Ingredient For Anti-Pollution PDF

Anti-pollution
with
19th. JUNE. 2015

Pollution
Pollution
World Carbon Dioxide Emissions by Country
Mongabay.com
Source : EIA 2005
Pollution
Health Eeffects of pollution

Air pollution
Air pollution Water pollution
Soil Contamination
Pollution and Damages
Air pollution Damages

Sulfur dioxide DNA damages
ATPase inactivation
Ozone Oxidative stress

MMP/ HSP
Airborne particles Pigmentation
Particulate matter
Oxidative stress
Soot aerosol Oxidative stress

Smoke of Cigarette Aging
Nitrogen oxide DNA damages

Aging
UV radiation Oxidative stress

DNA damages
Sulfur dioxide
Skin contact
Breathing SO2 stinging pain, redness of the
Irritation to the nose skin and blisters
High level of SO2
and throat
could give the
adverse health effects
to a person
who be exposed
in the Cell?
Eye contact
Watering and in severe cases
blindness can occur
SO2 is colorless gas with an irritating pungent odor

It is a common air pollutant from the burning of coal and oil
Sulfur dioxide in a cell
DNA damage DNA damage

Chromosomal aberrations
ATPase inactivation Sister chromatid exchange &
micronuclei
Oxidative damages
Ozone
The first target of Ozone in the skin

Superficial epidermal layer, Stratum corneum
The depletion of anti-oxidants such as
Vitamin E and Vitamin C in the stratum
corneum AGING
The next target of Ozone in the skin
Induction of Redox Sensitive Transcription Factors
Induction of Heat Shock Proptein(HSP) and Inflammatory Makers
Induction of Matrix Metalloproteinase(MMPs)
Ozone is an inorganic molecule with the chemical formula O3 and a pale blue gas with a distinctively pungent smell
It is a powerful oxidant and causes to damage mucous and respiratory tissues
Ozone can induce the skin aging like as ROS
Airborne particles
Particulate matter
Hypothesis
Long-term exposure to air pollution might lead to
extrinsic skin aging through oxidative stress
generated by the particles themselves or by
associated PAHs(Polycyclic Aromatic
Hydrocarbons)
PAHs induce melancyte proliferation and thereby skin

pigmentation
PAHs are converted into quinones, redox-
cycling chemicals that produce ROS
Long term-exposed skin to PAHs either
through hair follicle or transepidermal absortion
may lead to oxidative stress and skin aging
PAHs can lead to acneform eruption
Extrinsic Aging
Soot aerosol Pigmentation & aging

Smoke of Cigarette
Transepidermal water loss
Degeneration of connective tissue
Increase of matrix metalloproteases(MMP-1, MMP-3)
Deeper periorbital wrinkling
Orange-purple skin discoloration
Psoriasis
Disturbing balance of oxidant and
anti-oxidants by ROS
Acnes
Extrinsic Aging
Cigarette smoke is a highly complex aerosol composed of thousands of chemical substances,

including ROS, RNS and electrophilic aldehydes.
Nitrogen dioxide
Nitrogen dioxide is a gas of reddish-brown
color with a distinct sharp and biting odor
Combustion of fossil fuels produces
both NO2 and NO
NO2 and NO can destroy

organic matter, ex. human tissue
and lead aging
UV radiation
UVBis largely absorbed by epidermal cellular components.
UVA can penetrates deeply into the basal layer of the epidermis and dermal fibroblast.
Cutaneous immunosuppression and skin cancers
Photoaging:
extringic aging
Pollution and Solution
Air pollution Damages

VB9-plus DNA damages
Sulfur dioxide
Hydracell Q ATPase inactivation
Ozone F. Shiunko Oxidative stress

Cera-Shea ST MMP/ HSP
Airborne particles F. Shiunko Pigmentation

Particulate matter
Hydracell Q Oxidative stress
Soot aerosol BIO-Placenta Oxidative stress

Smoke of Cigarette Cera-Shea ST Aging
DNA damages
Nitrogen oxide F. Shiunko
VB9-plus Aging
Oxidative stress
UV radiation A.-proVita D3
VB9-plus DNA damages
Pollution and Solution
Recommendation from DAMY

Expected efficacy Product
Cleansing Fermentoil olive
Hydration Hydracell Q
Cleansing
Skin soothing VB9-plus
Anti-irritation 888
Moisturizing Hydracell Q
Enhancement of skin barrier Ceramide-Shea butter ST
Skin Care
BIO-Placenta
Anti-wrinkle
Fermentoil Shiunko
Skin soothing VB9-plus

Anti-irritation 888
Protection Enhancement of skin barrier Ceramide-Shea butter ST
Aqua-proVita D3
Protection from skin damages
Fermentoil Shiunko
The Quality of Plant Oil KR Patent / PCT Pending
Fermentoil Olive / Argan / Green tea seed / Macadamia seed oil / Jojoba seed oil /
Sunflower seed oil / Safflower seed oil / Dongbaek seed oil
New generation of plant oil for developing your cosmetics
Plant oil fermentation increases nutritional values, improves skin feeling, absorption and
compatibility for the cosmetic products through the microbial fermentation system
COPYRIGHT © DAMY CHEMICAL CO., LTD. ALL RIGHTS RESERVED.

Oil & Cosmetics
Organic
Mineral
Oil
Fermentoil
Water Silicon
Plant
Synthetic
Choosing the best oil Creating new category of oil

can make a difference through unique fermentation
in your formulation technology
Oil Fermentation Technology
Pseudozyma sp. SY-16[KCTC 8950P]
• Odorless in aerobic condition

• It changes the substance in plant oil by producing enzymes
• It produces various types of polysaccharides and lipids
• KRIBB have researched to develop organisms that can

clean the waste oil in the ocean
• They applied in coastal area(Tae-an, Korea) to decompose
the oil leakage in 2009
The patent was registered through cooperation with Damy and

Korea Research Institute of Bioscience & Biotechnology
Fermentation Process of Fermentoil
Glycerol
Triglyceride
H
Fatty Acid
H H C OH
O
H C H C OH
O
H C O H C OH
H C O H
O
H
O
“Free” Fatty Acid
O-
Attack
point O
O-
Catabolism
lism O
a b o
An O-
MEL Pseudozyma sp.

