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Exercise 4: Endocrine System Physiology: Activity 1: Metabolism and Thyroid Hormone Lab Report
2. Thyroxine is
You correctly answered: c. the most important hormone for maintaining the metabolic rate and body temperature.
4. An injection of TSH to an otherwise normal animal will cause which of the following?
You correctly answered: d. goiter development
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Experiment Results
Predict Question:
Predict Question 1: Make a prediction about the basal metabolic rate (BMR) of the remaining rats compared with the BMR
of the normal rat you just measured.
Your answer : c. The BMR of both remaining rats will be lower than the normal rat's BMR.
Predict Question 2: What do you think will happen after you inject thyroxine into the three rats?
Your answer : a. The normal rat will become hyperthyroidic and develop a goiter.
Predict Question 3: What do you think will happen after you inject TSH into the three rats?
Your answer : a. The normal rat will become hyperthyroidic and develop a goiter.
Predict Question 4: Propylthiouracil (PTU) is a drug that inhibits the production of thyroxine by blocking the attachment of
iodine to tyrosine residues in the follicle cells of the thyroid gland (iodinated tyrosines are linked together to form thyroxine).
What do you think will happen after you inject PTU into the three rats?
Your answer : b. The thyroidectomized rat will become hypothyroidic and develop a goiter.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 426 ml O2/hr
1i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 426 ml O2/kg/hr
2h. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 372 ml O2/hr
2i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
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Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1524 ml O2/kg/hr
3h. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
3i. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1524.85 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800).
4c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 510 ml O2/hr
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4d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 2031.87 ml O2/kg/hr
5c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 468 ml O2/hr
5d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1918.03 ml O2/kg/hr
6c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 474 ml O2/hr
6d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1934.69 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
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Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the thyroxine injection.
Which of the rats developed a goiter after injection with thyroxine (select all that apply)?
You correctly answered: d. None of the rats developed a goiter with this injection.
7c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 486 ml O2/hr
7d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1936.25 ml O2/kg/hr
8c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
8d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
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You answered: 1549.18 ml O2/kg/hr
9c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 468 ml O2/hr
9d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1910.20 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the TSH injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the TSH injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the TSH injection.
Which of the rats developed a goiter after injection with TSH (select all that apply)?
Your answer: a. the normal rat
Correct answer: a. the normal rat c. the hypophysectomized rat
10c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
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Report.
You answered: 378 ml O2/hr
10d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1505.98 ml O2/kg/hr
11c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 372 ml O2/hr
11d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1524.59 ml O2/kg/hr
12c. Calculate the oxygen consumption per hour for this rat using the following equation.
Enter the oxygen consumption per hour in the field below and then click Submit Data to display your results in the Lab
Report.
You answered: 378 ml O2/hr
12d. Now that you have calculated the oxygen consumption per hour for this rat, you can calculate the metabolic rate per
kilogram of body weight with the following equation (note that you need to convert the weight data from grams to kilograms
to use this equation).
Enter the metabolic rate in the field below and then click Submit Data to display your results in the Lab Report.
You answered: 1542.86 ml O2/kg/hr
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
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The normal rat is
You correctly answered: a. hypothyroid
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
Judging from their basal metabolic rates (an indicator of thyroid function), categorize the rats as hypothyroid (low thyroid
levels; BMR below 1600), euthyroid ("good," or normal, thyroid levels; BMR = 1650-1750), or hyperthryoid (high thyroid
levels; BMR above 1800) after the PTU injection.
Which of the rats developed a goiter after injection with PTU (select all that apply)?
Your answer : d. None of the rats developed a goiter with this injection.
Correct answer: a. the normal rat
Experiment Data:
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Post-lab Quiz Results
You scored 100% by answering 7 out of 7 questions correctly.
1. How would you treat a thyroidectomized animal so that it functions like a "normal" animal?
You correctly answered: c. Provide the animal T4 supplements.
2. As a result of the missing hormone(s) in the hypophysectimized rat, what would be some expected symptoms?
You correctly answered: d. decreased basal metabolic rate
3. An injection of thyroxine to an otherwise normal rat will cause which of the following?
You correctly answered: b. hyperthyroidism
4. Why didn't any of the rats develop a goiter after thyroxine injection?
You correctly answered: a. In all cases, TSH levels were not elevated by the thyroxine injection.
5. Why did the normal rat develop a palpable goiter with the TSH injection?
You correctly answered: c. The TSH receptors on the thyroid gland were excessively stimulated.
6. An injection of propylthiouracil to an otherwise normal animal will cause which of the following?
You correctly answered: b. goiter development
7. Why did the normal rat develop a palpable goiter with the propylthiouracil injection?
You correctly answered: d. The injection decreased the negative feedback mechanism on TSH.
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Review Sheet Results
1. Part 1: Determining the Basal Metabolic Rates
2. Why did the metabolic rates differ between the normal rat and the surgically altered rats? How well did the results
compare with your prediction?
Your answer:
Hal tersebut disebabkan karena organ yang berperan penting dalam menjaga kestabilan BMR ditiadakan (seperti tiroid atau
kelenjar pituitari)
3. If an animal has been thyroidectomized, what hormone(s) would be missing in its blood?
Your answer:
thyroxine
4. If an animal has been hypophysectomized, what effect would you expect to see in the hormone levels in its body?
Your answer:
Hewan tersebut akan kehilangan TSH sehingga tidak dapat menstimulasi thyroid untuk mensekresikan thyroxine
What was the effect of thyroxine injections on the normal rat's BMR?
Your answer:
Tikus tersebut mengalami hipertiroid
6. What was the effect of thyroxine injections on the thyroidectomized rat's BMR? How does the BMR in this case compare
with the normal rat's BMR? Was the dose of thyroxine in the syringe too large, too small, or just right?
Your answer:
Tx rat mengalami peningkatan BMR hingga sebesar 1918.03. Jika dibandingkan dengan tikus normal yang memiliki BMR
2031.87, tikus Tx memiliki BMR yang lebih rendah. Dosis tiroxine terlalu besar
7. What was the effect of thyroxine injections on the hypophysectomized rat's BMR? How does the BMR in this case
compare with the normal rat's BMR? Was the dose of thyroxine in the syringe too large, too small, or just right?
Your answer:
Hypophysectomized rat's BMR: 1934.69 yang lebih rendah jika dibandingkan dengan BMR tikus normal. Dosis tiroksin
terlalu besar
What was the effect of thyroid-stimulating hormone (TSH) injections on the normal rat's BMR?
Your answer:
Tikus normal menjadi hipertiroid
9. What was the effect of TSH injections on the thyroidectomized rat's BMR? How does the BMR in this case compare with
the normal rat's BMR? Why was this effect observed?
Your answer:
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Injeksi TSH tidak meningkatkan BMR secara signifikan. jika dibandingkan dengan BMR tikus normal, BMR Tx masih
tergolong hypotiroid
10. What was the effect of TSH injections on the hypophysectomized rat's BMR? How does the BMR in this case compare
with the normal rat's BMR? Was the dose of TSH in the syringe too large, too small, or just right?
Your answer:
Injeksi TSH meningkatkan BMR pada Hypox. Jika dibandingkan denngan BMR tikus normal, BMR tikus hypox masih
tergolong hipertiroid. Dosis TSH terlalu besar
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