Galileo Echo

Service Manual

Chapter 3
System Installation
2008-04 Revision 2

Galileo Echo - Service Manual - Chapter 3

ImmucorGamma Europe – SOP Euro.319 & SOP Euro.320
Level 4 – Form Euro 323 – Rev:2
 Table of Contents

Table of Contents

1 System Installation 1

1.1 Setting up the System 1

1.1.1 Tools 1
1.1.2 Procedure 2
1.1.3 Switching on the machine 10

1.2 Post Installation Checks (Qualification) 12

1.2.1 Robot (Strip transfer) 12

1.2.2 Incubator 12
1.2.3 Centrifuge 13
1.2.4 Camera Reader 14
1.2.5 Probe assembly and fluidics 19
1.2.6 Washer 21
1.2.7 Stirrer mechanism 22

1.3 Checks with the Application Software 23

1.3.1 Loading Assay Files 23

1.3.2 Instrument initialization 23
1.3.3 Incubators display 24
1.3.4 Verification of barcode reading 24
1.3.5 Version Verification and Backup 25
1.3.6 Debugging tool verification 25

2 System Installation 26

2.1 Preparation 27

2.2 Procedure 27

Galileo Echo - Service Manual - Chapter 3

ImmucorGamma Europe – SOP Euro.319 & SOP Euro.320
Level 4 – Form Euro 323 – Rev:2
1 System Installation

The Galileo Echo contains delicate electrical and mechanical

components. It must be unpacked and installed by a qualified service
engineer or application specialist.

1.1 Setting up the System

Do not position the equipment so that it is difficult to disconnect the

power supply
To ensure trouble free running of the Galileo Echo system, it is
important to locate the analyser in an appropriate environment.

The dimensions of the Galileo Echo system are:

Main instrument Fluidics module

Width: 28in (67cm) 17in (43cm)
Depth: 19.5in (44cm) 23in (57cm)
Height: 19in (48cm) 13.5in (34cm)
Weight: 84lbs (38kg) 20lbs (9kg)

The Galileo Echo system (Main instrument, fluids module, power

supply and PC) must be placed on a work bench that fits with the
size of the instrument in the laboratory. The bench must be strong
enough to support the weight of all the components and must be a
firm and level surface.

Adequate space should be provided for accessing the system,

both during operation and in case of servicing, a gap of at least 10
cm is required behind the analyser for ventilation.

1.1.1 Tools

The following equipment is required to unpack and set up the

instrument.

o Installation Qualification Document.

o American Standard hex wrench (Allen key) set .050”-3/16”, long
shaft ball end preferred
o Metric hex wrench (Allen key) set, long shaft ball end preferred

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ImmucorGamma Europe – SOP Euro.319 & SOP Euro.320
Level 4 – Form Euro 323 – Rev:2
 o 3/16”, 1/4”, 5/16”, 11/32”, 3/8”, 7/16” nut drivers (set with small
outside diameter will be most useful)
o 3/16”, 1/4", 5/16”, 11/32”, 3/8”, 7/16” open end wrenches (ignition
wrenches)
o Adjustable wrench 0-.94”
o Small channel lock pliers that will open to 1”
o Needle nose pliers w/ teeth
o Wire cutters
o Philips screw drivers #1 and #2, variety of lengths, one #2 w/
minimum 6” shaft
o Slotted screw drivers, variety for screws from #2 to 5/16-18
o Scissors/cutter
o Small weighing scale, Immucor part #0005996
o Tachometer, Immucor part #0005997
o Tempa-DOT thermometers, Immucor part #0087065
o 6 empty 10mL vials, and 7 empty test tubes, supplied with the
instrument

1.1.2 Procedure

The instrument comes in three crates (cardboard packages on one

pallet base), according to the packing list:

o Main Instrument
o Fluids Module and Instrument top cover
o Accessories

It is accompanied by five boxes containing PC and accessories.

1. Unpack the main instrument as follows:

a. Remove the smaller cardboard box from the delivery
package of the main instrument.
b. Open it and remove the protective cardboard frame.
c. Lift the instrument, using two handlers positioned on the
left and right sides of the unit.

Take care – 2 people are required for this step!

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Level 4 – Form Euro 323 – Rev:2
 Figure 1-1: Main instrument shipping assembly

2. Place the instrument on the work bench on its back (rear side)
and remove the 7 screws (Flat head) which hold the wood
shipping frame in place. Then stand the instrument on its feet.
3. Remove the 5 shipping brackets (Fixed with 5/32 hex screws).

Figure 1-2: Shipping brackets (Robot arm)

Figure 1-3: Shipping brackets (Probe arm)

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ImmucorGamma Europe – SOP Euro.319 & SOP Euro.320
Level 4 – Form Euro 323 – Rev:2
 Figure 1-4: Shipping bracket (Stirrer mechanism)

4. Remove the 3 hex screws which fix the centrifuge on the

instrument chassis (2 at the front and 1 at the rear of the
centrifuge module).

