91. To cut the DNA with restriction endonuclease en- 91.
91. Mh-,u-,- dks izfrca/ku ,aMksU;qfDy,t ,atkbe ls dkVus esa
zyme it would be need that :
(1) Palindromic sequences are present in DNA
(2) It would be free from other macromolecules
(3) It would have selectable marker
(4) More than one options are correct
92. Agarose gel used in gel electrophoresis is :
(1) Artificial polymer extracted from sea weeds
(2) Natural polymer extracted from sea weeds
(3) Natural polymer extracted from sea fungi
(4) Polymer extracted from bacteria
93. Which of the following is not a tool of genetic engi-
neering?
(1) Cloning vector (2) Restriction enzyme
(3) Foreign DNA (4) GMO
94. Which of the following is wrong match :
(1) Non-recombinants ® Produce blue coloured
colonies in presence of chromogenic substance
containing medium.
(2) Agrobacterium fumifaciens ® Transform nor-
mal plant cells into tumor and used in genetic en-
gineering as vector
(3) Bacteriophage DNA ® Not have ability to repli-
cate into bacterial cells independent to bacterial
chromosome used as vector in genetic engineer-
ing
(4) Transformation ® Transfer of DNA into bacte-
rium through which phenotype of bacterium is
changed
95. Instructure of PBR322 plasmid vector :
(1) ori-genes are responsible for ligation of alien DNA
(2) rop-genes encode for proteins that activates the
selectable markers
(3) Transformants are identified by tetr or ampr genes
by any one
(4) Pvu I and Pvu II are present in ampr gene seg-
ment
96. For which of the following enzyme recognition sites
are not avilable on PBR322 plasmid :
(1) Cla I and Eco RI (2) Hind III and Sal I
(3) Hind II and Eco R II (4) Pvu I and Pst I
, Statue Circle JNV Colony, Bikaner Mob. : 9358000151, 9358000152
fuEu dh vko';drk gksrh gS -
(1) Mh-,u-,- esa iSyhUMksfed vuqØe mifLFkr gks
(2) ;g vU; nh?kZ v.kqvksa ls eqDr gksuk pkfg,A
(3) ml ds ikl oj.k ;ksX; fpUgd gksus pkfg,A
(4) ,d ls vf/kd fodYi lgh gSA
92. tsy bysDVªkQs ksjfs ll esa mi;ksx gksus okys ,sxkjkst tsy %
(1) leqnzh ?kkl ls fudkyk x;k Ñf=e cgqyd gSA
(2) leqnzh ?kkl ls fudkyk x;k çkÑfrd cgqyd gSA
(3) leqnzh dodksa ls fudkyk x;k çkÑfrd cgqyd gSA
(4) thok.kqvksa ls fudkyk x;k cgqyd gSA
93. fuEu esa ls dkSu vuqokaf'kd vfHk;kaf=dh dk lk/ku ugha gS%
(1) Dyksfuax okgd (2) jsfLVªD'ku ,Utkbe
(3) ckg~; DNA (4) GMO
94. fuEu esa ls dkSulk ;qXe xyr lqesfyr gS &
(1) viuqZ;ksxtksa ® ek/;e esa o.kksZadksRikndh inkFkZ dh
mifLFkrh esa uhys jax dh dkWyksuh dk fuekZ.k gksrk gSA
(2) ,xzkcs SDVhfj;e V~;ew hQsf'k,al ® lkekU; ikni dksf'kdk
dks vcqZn esa :ikUrfjr djrk gS rFkk vkuqokaf'kd
rdfudh esa okgd dk dk;Z djrk gSA
(3) thok.kqHkksth Mh-,u-,-® thok.kq dksf'kdkvksa esa thok.kq
xq.klw= ls Lora= izfrÑfr djus esa l{ke ugha gksrk
rFkk vkuqokaf'kd rduhdh esa okgd dk dk;Z djrk
gSA
(4) :ikUrj.k ® Mh-,u-,- thok.kq dksf'kdk esa LFkkukUrj.k
ftlls thok.kqvksa dk fQuksVkbZi cnyrk gSA
95. PBR322 okgd IyktfeM lajpuk esa -
(1) ckgjh Mh-,u-,u- dk ori-genes ds lkFk ligation
çØe ds fy, mÙkjnk;h gSA
(2) rop-genes çksVhUk dks dksM djrk gS] tks oj.k ;ksX;
fpUgdksa dks lfØ; djrk gSA
(3) :ikUrjtksa dks fuEUk ,d tetr ;k ampr thu }kjk