SY-16[KCTC 8950P]
Linoleic acid
Linolenic acid
CLICK
Surface tension
36
36
Surface tension (dyne/cm)
34
34
32
32
30
30
28
28
26
26
24
24
0 0 2
2 44 66 8 8 10
10
MEL concentration in olive oil (%,v/v)

Analysis of Free fatty acid Value
Detection method 2D-TLC Analysis
2nd Solvent Development Olive oil Fermentoil Olive
1st Solvent Development
Comparing to the result of TLC Analysis,

the amount of free fatty acid increased after fermentation
Detection method Metrohm Titrato
Olive oil Fermentoil Olive
Olive oil Fermentoil Olive

KOH(mg/g) 0.13 11.84
Comparing to the result before fermentation,

the amount of free fatty acid increased by 90 times
Detection method HPLC Analysis
Olive oil
50% Olive oil
Oleic
acid
Linoleic
acid Stearic
acid
Palmitic
acid
Fermentoil Olive
10% Fermentoil Olive
Linoleic acid Palmitic acid Oleic acid Stearic acid

Olive oil 0 0 374.4 0
73.3
Fermentoil Olive 5978.3 4740.7 27457.0 1668.3
(unit: ppm)
2nd Solvent Development Argan oil Fermentoil Argan

Argan oil Fermentoil Argan
Argan oil Fermentoil Argan

KOH(mg/g) 0.64 5.81

Argan oil
50% Argan oil
Linoleic Oleic
acid acid
Stearic
acid
Palmitic
acid
Fermentoil Argan
10% Fermentoil Argan

Argan oil 1240.3 378.7 1160.6 262.7
13.6 14.9 18.1 5.9
Fermentoil Argan 16926.3 5628.0 21053.3 1549.0
(unit: ppm)
Green tea seed oil Fermentoil GTS

2nd Solvent Development


KOH(mg/g) 0.21 15.38

Green tea seed oil

50% Green tea seed oil
Linoleic Oleic
acid acid
Palmitic Stearic
acid acid
Fermentoil GTS
25% Fermentoil GTS

Green tea seed oil 234.7 243.6 0 0
40.68 19.66
Fermentoil GTS 9549.193 4790.789 31031.54 4989.309
(unit: ppm)
Morphology Change
Morphology Change
Detection method Optical microscope
Oil
Oil
Oil
Oil
ㄹ Pseudozyma Oil Feeding(0hr) Fermention(60hr) Fermentation(120hr)
Magnification : X1000
Morphology of oil particle was observed during fermentation process by optical microscope
Improved Emulsifying Capacity
Olive oil
pH9.0 pH7.0 pH5.0
Fermentoil
Olive
pH9.0 pH7.0 pH5.0
The emulsifying capacity of Fermentoil Olive can be showed

in a wide range of pH comparing to other olive oil
Improved Anti-oxidative Effect
Anti-oxidative Effect
DPPH assay
*RSA : Radical Scavenging Activity
100 100 100

75 75
RSA(%)
RSA(%)
75
RSA(%)
50 50 50
25 25 25
0 0 0
1 10 5 10 5 10 (%) 1 10 5 10 5 10 (%) 1 10 5 10 5 10 (%)
Vit E Olive oil Fermentoil Vit E Argan oil Fermentoil Vit E GTS oil Fermentoil
Olive Argan GTS
Fermentoil Olive Fermentoil Argan Fermentoil Green tea seed
100 100 100

RSA(%)
75 75
RSA(%)
75
RSA(%)
50 50 50
25 25 25
0 0 0
1 10 1 10 1 10 (%) 1 10 1 10 1 10 (%) 1 10 1 10 1 10 (%)
Vit E Meadowfoam Fermentoil Sunflower oil Fermentoil Vit E Dongbaek Fermentoil

Vit E
seed oil Meadowfoam seed Sunflower seed oil Dongbaek seed
Fermentoil Meadowfoam seed Fermentoil Sunflower seed Fermentoil Dongbaek seed
The overall RSA of oil is improved after fermentation

Advantage of Fermentoil
Non-greasy Feeling
Skin Absorption Free fatty acid value
Regular Plant Oil
Fermentoil
Linoleic, linolenic acid value

Odorless & Colorless
Emulsifying Capacity
General plant oil has been improved in quality and compatibility

through Damy’s fermentation technology
History & Benefits
Olive oil
Recommended for dry skin
Material Benefits Story

• Oleic acid • Moisturizing • Olive oil is used in traditional
• Vitamin E • Anti-aging medicine and food from
• Polyphenol • Antioxidant activity 6,000 years ago
• Protection against UVB damage • Olive oil used for Fermentoil
• Antio-inflammation Olive is grown with fertile soil

and rich sunlight of Italy
History & Benefits
Argan oil
Recommended for sensitive skin

• Linolenic acid • Moisturizing • Called as a ‘Golden liquid presented
• Oleic acid • Anti-sebum from God’
• Vitamin E • Anti-aging • Popular oil for hair care with its nutrition
• Polyphenol • Wound healing • Rigidly selected Argan grown under
• Anti-acne optimal conditions of Morocco

• Valuble oil that only 1 liter is obtained
from 7 trees
History & Benefits
Green tea seed oil

Recommended for oliy and acne skin
25ml
800 green tea seeds

• Unsaturated fatty acid • Moisturizing • Green tea seed is praised as the beauty
• Vitamin E • Antioxidant activity secret of the Geishas.
• Green tea seed is recognized as very
• Polyphenol • Antiseptic
• Fungicide and bactericide precious oil in Eastern Asia
• It is valuble oil that only 25ml is obtained
from 800 green tea seeds

History & Benefits
Meadowfoam seed oil


• Eicosenoic acid • Moisturizing • ‘Meadowfoam’ received its name because
• Oleic acid • Antioxidant activity of its resemblance while in bloom to the
• Linoleic acid • Skin nutrition white foam in the meadow blowing on the
• Vitamin E • Skin rejuvenating ocean.
• Anti-aging • Meadowfoam seed has been studied for
• Recovery of hair damages pharmaceutical use as well as cosmetic

industry because of its medicinal efficacy.
• Primium oil which contains high contents of
long chain fatty acid.

History & Benefits
Macadamia seed oil

Recommended for aged skin

Skin barrier enhancement
• Macadamia seed oil have used by Austrailia natives
• Oleic acid •
Anti-aging from thousands years ago for food

• Palmitoleic acid •
• Macadamia seed contains high content in Palitoleic
• Vitamin E • Antioxidant activity
• Wound healing acid, a monounsaturated fatty acid that avoids
oxidation.Palmitoleic acid is found in human sebum
among young, but the level dramatically drops in
mature skin. Palmitoleic acid makes it a botanical
alternative to mink oil.
• Macadamia seed oil is called ‘Vanishing oil’ due to its
good skin absorption

History & Benefits
Sunflower seed oil


• Linoleic acid • Moisturizing • Sunflower seed has been used by
• Phytosterol • Antioxidant activity American Indian tribes for body
• Vitamin E • Improvement of skin irritation painting, decorations, ointments, skin
• Polyphenol • Bactericide and hair care.
• Sunflower is a plant which has strong
self purification capacity ; Sunflower

was used for purifying contaminated
wastewater site in Ohio and removed
more than 95% of uranium in 24h
(Dushenkov et al., 1997 a,b, 1995)
History & Benefits
Safflower seed oil

Recommended for dry skin

• Linoleic acid • Moisturizing • Safflower has been traditionally used
• Vitamin E • Antioxidant activity for fabric dying in Korea.
• Antiseptic • Safflower has been used for color
• Fungicide and bactericide makeup especially on lips and
cheeks by women of Joseon
dynasty.
• Safflower seed is used as food oil.
History & Benefits
Dongbaek seed oil

Recommended for dry skin and hair care

• Dongbaek blooms in winter.
• Oleic acid • Moisturizing • Dongbaek seed is
• Camellin • Antioxidant activity traditionally used for hair
• Camelia saponin • Conditioning effect nutrition and hair polish in
• Improvement of skin irritation Korea.
• 7 Dongbaek forest are
designated as Korean natural

monument.
Painting : Shin Yunbok, Dano

The Quality of Plant Oil
KR Patent / PCT Pending
Korean Medicinal Herb Angelica
Artemisia
Fermentoil Series
Red ginseng
Glycyrrhiza
Leonurus
New generation of plant oil to improve your cosmetics Saururus
Shiunko
Benefits of Korean medicinal herb are added in Fermentoil Series.

