BIOCHEMISTRY BOARD EXAM QUESTIONS: A___11.

Aminoacyl t-RNA is required for all, EXCEPT:

A. Hydroxyproline C. Cyteine
NAME: B. Methionine D. Lysine

A___ 1. A patient is diagnosed with problems in emptying D___ 12. Which of the following proteins cannot be
the gallbladder. On a blood checkup, one hormone/peptide phosphorylated using Protein Kinase in prokaryotic
that is known to stimulate gallbladder contraction is organisms:
inappropriately low. Which of the following hormones is A. Threonine C. Serine
most likely deficient leading to the observed symptoms? B. Tyrosine D. Asparagine
A. cholecystokinin, CCK
B. gastrin C___ 13. Carboxylation of clotting factors by Vitamin K is
C. GLP-1 required to be biologically active. Which of the following
D. pancreatic polypeptide amino acid is carboxylated?
E. peptide tyrosine tyrosine, PYY A. Histidine C. Glutamate
B. Histamine D. Aspartate
B___ 2. Which of the following familial cancers results from
a defect in the tumor suppressor gene, p53? D___ 14. The property of proteins to absorb ultraviolet rays
A. familial adenomatous polyposis coli (FAP) of light is due to:
B. Li Fraumeni syndrome A. Peptide bond C. Disulfide bond
C. neurofibromatosis type 1 B. Imino group D. Aromatic amino acid
D. retinoblastoma
E. Wilms tumor A___ 15. All biologically active amino acids are:
A. L-forms C. Mostly D-forms
B___ 3. Which of the following is considered an alkylating B. D-forms D. D- and L- forms
agent and has been shown to be effective in cancer
chemotherapy? A___ 16. Flexibility of protein depends on:
A. doxorubicin A. Glycine C. Phenylalanine
B. cyclophosphamide B. Tryptophan D. Histidine
C. methotrexate
D. vinblastine A___ 17. Which amino acid can protonate and deprotonate
E. Etoposide at neutral pH:
A. Histidine C. Glycine
C___ 4. All of the following are essential amino acids, B. Leucine D. Arginine
EXCEPT:
A. Methionine C. Alanine C___ 18. Which of the following amino acid is purely
B. Lysine D. Leucine Glucogenic:
A. Valine C. Alanine
D___ 5. Nonpolar amino acids are the following, EXCEPT: B. Lysine D. Glycine
A. Alanine C. Isoleucine
B. Tryptophan D. Lysine A___ 19. Transfer of an amino group from an amino acid to
an alpha keto acid is done by:
A___ 6. The following are basic amino acids, EXCEPT: A. Transaminases C. Tranketolases
A. Leucine C. Lysine B. Aminases D. Deaminases
B. Arginine D. Histidine
A___ 20. The amino acid which serves as a carrier of
B___ 7. Guanidinium group is associated with: ammonia from skeletal muscle to liver is:
A. Tyrosine C. Tryptophan A. Alanine C. Arginine
B. Arginine D. Histidine B. Methionine D. Glutamine

A___ 8. Sulfur containing amino acid is: C___ 21. Amino acid absorption is by:
A. Cysteine C. Arginine A. Facilitated transport C. Active transport
B. Leucine D. Threonine B. Passive transport D. Pinocytosis

A___ 9. Which of the following side chains is least polar: D___ 22. Urea cycle enzymes are:
A. Methyl C. Amino A. Glutaminase C. Glutamate dehydrogenase
B. Carboxyl D. Phosphate B. Asparginase D. Ornithine transcarbamoylase

