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  • Perhitungan Konsentrasi Ekstrak

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    RENATA VANIA
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    The document describes calculations to determine the concentration of an extract in milligrams per milliliter and procedures to make solutions of varying concentrations from 2000 ppm down to 15.625 ppm. Mortality rates of a test population were observed after exposure to each concentration level. A concentration of 1000 ppm resulted in a 20% mortality rate, while concentrations of 500 ppm and below showed 0% mortality across all trials.

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    Vial

    Original Title

    Perhitungan Vial

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    The document describes calculations to determine the concentration of an extract in milligrams per milliliter and procedures to make solutions of varying concentrations from 2000 ppm down to 15.625 ppm. Mortality rates of a test population were observed after exposure to each concentration level. A concentration of 1000 ppm resulted in a 20% mortality rate, while concentrations of 500 ppm and below showed 0% mortality across all trials.

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    © All Rights Reserved
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    18 views4 pages

    Perhitungan Konsentrasi Ekstrak

    Uploaded by

    RENATA VANIA
    The document describes calculations to determine the concentration of an extract in milligrams per milliliter and procedures to make solutions of varying concentrations from 2000 ppm down to 15.625 ppm. Mortality rates of a test population were observed after exposure to each concentration level. A concentration of 1000 ppm resulted in a 20% mortality rate, while concentrations of 500 ppm and below showed 0% mortality across all trials.

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    © All Rights Reserved
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    of 4

    Perhitungan

    Konsentrasi Ekstrak
    Bobot cawan kosong = 59,4 gram
    Bobot cawan + ekstrak = 59,6 gram
    Bobot ekstrak = 0,2 gram
    0,2 gram
    Konsentrasi ekstrak =
    10 mL
    = 20000 ppm

    Variasi Konsentrasi
     Stok 2000 ppm
    V1.N1 = V2.N2
    V1. 20000 = 25 . 2000
    = 2,5 mL

     1000 ppm
    V1.N1 = V2.N2
    N1 . 2000 = 10 . 1000
    = 5 mL
     500 ppm
    V1.N1 = V2.N2
    N1 . 2000 = 10 . 500
    = 2,5 mL
     250 ppm
    V1.N1 = V2.N2
    N1 . 2000 = 10 . 250
    = 1,25 mL
     125 ppm
    V1.N1 = V2.N2
    N1 . 2000 = 10 . 125
    = 0,625 mL
     62,5 ppm
    V1.N1 = V2.N2
    N1 . 2000 = 10 . 62,5
    = 0,3125 mL
     31,25 ppm
    V1.N1 = V2.N2
    N1 . 2000 = 10 . 31,25
    = 0,15625 mL
     15.625 ppm
    V1.N1 = V2.N2
    N1 . 2000 = 10 . 15.625
    = 0.0781 mL

    Vial
    Konsentrasi Akhir Sampel
    Konsentrasi Volume Volume Air Laut Konsentrasi akhir
    awal (ppm) konsentrasi awal + Ragi (mL) (ppm)
    2000 5 5 1000
    1000 5 5 500
    500 5 5 250
    250 5 5 125
    125 5 5 62.5
    62.5 5 5 31.25
    31.25 5 5 15.625

    Persen Kematian
    Konsentrasi Log Kematian/ Rata-rata
    Kematian (%)
    (µg/mL) Konsentrasi Populasi kematian (%)
    1000 3 2/10 20
    20
    2/10 20
    500 2.699 0/10 0
    0
    0/9 0
    250 2.398 0/10 0
    0
    0/10 0
    125 2.097 0/9 0 0
    0/10 0
    62.5 1.796 0/10 0
    0
    0/10 0
    31.25 1.495 0/10 0
    0
    0/10 0
    15.625 1.194 0/8 0
    0
    0/10 0

    %Kematian
    1000 ppm : 2/10 x 100% = 20%
    2/10 x 100% = 20%
    Rata – rata : 20%

    500 ppm : 0/10 x 100% = 0%


    0/9 x 100% = 0%
    Rata-rata : 0%

    250 ppm : 0/10 x 100% = 0%


    0/10 x 100% = 0%
    Rata-rata : 0%

    125 ppm : 0/9 x 100% = 0%


    0/10 x 100% = 0%
    Rata-rata : 0%

    62.5 ppm : 0/10 x 100% = 0%


    0/10 x 100% = 0%
    Rata-rata : 0%

    31.25 ppm : 0/10 x 100% = 0%


    0/10 x 100% = 0%
    Rata-rata : 0%
    15.625 ppm : 0/8 x 100% = 0%
    0/10 x 100% = 0%
    Rata-rata : 0%

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