Question Answers PICTURE/VISUAL PRESENTATION

1) What is  Culture media,

Culture Media? also known as
growth media,
 
are complex
mixtures of
nutrients and
other
substances
that help
bacteria and
 
fungi grow. In
applications
such as
hygiene
testing, culture
media are used
for quality
control tests of
nonsterile raw
materials and
finished goods,
as well as
microbial
contamination
tests.
2) Types of  
Culture Media:  
  1. Liquid media
 a) According to 2. Solid media
Consistency
     (Give only 2)

 
 b) According to  
Chemical  1. Basal media
     Composition 2. Enriched
    (Give only 2 ) media

 
   

 c) Common
Media in 1. Transport
routine  media
    Use 2. Storage
media
    (Give only 2 )

   
3) Components  
of Culture  1. Water
    Media
 

 
  2. Electrolytes

 
  3. Peptone

 
  4. Blood

 
  5. Meat extract,
yeast extract

 
4) Preparation Procedure:
of
Culture Media
Suspend 28 g of
  nutrient agar
powder in 1 litre
     a. Nutrient of distilled water.
Agar Bring to the boil
to dissolve
completely.
Dispense as
required and  
sterilize.

     b. Nutrient  Add 13 g of

Broth nutrient broth
powder to 1 litre
of distilled water.
Mix well.
Dispense as
required and
sterilize.

 
     c. Malt  Suspend 18g
Extract Sugar agar powder in 1
litre of distilled
water. Bring to
the boil to
dissolve
completely. Add
15g malt extract
per litre. Mix
well. Dispense as
required and  
sterilize.

     d. Mannitol  Suspend 10 g

Yeast Extract  agar in 1 litre of
distilled water.
         Sugar Heat to dissolve.
Add 0.5 g
K2HPO4 , 0.2g
MgSO4.7H2O, 0.2
g NaCl, 0.2 g
CaCl2.6H2O, 10 g
mannitol and 0.4
g yeast extract.
Dispense as
required and
sterilize.

 
     e. Glucose  Make up nutrient
Nutrient Broth broth as already
directed and add
10 g per litre of
glucose.