due to mechanism
issue

Possible Cause: Failed to read due to mechanical jam;

Possible Solution: Repair mechanical issue.

*901: Slot detected more than nn steps from dest (future)

Meaning of Error: Shuttle position at photometer is not in range to be able to read

Possible Cause: Damage to Comb opto or opto moved

Possible Solution: Re-adjust comb. Contact factory.

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3.9.8 Shuttle Command List

Name Description Explanation Syntax


Vessel mover Moves the Shuttle and the
Cuvette Mover to the home
VHOME (Shuttle & Cuvette VHOME
position
Mover) to home
Shuttle mover Moves the Shuttle to the home
VSHH position. VSHH
home.
Moves the Shuttle to a
position. This value is RMOVE nnnn
Shuttle move to displayed in inches.
VSHM
position Example- to move the
VSHM 300
Shuttle 3.00"
Shuttle mover Displays the current status of
status the Shuttle
SSTAT VShut pos = 299 Shuttle position in inches 2.99 VSTAT
Lastdest = 300 Last destination requested in
state = G [0] 0 inches 3.00
state
Show Shuttle Exercises Shuttle mover full
cycle time stroke 8 times.
STIM STIM
Stime = 17.1 sec Displays a value in
seconds.
Shuttle Moves Shuttle to various
Movement testing positions to test the
mechanical mechanism
VSHTST VShut pos=20, Example shows latest VSHTST
Lastdest=20, positions
state=G[0] Vsh shuttle time in seconds
time = 17.7
Slot Detect Toggles from on to off, with a SLOTDETECT n
(Enable/Disable) 1 or 0
SLOTDETECT
Slot Detect
SLOTDETECT 1
Enable Example
Move Cuvette to Moves cuvette to position,
photo read position read, returns home
READCUV(n,n,n,n
(405/630) [1 / 5] Example reads cuvette row A
Abs = 1.238 [1] position 1, using 405 filter as
READCUV primary and 630 filter as
differential, displays Abs READCUV(A,1,340,405
value REQUIRES OPEN
PARENTHESIS AND
COMMAS
Read 5 Cuvette Reads all 5 cuvette positions, READROW n n n
positions returns home
READROW Requires row (letter A -H),
Read Row A 405
primary filter and differential READROW A 405 630
630 filter
Read 5 cuv pos, with Reads all 5 cuv positions, pos
1 as blank, returns home READROWB n n n
pos 1 as blank
READROWB Requires row (letter A -H),
Read RowB A
primary filter and differential READROWB A 405 630
405 630 filter

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3.10 Chassis
CHASSIS – this section contains Service Instructions. The Chassis consists of a metal base that
serves as the bolting surface for the 4600 components. Several components are considered part
of the Chassis and are called out below. The wiring is not shown but is called out on the list below.
With the exception of the Power supply, wiring, Cable Assemblies, Chassis Fan, and Cooler Board,
most of the components can be serviced or replaced without removing the Chassis (final) from the
Enclosure Base. The Cover does need to be removed to access most of the Chassis parts. See
the Enclosure section for procedures on removing the Cover and Base. Note: As the instrument is
built up with the several sub-assemblies that make the completed instrument, it is still referred to as
the Chassis (final) before it is put in the Enclosure. Some of the references in this section will refer
to the Chassis (final).

The Chassis is designed to give years of trouble free service. There are a few items that may need
attention or replacement during the life of the instrument. They are:

Part Description Part Number


Power Supply part #101110SP
Tubing for Serial Number 330 and below part #135242SP
Tubing for Serial Number 331 and above part #135238SP
Barb, Pipe Fitting for Serial Number 330 and below part #137097SP
Barb, Pipe Fitting for Serial Number 331 and above part #974620SP
Luer, Female part #137102SP
Male Nut part #994651SP
Fuse part #102015SP
Cable, USB 6’ part #104455SP
Cable Assembly, RS1, Ez80, Vmover part #104047SP
Cable Assembly, RS2, Vmover, Photometer, Xmover part #104048SP
Bottle, 250ml) part #187146SP
Bottles and Drain Tubing Kit part #994335
Fuse Board part #994631SP
Motor Controller, X mover part #994633SP
Main Ez80 part #994630SP
Cooler Board part #995577SP
Motor Controller, V mover part #994632SP
Power Cord US part #137521
Power Cord European part #137221
Fan, Chassis part #104508SP

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Chassis (Continued)

Each item is given step by step instructions below.

Motor Controller X Mover Motor Controller V Mover


Fuse Board Power Supply Main Ez80
Cooler Board (hidden)

Prime Bottle Luer Fan Chassis Tubing

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Chassis (Continued)

Chassis (final) Completed instrument without the Enclosure

3.10.1 Power Supply (part #101110SP)


1. To replace the power supply, the Chassis will have to be removed from the Enclosure
Base. See the Enclosure Section for this procedure.

2. The wiring from the power supply is routed under the Chassis. There is a plug that can
be disconnected for much of the wiring. Disconnect this plug and carefully cut the wire
ties that hold the wire harness to the bottom of the Chassis.

Unplug connector, and feed wiring up through this hole after disconnecting from fuse board
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Power Supply (Continued)

3. Un-plug the connectors on the fuse board that come from the power supply and pull
them down through the slot in the Chassis base.

Unplug the power supply connectors from the fuse board

4. Remove the 2 screws that hold the power supply strap, lift the power supply and push
the wire harness up through the hole.

Remove the 2 screws that hold the power supply strap, lift the power supply and
push wire harness up through the hole

5. Remove the ground wire from the old power supply and attach it with the same screw
to the new power supply.

6. Replace the power supply in the reverse order by first feeding the wiring down through
the hole under the power supply.

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Power Supply (Continued)

7. Route the wiring back to the fuse board and plug in connectors to their designated
position.

8. Re-connect the power supply harness plug.

9. Tuck the wiring harness under the power supply and re-mount the supply using the
power supply strap. The power supply front to rear position will need to be re-adjusted
after the Chassis is put back in the Enclosure Base so the power supply strap screws
can remain loose until the final adjustment. Double check that the power supply is not
mounted upside down. See above pictures.
10. Use new wire ties to secure the wiring harness to the bottom of the Chassis. There is
minimal clearance under the Chassis so the harness must be as tight as possible to the
bottom surface.
11. Push the plug back up into the hole with the wiring harness. This should allow the
clearance needed when re-assembled to the Enclosure Base.

