ChE Department CIT University Why is microbial control necessary?
It is mainly to inhibit the growth of
pathogens. Sterilization • it refers to the removal or destruction of all microbes, including viruses and bacterial endospores, in or on an object
• is an absolute term that implies the
complete and total removal of all living things Aseptic • it describes an environment or procedure that is free of contamination by pathogens
• Ex. vegetables & fruit juices are available
in aseptic packaging; surgeons & lab technicians use aseptic techniques to avoid contamination while doing their job Disinfection • it refers to the use of physical or chemical agents known as disinfectants • this term is used only for treatment of inanimate objects Antisepsis • refers to a process of using chemical or antimicrobial agent on skin or other tissue • the chemical agent is called antiseptic Degerming • is the removal of microbes from a surface by scrubbing Sanitizing • is the process of disinfecting places and utensils used by the public to reduce the number of pathogenic microbes to meet accepted public health standards Pasteurization • is the use of heat to kill pathogens and reduce the number of spoilage microorganisms in food and beverages What is microbial death? Death – is a phenomenon that involves the permanent termination of an organism’s vital processes Factors that affect Death Rate 1) Number of microorganisms. 2) Nature of microorganisms. 3) Temperature & pH of the environment. 4) Concentration of the agent. 5) Mode of action of the agent. 6) Presence of solvents, interfering organic matter, and inhibitors. How Antimicrobial Agents Work? The cellular targets of physical & chemical agents fall into 4 general categories: 1. the cell wall 2. the cell membrane 3. cellular synthetic processes (DNA, RNA) 4. proteins Methods of Physical Control Heat Radiation Filtration Ultrasonic waves Cold HEAT Moist Heat: - hot water, boiling water, steam (vaporized water) - temperature ranges from 60-135 0C Dry Heat: - that has been heated by a flame or electric heating coil - temperature ranges from 1600C up What is the effect on microorganisms when moist heat is used as compared to dry heat? Moist heat generally coagulates and causes denaturation in microbes. In denaturation, proteins separate as an insoluble mass as they revert from their 3-dimensional structure to a 2-dimensional structure. In dry heat, the primary effect is due to oxidation of large molecules; is a less efficient process which requires a longer period of process time COLD “Cold merely retards the activities of most microbes.”
Lyophilization – a combination of freezing and
drying; a common method of preserving microorganisms and other cells Radiation Types of radiation that can be used as anti- microbial control agents: 1) Ionizing radiation – include x-rays and gamma rays, which form free radicals in cytoplasm and the free radicals destroy microbial proteins and DNA 2) Ultraviolet radiation – will effect nucleic acids by binding together adjacent thymine bases; microbes will die because DNA cannot function or replicate itself Ultrasonic waves These high-frequency sound waves causes vibrations that coagulate cellular proteins and disintegrate cellular components.
Ultrasonic vibrations are commonly used as
cleaning agent for lab materials and as a cell disrupters. Filtration Modern microbial filters: cellulose acetate; polycarbonate; plastic materials (teflon & nylon) where pores size can vary from coarse (8 microns) to ultrafine (0.02 micron) Applications of Filtration use to prepare liquids that cannot withstand heat, including serum & other blood products, vaccines, drugs, IV fluids, enzymes, and media use for decontaminating milk & beer without altering their flavor used in water purification used in removing airborne contaminants that are common source of infection & spoilage Chemical Agents Gases that can perform sterilization: • ethylene oxide (ETO) is used to sterile plastics (such as petri dishes) • beta propiolactone (BPL) is used to sterile liquids • formaldehyde can be used for various materials Qualities in choosing antimicrobial chemical agents: rapid action even in low concentration solubility in water or alcohol and long-term stability broad-spectrum microbicidal action without being toxic to human and animal tissues penetration of inanimate surfaces to sustain a cumulative or persistent action resistance to becoming inactivated by organic matter noncorrosive or nonstaining properties sanitizing and deodorizing properties affordability and ready availability Thank you !