Advantage of Korean Medicinal Herb Fermentoil
Olive oil Fermentoil Angelica

• Moisturizing
• Antioxidation activity
Improved
DAMY Fermentation
• Protection against Fermentoil Artemisia
UVB damage
• Anti-inflammation Application
in cosmetics Fermentoil Red ginseng
Non-greasy Feeling Fermentoil Glycyrrhiza

Improved Skin Absorption
Improved Emulsifying Capacity Fermentoil Leonurus
Improved compatibility
Korean Increased beneficial free fatty acid value
Fermentoil Saururus
medicinal
herb Fermentoil Shiunko
History & Benefits
Angelica gigas

• Nodakenin • Anti-wrinkle • Angelica has been used as a medicine
• Decursin • Anti-oxidation for
• Isoimperatonin • Anti-inflammation more than 2000 years.
• Anti-microbes • Angelica is known to help nourishing of
• Moisturizing the blood according to Dongeuibogam*
* Dongeuibogam(東醫寶鑑) : Korean traditional medicinal book written in Sun Dynasty, 1596~1610 (National Treasure)
History & Benefits
Artemisia princeps

• Polyphenol • Anti-microbes
• Artemisia describes in the
• Anti-inflammation myth of the Dangun.
• Artemisia princeps is
• Anti-oxidation
• Brightening mentioned more than 500

times as prescription in Dong-
eui-bo-gam*
• Wide range of use in Korea ;
Insect repellent, spa, food and

skin care
Painting : Shin Yunbok, Dano

History & Benefits
Panax ginseng
Recommended for sensitive and aged skin
1000 years

• Ginsenoside Rg1 • Wound healing • Panax ginseng has been used from
• Hydrating
• Ginsenoside Rb1 1000 years ago.
• Anti-inflammation
• Panax ginseng has known to help
• Anti-stress
recover one’s energy according to
• Collagen synthesis
Dongeuibogam*
History & Benefits
Glycyrrhiza uralensis

• Anti-oxidation • Glycyrrhiza uralensis has been used
• Glycyrrhizin
• Anti-inflammation with most medicinal herbs together for
• Liquirtin
• Anti-irritation its multi-functional effect in Korean
• Brightening traditional prescription.
• Glycyrrhiza uralensis has been known
to help promotion of blood circulation,

detoxification according to
Dongeuibogam*
History & Benefits
Leonurus sibiricus
Recommended for aged and dehydrated skin

• Oleic acid • Anti-oxidation • The Korean herb name
• Linoleic acid • Anti-aging ‘Ikmocho’ means “Good for
• Leonurin • Anti-microbes mother”.
• Saponin • Wu Tse-t’ien, the first woman
emperor of China used it for

beauty.
• Leonurus sibiricus is known to
have efficacy for pregnancy.

History & Benefits
Saururus Chinensis
Recommended for aged and dehydrated skin

• Quercetin • Anti-oxidation • There are only 2 genus in
• Quercitrin • Brightening Saururus species
• Methyl-n-nonyl-ketone • Soothing • Native plant only grown in Jeju
• Anti-microbes island
• Saururus Chinensis is known
to help detoxification
History & Benefits
Shiunko

• Z-ligustilide • Anti-oxidation • Shiunko is traditional ointment
• Shikonin
• Anti-microbes which is made of Angelica
• Proliferation sinensis and Lithospermum
• Collagen secretion erythorhizon.
• Anti-inflammation • The dying of Lithospermum
erythorihizon has been loved

as a symbol of emperor and
elegance from 2000 years
ago.
Fermentoil Shiunko
Biotechnology + Traditional ointment = Fermentoil Shiunko
Concept
Angelica gigas
Shiunko Olive oil
Traditional prescription
for medicine Lithospermum erythorihizon
Wound healing
Fermentation Anti-inflammation
Improved Application Allergy relieve
in cosmetics
Anti-oxidation
Whitening
n i n
Fermentoil h i ko
S
Shiunko
Anti-oxidative effect
Detection method Folin-Ciocalteu Assay
Gallic acid
Gallicacid standard Curvecurve
standard Total Phenolic contents
Total Phenolic contents (mg GAE/g)

2.5000
0.6
2.0000 y = 3.8838x - 0.0601

R² = 0.9918 0.45
1.5000
O.D. (760nm)
(760nm)
1.0000
0.3
O.D.
0.5000
0.15
0.0000
0 0.1 0.2 0.3 0.4 0.5 0.6
0
Fermentoil Fermentoil Fermentoil
-0.5000
GA (mg/ml) Oive GTS Shiunko
Polyphenol
Test sample
(Galic acid Contencs)
Fermentoil Olive 0.21 mg GAE/g
Fermentoil GTS 0.23 mg GAE/g
Fermentoil Shiunko 0.59 mg GAE/g
Significant synergic effect between Paeonia lactiflora flower extract and Arbutin
Fermentoil Shiunko shows highest contents in Polyphenol contents
has been found on reduction of Melanin contents
Detection method Folin-Ciocalteu Assay
This method is based on the reduction of a

phosphomolibdic-tungstinic chromogene by an
antioxidant(Fermentoil Series) and a change of
color with the absorbance measured at 750nm
using a spectrophotometer.
Folin-Ciocalteu reagent reacts to give a Yellow Color Blue
characteristic blue color. It is used for detection
Polyphenol
of polyphenol. The antioxidant activity is
expressed as the number of equivalents of Anti-oxidative
effect
polyphenol.
Reference : http://en.wikipedia.org/wiki/Folin–Ciocalteu_reagent
Whitening effect
Melanin contents
Melanin contents (% of control)

250 α-MSH (100nM)
200
150
100
50
0 l
SH
25
5
tin
ro
0.
0.
0.
bu
nt
-M
Co
at
M
%
0n
01
Alcohol extract of Fermentoil Shiunko (%)

10
0.
NOTICE
Cells survive with water.
As Fermenotil Shiunko is one of the
oil, when Fermentoil Shiunko is
treated as a sample in-vitro test,
the layer is seperated into water
and oil phase.
0.1% 0.25% 0.5% Thus, in order to obtain more
0.01%
Control 𝒶-‐MSH accurate result, the sample has
Arbutin Alcohol extract of
Fermentoil Shiunko been prepared through alcohol
extract method from Fermentoil
100 173.932 141.88 191.026 147.009 54.2735 Shiunko.
Significant synergic effect between Paeonia lactiflora flower extract and Arbutin
Alcohol extract of Fermentoil Shiunko has shown whitening effect
has been found on reduction of Melanin contents
DPPH assay
*RSA : Radical Scavenging Activity
100
75
RSA(%)
50
25
0
1 10 1 10 1 10 1 10 1 10 1 10 1 10 1 10 1 10
Vit E Fermentoil Fermentoil Fermentoil Fermentoil Fermentoil Fermentoil Fermentoil Fermentoil
Olive Angelica Artemisia Red ginseng Glycyrrhiza Leonurus Saururus Shiunko
Concentration(%)
The overall RSA of oil is improved after fermentation

