OUR LADY OF FATIMA UNIVERSITY

COLLEGE OF PHARMACY
Valenzuela. Quezon City. Antipolo. Pampanga. Cabanatuan. Laguna

Pharmacology Laboratory
At the end of the unit, the
student should have
1.observed safe, humane
handling and restraint of
laboratory animals.
2.understood potential
hazards when handling
laboratory animals
3.identified
ways to prevent
hazards associated with
handling of laboratory
animals
In order to successfully complete Exercise 1, please do the following:
1.Attend: Online Orientation via Zoom
2.Read: Introduction to Exercise 1 and Introduction to Laboratory Mouse
3.Watch: Video links
4.Discuss: Join your classmates in a Group Discussion
5.Research: Work individually to answer worksheets in Exercise 1 and
answer Assignment no. 1
6.Quiz: Take the online Quiz No. 1
 Introduction

Animal Biosafety
Techniques Practices
Handling

Legal
Perspectives
The mouse is probably the most genetically and
biologically characterized mammal in the world.

Kingdom Animal
Phylum Chordata
Class Mammalia
Order Rodentia
Family Muridae
Subfamily Murinae
Genus Mus
Species musculus

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 Lifespan 1-3 years
Adult weight Males 20-30 g,
Females 18-35g
Birth weight 1-2 g
Heart rate 310-840 beats per minute
Body temperature 36.5-38°C
Respiratory rate 80-230 breaths per minute
Blood volume 7-8%, 1.5-2.5 ml
Urine volume 0.5-1 ml per day
Allergens Dander, urinary protein
• Laboratory Animals
• Animal Use
• Humane Care
• Physical Restraint

7
s

8
 Mus musculus
Rattus norvegicus

Oryctolagus cuniculus. Felis catus or Canis lupus familiaris

Felis silvestris catus
II. Biosafety practices in
animal handling
II. Biosafety Practices in Animal Handling
✓ Vaccination:
Tetanus toxoid

✓ Protective clothing and

equipment

✓ Animal handling and

restraint
 Biosafety Practices in Animal Handling
✓ Decontamination
• Microbial quality of all materials that will be
used should be considered at all times
- cage
- bedding
- rodent chow
- drinking water
- feeding bottle
 Biosafety Practices in Animal Handling
✓ Biohazardous Waste Disposal
❖ Sharps (e.g. glass implements, needles,
syringes, blades, etc. coming from facilities
using infectious materials )
❖ Blood, tissues

✓ Animal Waste Disposal

❖ carcasses and beddings:
✓ decontaminate in an autoclave and
throw in a yellow trash can
- UP National Institute of Health
- Research Institute of Tropical
Medicine (RITM)
- Department of Science and
Technology (DOST)
- Bureau of Food and Drug (BFAD)
- St. Luke’s Medical Center, Research
and Biotechnology Division (RBD)
III. LEGAL PERSPECTIVES IN ANIMAL
HANDLING
 REPUBLIC ACT NO. 8485:
AN ACT TO PROMOTE ANIMAL WELFARE IN THE
PHILIPPINES

SEC. 6. It shall be unlawful for any person to torture any

animal, to neglect to provide adequate care, sustenance or
shelter, or maltreat any animals or to subject any dog or
horse to dogfights or horsefights, kill or cause or procure to
be tortured or deprived of adequate care sustenance or
shelter, or maltreat or use the same in research or
experiments not expressly authorized by the Committee
on Animal Welfare.
IV. Techniques in Animal Handling
 Quarantine
 Health Monitoring
 Gender determination
 Animal identification methods
 Husbandry
 Handling and Restraint
 Anesthesia and Analgesia
 Quarantine
• hold the newly-arrived animals in a
separate area/cubicle
- to reduce the risk of introducing disease
- to observe the health condition of the
animals
- to acclimate the animals so they can
adopt to the new environment and food

• 3-7 days
 Health Monitoring
Normal: Signs of discomfort or
 active, have a disease:
smooth and shiny  Huddles in corner,
haircoat, bright hunched posture, scruffy
clear eyes, and haircoat, feels cool to the
good body mass. touch, sneezing
Compare the relative distance between anus and
urinary papilla.
Ear tags
logo or group Ear notching
number, animal punching the ear firmly
number and quickly

Fur dyes Implantable

Ear Tattoos microchips
Environmental conditions:
 Animals should be kept in rooms with the
temperature set at about 250C and humidity
at 50%.
 Lights should not be too bright since most
white rodents are albinos and too much light
hurts their eyes.
 They are diurnal which means they need
about 12 hours of light and 12 hours of
darkness each day.
Limit the number of animals per cage
BEDDINGS:
 Rice hulls
 Their bedding should be changed 2 or 3 times
a week to prevent the buildup of urea.
FOOD and WATER:
 They should have fresh rodent food and water
available at all times.
 https://www.youtube.com/watch?v=Gs-ebUnPQEc

Handling: Grasp the mouse by the base of the tail and lift it clear of its cage

Restraint: Grasp the scruff of the mouse between the thumb and the
forefinger while maintaining a firm grip on the tail
 Handling: Grasp the rat
around the shoulder and
then lift clear of its cage.

 Restraint: Thumb of the

handler should be
positioned under the
mandible of the rat while
the other fingers are
around the shoulder.
Hold the base of the tail
with the other hand.
 Handling: Grasp the
loose skin of the scruff with
one hand and placing
the other hand
underneath the belly

 Restraint: Grasp it firmly

by the skin overlying the
neck, not the ears.
Support the rest of the
body by placing the other
hand underneath the
bottom of the animal
• Anesthesia – loss of feeling or sensation, which is
often accompanied by loss of consciousness
• Analgesia – loss of sensitivity to painful
stimulation without loss of consciousness
• Necessary agents to be used and the manner of
administration should be known

• Free the animals from

pain and distress
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Thank you!
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