C___ 10. Amide group containing amino acid is: C___ 23. Urea cycle occurs in:
A. Glutamate C. Glutamine A. Cytoplasm C. Both cytoplasm & mitochondria
B. Glutamic acid D. Aspartate B. Mitochondria D. Endoplasmic reticulum
B___ 24. The following enzymes are NOT involved in Urea A___ 36. Histidine load test is used for:
cycle, EXCEPT: A. Folate deficiency C. Histamine deficiency
A. Glutamate dehydrogenase B. Histidine deficiency C. Tyrosine deficiency
B. Isocitrate dehydrogenase
C. Fumarase C___ 37. In glutathione which amino acid is the reducing
D. Argininosuccinate synthetase agent:
A. Glutamic acid C. Cysteine
D___ 25. Glutamate dehydrogenase in mitochondria is B. Glycine D. Alanine
activated by:
A. ATP C. NADH A___ 38. Branched chain ketoacid decarboxylation is
B. GTP D. ADP defective in:
A. Maple syrup urine disease C. Alkaptonuria
D___ 26. Enzyme involved in nonoxidative deamination is: B. Hartnup disease D. GMI Gangliosidosis
A. L-amino acid Oxidase C. Glutaminase
B. Glutamate dehydrogenase D. Amino acid dehydrase C___ 39. Histidine is converted to Histamine by which
reaction:
C___ 27. Dopamine is synthesized from: A. Carboxylation C. Decarboxylation
A. Tryptophan C. Tyrosine B. Oxidation D. Amination
B. Threonine D. Lysine
C___ 40. Which of the following amino acid is excreted in
B___ 28. A 40yo woman presents with palmoplantar urine in maple syrup urine disease:
pigmentation. Xray spine shows calcification of IV disk. On A. Tryptophan C. Leucine
adding Benedicts reagent to urine, it gives greenish brown B. Phenylalanine D. Arginine
precipitate and blue-black supernatant fluid. What is the
diagnosis: D___ 41. Oxaloacetate is formed from:
A. Phenylketonuria C. Tyrosinemia type 2 A. Proline C. Glutamate and Glutamine
B. Alkaptonuria D. Arigininosuccinic aciduria B. Histidine & Arginine D. Aspartate & Asparagine

B___ 29. Mousy body odor is due to: D___ 42. Smell of sweaty feet is seen in:
A. Phenylalanine C. Phenylbutazone A. MSUD C. Homocystinuria
B. Phenylacetate D. Phenylacetylglutamine B. Phenylketonuria D. Glutaric acidemia

A___ 30. Enzyme deficiency in albinism is: C___ 43. Succinyl CoA is formed by:
A. Tyrosinase C. Phenylalanine hydroxylase A. Histidine C. Valine
B. Tyrosine hydroxylase D. Homogentisate oxidase B. Leucine D. Lysine

B___ 31. Correct combination of Urine odor in various A___ 44. The following are storage proteins, EXCEPT:
metabolic disorder: A. Myoglobin C. Ferritin
A. Alkaptonuria : Rotten egg B. Ovalbumin D. Glutelin
B. Tyrosinemia : Rotten cabbage
C. Hawkinsinuria : Potato smell A___ 45. Biuret test is used for detection of:
D. Maple syrup disease : Rotten tomato A. Protein C. Steroid
B. Cholesterol D. Sugar
A___ 32. Glycine cleavage system in liver mitochondria is
associated with which enzyme: A___ 46. Polypeptide formation in amino acid is by:
A. Glycine dehydrogenase C. Glycine Decarboxylase A. Primary structure C. Tertiary structure
B. Glycine transaminase D. Glycine dehydratase B. Secondary structure D. Quaternary structure

A___ 33. Conversion of glycine to serine requires: A___ 47. Denaturation is resisted by which of the following
A. Folic acid C. Fe 2+ bond:
B. Thiamine D. Vit C A. Peptide bond C. Disulfide bond
B. Hydrogen bond D. Electrostatic bond
A___ 34. Cysteine is abundantly found in:
A. Keratin C. Creatine B___ 48. What type of protein is Casein:
B. Chondroitin sulfate D. Spermine A. Lipoprotein C. Glycoprotein
B. Phosphoprotein D. Flavoprotein
A___ 35. N-acetyl-cysteine replenishes:
A. Glutathione C. Glutamate B___ 49. Which is NOT a transport protein:
B. Glycine D. GABA A. Transferrin C. Ceruloplasmin
B. Collage D. Hemoglobin
A___ 50. Proteins are sorted by: is used for liver function assessment:
A. Golgi bodies C. Ribosomes A. Galactose tolerance test C. Glucose tolerance test
B. Mitochondria D. Nuclear membrane B. Sucrose tolerance test D. Lactose tolerance test