Summary
Fermentoil Series
Recommended
Fermentoil Series INCI name Benefits & Uses Dosage
Pseudozyma epicola / soybean flour /

Fermentoil Olive
olive fruit oil ferment filtrate

Fermentoil Argan
Argania spinosa kernel oil ferment filtrate
Pseudozyma epicola /camellia sinensis seed oil / glucose/

Fermentoil Green tea seed glycine soja (soybean) meal / malt extract / yeast extract
ferment filtrate
Fermentoil Meadowfoam Pseudozyma epicola / soybean flour / meadowfoam seed oil - Non-greasy feeling
- Improved skin absorption
seed ferment filtrate Up to 99%,
- Improved emulsifying capacity
- Improved compatibility
depending on
- Increased beneficial free fatty
formulation
Pseudozyma epicola / soybean flour / macadamia seed oil
Fermentoil Macadamia seed acid value
ferment extract filtrate
Pseudozyma epicola / soybean flour / sunflower seed oil

Fermentoil Sunflower seed
Pseudozyma epicola / soybean flour / safflower seed oil

Fermentoil Safflower seed
Pseudozyma epicola / soybean flour / camellia japonica seed

Fermentoil Dongbaek seed
oil ferment extract filtrate
Summary
Korean Medicinal Herb Fermentoil Series

Korean Medicinal Herb Recommended
Fermentoil Series INCI name Benefits & Uses Dosage

Fermentoil Angelica apricot kernel oil / olive fruit oil / sunflower seed oil / sweet
almond oil / angelica gigas root extract ferment extract filtrate

Fermentoil Artemisia apricot kernel oil / olive fruit oil / sweet almond oil / sunflower
seed oil / artemisia princeps extract ferment extract filtrate

Fermentoil Red ginseng apricot kernel oil / olive fruit oil / sweet almond oil / sunflower
seed oil / panax ginseng root extract ferment extract filtrate
- Basedon the benefits from

Pseudozyma epicola / soybean flour / Up to 99%,
Fermentoil, adding functional
Fermentoil Glycyrrhiza apricot kernel oil / olive fruit oil / sunflower seed oil / sweet
elements from each Korean
depending on
almond oil / licorice root extract ferment extract filtrate formulation
medicinal herb

Fermentoil Leonurus apricot kernel oil / olive fruit oil / sunflower seed oil / sweet
almond oil / leonurus sibiricus extract ferment extract filtrate

Fermentoil Saururus apricot kernel oil / olive fruit oil / sunflower seed oil / sweet
almond oil / saururus chinensis extract ferment extract filtrate
Pseudozyma epicola / soybean flour / apricot kernel oil / olive

fruit oil / sunflower seed oil / sweet almond oil / (angelica
Fermentoil Shiunko
gigas / lithospermum erythrorhizon) root extract ferment extract
filtrate
Anti-aging, Skin rejuvenation
BIO-Placenta Pseudo-Placenta with synergic effects and safety

Contains five different growth factors (EGF, IGF-1, acidic FGF, basic FGF and VEGF), amino
acid and vitamin. These growth factors are safely synthesized.

Why Placenta?
Commonly known effects of

dermatological efficacy of the placenta
are Anti-aging & Skin rejuvenation More than 18 years old
GF production and the
growth process
decreases rapidly.
Minimal amount is
produced for
0~3 years old metabolism.
Infants produce
nutritional values
and GF on their own.
GF decrease and
absent of cell
replacement leads to
aging.
10~13 years old
GF production
decreases in body
and growth rate
Fetus starts to slow down.
Fetus obtains all the
nutrients and GF
made by placenta.
Aging is caused by loss of

GF will stimulate the
expansion of cells in
the body system.
Growth Factors(GF)
Reference: Placental growth factors, B.V.Rama Sastry, Placental pharmacology, p126~150

Summary of Advantages
BIO-Placenta VS Human Placenta

Components of BIO-Placenta are similar to Human Placenta which is based on
‘Placental Pharmacology’
Synthesized Growth factors

Components are safely synthesized
Proven to contain Growth Factors

The inclusion of growth factors can be found by HPLC analysis
Synergic Effect
Combination of 5 growth factors and essential factors make synergic effect on skin
Advantages
BIO-Placenta VS Human Placenta

Human Placenta Effect BIO-Placenta
EGF Skin rejuvenation EGF
IGF-1 Anti-wrinkle IGF-1
acidic FGF Anti-wrinkle acidic FGF

Growth
basic FGF Elasticity enhance basic FGF
Factor
VEGF Nutritional supplement VEGF
TGF-β Anti-wrinkle -
The minority parts - -
Vitamin Cell vitalization Vitamin : Vitamin B9

Essential
Factor Amino acids Energy supplement
Amino acids : Acetyl
Glutamine
Reference: Placental growth factors, B.V.Rama Sastry, Placental pharmacology, p126~150
Components of BIO-Placenta are similar to human placenta

Advantages
Manufacturing Process of Growth factors

EGF DNA sequence
5‘ GTT ACC AGC GAC TCC GAA TGC CCG CTG AGC CAT GAC GGC
3‘ CAA TTG TCG CTG AGG CTT ACG GGC GAC TCG GTA CTG CCG
TAC TGC CTG CAC GAC GGG GTA TGC ATG TAC ATC GAA GCA
ATG ACG GAC GTG CTG CCG CAT ACG TAC ATG TAG CTT CGT
CTG GAC AAA TAC GCG TGC AAC TGT GTT GTT GGC TAC ATC
GAC CTG TTT ATG CGC ACG TTG ACA CAA CAA CCG ATG TAG
GGC GAG CGC TGT CAG TAC CGT GAC CTT AAG TGG TGG GAA
CCG CTC GCG ACA GTC ATG GCA CTG GAA TTC ACC ACC CTT
n
atio
CTG CGC TGATAACCTGCA 3’
GAC GCG ACTATTGG 5’.
Growth factors
rific
Synthesis of gene by PCR
Pu
EGF
IGF-1
Acidic FGF
Proliferation Basic FGF
Injection VEGF
Fermentation
Recombinant E.coli
Components are safely originated from microbial fermentation

Advantages
Proven to contain Growth Factors
EGF
aFGF
BIO-Placenta
bFGF
Product ‘L-’
Commercial human placenta products
5" 10" 15" 20" 25" min"
Different from commercial human placenta products,

the inclusion of growth factors can be found by HPLC analysis.
Advantages
Synergic Effect
Blank EGF+IGF-1
EGF
acidic
IGF-1 FGF
Essential
Factors
basic
VEGF
FGF
IGF-1 EGF
BIO-Placenta
Anti-wrinkle
Fig. Effect of growth factor singling on wound

Skin rejuvenation
epithelialization.
Ref; Journal of Cell Science 116, 3227-3248
Combination of 5 growth factors and essential factors make synergic effect on skin
In vitro assay
Wound Healing Effect