3.10.2 Tubing for Serial Number 330 and below (part #135242SP)

1. The tubing acts as the drain system and has a mid-stream “T” to allow additional
venting. To change this tubing, it will be necessary to remove the Wash Cup to gain
access to the bottom of it where the tubing connects. To remove the Wash cup, remove
the 2 Mounting screws in the Wash cup Bracket. There are 2 styles of mounting
brackets used with the Wash Cup.

#994096
Wash Cup
Assembly #994696
Wash Cup for
serial 331 and
above (one
piece)

Remove the Wash Cup bracket mounting screws

2. With the Wash Cup mounting bracket screws removed, the Wash Cup can be lifted and
the tubing pulled off the drain tube in the bottom of the Wash Cup.

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Tubing (Continued)

3. Pull the tubing out from under the Cuvette Mover Deck and out of the hole in the
chassis support bracket.

4. Remove the 2 screws that hold the drain manifold and lift out the manifold.

5. Remove the vent tubing from the wire tie that holds it in position along the right side of
the Shuttle and remove the entire tubing assembly with the drain manifold.

6. Replace the tubing as needed and the Barb, Pipe Fitting in the drain manifold if
needed.

7. Re-install the drain system by routing the new tubing to the Wash cup and connecting it
to the Wash Cup.

Tubing routes to the Wash Cup through this hole in the Chassis Support Bracket

8. Re-install the Wash Cup bracket screws.

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Tubing (Continued)

9. Re-install the drain manifold mounting screws. Make sure the angle on the back
surface of the manifold is facing so it matches the angle on the inside of the Enclosure
Base. If the Chassis has been taken out of the Enclosure Base, these screws can be
left loose until it is reinstalled in the Base. The manifold will need to be adjusted so that
it is tight against the inside of the Enclosure Base.

(95 degree angle)

Check that the angle on the drain manifold looks like this

10. Re-fasten the vent tubing with a wire tie along side of the Shuttle. Make sure the tubing
and wiring do not interfere with the encoder wheel or motor pulley.

3.10.3 Barb, Pipe Fitting for Serial Number 330 and below (part #137097SP)
1. To replace the Barb, Pipe Fitting, the 2 screws that hold the drain manifold will need to
be removed and the manifold removed from the instrument. If the old fitting is broken,
unscrew the broken part from the manifold and tubing.

2. Use a thread sealing compound or Teflon tape on the thread then carefully screw the
fitting into the manifold. The fitting is a tapered NPT (National Pipe Thread) designed
to seal as the taper is threaded into the mating thread. Be careful not to over tighten
this fitting.

Drain Manifold Barb, Pipe Fitting

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Barb, Pipe Fitting for Serial Number 330 and below (Continued)

3. Re-attach the tubing and re-install the manifold to the Chassis. See Section 2, #9 for
correct orientation of the manifold.

135238SP tubing
for Units 331 and
above.

974620SP for
Units 331 and
above.

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3.10.4 Luer, Female (part #137102SP)
1. The Luer, Female fitting is mounted in the Prime Bottle Block Assembly. To replace
this fitting, loosen the screw in the side of the block assembly and remove the block
from the support post.

Loosen screw in side of block assembly Support post

2. Pull the tubing off the bottom of the Luer fitting.

3. Remove the nut on the bottom side of the Luer fitting.

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Luer, Female (part #137102SP) (Continued)

4. Unscrew the fitting from the block.

Pull tubing off bottom of Luer Remove nut on bottom of Luer Unscrew Luer fitting

5. Replace the fitting in reverse order by screwing the new fitting into the block.

6. Screw the nut back onto the Luer fitting.

7. Re-attach the tubing.

8. Slide the mounting block back onto the support post, align and tighten the screw in the
side of the block assembly.

3.10.5 Male Nut (part #994651SP)

1. Remove the black flangeless fitting from the manifold.


2. Change tubing and fitting as needed.

Teflon tubing O-ring Flat Washer Black Flangeless fitting Soft tubing

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3.10.6 Fuse (part #102015SP)
1. The 5 amp fuse is intended to be used in the Fuse Board. In the event of a component
failure, check the status of the fuses on the fuse board and replace as needed. For
further information on troubleshooting fuses, see Section 2.3 Power Subsystem.

3.10.7 Cable, USB 6’ (part #104455SP)


1. The USB cable shipped with the ChemWell®-T is intended to connect the instrument
with a computer. The cable connects between the USB port on the Main board of the
instrument and the USB port on the computer. CAUTION: Only use the cable
supplied with the instrument. The ChemWell®-T has been tested with this specific
cable.

3.10.8 Cable Assembly, RS1, Ez80, Vmover (part #104047SP)


1. The V mover Cable assembly carries data from the main board to the V mover board.
This cable can easily be changed due to the close proximity of the boards. This cable
does not route under the Chassis. Care should be taken when replacing this cable
since it is easy to mis-plug the connector.

3.10.9 Cable Assembly, RS2, Vmover, Photometer, Xmover (part #104048SP)


1. The V mover, Photometer, X mover cable assembly carries data from the main board
via the V mover board to the Photometer and the X mover board. To replace this cable
it will be necessary to remove the Chassis from the Base since the cable is routed on
the underside of the Chassis.

2. The wire ties on the underside of the chassis will need to be cut to release this cable
assembly.

3. Route the new cable assembly in the same manner as the old cable assembly. Special
attention to the Photometer cable is needed since its tight space makes it difficult to
plug it in correctly.

4. Fasten the cable assembly with wire ties when the routing has been completed. Make
sure no wires are in the areas where the feet or center screw will come through the
Enclosure Base.

3.10.10 Bottle, 250ml (part #187146SP)


1. The prime bottle is shipped with the spare parts kit. It holds 250ml which should be
more than enough volume to perform several hundred tests. This part number does not
include the cap with tubing and fittings. That part number is 994601 Cap, Prime Bottle
46.

3.10.11 Bottles and Drain Tubing Kit (part #994335)


1. The Bottles and Drain Tubing kit contain a starter kit of bottles needed for reagents and
samples. The drain kit includes the drain tubing and the “T” fitting that screws into the
back of the instrument or push into the tubing (two styles). Reference Section 3.10.3.

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3.10.12 Fuse Board (part #994631SP)
1. The fuse board contains 8 identical fuses for the various power requirements that
branch off from this board. There is a green LED indicator light behind the fuse that
should be illuminated if the fuse is good. To change a defective fuse, carefully pull the
fuse from the holder and replace with a new fuse. Caution should be observed not to
pry in the center of the fuse as damage could occur to the LED indicator light mounted
on the board behind the fuse. For further information on troubleshooting fuses, see
Section 2.3 Power Subsystem.