Figure 1-5: Centrifuge shipping screws

5. Unpack the fluidics unit as follows:

a. Remove the smaller cardboard box from the delivery

package of the fluids module.
b. Open it and remove the protective material.
c. Take the top cover away and put it in a safe place (Do not
fit the cover at this stage).
d. Lift the fluids module and remove the 5 hex screws
holding the wood shipping frame in place.

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Level 4 – Form Euro 323 – Rev:2
 NOTES:
PROTECT 3 EDGES
1 PART OF SHIPPING ASBY 8063018.
W/BUBBLE WRAP

19111 5X PACK BUBBLE WRAP

16068 5X ABOVE 8060575 AND 1
(0 SHOWN) BETWEEN IT AND 8060546
(7112197 )
8X

(8060575) SEE STEP 3

1
(806225 8)

SEE STEP 2

48255 (8060546)
2X

47010
2X
HELD IN PLACE
WITH TAPE. 48255
DO NOT REMOVE 2 SIDES
ADHESIVE RELEASE
PAPER FROM THE FOAM

S TE P 1

1
(8062259)

S TE P 3
TAPE BOX SHUT

SEE STEP 1

1
(8062257)
1
1 (8062256)
(8062261)
ORIENT WITH
TEXT SHOWN
HERE.

S TE P 4
TAPE BOX SHUT

BI O -TEK

S TE P 2 FLUIDICS UNIT
SHIPPING ASBY

8 0 6 0 5 9 0 -AS

Figure 1-6: Fluidics unit shipping assembly

6. Place the fluids module on the bench to the left of the main
instrument.
7. Unpack the accessories. The box contains the following parts:

o Waste, shuttle and supply bottles (and support frame)

o Power supply
o Tubing and data cables
o Probe assembly
o Sample and reagent racks
o Trays and strip holders
o Camera drivers CD and instrument configuration CD
A B C D E F G H I J

NOTES:

.1 PA R T O F S H IP P IN G S E T 8 0 6 3 0 2 0
10 PLAC E 8063014, 8060708,
8063017, 8060002,
75034 & PO W ER C O R D
8060578 SEE BOM O N T O P. 8062104
2X (P O W E R S U P P LY )
W R AP BU BBLE W R AP

1
(8 0 6 2 2 9 8 )
9

8063016

8060576

)(C A M E R A
(8 0 6 2 2 9 3 )
1

8060594
X
5
8062252
(1 S H O W N )
7 21X
(4 S H O W N )

8060706

8060577

(8 0 6 2 2 9 4 )
1 X
5
6

8060593
4X
(1 S H O W N )

(8 0 6 2 2 9 3 )
1 X5
8060592 4X
5 X0 6 0 7 0 3 4
8
8060704 1X
(2 S H O W N )

(8 0 6 2 2 9 5 )
1

1
(8 0 6 2 2 9 6 ) SEE STEP 1

STEP 1 2 STEP 2
A F T E R A L L T H E PA R T S A R E
IN T H E B O X . P L A C E B U B B L E
W R A P O N T O P T O F IL L G A P.
TA P E B O X S H U T.
40920
I CHG8060565TOSEEBOM
H REMOVE(97168)ADD(CAMERA) 40723
12/5/0
B6
PS
8/17/06 BPS
IN T E R P R E T IAW A N S I Y 1 4 .5 - 8 2
B IO -T E K
H IG H L A N D PA R K , B O X 9 9 8
G REMOVE97165,ADD97168 40540 5/4/06 DJV UNLESS O T HERWISE SPECIF IED APPRO VALS DAT E W IN O O S K I, V T. 0 5 4 0 4 - 0 9 9 8
D RA W N
A L L D IM E N S IO N S A R E IN IN C H E S B.SMITH 7/28/05
F CHGPACKAGING 40354 3/3/06 DJV P R O D U C T IO N TO L E R A N C E S
A PPRO V .
.X .0 1 5 A N G U L A R TO L . 2 B.SMITH 11/14/05
ADD 75034 CABLE IN STEP 2 40262 1/31/06 TI TLE :
E BPS .X X .0 1 0
S U R FA C E F IN IS H 6 3 TH IS D OC U M EN T C ON TAIN S C ON FID EN TAL
.X X X .0 0 5
AN D PR OPR IETER Y IN FOR MATION OF
D ADD RACKS 40086 12/13/05 BPS M AT ER IAL
1 BIO-TEK IN STR U MEN TS IN C . AN D N IETH ER