Migration assay
Cell strain Human epidermal Keratinocyte (C-001-5C, Cascade)
Detection method Ibidi GmbH kit (Germany)
BIO-Placenta BIO-Placenta
Control
0.01% 0.1%
0hr
14hrs
19hrs
The migration ability of Keratinocyte is improved by using BIO-Placenta

In vitro assay
Stimulation of Cell Growth

MTT assay
Cell strain Human Derma Fibroblast neonatal (HDFn, LONZA)
Detection method MTT assay (Sigma, US)
120 Commercial Human Placenta Product

BIO-Placenta
115
110
Growth rate(%)
105
100
95
90
85
80
0 0.78 1.56 3.13 6.25 12.50
Concentration(mg/mL)
BIO-Placenta is more effective than commercial human placenta product

in stimulation of cell growth
In vitro assay
Biosynthesis of Collagen
Detection method Western blot
BIO-Placenta
0.02 0.3 0.47
Pro-collagen type 1
BIO-Placenta stimulates collagen biosynthesis of fibroblasts

In vivo assay
SUMMARY OF THE CLINICAL STUDY

12 female aged 43 to 50
Subjects of experiment
(average age : 45.75)
Constant temperature and humidity room

Place of experiment
(22±1℃, 45±5%)
Experimental period 4 weeks
Test area Facial region including crow’s feet
Test sample BIO-Placenta Cream (BIO-Placenta : 3%)
Test material application Spread suitable quantity on facial region including crow’s feet
1. Evaluating wrinkle improvement through PRIMOS Lite and Robo Skin

Analyzer
2. Evaluating skin elasticity improvement through DermaLab elasticity
Evaluation method probe and Robo Skin Analyzer
3. Evaluating moisturizing improvement through DermaLab moisture probe
4. Visual evaluating according to global photodamage
5. Evaluation of abnormal response
6. Survey
Statistic analysis methods SPSS 17.0 for Windows
Test institute Korea Institute for Skin and Clinical Sciences

In vivo assay
Anti-wrinkle
Test area Crow’s feet
Detection method PRIMOS Lite 3D Face and Skin ScannerAnalyzing System, GFMesstechnik
Before After
Reduces wrinkles by 13% in 2 weeks and 16% in 4 weeks on crow’s feet

In vivo assay
Anti-wrinkle
Detection method PRIMOS Lite 3D Face and Skin ScannerAnalyzing System, GFMesstechnik
28 20
27
Improvement rate(%)
26 15
Average of Ra
25
24 10
23
22 5
21
20 0
Before 2 weeks 4 weeks Before 2 weeks 4 weeks
Ra : Average of all heights and depths to the reference plane

In vivo assay
Anti-wrinkle
Test area Below eyes
Detection method Robo Skin Analyzer CS50, Inforward, Inc.
Before
Wrinkles: 10 6
After
Wrinkles: 2 1
Reduces wrinkles by 38% in 2 weeks and 46% in 4 weeks on below eyes

In vivo assay
Anti-wrinkle
Test area Below eyes
6 60
Improvement rate(%)
Number of wrinkles
5 50
4 40
Left 3 30
2 20
1 10
0 0
6 60
Improvement rate(%)
Number of wrinkles
5 50
4 40
Right 3 30
2 20
1 10
0 0
Reduces wrinkles by 38% in 2 weeks and 46% in 4 weeks on below eyes

In vivo assay
Anti-wrinkle
Before
Total length of wrinkle: 47mm
After
Total length of wrinkle: 21mm

In vivo assay
Anti-wrinkle
80 25
Average changes in Ra values(μs)
70
20
Improvement rate(%)
60
50 15
40
30 10
20
5
10
0 0

In vivo assay
Skin elasticity
Test area Facial area
Detection method DermaLab elasticity probe, Cortex Technology, Inc.
12 50
10 40
Improvement rate(%)
Skin elasticity(Mpa)
8
30
6
20
4
2 10
0 0
Mpa=Mega pascal, N=12
Skin elasticity has been improved by 32% in 2 weeks and 47% in 4 weeks
In vivo assay
Moisturizing
Detection method DermaLab moisture probe, Cortex Technology, Inc.
700 90
Moisture Average changes in skin(μs)
600 80
70
Improvement rate(%)
500
60
400 50
300 40
30
200
20
100 10
0 0
Before Just applied 2 weeks 4 weeks Before Just applied 2 weeks 4 weeks
μs= micro siemens, μs=1/106Ω, N=12
Skin Moisturizing has been improved by 83% just after applying,

50% in 2 weeks and 64% in 4 weeks
Function of Growth factors
EGF
Epidermal Growth Factor
‣ Mitogenic effect of cells

‣ Wound healing effect by
epithelialization
‣ Growth, differentiation and
migration of cells
‣ Wrinkle care
‣ Activation of hyaluronan
synthase 2 in human
epidermal keratinocyte
Ref. Chemistry and biology of hyaluronan, Hari G. Garg,
Charles A. Hales, Elsevier, 2004
IGF-1
Insuline-like Growth Factor-1
• Effect of IGF-1 restoration on Blank EGF+IGF-1
phospholipid profile in skin

• Angiogenic effect
• Induction of synergic effect of EGF
on wound healing and growth
stimulation of fibroblast cell IGF-1 EGF
• IGF-1 increases the synthesis of
hyaluronan and chondroitin sulfate
proteoglycan by tissue fibroblast
Fig. Effect of growth factor signalling on
wound epithelialization.
Ref; Journal of Cell Science 116, 3227-3248

FGF
Fibroblast Growth Factor
• Growth and mitogenesis

stimulation of fibroblast cells
• Stimulation of skin re-generation
• Stimulation of Collagen and Elastin
synthesis in fibroblast cells
• Wound healing effect
• basic FGF(FGF-2) activates
hyaluronan synthesis with IGF-1 in
human epidermal fibroblast
VEGF
Vascular Endothelial Growth Factor
Natural
aging
• Management of capillary vessels

• Induction of nutrient supplements
to fibroblast cells
• Growth and migration of cells
• Wound healing effect
Analysis of Growth factors
Biological Activity
Control BIO-Placenta Control EGF Control IGF-1
Control a-FGF Control bFGF Control VEGF

Analysis of Growth factors
Biological Activity
BIO-Placenta EGF IGF-1
240 240 240
180 180 180
Growth rate (%)
Growth rate (%)

Growth rate (%)
120 120 120
60 60 60
0 0 0
0.000 0.003 0.015 0.06 0.25 1 0.000 0.005 0.05 0.5 5 0.000 0.005 0.05 0.5 5
10
5
1
05
5
1
4
5
10
2
01
06
25
01
1
0
05
00
5
01
8
5
1
00
0.
03
0.
00
1
1
00
00
00
00
0.
00
0.
00
0.
0.
00
0.
0.
0.
0.
0.
0.
0.0
0.
0.0
0.
0.
0.
0.
0.
0.
0.
0.
0.
BIO-Placenta (%) EGF (ng/ml) IGF-1 (ng/ml)
acidic FGF basic FGF VEGF
240 240 240
180 180 180