igpkuk tkrk gSA
(4) Pvu I rFkk Pvu II ampr thu esa mifLFkr gksrs gSA
96. fuEu esa ls fdl ,Utkbe ds fy, PBR322 IykfTeM okgd
ij igpku LFky vuqifLFkr gksrs gS &
(1) Cla I rFkk Eco RI (2) Hind III rFkk Sal I
(3) Hind II rFkk Eco R II (4) Pvu I rFkk Pst I
[17]
97. During gel electrophoresis sieving effect is take place:
(1) Through the anode and cathode electrodes
(2) Through the pores of agarose gel
(3) Through the filaments of agarose gel
(4) All of these
98. Consider the given statements :
(A) Type II restriction exonuclease enzymes are used
in r-DNA technology for fragmentation of DNA
(B) C-DNA is obtained by the activity of RNA de-
pendent DNA polymerase enzyme activity
(C) Polymerisation of nucleotides in PCR by taq-
DNA polymerase take place at 72ºC tempera-
ture
(D) The process of gene cloning during r-DNA tech-
nology is take place in-vivo and in vitro type
(E) In bioreactors large scale production of culture
medium is take place during r-DNA technology.
How many statements are incorrect :
(1) 2 (2) 3 (3) 1 (4) 4
99. Which is one is not an advantage of bioreactor :
(1) Oxygen delivery system
(2) Foam control system
(3) Expensive to run it
(4) Avaliability of nutrients
100. Identify and match the labelled items A, B, C, D, E, F
and G in the diagram below from the list I-VII given
with components.
97. tsy bysDVªkQ
s kWjfs ll esa Nyuh izHkko gksrk gS &
(1) ,uksM rFkk dSFkksM bysDVªkWM ds ek/;e ls
(2) ,xkjkst tsy ds fNnz ds ek/;e ls
(3) ,xkjkst tsy ds rarqvksa ds ek/;e ls
(4) mijksDr lHkh
98. fn;s x;s dFkuksa ij fopkj djsa &
(A) Type II restriction exonuclease enzymes dk
mi;ksx r-DNA rduhdh esa DNA dks [kf.Mr djus
esa gksrk gS
(B) C-DNA dks RNA dependent DNA polymerase
,Utkbe vfHkfØ;k }kjk izkIr fd;k tkrk gSA
(C) PCR esa U;wfDy;ksVkbMl dk cgqydhdj.k taq-DNA
polymerase ,Utkbe }kjk 72ºC rki ij gksrk gSSA
(D) thu Dyksfuax izØe r- DNA rduhdh }kjk bu&fooks
rFkk bu&foVªks izdkj ls gksrk gSA
(E) ck;ksfj,DVj }kjk lao/kZu ek/;e cM+s iSekus ij r-
DNA rduhdh }kjk mRikfnr gksrk gSA
fuEu esa ls vlR; dFku gS &
(1) 2 (2) 3 (3) 1 (4) 4
99. fuEu esa ls dkSulk ,d ck;ksfj,DVj dk ykHk ugha gS
(1) vkWDlhtu forj.k iz.kkyh
(2) >kx fu;a=.k iz.kkyh
(3) mi;ksx esa ysuk eagxk gS
(4) [kfutksa dh miyC/krk
100. fn;s x;s fp= esa A, B, C, D, E, F rFkk G dks fpUghr dj
lwph I-VII ds lkFk lqesfyr dhft,-

I. DNA polymerase II. Plasmid I. DNA ikWyhejst II. IykfTeM

III. Plasmid with 'sticky ends' III. fpifpis Nksj ;qDr IykfTeM
IV. DNA ligase IV. DNA ykbxst
V. Restriction endonuclease V. izfrca/ku ,.MksU;wfDy,t
VI. Recombinant DNA VI. iquZ;ksxt DNA
VII.Reverse transcriptase VII. fjolZ VªkalfØiVsl
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 The correct components are : lfg fodYi gS &
A B C D E F G A B C D E F G
(1) VII I II V III IV VI (1) VII I II V III IV VI
(2) VII VI V IV III II I (2) VII VI V IV III II I
(3) VII V III I II IV VI (3) VII V III I II IV VI
(4) I II IV VI III V VII (4) I II IV VI III V VII
101. Primers are : 101. miØked gS -
(1) Small chemically synthesised oligonucleotides of (1) NksVs jklk;fud la'ys"kh vYiU;wfDy;ksVkbM yxHkx
