BIOPHYSICAL CHEMISTRY

CHEM F323

Welcome
 General

Dear Students

• If I am disconnected from the scheduled class during 9.00

– 9.50 am, please don’t leave the class because that might

be due the Network problem.

• I shall reconnect again and continue the lecture.

• If you have any doubt, just contact me at 9929832205.

 Topics
• Amino acids and primary structure of proteins
• Ionic properties of amino acids Isoelectric point Ionization equilibrium in protein
Environmental preference of amino acids Classifying the proteins based on polarity of
primary structure
• Intermolecular interactions
• Charge-charge Ion – dipole Dipole – dipole Lennard Jones interaction
Hydrogen bonding hydrophobic interaction
• Higher level structure of protein
• Secondary Tertiary Quaternary
• Nucleic acids
• bonding interaction secondary and tertiary structure Ionization
equilibrium
• Lipids, bilayers and monolayers
• Forces and factors governing the aggregation in bilayer and monolayer . Protein-lipid
interaction Membrane equilibria Donnan effect
• Protein conformation and folding
• Experimental techniques
• X-ray crystallography Light scattering Dielectric spectroscopy
• Molecular Recognisation
 Topics
• Amino acids and primary structure of proteins
• Ionic properties of amino acids Isoelectric point Ionization equilibrium in protein
Environmental preference of amino acids Classifying the proteins based on polarity of
primary structure
 Topics
• Amino acids and primary structure of proteins
• Ionic properties of amino acids Isoelectric point Ionization equilibrium in protein
Environmental preference of amino acids Classifying the proteins based on polarity of
primary structure
 Topics
• Amino acids and primary structure of proteins
• Ionic properties of amino acids Isoelectric point Ionization equilibrium in protein
Environmental preference of amino acids Classifying the proteins based on polarity of
primary structure
 Topics
• Amino acids and primary structure of proteins
• Ionic properties of amino acids Isoelectric point Ionization equilibrium in protein
Environmental preference of amino acids Classifying the proteins based on polarity of
primary structure
 Topics
• Amino acids and primary structure of proteins
• Ionic properties of amino acids Isoelectric point Ionization equilibrium in protein
Environmental preference of amino acids Classifying the proteins based on polarity of
primary structure
• Intermolecular interactions
• Charge-charge Ion – dipole Dipole – dipole Lennard Jones interaction
Hydrogen bonding hydrophobic interaction
• Higher level structure of protein
• Secondary Tertiary Quaternary
• Nucleic acids
• bonding interaction secondary and tertiary structure Ionization
equilibrium
• Lipids, bilayers and monolayers
• Forces and factors governing the aggregation in bilayer and monolayer . Protein-lipid
interaction Membrane equilibria Donnan effect
• Protein conformation and folding
• Experimental techniques
• X-ray crystallography Light scattering Dielectric spectroscopy
• Molecular Recognisation
 BIOPHYSICAL CHEMISTRY

Component Duration Weightage Date & Time

(%)

Test 1 30 Min. 15 Sep 10 – Sep 20 (During

scheduled class hour)

Test 2 30 Min. 15 Oct 09 – Oct 20 (During

scheduled class hour)

Test 3 30 Min. 10 Nov 10 – Nov 20 (During

scheduled class hour)

Quizzes/ 30 Continuous
assignments

Comprehensive 120 Min. 30 As announced in the Timetable

Examination (02/12 FN)
 BIOPHYSICAL CHEMISTRY

• Exploring biological molecules by laws of physics

• Systems are chemical
• Tools: EXPT THEORY SIMULATION
• Physics, Chemistry, Biology and Mathematics
• Purpose is to understand the living things
• Complex systems – multi-component and heterogeneous –
difficult than simple systems (single component)
• The simpler building blocks interplay – they are understood
• Understanding the complex systems in molecular detail is
difficult
 An overview of systems: Basic unit is chemical but have
function related to life

Main classes:
1. Proteins
2. Nucleic acids
3. Lipids Glycine and Hemoglobin
4. Carbohydrates

Lipids and Membrane Bases and DNA

Lipids