C ADD 97165; MOVE 8063016 40060 11/1/05 BPS

- IT N OR TH E IN FOR MATION S C ON TAIN ED
THERE IN SHALL BE DISCLOSED TO OTHERS
S H IP P IN G A C C E S S O R IE S
OR DUPLICATED OR USED FOR OTHERS
F IN ISH WITHOUT EXPRESS WRITTEN CONSENT
B ADD 8060002 40032 10/13/05 BPS - OF BIO-TEK INSTRUMENTS INC. SI ZE D WG . N O . RE V .
A RELEASE T O PRO DUCT IO N 39916 9/13/05 BPS
T H IR D AN GLE
D 8060001-A S I
REV. DESCRIPT ION ECO DATE BY DO NO T S CA LE BR EAK SH AR P
ED GES
R EM OVE
ALL BU R R S
PROJECT ION SC A LE : 1/1 - SH E E T 1 OF 1

Figure 1-7: Accessories shipping assembly

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Level 4 – Form Euro 323 – Rev:2
 8. Connect the two components together with the supply tubing and
waste tubing according to the scheme located on the back panel
of the fluidics module. Be sure that the tubing is connected to the
correct orientation (e.g. top to top, bottom to bottom, left to left
and right to right). Also, be sure to connect the probe supply
tubing by screwing it into the back of the Fluidics module and the
back of the instrument.
9. Connect the two components together with the power and data
cables.

P R O BE S U P P LY

R IN S E S TATIO N S U P P LY

R IN S E S TATIO N W A S TE

M A N IFO LD W A S TE

W A S H E R M A N IFO LD S U P P LY

Figure 1-8: Main instrument connections

10. Install the probe.

S TE P 1
S TE P 6

S TE P 3

HA NDL E THE P RO B E S TE P 5
CA RE FUL LY.
DO NO T K INK THE S TE P 2
TE FL O N TUB ING
O R B E ND THE S TE E L . S TE P 4

PR OBE IN STAL L ATION IN STR U C TION S

PR OBE IN STAL L ATION IN STR U C TION S
STEP 1 . IN STAL L PR O BE C OIL BR AC KET U N D ER
STEP 5 . C L IP PR OBE TU BIN G IN TO C L IP AS SH OWN .
R EAR WAL L . AL IGN IT TO TH E PIN S.
STEP 6 . C AR EFU L LY R EM OVE TH E PL ASTIC C OVER FR OM
STEP 2 . IN STAL L TH E 1 ST TU BIN G BR AC KET AS SH OWN
TH E FITTIN G BY GEN TLY WIGGL IN G IT FR OM SID E
TO SID E AN D SC R EW TH E FITTIN G IN TO TH E BL OC K
STEP 3 . R EM OVE PL ASTIC C OVER FR OM PR OBE TIP TAKIN G
ON TH E R EAR WAL L BEL OW TH E PR OBE C OIL
C AR E N OT TO BEN D TH E PR OBE AN D C AR EFU L LY IN SER T
(STEP 1 ) WH EN C OM PL E TE, TH E TEFL ON TU B IN G
TH E PR OBE IN TO TH E PR OBE H OU SIN G U N TIL IT SN APS
SH OU L D FOR M GEN TL E L OOPS WITH N O C R OSSIN G
IN TO PL AC E. TH E PR OBE M U ST BE PU SH ED PAST SOM E
OVER OR TWISTIN G BETWEEN SEC TION S.
R ESISTAN C E U N TIL IT D ETEN TS IN TO PL AC E. N O M ETAL
SH OU L D BE VISIBL E AT TH E TOP OF TH E EN TR AN C E H OL E.

STEP 4 . IN STAL L TH E 2 N D TU BIN G BR AC KET AS SH OWN .

Figure 1-9: Instrument probe installation

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ImmucorGamma Europe – SOP Euro.319 & SOP Euro.320
Level 4 – Form Euro 323 – Rev:2
 11. Place a strip holder that contains two CMT strips with barcode
IDs (prime holder) at the wash prime station (above the wash
manifold support).
12. Place the power supply device in a convenient location and
connect the power supply to the instrument.
13. Connect the power supply to the UPS provided.

The PC and its components come in five boxes:

o Personal Computer
o Touch Screen monitor
o Printer
o UPS
o Hand scanner and additional accessories

1. Unpack the computer and place on counter top.

a. Combine the keyboard cable and handheld scanner and
connect to the back of the computer.
b. Connect the mouse and power cord into the back of the
computer.
c. Do not plug power cord into power source.
d. Check that the supply voltage setting of the computer (Red
Selector near the power connector on the back) is set for the
correct mains voltage (230 volts for EU/110 volts for US).
2. Unpack the monitor and place on top of the Fluidics module.
a. Plug USB cable and blue monitor cable into monitor and the
back of the computer.
b. Connect the power cord into the monitor, but do not plug into
power source.
3. Unpack the printer and place on the counter near the computer.
a. Plug USB cable into printer and the back of the computer.
b. Plug the power cord into the printer, but not into the power
source.
4. Once all cables are plugged into all computer components, plug
the power cords into the power source and turn on all
components.
a. Unpack the UPS and place on the counter.
i. Remove the front cover and verify the battery is
connected.
ii. Plug the UPS into a designated power outlet.

