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NICFS
DISCLAIMER
This publication is meant for authorized Criminal Justice Agencies. LNJN NICFS shall
not be liable for any direct, consequential or incidental damages arising out of the use
of the book. Reference herein to any specific product (commercial or non-commercial),
processes or services by brand or trade name, trademark, manufacturer, or otherwise
does not necessarily constitute or imply its endorsement, recommendation or favour by
LNJN NICFS (MHA). The information and statements contained in this document
shall not be used for the purpose of advertising or to imply the endorsement or
recommendation of LNJN NICFS (MHA).
Published by
LNJN National Institute of Criminology and Forensic Science
(Ministry of Home Affairs)
Sector-3, Outer Ring Road, Delhi –110085.
Executive Editor
Rina Mitra, IPS
Co-Executive Editor
Sandeep Mittal, IPS
Credits
Copy Editor: Sadiksha Waiba
Jacket Design: Abriti Waiba
Cover photo: Gandhi Smriti and Darshan Samiti, New Delhi
Printed at
Bengal Offset Works,335, Khajoor Road, Karol Bagh, New Delhi-110005.
NICFS
FOREWORD
(ANIL SINHA)
Director
Central Bureau of Investigation
New Delhi.
NICFS
NICFS
PREFACE
PREFACE
The scope and importance of forensic evidence has been increasing over the years. On one hand,
with the expansion of technology, new types of crime such as internet based crime, computer aided
crime, etc., have emerged as a major challenge to the law enforcement agencies. Consequently,
forensic science has also expanded into new areas like computer forensics. On the other hand, there
is increasing demand and reliance on forensic evidence by courts and opinion makers. In the recent
past, courts have relied on forensic evidence over oral evidence for conviction. Therefore,
investigating agencies are expected to not only collect newer types of evidences but also to strive to
get relevant and convincing forensic evidence to satisfy the courts as well as other stakeholders like
the press, civil society and political leadership.
Each type of forensic evidence requires relevant method for collection, handling, packing and
forwarding the physical evidence for forensic examination. However, crucial a forensic evidence
may be, any anomaly in the way it was collected and handled could lead to destruction of its
evidentiary value. Forensic evidence can be severely damaged due to shortcoming of any of
the aspects. While Investigating officers (I.Os) try to collect physical evidence for forensic
examination, often they are not aware of the exact procedure and also relevant queries.
Investigating Officers need to know the correct procedure for each type of forensic evidence
at the time of collection and also queries that could be made for the expert’s opinion.
Though there is a tendency to send specialized officers for collection of forensic evidence, it is
neither necessary nor practical for the team to reach each and every place. Therefore, the I.O. himself/
herself should be capable of handling physical evidence.
However, looking at the vast range of physical evidences that need forensic examination, it is not
possible for an I.O. to remember the correct procedure in detail. Therefore, it was felt that a handbook
on Standard Operating Procedures (SOPs) should be available to the I.O. for their ready reference.
This handbook tries to fulfill the long felt need of the investigating agencies. It will be useful not
only for I.Os. but also judicial officers and prosecutors. This is a maiden attempt by NICFS intended
to empower different agencies of the criminal justice system and bridge the gap between technical
and non-technical officials in the criminal justice system.
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A Forensic Guide for Crime Investigators NICFS
PREFACE
While drafting the chapters of this voluminous work, care has been taken and chapters have not
only been reviewed by the internal faculty but also vetted by external experts on the relevant subject.
Effort has also been made to make the book user friendly and thus empower the I.Os. with basic
understanding of the type of crime and investigation they would handle. Forensic Science is also a
growing discipline.Vital suggestions received from the users, the state police or investigation agencies
under the central government, trainers as well as heads of training institutions may need to be
incorporated in the next edition.
This SOP may not be all inclusive. However, due to technology growing at rapid pace, the suggestions
in the SOP are best examined in the context of current technology and practices prevalent in the
country. Besides, each case is unique; therefore it may require action other than those described in
the related chapter. Therefore, users may have to refer to the other chapters also for the appropriate
forensic tool that suits them.
This handbook also provides model queries that the I.O. needs to ask the forensic experts and
medico legal experts. This book on Standard Operating Procedure (SOP) is for ready reference only
and doesn’t claim the absolute necessity or correctness of the prescribed procedure.