B___ 51. Not a finction of endoplasmic reticulum: D___ 65. Which deposition results in cataract:
A. Protein synthesis C. Protein sorting A. Glucose C. Sugar amines
B. Muscle contraction D. Glycoproteins B. Galactose D. Sugar alcohols

D___ 52. Which of the following groups of proteins assist in C___ 66. Cellulose is:
the folding of other proteins: A. Complex lipoprotein C. Nonstarch polysaccharide
A. Proteases C. Templates B. Starch polysaccharide D. Complex glycoprotein
B. Proteosomes D. Chaperones
A___ 67. Glycosaminoglycans present in cornea:
B___ 53. Immunoglobulins are: A. Keratan sulfate C. Chondroitin sulfate
A. Proteins C. Proteoglycan B. Hyaluronic acid D. Dermatan sulfate
B. Glycoproteins D. Glycoside
A___ 68. Mucopolysaccharidoses, which are a lysosomal
B___ 54. Suicidal Enzyme is: storage disease, occur due to abnormality in:
A. Lipoxygenase C. Thromboxane synthase A. Hydrolase enzyme C. Lipase enzyme
B. Cyclooxygenase D. 5’ Nucleotidase B. Dehydrogenase enzyme D. Phosphatase

A___ 55. Which of the following is a Lyase: C___ 69. After overnight fasting, levels of glucose
A. Aldolase B C. Fatty Acyl CoA dehydrogensae transporters are reduced in:
B. Acetyl CoA synthetase D. Acetyl CoA carboxylase A. Brain cells C. Adipocyte
B. RBCs D. Hepatocyte
B___ 56. All of the following enzymes are involved in
oxidation-reduction, EXCEPT: D___ 70. Glucose transporter in myocyte stimulated by
A. Dehydrogenases C. Oxygenases insulin is:
B. Hydrolases D. Peroxidases A. GLUT-1 C. GLUT-3
B. GLUT-2 D. GLUT-4
C___ 57. Coenzyme in decarboxylation reaction:
A. Niacin C. Pyridoxine A___ 71. Glucose transporter present in the RBC:
B. Biotin D. Riboflavin A. GLUT-1 C. GLUT-3
B. GLUT-2 D. GLUT-4
C___ 58. The following affect enzyme activity, EXCEPT:
A. Methylation C. Induction A___ 72. Cancer cells derive nutrition from:
B. Acetylation D. Phosphorylation A. Glycolysis
B. Oxidative phosphorylation
A___ 59. Chymotrypsinogen is a: C. Increase in mitochondria
A. Zymogen C. Transaminase D. From a fast food joint
B. CarboxypeptidaseD. Exopeptidase
B___73. The number of ATPs produced by Rapaport
D___ 60. Peroxidase enzyme is used in estimating: leubering Cycle in RBC from Glucose:
A. Hemoglobin C. Creatinine A. 1 C. 3
B. Ammonia D. Glucose B. 2 D. 4

A___ 61. Red color are produced by the following in B___ 74. Which of the following does NOT contribute to
Benedict’s test, EXCEPT: glucose by gluconeogenesis:
A. Inositol C. Lactose A. Lactate C. Pyruvate
B. Fructose D. Maltose B. Acetyl CoA D. Oxaloacetate

