Experimental Cell Physiology

PSL1026 - Organization
• Zhong-Ping Feng
zp.feng@utoronto.ca (416) 946-0671

• Evelyn Lambe
evelyn.lambe@utoronto.ca (416) 946-0910

• Hong-Shuo Sun
hss sun@utoronto ca (416) 946
hss.sun@utoronto.ca 946-5506
5506 / 978
978-8848
8848

• Shuzo Sugita
ssugita@uhnres.utoronto.ca
it @ h t t /
/ssugita@uhnresearch.ca
it @ h h
(416) 603-5077
 Experimental Cell Physiology
PSL1026 - Organization

• Time & Place:

– 2:00 – 5:00, Tuesdays
– Room: MSB 3227 (with exceptions)

• Format:
– 3 Hours
H per week
k (Total:
(T t l 12 sessions)
i )
– Faculty lectures /class discussion (~50%)
– Demonstration/data analysis (~30%)
– Students
S d presentation
i ((~20%)
20%)

• Size:
– 16 students (4 students/group)
/
 Topics & Schedule
Week Topic Specifics Location
Sep. 20 Introductory meeting MSB 3227
(Feng, Lambe, Sugita & Sun)
Sep. 27 Electrophysiology Overview Microelectrode MSB 3227
(Feng) Recording techniques
Ion channels
Oct 4
Oct. Patch clamp recordings Whole-cell recording MSB 3227
(Feng) Single channel recording
(Data analysis)
Oct. 11 Patch clamp recordings Demo: Whole-cell recording MSB 3227
(Feng) & its applications
Oct. 18 Optophsiology - 1 Demo: Confocal microscopy MSB 3227
(Sun) Electron microscopy
Stereotaxic coordinates

Oct. 25 CNS and PNS: injury models Stroke & nerve injury MSB 3227
(Sun) Nerve conductance
 Topics & Schedule
Week Topic Specifics Location

Nov. 1 Optophysiology - 2 Multiphoton microscopy MSB 3227

(Lambe) Calcium imaging
Optogenetics
Nov. 8 Optophysiology - 3 Lab demos: Calcium MSB 3358
(Lambe) Imaging & Optogenetics

Nov. 15 Methods in Molecular Biology & Recombinant DNA MSB 3227

Protein chemistry (I) Gene expression
(Sugita) genomic manipulations
Protein assays
y
Nov. 22 Methods in Molecular Biology & Lab Demo 7KD-425
Protein chemistry (II) Krembil Res Ins
(Sugita)

Nov. 29 Student presentations MSB 3227

(Feng, Lambe, Sugita, Sun)
Dec. 6 Student presentations MSB 3227
(Feng Lambe
(Feng, Lambe, Sugita
Sugita, Sun)
 Course Objectives
(G l and
(Goals d Expectations)
E i )
• to understand techniques commonly used in cell
physiology
– related to multiple fields: neurobiology,
endocrinology cardiovascular
endocrinology, cardiovascular, respiratory
respiratory,
developmental, and reproductive physiology

• the principles, applications, and limitations of the

selected techniques

• to observe hands-on experiments with selected

techniques and related data analysis
 Marking Scheme
• Four research ppaper
p critiques
q
- 40% (10% each instructor)
• One oral presentation and Q & A
- 40%
• Class participation/discussion/attendance
- 20% (5% each instructor)

• Meet the deadlines

- Report submission
- Presentation on schedule
(2% deduction for each day of lateness)
 Research paper critiques
(40%)

• One paper critique from each session – the

paper will be given by individual instructors
• T t l 4 paper critiques,
Total iti 10% each
h iinstructor
t t
• Format: 1 page (single space)
• Deadline of submission: 2 weeks following topic
is given
• Detailed guidelines and evaluation criteria to
follow by
y individual instructors
 Student Academic Integrity
• http://www.artsci.utoronto.ca/osai
p
• Academic Integrity Checklist

• http://www.artsci.utoronto.ca/osai/The-rules
• What is Academic Misconduct:
• Plagiarism (Presenting the work, ideas, or words of
another as your own,
own even if by accident
accident.))
• Unauthorized resubmission (Submitting work you
have submitted before without obtaining permission
from your instructor.)
 Turnitin Assignment
(Portal)
• Students can create account with valid UoT email
address; ROSI

• Turnitn Assignments allow students to submit their

assignments as a single file or through a Cut & Paste
dialog.
 Statement of Using Turnitin
Normally,y, students will be required
q to submit their
course essays to Turnitin.com for a review of textual
similarity and detection of possible plagiarism. In
doing so, students will allow their essays to be
included as source documents in the Turnitin.com
reference database
database, where they will be used solely
for the purpose of detecting plagiarism. The terms
that apply to the University's
University s use of the Turnitin
Turnitin.com
com
service are described on the Turnitin.com web site.
Submission format of paper critiques

• PDF or Word file

• Turnitin
Turnitin.com
com should be used to before
submission

http://www.turnitin.com
 Oral presentations and Q & A
(40%)
• Topics given by 4 instructors, to address practical
questions related to (but not limited to) the
techniques discussed in the classes, such as
– Electrophysiology
– Optogenetics
– Optophysiology
– Molecular biology & protein chemistry
– Animal models of diseases

• Each group presents one topic, and asks questions to

one different topic
• Presentations will be evaluated by the instructors
and
d students
t d t
• Detailed guidelines and evaluation criteria to follow
 Class participation
(20%)

• 5% by each instructor
• Involvement in the class
• Class discussion/questions
• Specifics from each instructor
• Class attendance
 Background readings for
Electrophysiolgy
• Hille B.
B (2001) ‘Ion
Ion Channels of Excitable Membranes
Membranes’, 3rd
ed. Sinauer Associates, Inc. ISBN 0-87893-321-2

• Walz W
W. (2007) “Patch
Patch-Clamp
Clamp Analysis – Advanced
Techniques” Neuromethods Vol 38. 2nd Ed. Humana Press.
ISBN 978-1-58829-705-1

• Ogden, D. (1994) “Microelectrode Techniques”.

http://www.utdallas.edu/~tres/microelectrode/me.html

• The Axon Guide

http://www.moleculardevices.com/pages/instruments/axon_
guide.html