ซีต้าเทคโนโลยี่ แก้ปัญหาสารพิษเชื้อรา

ซีต้าเทคโนโลยี่ แก้ปัญหาสารพิษเชื้อรา
 ความท้ าทาย
Mycotoxin
 Key Raw material : Mycotoxin contamination
Aflatoxin Zearalenone Fumonisin T2 Toxin
Soy Bean Meal ++ ++ ++ ++
Corn ++++ ++++ ++++ ++
Baked Beans + ++ ++ +
Fishmeal ++ + + +
Broken Rice + + + +
Casava ++ + + +
Rice Bran ++ +++ ++ ++
DDGS ++ ++ +++ +++

ที่มา : คณะสัตวแพทยศาสตร์ มหาวิทยาลัยเกษตรศาสตร์ วิทยาเขตกําแพงแสน

Mycotoxin :
Challenge
 Production of mycotoxins

Harvest

Mycotoxins
Fusarium
Claviceps

Aspergillus
Penecillium

Time
Field Storage
7
7
Mycotoxin Binder
 Mycotoxin Management 真菌毒素管理方案

DO NOT Feed Contaminated raw material

DILUTION 稀释
AMMONIZATION 氨化处理
MYCOTOXIN BINDER
霉菌毒素吸附剂
BIOTRANSFORMATION
生物转化
SUPPORTIVE TREATMENT
补充治疗
 Available Ingredient to Control Mycotoxin

Yeast Cellwall Clay Activated Charcoal Synthetic Enzyme Herb

Quick response ** **** **** **** **
Polar Mycotoxin *** **** **** ****

Nonpolar Mycotoxin ** *** **** ****

Zearalenone ** *** *** ***
Fumonisin * ** **
Ochratoxin ** *
pH 4-7 3-7 4-6 4-6 6-7
Supportive **** *
Immunity *** *
Palatability *** **
Liver Function **
Combine Nutrient *** ****
Easy denature *** **

没有单一的解决方案 !! 10
Effects of mycotoxins on animals
Poultry

Aflatoxin T-2 Toxin

• Growth reduction
• Reduced feed efficiency
• Immunosupression
• Anemia
• Reduced antibiotic
effectiveness
• Anorexia
• Liver damage
• Reduced egg production
• Increased mortality
• Reduced skin pigmentation
• Lethargy
• Reduced ability to metabolize
fat, starch, and protein
• Increased blood clotting time
Zearalenone
• Increased mortality
• Increased susceptibility to
disease
• Increased blood clotting time
• Digestive disorders
• Increased mortality
• Bloody diarrhea
• Reduced egg production
• Intestinal hemorrhages
• Increased susceptibility to
disease
Deoxynivalenol
• Reduced reproductive
performance
Ochratoxin • Increased mortality
• Nephrotoxicity • Reduced feed efficiency
• Reduced feed consumption • Diarrhea
• Pale beaks • Reduced growth
• Weakness and lameness • Reduced egg production
• Ataxia • Neural disturbances
• Tremors • Increased susceptibility to
• Diarrhea disease
• Death • Increased blood clotting time

Source: CAST, Dairlyland labs

Effects of mycotoxins on animals
Swine

Aflatoxin Deoxynivalenol Ergot alkaloids

• Liver damage • Reduced feed consumption / • Reduced growth
• Hydrolyzation into M1 and feed refusal • Vertigo, staggers,
is “eliminated” in milk ( • Vomiting convulsions, temporary
piglets) posterior paralysis
• Immunosupression • Decreased peripheral blood
• Acute signs: anorexia, supply
depression, ataxia, epitasis • Reduced reproductive
• Chronic signs: reduced feed efficiency of sows
efficiency, reduced milk • Eventual death
production, icterus,
decreased appetite

Zearalenone
• Hyperestrogenism
• Vulvovaginitis
T-2 Toxin • Vaginal or rectal prolapses in
• Immunosupression pre-pubertal gilts
• Frequent defecation Ochratoxin
• Reduced feed efficiency • Enlarged uterus, swollen or
• Vomiting twisted uterus, shrunken
• Weight loss • Growth reduction
• Liver and kidney damage ovaries
• Feed refusal • In boars, testes atrophy,
• Increased mortality
enlarged mammary glands,
decreased fertility

Source: CAST, University of Minnesota

Effects of mycotoxins on animals
Ruminants

Aflatoxin T-2 Toxin

• Reduced feed efficiency • Feed refusal
• Growth reduction • Reduced production
• Hydrolyzation into M1 and is • Gastroenteritis and intestinal
“eliminated” in milk ( hemorrhages
humans) • Reduced immunological
• Tachycardia response in calves
• Tachypnoea • Abortions
• Liver damage • Death
• Death