about 10-18 nucleotides that are complementary 10&18 U;wfDy;ksVkbM ls fufeZr tks VsIysV Mh-,u-,-
to the region of template DNA ds iwjd gksrs gSA
(2) Chemically synthesised oligonucleotides of about (2) NksVs jklk;fud la'ys"kh vYiU;wfDy;ksVkbM yxHkx
10-18 nucleotides that are not complementary to 10&18 U;wfDy;ksVkbM ls fufeZr tks VsIysV Mh-,u-,-
the region of template DNA ds iwjd ugha gksrs gSA
(3) The double-stranded DNA that need to be am- (3) f}lw=h DNA ftldks izof/kZr djokuk vko';d gksrk
plified gS
(4) Specific sequences present on recombinant DNA (4) fof'k"V vuqØe tks iqu; Z ksxt DNA ij mifLFkr gksrs
102. Stirred-tank bioreactors have been designed for : gS
(1) Purification of the product 102. foyksfMr gkSt ck;ksfj,DVj fuEu ds fy, :ikafdr gksrs gS
(2) Addition of preservatives to the product (1) mRikn ds 'kqf)dj.k ds fy,
(3) Availability of oxygen throughout the process (2) mRikn esa ifjj{kdksa ds feJ.k ds fy,
(4) Ensuring anaerobic conditions in the culture ves- (3) laiw.kZ izØe esa vkWDlhtu dh miYc/krk ds fy,
sel (4) ço/kZu iksr esa vok;qoh; ifjfLFkfr lajf{kr djus ds fy,
103. Which of the following statements are correct for 103. fuEu esa ls dkSulk dFku ,Utkbetaq ikWfyejst ds lanHkZ
the enzyme taq polymerase ? esa lR; gS -
I. Taq polymerase is thermally unstable. I. Taq ikWfyejst rki vLFkk;h gS
II. It requires primers for carrying out the process II. bldks cgqydh izØe ds fy, mikØedksa dh vko';drk
of polymerisation. gksrh gSA
III. Taq polymerase is isolated from thermophilic bac- III. Taq ikWfyejst dk i`FkDdj.k rki LFkk;h thok.kq
terium, Thermus aquaticus. FkeZl ,DosfVdl ls gksrk gSA
Choose the correct option : lgh dFku pqfu;s &
(1) I and II (2) I and III (1) I rFkk II (2) I rFkk III
(3) II and III (4) I, II and III (3) II rFkk III (4) I, II rFkk III
104. In case of polymerase chain reaction, temperature 104. ikWfyejst Ja`[kyk vfHkfØ;k ds pj.kksa esa fuEUk rki dh
required for the steps : vko';drk gS -
A. Dnaturation A. foÑrhdj.k
B. Annealing B. rkikuq'khyu
C. Extension C. izlkj
(1) A-94ºC, B-40ºC, C-72ºC (1) A-94ºC, B-40ºC, C-72ºC
(2) A-40ºC, B-72ºC, C-94ºC (2) A-40ºC, B-72ºC, C-94ºC
(3) A-72ºC, B-94ºC, C-40ºC (3) A-72ºC, B-94ºC, C-40ºC
(4) A-94ºC, B-72ºC, C-40ºC (4) A-94ºC, B-72ºC, C-40ºC

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105. The components of a biorector are :
I. An agitator system.
II. An oxygen delivery system.
III. Foam control system.
IV. Temperature control system.
V. pH control system.
VI. Sampling ports to with draw cultures periodically.
Choose the correct option :
(1) I, II, III, IV and V (2) II, IV, V and VI
(3) I, II, III, IV and VI (4) All of these
106. Plasmid are suitable vectors for gene cloning because:
(1) These are small circular DNA molecules, which
can integrate with host chromosomal DNA
(2) These are small circular DNA molecules with
their own replication origin site
(3) These can shuttle between prokaryotic and eu-
karyotic cells
(4) These often carry antibiotic resistance genes
107. 5'-GGATCC-3' is the recognition sequence and cleav-
age site for which of the following restriction endo-
nuclease ?