The printer should not be plugged into the provided UPS. It

should be plugged directly into a wall outlet.

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Level 4 – Form Euro 323 – Rev:2
 Serial Cable

Handheld scanner and

keyboard

Mouse

Monitor
USB Cables for
Monitor, Camera
and printer

Figure 1-10: PC cable configuration

5. Insert the install disk into the DVD drive.

a. Double click on the “Setup.exe” file to load the G3Utility.
Confirm the installation prompts.
i. Go to folder C:\Program
Files\Biotek\Immucor G3Utility and create
a shortcut of the file G3Utility.exe.
ii. Move the shortcut to the Desktop.
b. Return to the D:\ drive and double click on the “Echo
Install.exe”.
i. Select the market specific version and press O OKK.
Press O OKK to begin installation.
ii. Press O OKK and Close when installation is complete.
c. From the installation CD open the printer folder and double
clicking on the “Setup.exe” file. Select the 1022n printer and
follow the installation prompts on the screen.
i. When prompted, connect the USB to the back of the
computer.
ii. Select C Coonnttiinnuuee AAnnyyw
waayy.
iii. When prompted to insert disk, click O OKK and then click
the BBrroow
wssee button. Choose the file path
D:\PrinterDriver and click O Oppeenn and then click
OOKK.
iv. Uncheck the Register box and click FFiinniisshh.

If the correct Printer driver is not available on the installation disk, use
the printer driver disk that was provided with the printer.

d. Remove the install CD from the DVD drive.

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Level 4 – Form Euro 323 – Rev:2
 6. Load the monitor driver CD and confirm the installation directions
on the screen. Select the USB driver from the driver options
menu.
7. Restart the computer.
8. Press F10 when the HP logo appears in order to enter BIOS.
a. Select English and press the Enter key
b. Go to Advanced and select Power-on Options.
c. Disable the F9 prompt line, the F10 prompt line, and the F12
prompt line by pressing the right arrow key at each selected
line.
d. Press F10 to accept the changes
e. Go to Security and select Setup Password.
f. Enter the password OCHE, press the Tab key and re-enter
the password.
g. Press F10 to accept the changes.
h. Go to File and select Save Changes and Exit. Press the
Enter key and press F10 to confirm. At this point the
computer will re-boot.
9. Program the handheld scanner with the provided programming
sheet.
10. Set the date/time and Daylight Saving Time rule.
a. Go to the control panel and double click key on Date and
Time.
b. Click on the Time Zone tab and select the appropriate time
zone for the facility.
c. For geographic areas that do not follow Daylight Savings
Time, uncheck the prompt „Automatically adjust clock for
daylight savings changes‟.
d. Return to the Date & Time tab and adjust the time if
incorrect.
11. Plug the serial cable and the USB cable from the instrument into
the back of the computer.
12. When the USB cable is plugged in, the Found New Hardware
wizard will appear.

a. Select No, not at this time and press the N

Neexxtt button.

b. Select Install the software automatically and press N

Neexxtt..

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Level 4 – Form Euro 323 – Rev:2
 c. Press C
Coonnttiinnuuee AAnnyyw
waayy.

d. Press FFiinniisshh. The camera driver is now installed and ready to

be used.

1.1.3 Switching on the machine

1. Power on the instrument.

2. From the desktop, execute the G3 Utility software and open the
C:\ICL Files\IQ-PM folder.
3. Execute the UPLOAD AUTOCAL-CONFIG.ICL file (Click the R Ruunn
FFiillee button in the G3 utility software). This script uploads the
Autocal and Config parameters from the controllers to the PC.
4. Check the Step box in the G3 utility software and click R Ruunn
CCoom mmmaanndd for each step. Verify that the command output has a
status of 0 for each step.
5. Verify that the serial numbers in the command output box match
the serial numbers on the side of the instrument and fluidics
module.

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Level 4 – Form Euro 323 – Rev:2
 Figure 1-11: Upload Autocal and Config output

6. Print screen the window.

7. Open the Vessel Config from the Configuration → Fluidics
menu
8. Verify that the „Microliter per Step‟ values are set to 13 for vessel
numbers 4, 5, 8 and 9 (See Figure 1-12 below).