Zearalenone
• Hyperestrogenism
Deoxynivalenol • Vulvovaginitis
• Reduced feed consumption / • Vaginal secretion
feed refusal • Abortions
• Vomiting • Reduced feed consumption
• Reduced milk production • Reduced milk production
• Increased count of somatic • Reduced reproductive
cells in milk Esporidesmine
• Eczema facial efficiency
• Reduced reproductive
efficiency

Source: NCSU, CAST

 Aflatoxins

 fatty liver
 kidney disorders
 leg and bone problems
 pigmentation problems (carcasses, egg yolk)
 reduced hatchability of eggs,
 smaller eggs, eggshell quality affected
 coccidiosis
 vaccine failure
 reduced immunity, lower resistance to diseases,
bacteria, viruses…
 reduced performances (weight gain, FCR, mortality)
 Ochratoxin

 increased mortality
 reduced growth and FCR
 feed refusal
 diarrhoea, tremors and other neural malfunctions (high
doses).
 reduced egg production
 weaker eggshell
 eggs with blood and meat spots.
 Trichothecenes (ex T-2)

 lesions at the edges of the beaks

 lesions in the intestine.
 decreased feed intake, weight loss
 anomalous feathering in chicks
 drop in egg production
 eggs with weaker shells
 Maximum levels of mycotoxin
recommended in poultry feeds
(Dr. Jowaman Khajarern – Thailand)
Mycotoxin (ppb)

Animal AF ZEN T2 OC FMN DON

Duckling 10-40 <100 100-200 <100 <300 <300

Starter Broiler 20-30 <100 100-200 100 <300 <300
Grower Broiler 60-80 <200 200-300 <200 <500 <300

Layer/Breeder 40-50 <100 100-200 <100 <300 <300

AF = Aflatoxin ZEN = Zearalenone T2 = T-2 toxin

OC = Ochratoxin FMN = Fumonisins DON = Deoxynivalenol
Immune Suppressive

 Aflatoxin
 Vomitoxin,
 T - 2 toxin
 Ochratoxin
 Fumonisins
 研究和开发

選擇材料與納米結構特點
 How do mycotoxins act?

2.Absorp Mycotoxin at intestine

Impact to production and transform at liver

Health ,Immunity

x xx
x x
x

1.Mycotoxin Mycotoxin destroy target organ

contaminate in feed and be deactivated
by liver kidney
 Our Learning of Mycotoxin
1. Polar Mycotoxin can absorped by Clay and Yeast Cellwall: Aflatoxin
2. Non Polar Mycotoxin ,have some surface charge can absorped by
clay and Yeast Cellwall but need to create environment : Zeralenone
3. Non Polar Mycotoxin ,No surface charge need to change
Mycotoxin’s structure for more polarity and synergistic effect in
nano level (Nanocomposit action): Fumonisin /DON /Ochatoxin
4. We have to absorp Mycotoxin As much as Posible
before get into liver
5. Liver and Kidney be Performed for Eliminate Mycotoxin
6. Mycotoxin have > 200 type and combination effect need
to manage as “Broad Spectrum effect”

24
Zetatechnology L Tonic

 We develop Nanocomposite
by enhance the absorption
performance and support
Enzyme’s Immobilization
support Biotransformer action
 Totally, we are the first Nanocomposite
plus Biotansformer in Mycotoxin
binding action
 Our products are Theurapeutic
Program planning for Feed
Industry (Prevent +Treatment
before lose)

25
Zetatechnology L Tonic

26
 Select Raw Material : Characterization
Zeta
18
16

Volume @ STP (cc/g)

14
12
10
8
6
4
2
0
0.000000 0.200000 0.400000 0.600000 0.800000 1.000000 1.200000
Relative Pressure (p/p0)

Surface Area Specification of Zeta L-Tonic®

Product Characteristic of Surface area

Average pore size Total No.Pore Type of pore
BET Surface area
小孔尺寸 小孔总数 IUPAC
面积
(Nanometer) (cc/g)
[m2 /g]

Zeta L-Tonic® 9.161 4.091 0.028 Mesopore

 Mode of Action of Zeta technology

1 Attraction 2 Absorption

Polar mycotoxins
(Aflatoxin, CPA, Ergot)
Adsorbent Liquid phase
Na+ O O-CH3
O
O
O Pore system:
Ca++ O • Macro
ß-ceto-lactone of
• Meso
Aflatoxin
• Micro
• Submicro

Non-polar mycotoxins
(DON, FB1, OTA, T-2, ZEA)
Zetatechnology L Tonic

29
 Plant Protease Mechanism

Plant protease mechanisms, Ochratoxin A can be cleaved by enzymatically (a).