(1) Alu I (2) BamH I
(3) EcoR I (4) Hind III
108. In recombinant DNA technology, a plasmid vector is
cleaved by
(1) modified DNA ligase
(2) a heated alkaline solution
(3) the same enzyme that cleaves the donor DNA
(4) the different enzyme than that cleaves the donor
DNA.
109. Find out the incorrect statement :
(1) In host cell desired gene replication depends on
the host DNA polymerase
(2) Paul Berg is the father of genetic engineering
(3) Stanley Cohen and Herbert Boyer constructed
the first recombinant DNA
(4) Plasmid are autonomously replicating circular
single stranded DNA
105. ck;ksfj,DVj ds ?kVd gksrs gS -
I. iz{kksHkd ra=
II. vkWDlhtu forj.k iz.kkyh
III. >kx fu;a=.k iz.kkyh
IV. rki fu;a=.k iz.kkyh
V. pH fu;a=.k iz.kkyh
VI. izfrp;u iz}kj ftlls lao/kZu le;≤ ij fudkyk
tkrk gSA
lgh dFku pqfu;s &
(1) I, II, III, IV rFkk V (2) II, IV, V rFkk VI
(3) I, II, III, IV rFkk VI (4) mijksDr lHkh
106. thu Dyksfuax ds fy, IykfTeM mi;qZDr okgd gS] D;ksafd
(1) ;g NksVs xksykdkj v.kq gksrs gSA tks ijiks"kh xq.klw=h;
DNA esa lfEefyr gks tkrs gSA
(2) ;g NksVs xksykdkj DNA v.kq gksrs gSaA ftud viuk
izfrÑfr;u mRifr LFky gksrk gS
(3) ;g izkd s sfj;ksV rFkk ;qdfs j;ksV ds e/; okgd dk dk;Z
djrs gS
(4) buesa vDlj izfrtSfod izfrjks/kd thu mifLFkr gksrh gS
107. fuEu esa ls dkSuls izfrcaf/kr ,.MksU;wfDy;st ds fy,
5'-GGATCC-3' igpku Øe o fonyu LFky gS \
(1) Alu I (2) BamH I
(3) EcoR I (4) Hind III
108. fjdkEchusUV DNA VsDukykWth esa IykfTeM okgd dks dkVk tkrk
gS%
(1) :ikUrfjr DNA ykbxst }kjk
(2) ,d xeZ {kkjh; foy;u }kjk
(3) mlh ,Utkbe }kjk ftlls nkrk DNA dks dkVk tkrk gS
(4) nkrk DNA dks dkVus okys ,Utkbe ls fHkUu ,Utkbe }kjk
109. xyr dFku dk irk yxk;sa&
(1) ijiks"kh dksf'kdk esa okafNr thu dk izfrÑfrdj.k ijiks"kh
dksf'kdk ds DNA ikWyhesjt ij fuHkZj djrk gS
(2) vkuqofa 'kd vfHk;kaf=dh ds tud iksy cxZ Fks
(3) LVsuys dksgu s o gcZV cks;j us izFke iqu;ksZxt DNA
dks cuk;k Fkk
(4) IykfTeM Lor% izfrÑfr o`Ùkkdkj ,dy jTtqd DNA
gS

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110. How restriction endonucleases differ from exonu- 110. izfrcaf/kr ,.MksU;wfDy;st fdl izdkj ,DlksU;wfDy;st ls
cleases? fHkUu gS \
(1) They act on single strand of dsDNA (1) ;s dsDNA ds ,dy jTtqd ij dk;Z djrs gSa
(2) They cleave DNA at any point except the termi- (2) ;s vafre Nksj ds vfrfjDr fdlh Hkh fcUnq ij DNA
nal ends dk fonyu djrs gSa
(3) They remove the nucleotides from the ends of (3) ;s DNA ds Nksj ls U;wfDy;ksVkbM dks foyksfir djrs
DNA gSa
(4) Both (1) and (3) (4) (1) o (3) nksuksa
111. Read the given statements : 111. fn, x, dFkuksa dks if<+;s&
(A) Agrobacterium tumerfaciens is commonly called (A) ,xzkcs SDVhfj;e V~;ew hQslh;Ul dks izkÑfrd vkuqofa 'kd