Figure 1-12: Fluidics Board Vessel Config Data

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Level 4 – Form Euro 323 – Rev:2
 9. If the values are wrong, copy the correct Config data files (Refer
to ETAN-2007-01: Galileo Echo „Config Data‟ files upgrade) and
download the configuration again to the controllers by executing
the DOWNLOAD CONFIG DATA.ICL file.
10. Open the STARTUP.ICL file. Click the R Ruunn FFiillee button in the G3
utility software.

1.2 Post Installation Checks (Qualification)

For the Post Installation Checks you will need to use the G3 Utility
software and ICL files (See chapter 6 'Alignments and Calibrations'
for more details on how to use G3 Utility and ICL files). Sections of
this chapter contain instructions for the verification of mechanical
movements and settings of the Galileo Echo components.

If a command line is blocked or a file is aborted, click on the

AAbboorrtt button and then run the STARTUP.ICL file before
continuing.

1.2.1 Robot (Strip transfer)

1. Place two strips in a strip holder and place it in position 1 of

ambient incubator tray 1. Load three empty strip trays in the
remaining positions of the ambient incubator.
2. Remove the wash prime holder
3. Run the ROBOT AUTOCAL VERIFY.ICL file.
4. Verify that all alignments are correct and that the robot transfers
the strip holder correctly.
5. If adjustments required refer to: chapter 6 'Alignments and
Calibrations – section 1.2.2 Autocalibration'.
6. Replace the wash prime holder on its station.
Shake 7. Place a strip holder in position 1 of tray 1 with a piece of
parameters reflective paper on top of some electric tape near the middle of
the strip holder.
8. Run the TEST SHAKE.ICL file.
9. With a tachometer, observe that the performance is as expected.
10. Record the measured value and compare with acceptable range
in IQ form.

1.2.2 Incubator

1. Place two strips plus one temperature strip in each of 3 strip

holders.
2. Put them in ambient incubator tray 1 from position 1 to 3: 1 for
top still air; 2 for forced air and 3 for bottom still air chambers.
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Level 4 – Form Euro 323 – Rev:2
 3. Run the LOAD INCUBATOR.ICL file.
4. Wait at least 20 minutes for the strips to come at expected
temperature. Note that you can perform other IQ tasks during
this lapse of time.
5. Run the UNLOAD INCUBATOR.ICL file
6. After the strip holders are returned into the tray; record the
temperature displayed by the temperature strips in the
corresponding sections of the IQ form. Check that the measured
temperatures are within the acceptable range defined in the IQ
form.
7. Log the Tempa-dot serial number and expiration date on the IQ
form.

1.2.3 Centrifuge

1. Place a strip holder with two strips and a piece of reflective paper
stuck on the front of the holder in ambient incubator tray 1
position 1.
2. Open the centrifuge service door

Figure 1-13: Centrifuge service door opened

3. Run the CENTRIFUGE TEST.ICL file.

4. With a tachometer verify that the centrifuge is spinning at the
following speeds:
o 1000rpm, 1500rpm, 2300rpm and 2800rpm
5. Record the spinning speed values in the IQ form. Check that
they are within the acceptable range as defined in the IQ form.
6. Open the CALIBRATE CENTRIFUGE.ICL file. Verify that the
Step box is checked. Select line 3 (HOME AXIS) and press the
RRuunn C
Coom
mm maanndd button through line 6 (GET_CENT_CAL). Do not
press R Ruunn C
Coommm maanndd for line 7 (CENTRIFUGE_CALIBRATE).
7. From the Command Output window, record the Centrifuge Home
Offset (CENTC value) in the IQ form and label the centrifuge with
the CENTC value.

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Level 4 – Form Euro 323 – Rev:2
 1.2.4 Camera Reader

1. Select the Diagnostics menu to open the Camera dialog box

Figure 1-14: Diagnostic menu: Camera

2. Place a strip holder with two strips in ambient incubator position

1 of tray 1. Mark X‟s in the bottom of each well in both strips and
mark X‟s in the top of the strip tabs.
3. Open the CAMERA ALIGN.ICL file.
4. Check the Step box and click R Ruunn C
Coom
mmmaanndd button until the first
„POSITION_AXIS‟ command line is highlighted in the ICL
commands display.
5. Click on the C Coolloorr SSeettttiinnggss button (and on the M Moorree…
… button the
first time only) to open the color settings dialog.

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 1. Automatic
Gain Mode

2. Shutter Speed

5. Manual Gain
Control
4. Auto WB Tracking
Off

3. Color Level

Figure 1-15: Camera Control: Color Settings

6. Under the “Automatic Gain Mode”, click on the scroll control to

turn the AGC Off.
7. Verify the “Shutter Speed Control” is set to 1/60th.
8. Adjust the “Color Level” to 75%.
9. After the color of the image has stabilized, uncheck the “Auto WB
Tracking” box.
10. Use the “Manual Gain Control” to fine tune the brightness level of
the image (Starts with 50%). Increasing Manual Gain Control will
increase the RGB average values (Image brighter). Decreasing
Manual Gain Control will decrease the RGB average values
(Image darker).
11. Once the image brightness and color are set, click O OKK and in the
Camera Control panel, click the SSeett TTw ain
wain button to store
settings in the Twain driver. Click YYeess in the confirm dialog box.

