Parameters Ascorbid acid sample Ascorbid Acid

Standard
structure

Chemical C6H6O6 C6H6O6

formula
Molecular 176,12 176,12
weight
Melting range 190-192 °C 190-194 °C
Storage Store in a refrigerator/protect from light, Temp 2-8°C
keep container tightly closed attachment of
a 20 mm alumunium crimp seal
recommended for used portions
Application Ascorbic Acid may be used
as a pharmaceutical reference
standard for the
quantification of the analyte
in pharmaceutical
formulations using
hydrophilic interaction
chromatography.
methode  HPLC  HPLC
Column: Tosoh Bioscience Super Column : Discovery C18, 15
AW2500, 6 x 150mm, 4µm cm x 4.6 mm I.D., 5 µm
Mobile Phase: 7.8g/L NaH2PO4 + 6.1g/L particles (504955)
Na2HPO4 in Water, column temp : ambient
pH = 2.5 mobile phase : [A] 50 mM
Flow Rate: 0.6mL/min potassium phosphate, dibasic,
Column Temperature: 30ºC pH 4.5 to methanol: [B]
Injection: 4µL 50mM potassium phosphate,
Detector: 245nm dibasic,
pH : 4.0 (30:70, A:B) in 8
 Infrared spectrophothometry min
flow rate : 1 mL/min
Injection : 1 µL
Detector : UV, 254 nm

 Gas Chromatography
(GC)

pharmacopeia Comparative assay traceable to BP 461

demonstrates direct traceable to PhEur A1300000
traceability
traceability to Pharmacopeial traceable to USP 1043003
Standards Specification:
99.0-100.5% (USP)