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NICFS A Forensic Guide for Crime Investigators
ACKNOWLEDGEMENTS
ACKNOWLEDGEMENTS
A Forensic Guide for Crime Investigators: Standard Operating Procedures has been written to
bridge the gap in application of Forensic Science on crime scene management and to guide
the Investigating Officers. We wish to thank the chapter authors and subject experts who
helped us in achieving our endeavour.
We thank all the Director General of Police of State Police especially Tamil Nadu, Arunachal
Pradesh, Haryana, Director General, National Investigation Agency and Director, State Vigilance
Bureau, Haryana for providing their support in the creation of this forensic guide.
We wish to acknowledge and communicate our gratitude to Shri M.L.Sharma, IPS, Former
Special Director, Central Bureau of Investigation, who volunteered to edit the contents of this
voluminous work and Shri R.R.Verma, IPS, then Director General, National Crime Records
Bureau, New Delhi, who extended all his support to prepare chapters on finger prints and
portrait building.
Our thanks to the Director General, Narcotics Control Bureau, New Delhi and Director,
Wildlife Crime Control Bureau, New Delhi, for their unqualified support.
We thank our former Director Smt. Rina Mitra, IPS and Shri Sandeep Mittal, IPS, Deputy
Inspector General of Police (Administration), NICFS, Delhi, for leading the team of faculties
of NICFS and coordinating with other organizations for bringing in valuable inputs to this
forensic guide.
We would also like to acknowledge the sincerity, hard work and innovative ideas put in by
Ms Sadiksha Waiba for copy editing and enhancing the visual appeal of the voluminous
work.
Lastly,we are particularly beholden to the staff of LNJN NICFS who actively participated and
contributed in the publication of this volume.
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A Forensic Guide for Crime Investigators NICFS
SUBJECT AUTHORS
Subject Authors
Lead Author
Dr A.C. Rajvanshi, Senior Faculty (Chemistry), NICFS, Delhi
Contributing Authors (in alphabetical order)
Dr A. K. Bapuly,Director, FSL, Odisha
Shri N. B. Bardhan, Principal Scientific Officer, CFSL/CBI, Delhi
Dr V. N. Sehgal, Retd. Director, CFSL/CBI, Delhi
Dr Tanya Chauhan, Assistant Professor (Biology), NICFS, Delhi
Chapter 01-General Principles of Scene Investigation General
Chapter 02-Principles of Evidence Packaging
Lead Author
Dr K. P. S. Kushwaha, Senior Faculty (Biology), NICFS, Delhi
Contributing Authors (in alphabetical order)
Dr A. K. Bapuly, Director, FSL, Odisha
Dr M. K. Goel, Former Director, FSL, Haryana
Dr S. K. Verma, Professor of Forensic Medicine & Toxicology, University College of
Medical Science and GTB Hospital , Delhi
Dr V. Dhankar, Specialist & Head, Department of Forensic Medicine, Dr. BSA
Hospital, Rohini, Delhi
Chapter 03-Forensic Biology
Chapter 04-DNA Fingerprinting
Chapter 05-Bloodstain Pattern Analysis
Lead Author
Shri P. Sharma, Senior Scientific Assistant (Chemistry), NICFS, Delhi
Contributing Author (in alphabetical order)
Shri L. K. Katyal, Former Assistant Director, FSL, Haryana
Chapter 06-Poisoning cases
Chapter 07-Hooch cases
Chapter 08-Narcotic Drugs and Psychotropic Substances
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SUBJECT AUTHORS
Lead Author
Shri J. R. Sethi, Assistant Professor (Chemistry), NICFS, Delhi
Contributing Authors (in alphabetical order)
Shri N. B. Bardhan, Principal Scientific Officer, CFSL/CBI, Delhi
Shri Ram AvtarYadav, Deputy Superintendent of Police, Anti Corruption, CBI
Dr V. N. Sehgal, Former Director, CFSL/CBI, Delhi
Chapter 09-Explosives
Chapter 10-Fire and Arson
Chapter 11-Petroleum Adulterations
Chapter 12-Trap Cases
Chapter 13-Acid Attack
Lead Authors
Dr J. K. Modi, Senior Faculty (Ballistics), NICFS, Delhi
Shri C. Nigam, Senior Scientific Assistant (Ballistics), NICFS, Delhi
Contributing Authors (in alphabetical order)