3.10.13 Motor Controller, X Mover (part #994633SP)


NOTE: For further information on troubleshooting boards, see Section 2.1 Electronic Hardware.

1. The X Mover Motor Controller board is used to control the Rack Mover and the Probe X
Motors. It also accepts the connectors from the thermistors on the cooling assembly.
To change this board, start by removing the Rack Mover and lifting it out of the way in
order to access the forward X Mover board bracket mounting screw.

X Mover Motor Controller board 2 Bracket Mounting screws (hidden)

2. Remove the 2- X Mover controller board bracket screws.

3. Lift the board up and disconnect the connectors. Do not remove the 4 screws that hold
the board to the mounting brackets.

4. Replace the board in the reverse order by connecting the wiring first.

5. Re-install the 2 mounting screws.

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3.10.14 Main Board Ez80 (part #994630SP)
NOTE: For further information on troubleshooting boards, see Section 2.1 Electronic Hardware.
1. The Main Board Ez80 is located in the right rear of the instrument and is mounted on 2
metal brackets. It is the main processor for the instrument and should show an
illuminated, green indicator (heart beat) light on the top when the instrument is under
power. It also has the USB port on it. To remove this board, start by removing the 2
screws in the mounting brackets.

USB port Mounting Brackets Green indicator light

2. Lift the board up and disconnect the wiring connectors.

3. Replace the board in the reverse order by connecting the wiring first.

4. Re-install the mounting screws in the board brackets and secure to the chassis.

5. Check that the USB port lines up correctly with the Enclosure Base USB opening. It
may be necessary to bend the board brackets slightly to line up the USB port with the
opening.
3.10.15 Cooler Board (part #995577SP)
NOTE: For further information on troubleshooting boards, see Section 2.1 Electronic Hardware.
1. The Cooler Board acts as an interface board for the Cooler Assembly fan motor and the
Peltier. The Cooler Board is located on the Left Hand Probe X bracket. The Chassis
will need to be removed from the Enclosure Base in order to service this board. See
Enclosure section for removal of chassis from base. Begin by unplugging all the
connectors from the Cooler Board.

Cooler board is located on the Left Hand Probe X bracket

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Cooler Board (Continued)

2. Carefully removed the 4 mounting screws and spacers from the Probe X left hand
bracket.

Carefully remove the 4 mounting screws and spacers

3. Re-install the new Cooler board in the reverse order by screwing the board and spacers
back onto the left hand Probe X bracket.

4. Re-connect the wiring.

NOTE: Serial Number 330 and below has a two pin yellow connector from the Peltier to
the cooler board. Serial Number 331 and above, the red and black wires are
screwed into the board.

Serial Number 330 and below has a two pin yellow connector from the Peltier to the cooler board.

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Cooler Board (Continued)

Serial Number 331 and above, the red and black wires are screwed into the board.

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3.10.16 Motor Controller, V mover (part #994632SP)
NOTE: For further information on troubleshooting boards, see Section 2.1 Electronic Hardware.
1. The V Motor controller Board is mounted in the right, rear of the instrument, next to the
Main board. It controls the motors for the Shuttle and the Cuvette Mover. It also
accepts the connector from the thermistor from the Cuvette Mover. To change this
board, first remove the support post directly in front of the board bracket.

V Mover Controller Board Bracket Mounting screws

2. Remove the 2 screws that fasten the board bracket to the Chassis.

3. Lift the board up and disconnect the connectors.

4. Re-install the new board in the reverse order by connecting the wiring connectors first.

5. Re-install the 2 screws that fasten the board bracket to the Chassis.

6. Re-install the support post.

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3.10.17 Power Cord (US = 137521 & European = 137221)
1. Each instrument is shipped with either a US or European Power Cord. Depending on
the country the instrument will be used in, either cord will work equally well. The Power
supply on the ChemWell®-T will automatically adjust to work with voltages from 120
volts to 240 volts AC.

3.10.18 Fan, Chassis (part #104508SP)


1. The Chassis Fan is located under the Photometer and is designed to provide on
demand cooling to the instrument. To change a chassis fan, it will be necessary to
remove the Chassis from the Enclosure Base and the Photometer will also need to be
removed. See Section 4 for Photometer removal and Enclosure section for removal of
Chassis from base. CAUTION: Only use the cable supplied with the instrument.
The ChemWell®-T has been tested with this specific cable.

2. Locate the fan connector on the Main board and disconnect it. Pull the wire to the
underside of the Chassis the cut the wire ties that secure it.

3. Loosen the Power supply strap and slide the Power supply towards the rear of the
instrument. This will allow access to the rear fan screws.

4. Use a short screwdriver to remove the screws from the chassis fan.

Chassis Fan can be changed after the Photometer is removed. Slide Power supply back.

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Fan, Chassis (Continued)

5. Re-install the new fan and guard in the reverse by feeding the wire down the hole in the
chassis and re-installing the fan screws.

6. Re-route the fan wire under the Chassis and up to the Main board. Re-fasten the fan
wire with wire ties in the same manner as it was before.

7. Re-install the Photometer.

8. Re-adjust the Power supply position with the strap bracket. This will need a final
adjustment after the Chassis is put back in the Enclosure Base.

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4. SUB-ASSEMBLY REMOVAL FROM THE CHASSIS

Probe X and Probe Z in Assembled State Photometer Shuttle

Rack Mover Wash Cup Cuvette Mover Diluter

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4.1 Removal of Probe X and Probe Z Assembly
Note: Due to the risk of damage to the Probe Tip, it is recommended to remove the Probe Tip
prior to removing this assembly!

1. To remove the Probe X and Probe Z Assembly, start by removing the Enclosure Top
and Shield. It is not necessary to remove the Enclosure Cover.

2. With the Top and Shield removed, remove the Probe X motor cover. This will require
the removal of 3 screws. There is a ground terminal on one of the screws. Remove the
ground wires from the terminal before removing the screw and terminal. Be careful not
to lose the spacer under the long screw.

Spacer To remove the motor cover, remove the 3 screws and ground terminal.

3. With the motor cover removed, the connectors to the Probe X interface board can be
removed. Unplug the 4 connectors that come out of the wire bundle from the chassis.