C___ 62. Which of the following is NOT an aldose: C___ 75, A child with low blood glucose is unable to do
A. Glucose C. Fructose glycogenolysis or gluconeogenesis. Which of the following
B. Mannose D. Galactose enzyme is missing in the child:
A. Fructokinase C. Glucose-6-Phosphatase
A___ 63. All are reducing sugars, EXCEPT: B. Glucokinase D. Transketolase
A. Sucrose C. Glucose
B. Lactose D. Fructose
B___ 76. During prolonged fasting, rate of gluconeogenesis
A___ 64. Which of the following carbohydrate metabolisms is determined by:
A. Essential fatty acid in liver
B. Alanine in liver C___ 90. Which coenzyme act as reducing agent in anabolic
C. Decreased cGMP reaction:
D. ADP in liver A. FADH2 C. FMNH2
B. NADPH D. NADH
C___ 77. Glucose can be synthesized from all, EXCEPT:
A. Amino acids C. Acetoacetate C___ 91. All are derived from cholesterol EXCEPT:
B. Glycerol D. Lactic acid A. Vit. D C. Bile pigment
B. Bile salt D. Steroid
A___ 78. Acetyl CoA can be converted into all of the
following, EXCEPT: A___ 92. Triglycerides are maximum in:
A. Glucose C. Cholesterol A. Chylomicrons C. LDL
B. Fatty acids D. Ketone bodies B. VLDL D. HDL

A___ 79. Glycogen is released from the muscle because of D___ 93. Termination process of protein synthesis is
increased cAMP due to: performed by all, EXCEPT:
A. Epinephrine C. Glucagon A. UAA codon C. Peptidyltransferase
B. Thyroxine D. Growth hormone B. Stop codon D. AUG codon

B___ 80. Cofactor for Glycogen Phosphorylase: A___ 94. Shine-Dalgarno sequence in bacterial mRNA is
A. Thiamine Pyrophosphate C. Citrate near:
B. Pyridoxal phosphate D. FAD A. AUG codon C. UAG codon
B. UAA codon D. UGA codon
C___ 81. Pompe’s disease is due to deficiency of:
A. Debranching enzyme C. Acid maltase D___ 95. Vitamin K is required for:
B. Muscle phosphorylase D. Branching enzyme A. Hydroxylation C. Transamination
B. Chelation D. Carboxylation
D___ 82. Reduced NADPH is produced from which pathway:
A. Krebs cycle C. Uronic acid pathway C___ 96. Vitamin E deficiency causes all, EXCEPT:
B. Anerobic glycolysis D. HMP pathway A. Ataxia C. Ophthalmoplegia
B. Areflexia D. Neuropathy
C___ 83. NADPH is produced by:
A. Glycolysis C. HMP shunt A___ 97. Vitamin B12 is NOT required for:
B. Citric acid cycle D. Glycogenesis A. Glycogen phosphorylase
B. Methionine Synthase
A___ 84. The following are products of uronic acid pathway C. Methyl malonyl CoA Mutase
in human beings, EXCEPT: D. Leucine amino mutase
A. Vit C C. Pentoses
B. Glucuronic acid D. NADH B___ 98. Most powerful chain breaking antioxidant:
A. Glutathione peroxidase C. Superoxide dismutase
C___ 85. NADPH is required for: B. Alpha tocopherol D. Vitamin C
A. Gluconeogenesis C. Fatty acid synthesis
B. Glycolysis D. Glycogenolysis D___ 99. Megaloblastic anemia is seen in:
A. Ornithine transcarbamoylase defect
A___ 86. The first step in fatty acid synthesis involves: B. MSUD
A. Acetyl CoA carboxylase C. Acetyl dehydrogenase C. Citrullinemia
B. B-Hydroxyl CoA dehydrogenase D. Pyruvate kinase D. Orotic aciduria

C___ 87. In fatty acid synthesis CO2 loss occurs in which step: B___ 100. Which of the vitamin deficiency leads to lactic
A. Hydration C. Condensation reaction acidosis:
B. Dehydration D. Reduction A. Riboflavin C. Niacin
B. Thiamine D. Pantothenic acid
D___ 88. Rothera’s test is used for detection of:
A. Proteins C. Fatty acid
B. Glucose D. Ketones

A___ 89. Which organ does NOT utilize ketone bodies:

A. Liver C. Skeletal muscle
B. Brain D. Cardiac muscle