Dr A. K. Bapuly, Director, FSL, Odisha
Shri K. C. Varshney, Deputy Director, FSL, Delhi
Chapter 14-Firearms
Lead Author
Shri J. K. Semwal, Senior Faculty (Documents), NICFS, Delhi
Contributing Authors (in alphabetical order)
Dr A. K. Bapuly, Director, FSL, Odisha
Shri A. K. Gupta, Former Director, FSL, Delhi
Shri Sanjeev Kumar, Senior Scientific Assistant (Documents), NICFS, Delhi
Chapter 15-Forged Documents
Chapter 16-Security Documents
Chapter 17-Torn Documents
Chapter 18-Charred Documents
Lead Author
Shri Anil Chawla, Director In-charge, Central Finger Print Bureau, NCRB, Delhi
Chapter 19-Fingerprints
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A Forensic Guide for Crime Investigators NICFS
SUBJECT AUTHORS
Lead Authors
Shri Sandeep Mittal, Deputy Inspector General of Police (Administration), NICFS,
Delhi
Shri Sanjeev Kumar, Senior Scientific Assistant (Documents), NICFS, Delhi
Contributing Authors (in alphabetical order)
Shri A. K. Dohare, Additional Director General of Police, Bhopal
Shri G. Deepak Raj Rao, Assistant Professor (Computer Forensics), NICFS, Delhi
Shri Om Veer Singh, Additional Director, CERT-IN, Delhi
Chapter 20-Digital Evidence
Lead Author
Dr S. R. Singh, Senior Faculty (Physics), NICFS, Delhi
Contributing Authors (in alphabetical order)
Dr A. C. Rajvanshi, Senior Faculty (Chemistry), NICFS, Delhi
Dr C. P. Singh, Assistant Director (Physics), FSL, Delhi
Chapter 21-Image Analysis – CCTV Footage
Lead Author
Dr S. R. Singh, Senior Faculty (Physics), NICFS, Delhi
Contributing Author (in alphabetical order)
Dr C. P. Singh, Assistant Director (Physics), FSL, Delhi
Chapter 22-Road Accident
Chapter 23-Theft and Burglary
Chapter 24-Speaker Identification
Lead Author
Ms. Surekha Soni, National Crime Record Bureau, New Delhi
Chapter 25-Portrait Building System
Lead Author
Dr A. K. Bapuly, Professional Director, FSL, Bhubaneswar
Chapter 26-Polygraph Test
Lead Author
Smt. Rina Mitra, Director, LNJN NICFS, Delhi
Chapter 27-Wildlife
Chapter 28-Digital Signature of Wildlife Products in International Trade
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CONTENTS
PREFACE i-ii
ACKNOWLEDGEMENTS iii
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2.6.5 Saliva 25
2.6.6 Dust or Soil 26
2.6.7 Arson Cases and Cases of Burning 26
2.6.8 Tool Marks 27
2.6.9 Exhibits for Ballistics Examination 27
2.6.10 Glass 29
2.6.11 Paint 29
2.6.12 Questioned Documents 30
2.6.13 Latent Fingerprints 30
2.6.14 Drug Samples 31
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6. POISONING 112-159
6.1 Sections of Law 112
6.2 List of Equipments Required 114
6.3 Constituting the Team for Handling the Crime Scene 115
6.4 Crime Scene Investigation 116
6.4.1 Protection of Crime Scene 116
6.4.2 Photography of the Crime Scene 117
6.4.3 Sketching of Crime Scene 117
6.4.4 Crime Scene Search 117
6.5 Classification of Poisons 117
6.6 Information to be Obtained while Investigating a Case of Poisoning 119
6.7 Factors Affecting the Effect of Poisons 120
6.8 Examination of the Body of the Victim 120
6.9 Forwarding of Evidences to FSL 126
6.10 Guidelines for Preservation of Exhibits 126
6.11 Checklist on Investigation of Death due to Poisoning 128
6.12 Do’s and Don’ts for the Investigating Officers 128
6.13 Alcohol 129
6.13.1 Fatal Dose 129
6.13.2 Fatal Period 130
6.13.3 Evidence in Cases of Alcohol Intoxication 130
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23.3.2 Materials which may get transferred to the criminal during 543
commission of crime
23.3.3 From Suspect and his Environment 543
23.4 Collection and Preservation of Evidence Material 543
23.4.1 Fingerprints 543
23.4.2 Glass Fragments 544
23.4.3 Tools and Tool Marks Evidences 544
23.4.4 Foot/Shoeprints/Tyre Impression 543
23.4.5 CCTV Recording 545
23.5 Forwarding the Exhibits for Forensic Examination 546
23.6 Queries to be Raised 546
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