4. Unscrew the RED fitting from the rear of the Diluter manifold. The tubing may drip water
out of the end so it may be desirable to allow the water to drain before proceeding.

5. Remove the 2 screws in the Probe X rail bracket that hold the Probe X rail to the
Shuttle bracket on the right side and the Left Hand Probe X bracket on the left. Lift the
Probe X and Probe Z assembly up and away from the instrument. Use caution when
removing these screws as this will allow the Probe X to move or tilt causing
possible damage to the instrument.

Remove the 2 screws in the Probe X rail bracket

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Removal of sub-assemblies from the Chassis (Continued)

6. Re-installation of the Probe X and Probe Z assembly is the reverse of the above
procedure. Start by re-installing the 2 screws in the Probe X rail bracket. The rail
bracket is slotted to allow adjustment of the Probe Tip to the Wash Cup. See the
Alignment section for more information on this procedure.

7. Re-connect the wire connectors to the Probe X interface board.

8. Re-install the motor cover and ground terminal. Re-connect the ground wires to the
ground terminal.

9. Re-install the RED fitting into the rear of the Diluter manifold.

4.2 Removal of Photometer

1. To remove the Photometer, the Enclosure Cover must be removed. See Enclosure
section for cover removal. It is not necessary to remove the Enclosure Base as there
are 2 access holes in the Base for the Photometer mounting screws.

2. Remove the Light Shield by loosening the thumb screw on the side of the Shuttle and
sliding the Shield out and away.

3. Remove the waste bottle bracket cover by removing the 2 screws that hold it to the
chassis base.

Waste Bottle Bracket Cover Remove the 2 screws holding the Waste Bottle Bracket Cover

4. Tilt the instrument up to access the holes in the bottom of the Enclosure Base under
the Photometer. Remove the 2 screws and washers from these holes.

Note: These are special length screws and the same screws and washers must be re-
installed during re-assembly.

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Removal of Photometer (Continued)

Remove the 2 screws and washers from the access holes in the Enclosure Base.

5. Loosen the side screw in the Prime Bottle Block assembly and remove the block from
the support post.

Loosen the side screw in the Prime Bottle Block assembly and remove the block from
the support post.

6. Unscrew the support post that held the Prime Bottle Block assembly and remove.

Remove the Wash Cup support bracket mounting screws and push the Wash Cup to
the side. It is not necessary to disconnect the tubing from the Wash Cup.

Remove the Wash Cup bracket mounting screws

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Removal of Photometer (Continued)

8. Disconnect the yellow lamp connector from the lamp assembly. Pull firmly on the
connector to separate it.

9. Remove the Spacer between the Photometer and the left hand Shuttle bracket by
removing the screw from the back side of the Shuttle bracket. This will completely free
the Photometer from the instrument with the only remaining attachment being the
connectors that come up from below the chassis and plug into the Photometer PCB.

Remove the Spacer between the Photometer and the left hand Shuttle bracket by
removing the screw

10. Carefully slide the Photometer forward and disconnect the ribbon cable and the power
connector from the back side of the Photometer PCB.

11. Continue to move the Photometer forward and remove from the instrument.

Photometer removed (shown out of Enclosure Base for clarity only)

12. Re-installation of the Photometer is the reverse of the above procedure. Make sure to
use the same screws that were removed from the bottom of the Photometer.

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4.3 Removal of Rack Mover
1. To remove the Rack Mover, the Top, Shield and Enclosure Cover will need to be
removed (see Enclosure section).

2. With the Enclosure Cover removed, remove the 4 small flat head screws in the blue
transfer plate and the 4 flat head screws on the top surface at each end of the Rack
Mover deck.

Remove the 4 small flat head screws in the blue transfer plate Remove the 4 flat head
screws on the top surface at
ends

3. Lift the Rack Mover Deck up it will separate from the Cooling assembly. Remove the
ground wire from the Chassis.

Note: With the early machines, serial numbers lower than 250 the cooling assembly
bolts to the chassis with 2 angle brackets (not shown). On these early models, it is
necessary to separate the two units during disassembly. With serial numbers higher
than 250, the cooling assembly can be lifted out with the Rack actuator attached as a
complete unit or you may find it easier just to separate the Cooling assembly as
described above.

4. Move the Rack Mover away from the X Mover Motor Controller board and remove the
screws holding the board brackets to the Chassis. It may be helpful to remove the
support post that is in the left rear corner.

Remove the screws holding the X Mover Motor Controller Board brackets to the
Chassis (screws hidden from view in this example; shown out of Enclosure Base for
clarity).
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Removal of Rack Mover (Continued)

5. Lift the X Mover Motor Controller board up and disconnect the Rack Mover wire
connectors.

7
6
4

8
5

6. The Rack Mover should now be free to lift out of the front of the instrument.

7. Re-installation of the Rack Mover should be the reverse of the above procedure. Be
careful to correctly position the graphite thermal sheet between the bottom side of the
blue transfer plate and the cooling assembly.

4.4 Removal of Cuvette Mover


Note: The Cuvette Mover has been pre-aligned with the Photometer at the Factory.
Do not change or loosen the screws in the Cuvette Mover Mounting blocks.
Remove only the specified screws noted below.

1. To remove the Cuvette Mover, it will be necessary to remove the Enclosure Cover.

2. Remove the 2 flat head screws from the top surface on the front of the Cuvette Mover
deck.

3. Remove the 2 pan head screws from the top surface on the rear of the Cuvette Mover
deck.

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Removal of Cuvette Mover (Continued)

Remove the 2 flat head screws from the top surface


on the front of the Cuvette Mover deck Remove the 2 pan head screws from
the top surface on the rear of the
Cuvette Mover deck

4. Remove the support post in front of the V Mover Motor controller Board.

5. Loosen the 2 screws in the mounting bracket for the V Mover Motor Controller board.

6. Tilt the board forward and disconnect the wiring connectors from the V Mover Motor
Controller board. Disconnect the 2 connectors from the Main Board. Unplug the
ground wire from the chassis.

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Removal of Cuvette Mover (Continued)

7. Carefully lift the front of the Cuvette Mover deck and slide the deck forward while
allowing the rear of the deck to drop down and off the rear support block. Do not rotate
deck while feeding the assembly out from under the Shuttle and past the Cuvette Side
supports. Keep the rear of the deck low until the motor goes under the cut in the rear
Cuvette Side support. Feed the wiring along with the assembly until it is clear of the
instrument. This is one of the more difficult assemblies to remove from the instrument.
Use caution and care when removing this assembly.
8. Re-installation of the Cuvette Mover is the reverse of the above procedure. Feed the
wiring in under the Shuttle first and be careful not to damage the deck or wiring while
re-installing the assembly.