Before performing the next steps verify that the G3Utility version is
1.170 or higher. From the pull-down menu go to ‘Help → About
G3Utility’ to identify the G3Utility version.

12. Select SSiizzee SSeettttiinnggss and check that the image size is set to
640x480. Click the O OKK button
13. Click on SSaavvee IIm
maaggee. Enter in the file name field the date and
iteration of the image and click SSaavvee. An example of file name is

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Level 4 – Form Euro 323 – Rev:2
 1-1-2008-02 in which 02 is the second time that a camera image
calculation was performed.
14. This will generate a Camera Image Calculation table. Verify the
RGB Averages are at 180 ± 5, the Max Diffs are less that 65 and
the Deviation is less than 15. „No errors or warnings detected‟
should be displayed in the output view.
15. If the calculated values are out of range, repeat steps 10 to 13.

Figure 1-16: Camera Image Calculations

Because the camera lamp remains on during camera setup in the

G3Utility, set the RGB Averages within the range of 184-188. This will
allow the Camera calibration through the Echo control software to set
the Averages closer to 180.

Refer to: chapter 6 'Alignments and Calibrations – section 1.3.5

Camera Colour Settings' for more details.

16. Adjusting the top chamber camera alignment:

a. Select the Diagnostics menu to open the Move
Robot/Probe dialog box to make adjustments to the
position of the strip holder and strips. On the left side of
this dialog box, there are step size selections. 16 are
equal to one full step. When adjusting the X and Y
positions, use 4 or 16 step sizes.
b. If necessary, adjust the X and Y directions until the four
wells are centred in the image. Refer to Figure 1-18.

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Level 4 – Form Euro 323 – Rev:2
 Figure 1-17: Diagnostic menu: Move Robot/Probe

c. When moving the Z axis position, this is adjusting the

focus of the strips and 100 step sizes are the most useful.
Inspecting the marks inside the strip will aid in visualizing
whether the strips are in focus or not.
d. If necessary, adjust the Z direction until the X‟s in the
bottom of the wells are in focus. Refer to Figure 1-18.
e. Once all adjustments have been made, select the
Configuration menu and then the Strip Transfer 
Autocal option to open the Strip Transfer Board Autocal
Data window:

Figure 1-18: Strip Transfer Board Autocal Data

f. Enter the values of the X, Y and Z coordinates displayed

on the right side of the Move Robot/Probe dialog box into
the Camera X, Camera Y and Camera Z fields of the
Strip Transfer Board Autocal Data table. Click on the

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 „CONFIG_DATA‟ command line in the ICL file and click on
the R
Ruunn C
Coom
mmmaanndd button to execute and save the new
values. Verify that the command return has Status=0 in
the G3 Utility Command Output view.

Refer to: chapter 6 'Alignments and Calibrations – section 1.3.4

Camera Alignment' for more details.

Figure 1-19: Top Chamber Camera Align

17. Adjusting the bottom chamber read:
a. Continuing from the Top chamber adjustment, step all
command lines of the CAMERA ALIGN.ICL file through
the third „TRANSPORT_HOLDER‟ command. The
highlight bar should be located on the last
„TRANSPORT_HOLDER‟ command line. Verify in the
Camera dialog box view that this is located in the bottom
chamber of the Camera module and that the tabs are
visible on the strips.
b. Open the MIRROR ALIGN.ICL file in the G3 Utility and
adjust the mirror rotation so that the tabs and the first full
well are visible on the strips. This is done by un-checking
the Step box and selecting either the first or second
„MOVE_AXIS‟ command from the ICL file. The first
„MOVE_AXIS‟ command will move the image in an
upward direction and the second will move the image in a
downward position. Click on the R Ruunn C
Coommm
maanndd button
and count how many times the button is clicked until only
the tabs and first full well are present in the image.

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 c. Once complete, select the Configuration menu and then
the Strip Transfer  Autocal option to open the Strip
Transfer Board Autocal Data table.
d. Add or subtract the count value from the Mirror Autocal 2
field value of the Strip Transfer Board Autocal Data table.
e. Click on the „CONFIG_DATA‟ command line and click on
the RRuunn C
Coom
mmmaanndd button to save the new value. Verify
that the command return has Status=0 in the G3 Utility
Command Output view

Refer to: chapter 6 'Alignments and Calibrations – section 1.3.3

Mirror Alignment' for more details.

