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MORES, MARY COLENE P.

BSMT 2G
MODULE 5 ENHANCEMENT QUESTIONS
CEREBROSPINAL FLUID (CSF)
1.Using a flowchart, provide the whole procedure of lumbar tap.

LUMBAR TAP PROCEDURE

Verify that no
contraindications exist.This Explain the procedure to the
Place the necessary orders
BEFORE THE may include doing a CT patient and answer all
so that the CSF tubes can be
head to rule out active questions. Obtain informed
PROCEDURE bleeding, midline shift, consent with appropriate
labeled after the procedure
is completed
space-occupying lesions or documentation.
signs of brain swelling.

Wash hands, open the


Position the patient either in Locate the L3/L4 space by
lumbar puncture tray
DURING THE lateral decubitus / fetal locating the superior iliac
without compromising
position, or sitting upright crests and placing your
sterility and consider any PROCEDURE leaning forward over a thumbs midline to the
extra supplies (i.e., spinal
small table spine.
needles or extra tubes)

Finish setting the LP tray


including opening the CSF Draw up and inject 10 mL of
Aseptically clean the skin Put on sterile gloves,
tubes in preparation to be 1% or 2% lidocaine
using chlorhexidine skin facemask, and protective
easily accessed, and apply (preservative free; without
prep gear per institutional policy
the sterile drapes to the epinephrine) to the area
patient

Insert the spinal needle The entry into the


directed at a slight cephalad subarachnoid space is
Attach the sterile
angle and with the bevel of commonly described as Have the patient slowly
manometer to the end of the
the needle oriented to the feeling a "pop" sensation, stretch out legs (if lying in
spinal needle to measure the
longitudinal fibers to the needle insert (obturator) lateral decubitus)
opening pressure
separate the fibers instead of is then removed and CSF
cutting them should begin to drip out

Reinsert the needle insert


Empty the manometer into
(obturator) and withdraw
CSF tube #1 and about 10 AFTER THE
Measure the closing the spinal needle and
drops of CSF into tubes #2 -
4 (note: some institutions
pressure (if indicated) immediately apply pressure PROCEDURE
and an adhesive bandage
use only 3 tubes)
over the insertion site

Document the procedure, Document the procedure,


Immediately label the CSF
Repeat neurologic number of attempts, number of attempts,
tubes have the tubes hand
assessment to evaluate for opening and closing opening and closing
carried/delivered to the lab
any changes post-LP pressure (if applicable), total pressure (if applicable), total
for analysis
amount of CSF drained amount of CSF drained
2.In a tabular form, give the cause and clinical significance of the different colors/appearance of CSF.

CSF COLOR CAUSE CLINICAL SIGNIFICANCE

Normal
CRYSTAL CLEAR

White Blood Cells (WBCs)(over Meningitis


HAZY, TURBID, MILKY,
200 cells/µl)
CLOUDY
Microorganisms Meningitis
(bacteria, fungi, amebas)
Proteins Disorders that affect blood-brain
barrier
Production of IgG within the CNS
Radiographic contrast media
OILY

Red Blood Cells (RBCs) Hemorrhage


BLOODY
Traumatic Tap
Hemoglobin Old hemorrhage
XANTHOCHROMIC
Lysed dells from traumatic tap
Bilirubin RBC degradation
Elevated serum bilirubin level
Carotene Increased serum levels
Protein Disorders affecting blood-brain
barrier
Melanin Meningeal melanosarcoma

REFERENCES

https://www.ebmconsult.com/articles/procedure-lumbar-puncture

https://slideplayer.com/slide/5846519/

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