4.5 Removal of Wash Cup


1. To remove the Wash Cup, it will be necessary to remove the Enclosure Cover.

2. With the Enclosure Cover removed, remove the 2 Wash Cup bracket mounting screws.
There are 2 different Wash Cup mounting bracket configurations. See below.

Remove the Wash Cup bracket mounting screws

3. Pull the tubing from the drain tube in the bottom of the Wash Cup.

4. Service as needed and re-install in the reverse order.

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4.6 Removal of Shuttle

1. To remove the Shuttle, it will be necessary to remove the Enclosure Cover. See
Enclosure section.

2. Remove the Probe X and Probe Z assembly as described in Section 1.

3. Remove the Spacer between the Photometer and Shuttle left hand bracket.

Remove the Spacer between the Photometer and Shuttle left hand bracket

4. Remove the support post in front of the V Mover Motor Controller board.

Remove the support post in front of the V Mover Motor Controller board then loosen
the 2 screws in the V Mover Motor controller board mounting bracket

5. Loosen the 2 screws in the V Mover Motor Controller board mounting bracket.

6. Tilt the V Mover Motor Controller board forward and unplug the Shuttle connectors.

7. Remove the 4 pan head screws from the Shuttle right and left hand brackets.

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Removal of Shuttle (Continued)

Remove the 4 pan head screws from the Shuttle right and left hand brackets

8. Lift the Shuttle up and out of the instrument.

9. Re-install the Shuttle in the reverse order of above.

4.7 Removal of Diluter


1. To remove the Diluter, the Enclosure Cover will need to be removed. See Enclosure
section.

2. Unscrew the black fitting from the rear of the Diluter manifold.

3. Remove the 4 pan head screws and ground terminal from the Diluter mounting
brackets.

Remove the 4 pan head screws and ground terminal from the Diluter mounting brackets

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Removal of Diluter (Continued)

4. Lift the Diluter slightly and unplug the USB and power connectors from the PCB located
under the Diluter.

5. Lift Diluter out of instrument.

6. Re-install the Diluter in reverse order of above. Use care when plugging the
connectors back into the PCB as it is easy to plug them incorrectly.

4.8 Troubleshooting Guide for Chassis

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Instrument will not Power not turned on Check for power on the instrument
turn on
Check heart beat light on main board.

Failed power supply Replace power supply

No power at source Check power source.

Failed main board Change main board

Fuse failed Check and replace fuses

Fan will not turn on Fan unplugged Plug fan in

Fan defective Replace fan

Component not Defective fuse Replace fuse


responding

Mechanical jam Repair jam condition

Connector disconnected Check connectors on boards

Motor defective Check and replace motor.

Ribbon cable damaged or


unplugged Replace or plug in

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Troubleshooting Guide for Chassis (Continued)

PROBLEM POSSIBLE CAUSE POSSIBLE SOLUTION


Water on chassis or Broken fitting Replace fitting
dripping out the
bottom
Drain tubing clogged Unclog drain system

Leak in prime bottle tubing Repair or replace tubing

Drain tubing disconnected Reconnect drain tubing

Support posts will Screw backed out Screw back up from bottom of chassis
not tighten
Threads stripped out of post Use proper length or longer screw
Shuttle or Cuvette V Mover controller board Check all connectors
Mover not working

Replace Board

Rack Mover or V Mover controller board Check all connectors


Probe X not working

Replace Board

4.9 Chassis Command List

Name Description Explanation Syntax


Wait time in seconds Default is 1 second WAIT n
WAIT
Waiting 8.0 seconds Example is Waiting for 8 seconds WAIT 8

Wash Probe Default is 400µL, normal operation does not WPRB nnn
WPRB require a number
WPRB 300 Example is washing probe 300µL WPRB 300
Wash probe, rinse
WPRBX WPRBX
before bath
Status info for CW Displays status of photometer and rack
software cooling.
STAT PH-ON [37.0] 36.7 RC- Info displayed, photometer is on, heat STAT
on e[--.-] set to 37.0, current heat 36.7, Rack
cooling on, external temp jack [-- not
plugged in]

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Chassis Command List (Continued)

Name Description Explanation Syntax


STATS Show all brief status info Shows status of the movers STATS
Set or display device
SERN serial number Sern with a number sets the serial number. SERN nn
315 = Serial No Sern displays current serial number SERN 315
RESTART Restart as if power up Bootloader and start up as if power were cycled RESTART
Show firmware revisions Displays current firmware versions and date
VESS: 4600 V 00.15.15 Mar 28 Display V firmware as 00.15.15
2011 10:04:02 V RKPX: Display X firmware as 00.15.15
4600 X 00.15.15 Mar 28 2011 Display Z firmware as 00.15.15
REVS 10:04:07 X PZMX:4600 Z Display P firmware as 00.15.15 REVS
00.15.15 Mar 28 2011
10:04:13 Z PHOT: 4600 P
00.15.15 Mar 28 2011
10:03:52 P
Sets the 4 different parameter for the
PSETVG n,n,n,n
PSETVG Set Vessel Group vessel group in the PLIST
Sets the 4 different parameter for the
PSETWG n,n,n,n
PSETWG Set Wash Group wash group in the PLIST
HELP List all commands All commands listed, some are not available HELP
All movers go home, probe X gets
HOME
HOME Home all movers parked
Automatic activities
IDLE
IDLE control Displays current state, either on or off
IDOF Disable idle events Turns automatic activities off IDOF
IDON Enable idle events Turns automatic activities on IDON
BEEP Beep noise Len, type, volume BEEP

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5. PHOTOMETER CALIBRATION VERIFICATION
The photometer is digital and should never require calibration. If calibration of the photometer is
suspect, exhaust all other possible variables first, such as lamp or dispensing accuracy. Use the
following steps to verify the photometer’s calibration.

5.1 Photometer Calibration Verification Preparation


• Open the Command prompt (F5).
• Send HEATOFF command to ensure that the Cuvette heat is OFF.
• Observe the software response in the communication log.
• Send STAT command.
• Close communications window.
• Select “Cuvettes Heat Off” from the Routines drop down menu. Dialog box will display (see
Figure 5.1-1); type OK to close.
• Verify on the Layout screen that Cuvette Carrier Heat is OFF (see Figure 5.1-1).