Figure 1-20: Bottom Chamber Mirror Align

18. Re-open the CAMERA ALIGN.ICL file and select the last
„TRANSPORT_HOLDER‟ command. Check the Step box and
press the R Ruunn C
Coom
mmmaanndd button until the strip holder is returned
to the strip tray and the lamp is off.

1.2.5 Probe assembly and fluidics

Probe assembly 1. Run the SAVE SENSOR POSITIONS.ICL file.

alignment 2. Verify robot and probe home positions.
3. Load two strip holders in positions 1 and 4 of pipetting area (Strip
load station).
4. Load a sample rack with 5 empty tubes in lanes 1 and 4.
5. Load a reagent rack with 4 empty bottles in lanes 1 and 4.
6. Run the PROBE AUTOCAL VERIFY.ICL file.
7. Verify that all alignments are correct.
8. If adjustments required refer to: chapter 6 'Alignments and
Calibrations – section 1.2.2 Autocalibration'.

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 9. Run the PROBE ALIGN.ICL file to perform the probe alignment
check. The probe should be centred with the alignment hole at
the top of the rinse station. Adjust the probe if necessary.
10. Remove the strips, sample racks and reagent racks when
complete.
11. Fill the supply bottle with PBS and connect the bottle to the
supply line (tubing and level sensor).
12. Run the LONG SYSTEM PRIME.ICL file.
13. Observe the rinse station and verify that fluid is being evacuated.
14. Observe the wash manifold and verify that fluid is being
dispensed to and aspirated from all wells.
15. Check for leaks at all plumbing connections and inspect tubing
for bubbles.
Peri-pump 16. From the Configuration menu (G3 Utility Options), turn off the
performance gating feature by un-checking the „Instrument Gating‟ option.

Figure 1-21: Instrument Gating off

17. Tare an empty 10ml vial and put it at position 1 of a reagent rack.
18. Insert the reagent rack in lane 1.
19. Run the TEST PERI-PUMP.ICL file
20. Weigh the bottle filled up with PBS and calculate the volume of
liquid.
21. Record the measured value in the IQ form and check that the
volume is within the acceptable range.
22. From the Configuration menu (G3 Utility Options), turn on the
gating feature by checking the „Instrument Gating‟ option.

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 Figure 1-22: Instrument Gating on

Probe accuracy 23. Number four empty strips, weigh them and load them into two
strip holders.
24. Load the strip holders in positions 1 and 2 of ambient incubator
tray 1.
25. Place a bottle filled with 10mL of PBS in position 1 of a reagent
rack.
26. Insert the reagent rack in lane 1.
27. Run the PROBE ACCURACY.ICL file.
28. Weigh each strip and record the volumes in the IQ form.
29. Verify that the measured values are within the acceptable range.

1.2.6 Washer

Check the 1. Verify that the manifold tubes and well sensor pins are not bent.
wash 2. Number two empty strips, weigh them and load them in a holder.
dispense 3. Load the strip holder in position 1 of ambient incubator tray 1.
accuracy 4. Run the WASH DISPENSE ACCURACY.ICL file.
5. Weigh each strip and record the measured value in the IQ form.
Compare with acceptable range.
6. Verify the wash precision by visually inspecting the fill volume of
the strips. Liquid level should be consistent between the 8 wells
of a strip.
Check the 7. Tare 2 strips and load them in a strip holder.
residual 8. Load the strip holder in position 1 of ambient incubator tray 1.
Volume 9. Run the RESIDUAL VOLUME.ICL file.
10. Weigh both strips and record the measured volume in IQ form.
11. Compare with acceptable range.
12. Verify the residual volume by visually inspecting the volume of
the strips. Liquid level should be consistent between the 8 wells
of a strip.
13. If adjustments required (residual volume too low or too high)
please refer to: chapter 6 'Alignments and Calibrations –
section 1.3.5 ‘Washer Residual Volume Adjustment'.

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 1.2.7 Stirrer mechanism

1. Place a reagent vial filled with water and with a stir ball in
position 4 of a reagent rack and insert it in lane 1 of the reagent
bay.
2. Run the TEST STIRRER.ICL file.
3. Observe that the stir ball always stays in contact with the bottom
of the bottle at any speed.
4. If the stir ball looses contact, the clearance between the moving
magnet and the base plate of the reagent bay must be adjusted.
Please refer to: chapter 6 'Alignments and Calibrations –
section 1.3.6 ‘Stirrer Clearance Adjustment'.

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 1.3 Checks with the Application Software

A run of the application (Control) software should be performed to verify

additional functional aspects. Refer to the ‘Galileo Echo operating
manual’ on how to use the control software.

1.3.1 Loading Assay Files

1. Go to folder C:\G3\Assay and open the market specific folder

(i.e. EU, Japan, US).
2. Copy the assay files and paste them directly in the Assay folder.