Growth rate (%)
Growth rate (%)
Growth rate (%)

120 120 120
60 60 60
0 0 0
0.000 0.005 0.05 0.5 5 0.000 0.005 0.05 0.5 5 0.000 0.005 0.05 0.5 5
10
10
01
5
1
5
01
5
10
1
5
1
5
1
5
5
05
00
00
01
5
05
00
1
0.
0.
0.
0.
0
0
0.
0.
00
0
0
0.
0.
0.
0
0.0
0.0
0
0.
0.
0.
0
0.0
0.
0.
0.
0.
0.
0.
aFGF (ng/ml) bFGF (ng/ml) VEGF (ng/ml)

Function of Essential factors
Vitamin
B9-Vitapol
• The formation of B9-Vitapol is made through structural changes of folic acid into the
natural form of polyglutamate.
• It is produced to improve the negative characteristics (pH sensitivity and low solubility)
of folic acid through Korean traditional fermentation technology.
γ-polyglutamate Vitamin B9(Folic acid)
New fermentation technology based on Korean traditional fermentation
B9-Vitapol
Function of Essential factors
Amino acid
Hydracell Q
Acetylated Glutamine, which is reformulated to improve the stability.
Glutamine Improve
Stability Hydracell Q
O O
H
H2 N N CH2
OH HO
The amines in glutamine
is very unstable
O
Acetylated amino
group
O H2 N O
H2 N
Summary
Definition
Contains five different growth factors (EGF, IGF-1, acidic FGF, basic FGF and VEGF), amino acid and vitamin
These growth factors are safely synthesized
INCI
Water, Lecithin, Acetyl glutamine, sh-Oligopeptide-1, sh-Oligopeptide-2, sh-Polypeptide-1, sh-
Polypeptide-9, sh-Polypeptide-11, Bacillus/soybean/folic acid ferment extract, Sodium hyaluronate,
Caprylyl glycol, Butylene glycol, 1,2-Hexanediol
Physical characteristics
Liquid form of liposome
Benefits & Uses

Anti-wrinkle
Skin rejuvenation
Elasticity enhancement
Moisturizing
Cell vitalization
Application
Skin, Body and Hair care
Recommended dosages
3.0%
Cell hydration, Anti-stress
Hydracell Q/QS/QQ
Water your skin cells like drinking aqua
Acetylated Glutamine, reformulated to improve the stability

Dry Skin
Dehydrated
Dry skin
Wate
Pleas r
e!!!
New Solution
Dehydration H2 O Rehydration
H2 O H2 O H2 O
Dry Skin Hydrated Skin
Hydracell Q
Function of Glutamine
re s s
St
H2O
H2O
H2O Gln
H2O
H2O
H2O
Transport
H2O Channel
H2O Gln
H2O Correlation between
H2O Glutamine and ATP
120
Energy 100
Metabolism
80
Swelling
% ATP
60
40
Immune 20
0
Function Anti-stress 0.05 0.2 0.6 2
Effect mM glutamine
Application to Cosmetics
Improve
Glutamine Stability Hydracell Q
O O
H
H2 N N CH2
OH HO
The amines in glutamine is
very unstable
O
Acetylated amino group
O H2 N O
H2 N
Reference: Snowden, M.K. et al, Journal of Food Science 67: 384-389(2002)

Marti, M.B. et al, Journal of Agricultural Food Chemistry, 48(12), 6003-6010(2000)
Improved stability has made it available for use in liquid cosmetics

Advantages of Hydracell Q
Transpathway Mechanism
Anabolic Pathway
Amino acids
Catabolic Pathway Glucose
Swelling
Proteins
Glycogen
Hydracell Q changes catabolic pathway to anabolic pathway,

which leads to produce proteins and polymers such as collagen and glycogen
In vitro assay
Biosynthesis of Collagen
Cell strain Human Dermal Fibroblast Neonatal(HDFN, LONZA)
Detection method Western blot
/mL
/mL
/mL
mg
mg
mg
10 -3
10 -2
10 -1
ll Q
ll Q
ll Q
e
e
rac
rac
rac
ol
ntr
d
Co
Hy
Hy
Hy
Collagen biosynthesis is activated by changing catabolic pathway to anabolic pathway

In vitro assay
Anti-stress Effect
Cell strain Human Dermal Fibroblast neonatal(HDFn, LONZA)
Detection method NR assay & Trypan Blue stainning
0.4mM H2O2
A B C Hydracell Q
Hydracell Q+0.4mM H2O2
120.00
110.00
100.00
Cell Viability(%)
90.00
80.00
0.4mM 0.125% 70.00
H2O2 Hydracell Q 0.4mM
H2O2 60.00
+
0.125% 50.00
Hydracell Q 0.008 0.016 0.032 0.063 0.125
Hydracell Q Concentration(%)
Hydracell Q prevents damages that are caused by oxidative stress

through cell swelling process
Hyaluronic Acid VS Hydracell Q
Hyaluronic Acid
(Low molecular weight)
10,000 Da.
Hydracell Q
(Single particle)
146 Da.
Hyaluronic Acid
(High molecular weight)
1,000,000 Da.
Hydracell Q HA Low molecular weight HA High molecular weight

Hydracell QQ
Synergic Effect
Hydracell Q + HA
Hydracell Q
Hydracell QQ
Hyaluronic Acid Locking
Moisture
Hydracell Q Supplying
Double
Action
Hyaluronic Acid
In vivo assay
SUMMARY OF THE CLINICAL STUDY

12 male and female aged 20 to 31
Subjects of experiment
(average age : 23.92)
constant temperature and humidity room

Place of experiment
(22±1℃, 45±5%)
Experimental period 24 hours
Test area left forearm and facial area
Test sample Hydracell Q Cream (Hydracell Q : 2%)
1. Subjects washed their forearms and face with the designated cleanser.
2. Two grams of Hydracell Q cream was evenly applied on the facial area.
Test material application 3. Fifty micrograms of Hydracell Q cream was evenly applied to the left
forearm test site (3.0cm × 3.0 cm) using a disposable syringe (Sungshim
Medical Co., Ltd., Korea). A latex finger cot was used for absorption.
1. Moisture was evaluated using a DermaLab moisture probe

2. TEWL evaluated by the DermaLab TEWL probe
3. Skin texture evaluation by PRIMOS Lite
Evaluation method
4. Evaluation of epidermal barrier quality by the keratin sticker
5. Evaluation of abnormal response
6. Survey
Statistic analysis methods SPSS 17.0 for Windows
Test institute Korea Institute for Skin and Clinical Sciences

In vivo assay
24-hour moisturizing
Applied area
Average of moisture content(μs)

Non-applied area
800
600
400
Detection DermaLab moisture probe,
method Cortex Technology, Inc.
200
0
0 0.5 4 8 12 24
μs= micro siemens, μs=1/106Ω, N=12
Average of TEWL(g/m2/hr)
6
5
Test area Left forearm 4
3
Detection DermaLab moisture probe,
method Cortex Technology, Inc. 2
1
0
0 0.5 4 8 12 24
In vivo assay
Skin Texture
Test area Left forehead
Detection method PRIMOS Lite 3D Face and Skin Scanner Analyzing System, GFMesstechnik
21 12
Average changes in Ra values(μs)
10
Improvement rate(%)
20
8
19 6
4
18
2
17 0
Before After Before After
Ra : Average of all heights and depths to the reference plane
Skin texture has been improved 11% after treatment