Layout
screen

Routine
dialog
window

Figure 5.1-1 Temperature Off

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5.2 4600 Photometer Reference Kit #046034
• Put Photometer Reference Kit in Row A

Figure 5.2-1 Reference glass displayed next to case

Figure 5.2-2 ChemWell®-T Photometer Reference Kit #046034

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Photometer Reference Kit (Continued)

• Open the Command prompt (F5)


• Type: PTVOLTS
• Type: PPTQCAl 405 .3566 (or the number located on the Photometer Master
position #2)
• Type: PTEQ
• Type: PTSAVE F
• Type: Readrow(A,405, -1

Confirm that the Photometer Reference glass readings are within the appropriate range:
• Readings from 0 to 1.5A ± 1%
• Readings from 1.5 to 3.0A ± 2%

Example reading shown below:

If needed, contact the factory for assistance.

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6. ALIGNMENT
After carefully unpacking and hooking up the instrument it is recommended that the following set
up adjustment be performed to verify that the adjustments did not change during shipping. Under
the UTILITIES Menu, the first selection, ALIGNMENT, will lead to another menu of four procedures
to adjust the instrument. They are:

• Probe vs. Wash Cup

• Probe vs. Rack Mover

• Probe vs. Cuvettes

• Photometer vs. Cuvette Carrier

These adjustments should be checked after any shipping or whenever the user suspects a
possible misalignment with the Wash Cup, Rack, or Cuvettes.
All of these alignments have been adjusted at the factory during the testing phase of the machine
but could change as a result of shipping or changing the Probe Tip.
All adjustment windows use similar adjustment buttons. The single arrows are small adjustments
for small moves and the double arrows are larger adjustments for larger moves.
The BLUE buttons adjust the X axis (right and left). Right pointing arrows move X to the right, left
pointing arrows move X to the left.
The RED buttons adjust the Y axis (front to rear). Down pointing arrows move Y towards the front
of instrument, up pointing arrows move Y towards the rear of instrument.
The GREEN buttons adjust the Z axis (up and down). Up pointing arrows move Z up, down
pointing arrows move Z down.

6.1 Probe Vs. Wash Cup


Perform the alignment steps for the Probe to the Wash Cup is to ensure:

• the Probe Tip is level with the top of inner Wash Cup and

• tip moves far enough down into the water to wash the Probe sufficiently.

6.1.1 Using the Alignment Window


When the Probe vs. Wash Cup window is opened, the adjustments allow the user to adjust
the Probe in the X direction (left or right) and in the Z direction (up and down). By clicking
the blue arrows the user can visually center the Probe Tip in the X direction. The Probe
should be adjusted in the Z direction (up and down) by using the green arrows to make the
end of the Probe even with the inner wash well. This is done to achieve the correct
adjustment to the Wash Cup.

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Once the Probe has been adjusted to the Wash cup, run TEST and make sure the Probe
returns to the same position. Click on SAVE before closing the adjustment window. The
Wash Cup can only be adjusted in the Y direction (front to back) with the Cover removed
but most misalignments can be done without removing the Cover.

NOTE: REMOVING THE COVER SHOULD ONLY BE PERFORMED BY QUALIFIED


TECHNICIANS

In the unlikely event that the Probe Tip does not line up correctly in the Y direction, the
Probe X rail is slotted to allow adjustment and the Mixer bracket on the Probe Z can also
be adjusted. If a good alignment has been obtained, SAVE the settings before closing the
alignment window. If adjustment is needed in the Y direction, see the next section.

It is important that the user read and understand the correct method of adjusting the Probe
Tip in the Y direction contained in the following sections. Failure to make this adjustment
correctly could result in poor operation of the instrument.

Adjusting the Probe Tip in the Y Direction:

To adjust the Probe Tip in the Y direction (front to rear), it should first be determined if the
misalignment exists due to the attitude of the Probe Tip (not being straight up and down,
vertical) or if the entire Probe X rail needs to be shifted in the Y direction. Both of these
adjustments may need to be made. A Probe that is not vertical can cause a misalignment
in the Y direction.

Adjusting the Probe Tip to Vertical:


With the adjustment window still open, the user should inspect the angle of the Probe Tip
by observing it from the side of the instrument. The bottom of the Mixer bracket was
adjusted at the factory to be square with the Probe X rail which in turn causes the Probe tip
to be in a vertical condition. This can affect the Y position of the Probe Tip to the Wash
Cup and should not need any additional adjustment. If the Probe Tip does not appear to
be vertical, the bottom of the Mixer bracket to the Probe X rail should be checked with a
small square. To adjust this condition, the Mixer cover will need to be removed. With the
Mixer bracket removed, use a #0 Phillips screwdriver to loosen the 4 mounting screws that
hold the Mixer bracket to the Probe Z Truck.

See Figure 6.1.1-1 for screw locations. Be careful not to change the position of the sheet
metal home flag mounted under the 2 top screws. Once the screws are loose, the Mixer
bracket can be rotated slightly into a square condition to the Probe X rail. As this bracket is
rotated, the Probe moves in the Y axis. Retighten the screws securely since this could
change the adjustment on the Probe Z truck.

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Figure 6.1.1-1

Adjusting the Probe X in the Y direction:

Once the Probe Tip has been adjusted or determined to be in a vertical condition, the
Probe X rail can be shifted in the Y direction to center the Probe tip over the wash well in
the center of the Wash Cup. To access the adjustment screws, the Top must be removed
from the instrument. Once the Top is removed, the 2 screws that hold the Probe X to the
mounting brackets are visible by looking down from the top and on the backside of the
Probe X rail.

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See Figure 6.1.1-2 for screw locations. The slots can be seen under the screws. To shift
the Probe X in the Y direction, the 2 #8 screws must be loosened. A #2 Phillips
screwdriver should be used to loosen and later retighten these screws. With the screws
loosened and the Wash Cup alignment window still open, the Probe X rail should be shifted
either forward or rearward on both sides to center the Probe tip over the Wash cup well.
Both sides should be shifted the same amount. Once the Probe is centered on the wash
well, retighten the 2 mounting screws. Recheck the Probe position over the Wash cup and
readjust if necessary. SAVE before closing the alignment window. This completes this
adjustment and the Wash Cup alignment.

