Potato dextrose agar

Potato dextrose agar (BAM Media M127[1]) and potato dextrose

broth are common microbiological growth media made from potato
infusion, and dextrose. Potato dextrose agar (abbreviated "PDA") is the
most widely used medium for growing fungi and bacteria.[2]

Typical composition
value ingredients & conditions Aspergillus sp. growing in potato
dextrose agar
1000ml water
potatoes
4g (from 200g infused potato)
(sliced washed unpeeled)

20g dextrose
20g agar powder
5.6±0.2 final pH
25°C temperature

Potato infusion can be made by boiling 200 grams (7.1 oz) of sliced (washed but unpeeled) potatoes in ~ 1
litre (0.22 imp gal; 0.26 US gal) distilled water for 30 minutes and then decanting or straining the broth
through cheesecloth. Distilled water is added such that the total volume of the suspension is 1 litre
(0.22 imp gal; 0.26 US gal). 20 grams (0.71 oz) dextrose and 20 grams (0.71 oz) agar powder is then added
and the medium is sterilized by autoclaving at 15 pounds per square inch (100 kPa) for 15 minutes.[1]

A similar growth medium, Potato dextrose broth (abbreviated "PDB") is formulated identically to PDA,
omitting the agar.[3] Common organisms that can be cultured on PDB are yeasts such as Candida albicans and
Saccharomyces cerevisiae and molds such as Aspergillus niger.[3]

References
1. BAM Media M127: Potato Dextrose Agar (https://www.fda.gov/food/foodscienceresearch/labora
torymethods/ucm063519.htm) from the U.S. Food and Drug Administration
2. Harold Eddleman, Ph. D (February 1998). "Making Bacteria Media from Potato" (http://www.dis
knet.com/indiana_biolab/b029.htm). Indiana Biolab. disknet.com. Retrieved March 4, 2011.
3. "Potato Dextrose Broth" (https://web.archive.org/web/20060516172216/http://www.emdchemic
als.com/analytics/Micro_Manual/TEDISdata/prods/1_00510_0500.html). Merck KGaA.
Archived from the original (http://www.emdchemicals.com/analytics/Micro_Manual/TEDISdata/
prods/1_00510_0500.html) on May 16, 2006. Retrieved May 29, 2005.

Further reading
Atlas, R.M.: Handbook of Microbiological Media, second edition. Lawrence C. Parks (1997)
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