Enzymes that catalyze the hydrolytic cleavage of Ochratoxin A are called plant
protease. In the active sites of this plant protease , the eponymous residue is
usually paired with a proton-withdrawing group to promote nucleophilic attack on
the Ochratoxin A. Close-up on the action, three amino acids located near the
active site (Asn175, His259, and Cys25) are labeled
(b). Finally, products are released, and enzyme is free to bind other Ochratoxin A
molecules (c).
30
Select material by Characteristic’s Nano structure
Zetatechnology
Normal Clay Modified Clay Zetatechnology
+Biotransformer
Charateristic
Surface Area m2/g 4.643 14.62 14.62
pore diametre nm 5.3 10-54.58 16.5 16.5
Total pore cm3/g 0.56 0.1267 0.6032 0.6032
% Absorption
Aflatoxin* 85 90-100 90-100 90-100
Zearalenone* 30 85 85-100 85-100
Fumonisin* - 40 85-100 85-100
Ochartoxin** - 40 75 85-100
Dosage per ton (KG) 3-5 1-3 1 1
* Ku Vet lab ** Central lab
 Select & Develop Raw Material
Nano structure

Pore size 孔尺寸

Big Pore Pore volume 孔总数
Pore 小孔
大孔 Zeta potential

Clays HSCAS Modified Clays Nano composite

www.themegallery.com
 Select & Develop Raw Material
Nano structure

Pore size 孔尺寸

Pore volume 孔总数
Pore Big Pore Zeta potential

Clays HSCAS Modified Clays Nano composite

www.themegallery.com
 Select & Develop Raw Material
Nanocomposite testing
Nano structure
:

Normal Clay
Nanocompozite Modified Clay Nanocompozite
粘土
改性粘土 +Biotransformer
2.Enzymatic Detoxification &
Biotransformer
 Enzymatic Biotransformation
酵素生物转化
 It is the detoxification of Mycotoxins through
Biotransformation by enzyme produce by
microorganism.
 The mycotoxin molecule is breakdown into more simple
molecule简单的分子and less toxic as compare to the
original form.
 The key to Enzymatic Biotransformation is the right
amount of enzyme unit present

毒 毒性减少

Ezymatic Biotransformation
 Enzymatic Detoxification
酵素生物转化

% Bio transformation
Enzyme Dosage 生物转化率
用量
Zearalenone Fumonisin B1 Ochratoxin A
玉米赤霉烯酮毒素 伏马菌素 赭曲霉毒素

50 mg/10 mL
nd. nd. nd.
Mycopase®

100 mg/10 mL
nd. nd. 25.43%
Mycopase®
 Biotransformer & Nanocomposite Performance

Zeta L-Tonic®

Product Dosage % Absorption 吸附率

用量 Fumonisin B1
Zearalenone Ochratoxin A
玉米赤霉烯酮毒素 伏马菌素 赭曲霉毒素

Zeta L-Tonic® 100 mg/10 ml 82.7% 83.5% 90.1%

Our Learning of Mycotoxin

40
ซีต้าเทคโนโลยี : นวัตกรรมสารดูดจับชีวพิษเชื้อรา
 Zeta CARE in support Environment

Herbal Technology 1-3 ,1-6 Betaglucan

Support Liver Function Immuno-stimulation
Feed Intake
Structural Absorption

Organic
Glycine Chelate
Functional
Supplement

Zn Zeta Oxidant
Mn Diatomateus Earth
Cu Natural Essential oil

www.themegallery.com
 Zeta CARE : Glycine Chelate

 Zn Chelate
Zn, Mn, Cu absorbtion of Ca
 Increased
 Egg shell quality
 Immune enhancer
Zn  Pigment of egg shell
 Mn Chelate
Mn  Improved fertility and bone structure
Cu  Cu Chelate
 Pigment of egg shell
 Immune enhancer
 Key Success of Zeta technology

1 2 3

Composition Mode of Action Solve Key

1 Nanocomposite 1Fast absorb before
2 BioTransformer transform in liver Mycotoxin
3 Support Liver 2 Bio Transformer
Kidney Function Laboratory &
3 Support Liver Kidney
Function Field result

Fast Action 快操作

www.themegallery.com
 KLEAN Greentech Mycotoxin

Nano Composite Zeta-CARE

Y Y

Y Y Y Y Y Y Y Y Y Y

Y YY Y YY Y YY Y YY Y YY

Y YY Y YY Y YY Y YY Y YY

Y Y Y Y Y Y Y Y Y Y
45
 Why Zeta-Technology Work !!

