A. Translucent Spot Test 2.

Heat the samples slowly and note the odor

produced. Glycerine, olive and coconut oil
1.In a small filter paper, drop a lipid sample. Do
produced a pungent odor while lecithin
this to the four samples separately. Allow the
produces an irritating smell
spots to dry
Glycerine – pungent odor
2. After drying, hold the filter paper against a
light and see any translucent spot. Record your Olive oil – pungent odor
observation.
Coconut oil - pungent odor
Glycerine +
Lecithin – irritating smell
Olive oil +
D. Solubility Test
Coconut oil +
1. In five different test tubes add 1 ml each of
Lecithin + water, dichloromethane, cyclohexane, 1M HCl
and 1M NaOH.

2. Add to each test tube three drops of each of

B. Unsaturation Test
the lipid samples and mix thoroughly. Make
1. Place 3 ml of ether in five separate test tubes. sure you place strictly three drops of sample on
Label the fifth test tube as negative control each solvent as solubility is affected by amount.

2. Add the lipid sample in each test tube while 3. Mix thoroughly and observe if sample is
add none to the control. Mix thoroughly. The thoroughly dissolved. See separation of layers
sequence of the sample starts from glycerine, to detect miscibility. Record your observations
coconut oil, olive oil, lecithin, and negative
Glycerine Oil
control
Water - insoluble
3. To each test tube add 3 drops of I2 in KI.
Shake again and note the changes in color. The Dichloromethane - soluble
sequence of the sample starts from glycerine,
Cyclohexane - soluble
coconut oil, olive oil, lecithin, and negative
control Dilute HCl - soluble
Glycerine – Red Dilute NaOH - soluble
coconut oil – Red Orange Coconut Oil
olive oil - Pink Water - insoluble
lecithin - Pink Dichloromethane - soluble
negative control – Pink Cyclohexane - soluble
C. Acrolein Test Dilute HCl - soluble
1. In a clean crucible, place 0.5 gram of Dilute NaOH - insoluble
potassium bisulfate and one to two drops of
lipid sample. Then cover. Olive Oil
 Water - insoluble

Dichloromethane - soluble

Cyclohexane - soluble

Dilute HCl - soluble

Dilute NaOH - insoluble

Lecithin

Water - insoluble

Dichloromethane - insoluble

Cyclohexane - insoluble

Dilute HCl - insoluble

Dilute NaOH - insouble