                     Arrows point to screws 

Figure 6.1.1-2 

6.2 Probe vs. Rack Mover


The objective of this adjustment is to align the Probe tip directly over the single drive pin in the
Rack Mover and to adjust the height of the Probe tip to be approximately 1/16” or 1.5 mm
above the pin. This will ensure proper alignment with the various Rack configurations and
ensure that the Probe is allowed to reach the bottom of each bottle or tube.

Using the alignment Window:


When the Probe vs. Rack Mover window is opened, the user can adjust the position of the
Probe tip relative to the single Rack Mover drive pin by adjusting the Probe X position along
with adjusting the Rack Mover position in the Y direction (front to rear). To adjust the Probe tip
to be directly over the single drive pin, use the blue arrows to move the Probe in the X direction
and the red arrows to move the single pin on the Rack Mover in the Y direction. Once the
Probe is centered over the Rack Mover pin, use the green arrows to move the Probe tip in the
Z direction to achieve a distance of approximately 1/16” or 1.5mm over the pin. Once the
Probe tip appears to be directly over the single drive pin and the correct height over the pin, run
TEST to make sure the Probe tip returns to the same position. If it does not return to the same
position, re-adjust as needed to correct the position and continue checking it by running TEST
until it returns to the same position. Make sure to SAVE the adjustment before closing the
window.

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6.3 Probe vs. Cuvettes
The objective of this adjustment is to center the Probe in the Cuvette well in the X and Y
direction (left and right, and also front to rear).

Using the alignment Window:


Insert Cuvettes in rows A and H in the Cuvette Carrier and install the Cuvette Carrier in the
instrument. Open the Probe vs. Cuvettes alignment window and Click OK on the information
window that asks to install the Cuvettes in row A and H. The Probe will move over the Cuvette
Carrier at Row A1. Use caution before bring the Probe down and ensure that it is
approximately over Row A1. Use the blue adjustment arrows in the alignment window to move
the Probe in the X direction (right or left) until it appears to be over the A1 well. Click on the
bottom green button to bring the Probe down. The Probe should be centered in well A1 in the X
direction (right to left). Use the blue arrows to adjust the position as needed until the Probe is
centered in the well. Observe the position of the Probe from the side to see if it is centered in
the well in the Y direction (front to rear). Use the red arrows to adjust the Probe in the Y
direction. The bottom end of the Probe should be within 1/16” or 1.5 mm of the bottom of the
well. Observe the Probe in the Y direction (up and down) and use the green arrows to adjust it
to the correct height. Once the adjustments appear to be correct, run TEST to verify the Probe
positions correctly in the wells and is sufficiently deep enough in the wells to mix correctly.
Once the settings are correct, click on SAVE before closing the window.

6.4 Photometer vs. Cuvettes


The objective of this adjustment is to ensure the Cuvettes transfer smoothly from the Cuvette
Carrier to the Photometer and back into the Cuvette Carrier without any resistance or clunking.

Using the alignment Window:

Insert Cuvettes in rows A and H in the Cuvette Carrier and install the Cuvette Carrier in the
instrument. Open the Photometer vs. Cuvettes alignment window and Click OK on the
information window that asks to install the Cuvettes in row A and H. The Cuvette Carrier will
move to position A and stop. There are only red arrows in the adjustment window to allow
adjustment in the Y direction (front to rear). Before running the TEST, the alignment of the
forward edge of row A should be observed from above to ensure it lines up with the forward
edge of the Transfer block on the Photometer. The Cuvette can be moved in the X direction
(from the Cuvette Carrier to the Photometer) to check for free and easy movement. Use the
red arrows to move the Cuvette Carrier into alignment with the Photometer as needed. Run
TEST to check that there is smooth transfer of the Cuvette from the Cuvette Carrier to the
Photometer with no resistance or clunking. This is a very critical adjustment and failing to
adjust it correctly can result in mechanism jams. There are special tools available, please see
Appendix C.

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7. CONTACT INFORMATION

If you continue to have problems after consulting your dealer, contact the factory.

Phone: USA 772-283-6540


Fax: USA 772-283-8020
Email: chemwell-support@awaretech.com
Mailing address: Awareness Technology Inc.
P.O. Box 1679
Palm City, FL 34991
USA

ƒ When contacting us, please have the serial number of the ChemWell®-T in question
ƒ Have a description of the problem with as much detail as possible.
ƒ Save any relevant jobs or logs to disk and send or email us the information.

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restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
APPENDIX A – FUNCTIONAL/WIRING BLOCK DIAGRAM

218 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011
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restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
APPENDIX B – CHEMWELL-T COMMAND LIST
NOTE: Some commands may require a password.
1 BEEP Beep Noise
2 BLPR Backlash_PushReserve
3 CALEDGE Find/Check Cal Strip Edges
4 COMMHISTORY Show history
5 COOLMAXINT Reagent cooling max int temp
6 COOLMINRACK Reagent cooling min rack temp
7 COOLOFF Reagent cooling off
8 COOLON Reagent cooling on
9 COOLTEMP Reagent cooling temperature display
10 DASP Diluter aspirate a volume µL
11 DDSP Diluter dispense a volume µL
12 DDUMP Diluter dump settings
13 DINI Diluter initialize
14 DLIST Diluter list settings
15 DLOAD Diluter load settings
16 DMAP Diluter make air pocket
17 DPRI Diluter prime
18 DSAVE Diluter save settings
19 DSETDEFAULT Set diluter to defaults
20 EXTTEMP Read Temp from External Probe
21 EXTTEMPTRIM Set trim for ext temp port
22 FCE Find cuvette edge
23 FWMOFF Photometer filter motor off
24 FWMON Photometer filter motor on
25 FWMSPD Photometer filter motor speed
26 GETFILT List unit filters
27 GOCUV Move probe to cuvette position
28 GOCUVASP Move probe to cuvette position and aspirate
29 GOCUVASPML Aspirate from rack position
30 GOCUVDSP Move probe to cuvette position and dispense
31 GOCUVDSPML Dispense at rack position
32 GOCUVDSPMXML Dispense at cuvette position, plus mix
33 GOCUVML Move vessel and probe to position
34 GOCUVRDYNWML Move Vrack to read Yposition, no wait
35 GOCUVYNWML Move Vrack to Yposition, no wait
36 GOPRIMASP Bring diluent from prime bottle

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restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
ChemWell-T Command List (Continued)