1.3.2 Instrument initialization

1. Install the top cover on the main instrument from the front: -
engage the bottom left and right casters on the corresponding
guide rails and slide the cover all along the rails. It is not
necessary to restart the instrument after installing the cover.
2. Launch the Galileo Echo control software.
3. Login with appropriate service user name and password.
4. Initialize the instrument and verify that all components of
instrument initialization pass.
5. Re-check for leaking at plumbing connections.
6. Go to Tools-General options-Service and enter the instrument
serial number in the correct field.
7. Open the Camera report and view the Calibration image. Verify
that the RGB Averages are 180 ± 5, the Max Diffs are less than
65, and the Deviation is less than 15.

If Camera calibration fails during initialization, open the ‘Camera’

report from the menu. You will have access to the calibration
details: check if the top and bottom chambers are aligned properly
and check that the RGB values are close to 180. Repeat either the
camera or mirror alignment if necessary or the colour settings
adjustment as described in section 1.2.4.

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 Figure 1-23: Camera report – RGB values

1.3.3 Incubators display

1. Inspect the temperature display of the incubator devices on the

instrument map:

o Incubator 1 sensor 1
o Incubator 1 sensor 2
o Incubator 2
o Incubator 3
o Ambient incubator

1.3.4 Verification of barcode reading

1. Check that the „Strip Codabar prefix and postfix‟ option is

activated for both samples and donors through the Tools →
Barcode options menu.
2. Verify that Immucor reagent barcodes are correctly read in all
positions of the reagent bay.
3. Verify that all required sample barcode types are correctly read
in all positions of the sample bay.
4. Verify that all strip barcodes are correctly read and recognized by
the instrument.

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 1.3.5 Version Verification and Backup

1. Attach a printout of system report file.

2. Compare the current versions with the software master system
report.
3. Insert disk into DVD-ROM drive. Go to My Computer and right
click on the D:\ drive and select Format.
a. In the Label field, enter the instrument serial number,
backup and the date of the backup. I.e. M001 backup 01-
01-2006. Click SSttaarrtt and then click YYeess to start formatting.
b. Copy the ConfigInstrument folder from C:\Program
Files\Biotek\Immucor G3Utility\ to the backup
disk
c. Copy the Assay folder from C:\G3\ to the backup disk.
d. Copy the Basecode folder from C:\ICL Files\ to
the backup disk.
e. Copy the Image.dll and IIS.dll files from C:\G3\ to the
backup disk.
f. Copy all the configuration files (*.INI) from C:\G3\ to the
backup disk.
4. Label the outside of the disk with the same information as
described in step a. and place the disk in the disk sleeve on the
back of the instrument.

1.3.6 Debugging tool verification

1. Verify that the Dr. Watson debugging tool is turned on by clicking

Start from the Taskbar and clicking Run. Type in drwtsn32 and
click O
OKK.
a. Verify the Log File Path is C:\Documents and
Settings\All Users\Application
Data\Microsoft\Dr Watson
b. Verify the Crash Dump is C:\Documents and
Settings\All Users\Application
Data\Microsoft\Dr Watson\user.dmp
c. Verify the Crash Dump type is set to Mini
d. Verify that the following three boxes are checked: Dump
All Thread Contents, Append to Existing Log File, and
Create Crash Dump File.

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 Figure 1-24: Dr Watson for Windows

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2 System de-installation and re-packing

2.1 Preparation

1. Decontaminate the system (if it has been in contact with samples

and/or reagents) with the appropriate maintenance task.
2. Purge the instrument with the corresponding maintenance task.
3. Exit software by selecting „Shut down‟ procedure.
4. Switch off power supply.

2.2 Procedure

1. Remove power supply.

2. Disconnect the serial cable and USB cable from the PC.
3. Repack the computer.
4. Remove the top cover from the main instrument (Will be packed
with Fluidics unit).
5. Remove all the accessories: sample and reagent racks, strip
holders, ambient incubator trays, waste and supply bottles.
Don’t forget to remove the wash prime holder!

6. Remove the probe. Follow countdown steps 6 to 1 as described

in Figure 1-9: Instrument probe installation.
7. Disconnect the data cables from the main instrument and fluids
module.
8. Remove the supply tubing and waste tubing from the main
instrument and fluids module.
9. Pack the accessories as described in Figure 1-7: Accessories
shipping assembly
10. Pack the fluidics unit as described in Figure 1-6: Fluidics unit
shipping assembly
11. Fix the centrifuge module with the 3 hex screws on the
instrument chassis
12. Mount the 5 shipping brackets.
13. Pack the main instrument in respect of Figure 1-1: Main
instrument shipping assembly
Take care – 2 people are required for this step!

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