In vivo assay
Epidermal Barrier Quality

Test area Left Cheek
Detection method Keratin sticker(KISCS), Digital light microscope(Advanced Microscopy Group)
Before After
Epidermal barrier quality has been improved 40% after treatment

Summary
Hydracell Q Hydracell QS Hydracell QQ

Combination of Sodium hyaluronate
Acetylated Glutamine which is and 5% of Hydracell Q,
10% water solution version of
Definition reformulated which is reformulated to improve
Hydracell Q
to improve the stability the stability and to help higher
moisturizing
Water, Dipotassium phosphate, Polyglycerin-10, Water, Dipotassium
Acetyl glutamine, Butylene glycol, phosphate, Acetyl glutamine,
INCI Acetyl glutamine
1,2-Hexanediol, Sodium Butylene glycol, 1,2-Hexanediol,
carboxymethyl cellulose Sodium hyaluronate
Physical
Powder Transparent liquid Transparent liquid
Form
Recommended
1.0~2.0% 3.0~5.0% 3.0~5.0%
Dosage
Benefits & Uses
Anti-stress
Biosynthesis of collagen
Cell activation
Cell growth stimulation
Application
Skin, Body, Sun and Hair care

Innovative formulation of Ceramide
Ceramide3B Series
Now, You can feel the real ceramide
By using clearly solubilized ceramide, it helps to arrange of the lipids in stratum corneum
This is the key to form the skin barrier

Skin Barrier
Source: Human skin-identical Ceramides, Evonik Industries, P.4 (2007)
The protection of the skin is provided primarily by the outermost layer of the epidermis
– the stratum corneum
Composition of Skin Lipids
Free fatty acids

(25%)
Stratum
corneum
Skin Barrier
Ceramide Cholesteryl
(40~50%)
Differentiation process
Stratum Sulphate(5%)
granulosum
Stratum
spinosum
Cholesterol
(30%)
Stratum
basale
Epidermis Skin barrier lipid consists

Source: Human skin-identical Ceramides, Evonik Industries, P.7 (2007) 40~50% of Ceramide
The major lipid classes in the stratum corneum are Ceramides, cholesterol and fatty acids
Aging & Ceramide level
Age Ceramide level
21~30 years 100%
Hands 31~40 years 78%
41~50 years 63%
21~30 years 100%
Face 31~40 years 62%
41~50 years 37%
Source: A.V. Rawlings et al. in Biocosmetics-Skin aging, IFSCC, vol. I, 31-45 (1993).
Skin ceramide level decreases rapidly with age

Aging & Ceramide level
"Quantitative and Qualitative Analysis of Ceramides in the Stratum Corneum of Normal

and Dry Skin " M. Chopart et al., L’Oréal Advanced Research Laboratories (FR).
Stratum Corneum III, Poster (2001).
Test design
Comparison of Ceramide content and profile of normal (39 volunteers)
and dry skin conditions (25 volunteers).
Source: Human skin-identical Ceramides, Evonik Industries, P.18 (2007)
In dry skin, the total ceramide content is decreasing

In dry skin, there is a deficiency in phytosphingosine based ceramides
Phase behavior of Ceramide
Ceramide IIIB
As ceramide crystalizes easily, it is usually unstable in liquid phase

New Solution for Ceramide
Emulsion Liposome
type type Ceramide3B ST
Water
Lecithin
Crystalized
Ceramide
Ceramide has been using in the form of emulsion and liposome type for maintaining its stability in the
formulation, but Ceramide3B ST improves skin barrier function and skin feeling of ceramide through
solubilization
New BIO-surfactant, MEL
MEL
(Mannosylerythritol Lipid)
Hydrophobic Asymmetric structure
Combines with Ceramide Prohibit Ceramides from

crystalization
Key factor for solubilizing the ceramide, MEL

MELts Ceramide
Lamellar-to-amorphous
Ceramide phase transition
MEL
MEL is inserted in the lamella structure of ceramide and inhibits crystallization of ceramide
In conclusion, MEL helps to solubilize ceramide
Easy Manufacturing
Normal Solubilization
Solubilization with DAMY’s technology
High temperature
(150℃)
Low temperature
(80℃)
Not transparent Transparent

(Liposome type) (Smaller particle)
Solubilization process doesn’t need high temperature

Morphology Analysis
Ceramide3B ST solution
(Containing 20% ceramide)
According to SEM, the morphology of Ceramide3B ST solution is in stable in sphere form

Stability test
Case of Ceramide3B ST solution is stable according to measuring zeta potential

Long term Storage Stability
After 1month storage After 1month storage

Ceramide3B ST solution Ceramide 20% solution
(Containing 20% ceramide)
Ceramide3B ST is still in the form of liquid crystal after 1 month storage

Application Tip
Toner or Cream?
m
ea
To
n
Cr
er
DP DPS
Rich cream containing Transparent liquid formulation
high concentration of Ceramide through containing up to 0.05% of Ceramide
easy solubilizing process
Liquid formulation containing
0.5% of Ceramide with stability
Various options to apply Ceramide in formualtion with wide range of different concentrations
Application
Emulsion Liposome Ceramide3B

type type VS
Series
Mist
Toner Toner
(Two layer/Milky)
Essence
Essence
Emulsion Emulsion
Water
Cream Cream
Lecithin
Make-up Make-up
Summary
Ceramide3B ST Ceramide3B DP Ceramide3B DPS(0.5%)
Water soluble ceramide3 Water and oil soluble ceramide3 in high

Definition 5% of Ceramide3B DP in solution
in high transparency transparency
Ceramide3B
Content 20% 10% 0.5%
Water, Butylene glycol, 1,2-Hexanediol,

Mannosylerythritol lipid, Ceramide 3, Pseudozyma/epicola/soybean flour/olive fruit oil
Pseudozyma/epicola/soybean flour/olive fruit oil
Dipropylene glycol, Butylene glycol, 1,2- ferment filtrate, Mannosylerythritol lipid,
ferment filtrate, Mannosylerythritol lipid,
INCI Hexanediol, Polysorbate 20, Glycerin, Stearoyl Ceteareth-20, PEG 40-Hydrogenated castor oil,
Ceteareth-20, PEG 60-Hydrogenated castor oil,
glutamic acid, Lauroyl glutamic acid, Ceramide 3, Polysorbate 20, Cetearyl alcohol,
PEG 40-Hydrogenated castor oil, Ceramide 3,
Polysorbate 80, Cocoyl glutamic acid Propanediol
Polysorbate 20, Cetearyl alcohol, Propanediol
Physical
form Paste Paste Colorless transparent liquid
Recommended
dosage 2.0~3.0% 2.0~3.0% 2.0~3.0%
Benefits & Uses
Skin barrier enhancement, Anti-aging, Skin rejuvenation

