FOOD SYSTEM

Direct Identification of the main pathogenic

microorganisms from Foodstuffs

A B ✹ Microbial identification
1 2 3 4 1 2 3 4 Salmonella spp.
LDC H2S UR PRO LDC H2S UR PRO

Citrobacter spp.
* *
5
PSE
6
STA
7
ESC
8
IND
5
PSE
6
STA
7
ESC
8
IND Proteus spp./Providencia spp.
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* *
Pseudomonas spp.
9 10 11 12 9 10 11 12
BCE LIS CAT Y/M BCE LIS CAT Y/M
Staphylococcus aureus
Escherichia coli, E. coli 0157
FOOD SYSTEM
Sample A ...................................
Sample B ...................................
Bacillus cereus
Listeria spp.
A B Yeasts and moulds
1 2 3 4 1 2 3 4
LDC H2S UR PRO LDC H2S UR PRO

5
PSE
6
STA
7
ESC
8
IND
*
5
PSE
6
STA
7
ESC
8
IND
*
✹ Validated to ISO 16140
Microbiology of food and animal feeding stuffs:
* * protocol for the validation of alternative methods
9 10 11 12 9 10 11 12
BCE LIS CAT Y/M BCE LIS CAT Y/M
Listeria spp.
Salmonella spp.
FOOD SYSTEM
Sample A ...................................
Sample B ...................................

A B ✹ Benefits
1
LDC
2
H2S
3
UR
4
PRO
1
LDC
2
H2S
3
UR
4
PRO
✓ Inoculation from foodstuffs suspension
✓ Conveniently designed for two tests
* *
5
PSE
6
STA
7
ESC
8
IND
5
PSE
6
STA
7
ESC
8
IND ✓ Results ready in 18-24 hours
** * ✓ Clear and well defined color changes
9 10 11 12 9 10 11 12

✓ Easy interpretation
BCE LIS CAT Y/M BCE LIS CAT Y/M

FOOD SYSTEM
Sample A ...................................
Sample B ...................................

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 FOOD SYSTEM
Results Test Procedure
Identification

Salmonella spp.
1 2 3 4 1 2 3 4 • positive
LDC H2S UR PRO LDC H2S UR PRO agglutination test

Citrobacter spp.
• negative
1 2
1 2 3 4 1 2 3 4
LDC H2S UR PRO LDC H2S UR PRO agglutination test
•Homogenize a quantity of foodstuff •Transfer 10 mL of homogenized
(1, 10 or 25 g) in an appropriate sample into a suitable tube.
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Proteus spp. volume (9, 90 or 225 mL) (dil. 1:10)

1 2 3 4 1 2 3 4 Providencia spp. of BUFFERED PEPTONE WATER
LDC H2S UR PRO LDC H2S UR PRO
or RINGER’S Solution. •Incubate at 36±1°C for 4-6 hours.
The final dilution of the sample
should be 1:10.

Pseudomonas spp.
5 5 • positive oxidase test
PSE PSE

Staphylococcus aureus
6 6
• positive agglutination test
STA STA

* *
Escherichia coli
* positive indole test
3 4
7 8 7 8
ESC IND ESC IND • positive agglutination test •Transfer 0.5 mL of sample •Distribute 0.2 mL of suspension
for E. coli 0157 suspension into a vial of into each well of the system.
Physiological Solution
contained in the kit. •Cover the wells 1-LDC, 2-H2S
and 3-UR with one drop of
Bacillus cereus vaseline oil and incubate at 36±1°C
9 9
• culture medium confirmation for 18-24 hours.
BCE BCE

*
Listeria spp.
*
10 11 10 11 * positive catalase test
LIS CAT LIS CAT • culture medium confirmation

Yeasts and Moulds

12 12
- confirm at the microscope
Y/M Y/M

DESCRIPTION PACKAGING REF. 5 6

•Add 2 drops of Kovac’s Reagent •Confirm Yeasts and Moulds by
FOOD SYSTEM 40 tests 71680 to the well 8-IND for confirming taking a drop from the well
H2O2 REAGENT 10 mL 80057 the relevant ID. 12-Y/M and watch for spores
at the microscope (400x).
KOVAC’S - REAGENT 4X25 mL 80271 •Add 2 drops of Reagent H2O2 •Note down the test results on
to the well 11-CAT and watch for the form provided in the kit,
OXIDASE TEST STICK 50 sticks 88029 the formation of bubbles. or read and interpret with
automatic readers.
VASELINE OIL 4X50 mL 80279

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Fax : +39 0858930330 ref. 6551966 - Rev. 2/ 29.10.2012
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