37 GORACK Move probe to rack position


38 GORACKASP Move probe to rack position and aspirate
39 GORACKASPML Aspirate from rack position
40 GORACKDSP Move probe to rack position and dispense
41 GORACKDSPML Dispense at rack position
42 GORACKDSPMXML Dispense at rack position, plus mix
43 GORACKML Move rack and probe to position
44 GORACKYNWML Move Rrack to Yposition, no wait
45 HEATCTL Heat control method
46 HEATOFF Reaction heat off
47 HEATON Reaction heat on
48 HEATSET Reaction heat set
49 HEATTEMP Reaction temp display
50 HEATTRIM Reaction heat detail set
51 HELP List all commands
52 HOME Home all movers
53 IDLE Automatic activities control
54 IDOF Disable idle events
55 IDON Enable idle events
56 LPFANOFF Lamp fan off
57 LPFANON Lamp fan on
58 LPOFF Lamp off
59 LPON Lamp on
60 MCHANGEID Change module ID
61 MIX Mix at current position
62 MIXATRACK Mix at rack position
63 MODVERS List module versions
64 MTDUMP Mover trim dump settings
65 MTHOME Mover home
66 MTINIT Mover reinitialize
67 MTLIST Mover trim list settings
68 MTMOVE Mover move
69 MTMOVENW Mover move (no wait)
70 MTSAVE Mover trim save settings
71 MTSETHG MT set hdw group
72 MTSETPG MT set PID group
73 MTSETSG MT set Spd group

220 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011
©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
ChemWell-T Command List (Continued)

74 MTTIM Mover stroke timing


75 MTTST Mover stroke testing
76 MUPDATEFW Update module firmware
77 MXOFF Turns off probe mix
78 MXON Turns on probe mix
79 MXSPD Sets probe mix speed
80 PARK Park probe at wash cup
81 PCNT Probe current sense reading
82 PDUMP Export settings
83 PDUMPALL Export ALL settings
84 PFFR Probe Z move down until fluid is sensed
85 PINIT Photometer initialize
86 PLIST List settings
87 PLOAD Load settings
88 PLVL Set plate dispense offset
89 POSI Show all brief status info
90 PRXH Probe X to home
91 PRXM Probe X to position
92 PRXTST Probe X movement testing
93 PRZH Probe Z to home
94 PRZM Probe Z to position
95 PRZTST Probe Z movement testing
96 PSAVE Save settings
97 PSEN Probe sensitivity setting
98 PSETAG Set sound group
99 PSETCG Set Cool group (Cool Max temp, Heat Max Temp, cont)
100 PSETDEFAULT Set unit to factory defaults
101 PSETHG Set Heat group (Heat Temp,Heat Trim Temp, cont)
102 PSETLG Set Lamp group (Warmup,Max Idle, cont)
103 PSETMG Set Mix group (mix speed,mix height, mix time)
104 PSETPG Set Probe group (max dip,min dip, max air plug)
105 PSETRG Set Reagent group (prx,rky,pmz)
106 PSETSG Set Shuttle group (rky,shx)
107 PSETVG Set Vessel group (prx,rky,pmz,pdz)
108 PSETWG Set Wash group (prxw,prxd,przF,przB)
109 PSTAT Photometer status
110 PTDUMP Photometer Dump settings
111 PTEQ Equalize ampl/offsets

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restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
ChemWell-T Command List (Continued)

112 PTLIST Photometer list settings


113 PTLOAD Photometer load settings
114 PTQCAL Set ramp to make A2 match target
115 PTSAVE Photometer Save settings
116 PTSETAG MT Set ampl group
117 PTSETCG MT Set cal group
118 PTSETFG MT Set perm group
119 PTSETHG MT Set hdw group
120 PTSETOG MT Set offs group
121 PTSETTG MT Set tune group
122 PTSETWG MT Set wave group
123 PTUNITCAL Adjust unit calibration
124 PTVOLTS Show Gain x1 volts
125 PWSH Probe wash set volume uL
126 QDIL Query diluter
127 RABS Photometer abs
128 RABSA Photometer abs (-air)
129 RAIR Photometer takes air readings
130 RCOUNTS Photometer read in counts
131 RDADC Display ADC chan from device
132 READCUV Move vessel to photo read position
133 READCUVML Move to and read cuvette
134 READCUVNHML Move to, read cuvette & no return
135 READROW Read all 5 cuvette positions
136 READROWB Read all 5 (position 1 as blank)
137 RESTART Restart as if power up
138 REVS Show firmware revisions
139 RHOME Rack to home
140 RINIT Rack mover initialize
141 RMOVE Rack move to position
142 RQ ID request from device
143 RQALL ID request from all device
144 RQTM ID request from device
145 RRKTST Rrack movement testing
146 RSTAT Rack status
147 RTIM Show Rack cycle time
148 RUNMODE Set unit run behavior

222 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011
©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
ChemWell-T Command List (Continued)

149 RVOLTS Photometer volts


150 SAIR Set air gap parameter uL
151 SCANCUV Scan cuvette
152 SERN Set device serial number
153 SLOTDETECT Slot detect enable
154 STAT Status info for CW-T software
155 STATLIST Mover status list
156 STATS Show all brief status info
157 STIM Show Shuttle cycle time
158 VER Show firmware rtrevisions
159 VERBOSE Display extra info
160 VHOME Cuvette mover & Shuttle to home
161 VINIT Vessel mover initialize
162 VRKM Vessel rack to position
163 VRKTST VRack movement testing
164 VSHH Vessel shuttle to home
165 VSHM Vessel shuttle to position
166 VSHTST VShuttle movement testing
167 VSTAT Vessel mover status
168 VTIM Show V cycle time
169 WAIT Wait time in seconds
170 WPRB Wash probe
171 WPRBX Wash probe (rinse before bath)
172 XINIT Probe X initialize
173 XLIST Evaluate Xref values
174 XREF Refresh Probe Xref
175 XREFTST X Ref test
176 XSTAT Probe X current status
177 XTIM Show Probe X cycle time
178 ZINIT Probe Z mover initialize
179 ZSTAT Probe Z status
180 ZTIM Show Probe Z cycle time

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©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.
APPENDIX C – REPLACEMENT PARTS

Contact Awareness Technology Inc. for the latest spare parts list and pricing.

224 ChemWell®-T Project 4600 Service Manual Doc. 4600 Rev. A 11/2011
©Awareness Technology Inc. Unauthorized duplication is strictly prohibited. Information given in this manual is
restricted for use by authorized personnel and is to be used for the sole purpose of providing routine instrument
maintenance and repair services.

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