Zeta Bio 1-3 ,1-6 Beta Zeta % Absorption

Zn, Mn, Cu
Composite Transformer glucan Care 吸附率

Zearalenone +++ ++ ++ +++ 80-100%*

Fumonisin +++ + ++ ++ 83-100%*

Ochatoxin +++ +++ + ++ 90%**

Aflatoxin +++ +++ + +++ 84%*

* Veterinary Sci Faculty ,Kasetsart University ,2014

* * Central lab ,2014

Source: Central lab ศูนย์ห้องปฏิบตั ิการกลาง ,สิ งหาคม 2556
Mycotoxin Binding CapacityAnalysis
黴菌毒素的吸附能力

Veterinary Sci Faculty ,Kasetsart University ,2014

 Comparison of different Mycotoxin Binder on
Zearalenone & Fumonisin 不同黴菌毒素吸附劑的比較

Absorption pH 6 (%)
Dose kg/ton Zearalenone Fumonisin B1
用量 200 ppb 1000 ppb
Comp Ags 2 67.31 62.04
1 67.35 23
Comp MF 1 7.28 14.42
Comp MT 1 1.84 45.02
Zeta ZAD 2 87.5 98.5
1 81 75
Zeta TOF 2 87.5 95.5
Zeta L Tonic 1 76.76 66.67
1 73.71 65.72
0.5 61.7 51.51
Zeta Plus 2 57 60
1 33.5 45.46
Zeta 8 2 57 60

Central lab ,2014

52
ADD VALUE SERVICE额外服务

原料检测
找到快解决方案
 Raw Material Risk
Low Risk Moderate Risk High Risk
1. % Mycotoxin Positive Less than 25 % positive 25 - 75 % More than 75 %
)

2. Mycotoxin toxic Level Less 100 % 100 - 150 % more than150 %

3.% CV Sampling Random less 10 % 10 - 35 % More than 35 %
4. How long of Stock Less than 7 day 7 - 10 วัน More than 7 - 10 day
(ที่มา : Cargill Animal Nutrition , Romer labs )
Theurapeutic Program Planning: KGT Tools
Therapeutic Program Planning TPP
與KGT的工具治療規劃方案
Normal
Loss before TPP
AVERAGE DAILY GAIN

Infection
TPP

Treatment
Profit from TPP
Profit from Treatment

Death
TIME IN DAYS
 Dosage of Zeta Products

 Preventive dose: 0.5 kg/t

 Standard dosage: 1.0 kg/t

 In case of serious problems:

Small Animal : up to 2.0 kg
Breeder : up to 1.5 kg
 Thank you for your Supports
National Innovation Award 2014
Economic
Zetatechnology : Innovative Mycotoxin Binding
Innovator : Dr Kitti Supchukun
Dr Kittiwut Kasemwong
 ผลงานวิจยั และพัฒนาของ ศน.
ได้ร ับรางวัลในระด ับชาติและนานาชาติ
National Innovation Award 2014 Seoul International Invention Fair 2014
“Honorable Mention in Economics Award” “Silver Prize”

ดร. กิตติวุฒิ เกษมวงศ ์ หัวหน้าห้องปฏิบต ิ ารนาโนเพื่อผลิตภัณฑ ์อาหารและการเกษตร

ั ก
VIII International Warsaw
Invention Show 2014 ร่วมกับ บริษทั คลีนกรีนเทค จํากัด ศึกษาคน้ ควา้ วิจยั และพัฒนาผลิตภัณฑ ์นวัตกรรมดูด
“Special Award”
้
จับสารพิษจากเชือราปนเปื ้ อนในวัตถุดบ
ิ อาหารสัตว ์ (Mycotoxin binder) ภายใตช ื่
้ อทาง
การค ้า ZETATECHNOLOGY ™ ผลิตภัณฑ ์ดังกล่าว ไดร้ บั รางวัลนวัตกรรมแห่งชาติปี
๒๕๕๗ รางวัล ชมเชยด า้ นเศรษฐกิ จ นอกจากนั้ นยังได ร้ บ ั รางวัลในเวที ก ารประกวด
่
ผลงานวิจยั และสิงประดิ ษฐ ์ระดับนานาชาติ ไดแ้ ก่ รางวัลพิเศษจากงาน “International
Warsaw Invention Show 2014” (IWIS 2014) โดยสมาคมนั กประดิษฐ ์ประเทศ
่ นที่ ๑๔-๑๖ ต.ค. ๒๕๕๗ ณ กรุงวอร ์ซอ ประเทศโปแลนด ์ และรางวัลเหรียญ
โปแลนด ์ เมือวั
เงินจากงาน “Seoul International Invention Fair” (SIIF2014) โดยสํา นั กงาน
ทรัพย ์สินทางปัญญาแห่งสาธารณรัฐเกาหลี (KIPO) ณ กรุงโซล สาธารณรัฐเกาหลีใต ้ เมื่อ
วันที่ ๒๗-๓๐ พ.ย. ๒๕๕๗