Elasticity enhancement, Moisturizing
Application
Skin, Body, Sun and Hair care

Solve the butter easier and
feel it deeper KR Patent / PCT Pending
Shea Butter ST Unique texture of liquid and butter

through 10% solution of Shea butter
Water solubilized shea butter created by fermentaton technology delivers unique texture combination
of water and butter

Concept
Shea Butter x Water

= Shea Butter ST
Water soluble Shea Butter

Advantages
n er
To
Essence Extension of application
inspires marketer
Water-solublized a whole shea buttter

is different from shea butter extract
Improvement of stability
lessens the formulator’s distress
Water-absorption & butter-covering

delivers real moisturizing to users
Oi
l-f
Minglement of richness and freshness
re provides pleasant texture to users
e
Advantages
Water-solubilized
a whole shea butter
Shea Butter ST is different from shea butter extract.
Shea Butter ST is different from PEG-shea butter.
It is solubilized in water by bio-surfactant called ‘MEL’.
Mist
er
Extension of application
n
r
To
te
But
Balm
Complete all the skin care lines
Crea m
Emu with Shea Butter ST.
lsio
n
M senc
as
Es
k
Sham
sh
ee
t
e
poo
Advantages
Water-absorption & butter-covering

Co-existance of water and butter
delivers real moisturizing to users.
Because moisturizing is completed
with ‘water supplying’ and ‘water locking’.
Minglement of richness Oi
l-f
and freshness re
e
t e r !
Shea butter ST provides pleasant texture
r o - but
to users. Feel what hydro-butter is.
Hy d
Advantages
Improvement of stability
Shea Butter ST lessens the formulator’s distress.
Bio-surfactant firmly holds water and butter in water
phase.
Water Butter
Solubilization
Technology
MEL (Mannosylerithritol lipid)

is a bio-surfactant of glycolipid-type
from yeast fermentation
Butter Water soluble butter
MEL
Water Butter
Solubilization
Insoluble Material New solution Soluble Material

Stability test
Stability
Zeta Potential Distribution
Detection method: Real-time PCR
Apparent Zeta Potential (mV)
Zeta Potential (mV) : -39.7 Temperature (oC) : 25.0

Zeta Deviation (mV) : 13.6 Count Rate (kcps) : 184.3
Conductivity (mS/cm) : 0.0318 Cell Description : Clear disposble zeta cell
Case of Shea Butter ST solution is stable according to measuring zeta potential

Stability test
Stability
Temperature
Shea Butter ST(Shea butter 0.5%) 3 weeks later
-20 C -5 C
o o
5Co
25 C 40 C Cycle RT
o o
Shea Butter ST shows stable condition under wide range of temperature

Skin barrier
External Dry
environment
Chemical
based materials
Excessive
wash
UV
rays Pollution
Factor
Shea Butter
Internal
Atopic Xeroderma Natural
Factor skin Oxidation
About Shea Butter
Shea Butter
Fatty Acid Etc.
Cinnamic acid esters Natural UV protection material
Oleic acid Stearic acid
(20~50%) Vitamin E Anti-oxidative effect, Anti-aging
(40~60%)
Catechins
Phytosterols Conditioning
Shea Butter
Arachidic acid Linoleic acid

(<1%) (3~11%)
Linolenic acid
(<1%) Palmitic acid
(2~9%)
Source: http://en.wikipedia.org/wiki/Shea_butter
1. Wiesman, Z, S. Marans, G..Bianchi, and J Bisgaard. 2003. Chemical analysis of fruits of Vitellaria paradoxa. In Teklehaimanot, Z (Ed) Improved Management of
Agrofrestry Parkland Systems in Sub-Saharan Africa, Final Report. School of Agricultural and Forest Sciences, University of Wales: Bangor, U.K.; pp131-139.
2. Badifu, G.I.O. 1989. Lipid composition of Nigerian Butyrospermum paradoxum kernel. J Food Compos Anal 2:238-244.
The major lipid classes in the stratum corneum are Ceramides, cholesterol and fatty acids
Summary
Definition
Shea Butter ST is the complex product which can solubilize Shea Butter clearly in water
INCI
Water, Glycerin, Shea Butter, Mannosylerythritol Lipid, PEG-40 Hydrogenated Caster Oil,
Cetearyl Alcohol, Ceteth-20 Phosphate, Dicetyl Phosphate,
Cermaide 3, Phenoxyethanol, Arginine
Physical characteristics
Transparent liquid
Benefits & Uses

Skin barrier enhancement
Moisturizing
Improvement of irritated skin
Application
Skin, Body and Hair care
Recommended dosages
3.0 ~ 5.0%
THANK YOU

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Anti-pollution

19th. JUNE. 2015

World Carbon Dioxide Emissions by Country

Health Eeffects of pollution

Air pollution Damages

Ozone Oxidative stress

Soot aerosol Oxidative stress

Nitrogen oxide DNA damages

UV radiation Oxidative stress

SO2 is colorless gas with an irritating pungent odor

DNA damage DNA damage

The first target of Ozone in the skin

PAHs induce melancyte proliferation and thereby skin

Soot aerosol Pigmentation & aging

Cigarette smoke is a highly complex aerosol composed of thousands of chemical substances,

NO2 and NO can destroy

Air pollution Damages

Ozone F. Shiunko Oxidative stress

Airborne particles F. Shiunko Pigmentation

Soot aerosol BIO-Placenta Oxidative stress

Recommendation from DAMY

Skin soothing VB9-plus

COPYRIGHT © DAMY CHEMICAL CO., LTD. ALL RIGHTS RESERVED.

Choosing the best oil Creating new category of oil

Pseudozyma sp. SY-16[KCTC 8950P]

• Odorless in aerobic condition

• KRIBB have researched to develop organisms that can

the oil leakage in 2009

The patent was registered through cooperation with Damy and

MEL Pseudozyma sp.

MEL concentration in olive oil (%,v/v)

Detection method 2D-TLC Analysis

2nd Solvent Development Olive oil Fermentoil Olive

1st Solvent Development

Comparing to the result of TLC Analysis,

Detection method Metrohm Titrato

Olive oil Fermentoil Olive

Olive oil Fermentoil Olive

Comparing to the result before fermentation,

Detection method HPLC Analysis

Linoleic acid Palmitic acid Oleic acid Stearic acid

Detection method 2D-TLC Analysis

2nd Solvent Development Argan oil Fermentoil Argan

1st Solvent Development

Comparing to the result of TLC Analysis,

Detection method Metrohm Titrato

Argan oil Fermentoil Argan

Argan oil Fermentoil Argan

Comparing to the result before fermentation,

Detection method HPLC Analysis

Linoleic acid Palmitic acid Oleic acid Stearic acid

Detection method 2D-TLC Analysis

Green tea seed oil Fermentoil GTS

1st Solvent Development

Comparing to the result of TLC Analysis,

Detection method Metrohm Titrato

Green tea seed oil Fermentoil GTS

Green tea seed oil Fermentoil GTS

Comparing to the result before fermentation,

Detection method HPLC Analysis

Green tea seed oil

Linoleic acid Palmitic acid Oleic acid Stearic acid

ㄹ Pseudozyma Oil Feeding(0hr) Fermention(60hr) Fermentation(120hr)