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Exercise 4: Endocrine System Physiology: Activity 1: Metabolism and Thyroid Hormone Lab Report
2. Thyroxine is
You correctly answered: c. the most important hormone for maintaining the metabolic rate and body temperature.
4. An injection of TSH to an otherwise normal animal will cause which of the following?
You correctly answered: d. goiter development
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Experiment Results
Predict Question:
Predict Question 1: Make a prediction about the basal metabolic rate (BMR) of the remaining rats compared with the BMR
of the normal rat you just measured.
Your answer : c. The BMR of both remaining rats will be lower than the normal rat's BMR.
Predict Question 2: What do you think will happen after you inject thyroxine into the three rats?
Your answer : d. The normal rat will become hyperthyroidic but will not develop a goiter.
Predict Question 3: What do you think will happen after you inject TSH into the three rats?
Your answer : a. The normal rat will become hyperthyroidic and develop a goiter.
Predict Question 4: Propylthiouracil (PTU) is a drug that inhibits the production of thyroxine by blocking the attachment of
iodine to tyrosine residues in the follicle cells of the thyroid gland (iodinated tyrosines are linked together to form thyroxine).
What do you think will happen after you inject PTU into the three rats?
Your answer : c. The hypophysectomized rat will become hypothyroidic and develop a goiter.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 420 ml O2/hr
1i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.68 ml O2/kg/hr
2h. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 372 ml O2/hr
2i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
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Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1,51 ml O2/kg/hr
3h. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
3i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.55 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
4c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 498 ml O2/hr
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4d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.9 ml O2/kg/hr
5c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 468 ml O2/hr
5d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.9 ml O2/kg/hr
6c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 462 ml O2/hr
6d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.89 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
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Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
Which of the rats developed a goiter after injection with thyroxine (select all that apply)?
You correctly answered: d. None of the rats developed a goiter with this injection.
7c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 480 ml O2/hr
7d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.92 ml O2/kg/hr
8c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
8d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
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You answered: 1.53 ml O2/kg/hr
9c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 468 ml O2/hr
9d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.91 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the TSH injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the TSH injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the TSH injection.
Which of the rats developed a goiter after injection with TSH (select all that apply)?
You correctly answered: a. the normal rat c. the hypophysectomized rat
10c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
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10d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.51 ml O2/kg/hr
11c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
11d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1.54 ml O2/kg/hr
12c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
12d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1,54 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
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Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
Which of the rats developed a goiter after injection with PTU (select all that apply)?
You correctly answered: a. the normal rat
Experiment Data:
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Post-lab Quiz Results
You scored 71% by answering 5 out of 7 questions correctly.
1. How would you treat a thyroidectomized animal so that it functions like a "normal" animal?
Your answer: b. Provide the animal TSH supplements.
Correct answer: c. Provide the animal T4 supplements.
2. As a result of the missing hormone(s) in the hypophysectimized rat, what would be some expected symptoms?
You correctly answered: d. decreased basal metabolic rate
3. An injection of thyroxine to an otherwise normal rat will cause which of the following?
You correctly answered: b. hyperthyroidism
4. Why didn't any of the rats develop a goiter after thyroxine injection?
You correctly answered: a. In all cases, TSH levels were not elevated by the thyroxine injection.
5. Why did the normal rat develop a palpable goiter with the TSH injection?
Your answer: b. The amount of T4 secretion by the thyroid gland became excessive.
Correct answer: c. The TSH receptors on the thyroid gland were excessively stimulated.
6. An injection of propylthiouracil to an otherwise normal animal will cause which of the following?
You correctly answered: b. goiter development
7. Why did the normal rat develop a palpable goiter with the propylthiouracil injection?
You correctly answered: d. The injection decreased the negative feedback mechanism on TSH.
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Review Sheet Results
You have not completed the Review Sheet.
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