BioE.

335
Homework 5

Problem 1. Calculate lA , the heat of adsorption for lysozyme adsorbing onto glass from the
data in the two isotherms below. Data were collected at two different temperatures, 25 oC and 30
o
C. Assume ideal Langmiur adsorption applies.
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There are a couple of ways to work this; a sneaky way that only uses a part of all the data and the
second which uses all.
Method 1. Pick a QA common to both isotherms as in the green arrows above; thus yielding two
different bulk protein concentrations.

Method 2. Uses all

the data for both
curves.

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 BioE. 335

Problem 2. Determine Km and Vmax from the rates of an enzyme-catalyzed reaction in table below.
Assume Menton-Michaelis kinetics prevails.
S,
(mol/liter) 4.1E-03 9.5E-04 5.2E-04 1.0E-04 4.9E-05 1.1E-05 5.1E-06
V
(mol/liter- 1.429E-04 1.33E-04 1.25E-04 1.12E-04 0.99E-04 0.67E-04 0.43E-04
min)

1/S
243.0 1052.6 1923.0 10000 20408.0 90909.0 196078.4
(lit/mol)
1/V (lit-
7,000. 7500.0 8000.0 8950.0 10100. 14925.4 23255.8
min/mol)
If V = Vmax S /(Km + S), then plotting 1/V vs. 1/S yields straight line with slope =
Km/Vmax and intercept = 1/Vmax. Intercept = 7751. Slope = 0.0794.

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 BioE. 335
25000.00
y = 0.0794x + 7751.7
1/V (lit-
R² = 0.9932
min/mol)
20000.00

15000.00

10000.00

5000.00

0.00
0.00 50000.00 100000.00 150000.00 200000.00 250000.00
1/S, (lit/mol)

Vmax = 1.290E-04 mol/min Km = 1.025E-05 mol/liter

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