as natural genetic engineer vfHk;Urk dgk tkrk gSA
(B) Chloramphenicol and Kanamycin are not usefull (B) E.coli es a Chloramphenicol rFkk Kanamycin
selectable markers in E.coli mi;ksxh oj.k ;ksX; fpUgd ugha gSA
(C) Ethidium bromide provides yellow colour to (C) ,sfFkfM;e czksekbM U;wfDyd vEy dks ihyk jax iznku
nucleic acids djrk gSA
(D) Stickiness of the DNA ends facilitates the activ- (D) DNA ds fpifpis Nksj DNA ikWyhesjt ,Utkbe dh
ity of enzyme DNA polymerase vfHkfØ;k esa lgk;d gksrs gSaA
Incorrect statements are : vlR; dFku gS&
(1) A and B (2) A and C (1) A rFkk B (2) A rFkk C
(3) B, C and D (4) A, C and D (3) B, C rFkk D (4) A, C rFkk D
112. Read the given statements regarding to endonuclease 112. izfrca/ku ,atkbe ds lanHkZ esa fuEu fn, x, dFkuksa dks
enzyme : if<+;s&
(A) Hind II has recongnition sequence of 8 base pairs (A) Hind II ,Utkbe ds fy, fpUgd LFky 8 {kkj ;qXeksa
dk gksrk gSA
(B) Now more than 230 restriction enzymes are pro- (B) vkt 230 ls vf/kd izfrca/ku ,Utkbe 900 LVªsu ls
duced from 900 strains fufeZr gSA
(C) Methylation enzyme protects DNA from restric- (C) Methylation ,Utkbe DNA dks izfrca/ku ,Utkbe ls
tion enzymes j{kk djrk gSA
(D) Palindromic nucleotide sequences are not impor- (D) Palindromic U;wfDy;ksVkbM vuqØe izfrca/ku ,Utkbe
tant for the activity of restriction enzymes dh fØ;kvksa ds fy, vko';d ugha gSA
Incorrect statements are : vlR; dFku gS&
(1) A and B (2) A and C (1) A rFkk B (2) A rFkk C
(3) B, C and D (4) A, B and D (3) B, C rFkk D (4) A, B rFkk D
113. Bioreactors are useful in : 113. ck;ksfj;sDVj fuEu izØeksa ds fy, mi;ksxh gksrs gSa&
(1) Separation and purification of a product (1) mRikn dk i`FkDdj.k o 'kks/ku esa
(2) Processing of large volumes of culture (2) vf/kd ek=k esa lao/kZu izfØ;k esa
(3) Micro-injection (3) lw{e var%{ksi.k
(4) Isolation of genetic material (4) vkuqokaf'kd inkFkZ dk i`FkDdj.k

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114. Some foreign DNA fragment is attached to sal I site 114. dqN okafNr DNA [k.Mksa dks pBR322 okgd dh sal I
of pBR322. This recombinant vector is used to tran- LFky ij tksM+k x;k rFkk bl iquZ;ksxt okgd dks ijiks"kh
sition Escherichia coli host cells. The cells subjected Escherichia coli dksf'kdk esa LFkkukUrfjr fd;k x;kA
to transformation are plated on two different media- :ikUrfjr dksf'kdkvksa dks nks vyx&vyx lao/kZu ek/;e
one containing ampicillin and the other containing esa o`f) djokbZ x;h tksfd ,d ek/;e esa ampicillin ;qDr
tetracyline. The transformed cells containing the re- rFkk nwljk ek/;e tetracyline ;qDr FkkA :ikUrfjr dksf'kdk
combinant vector : tks iquZ;ksxt okgd ;qDr gS&
(1) Will grow on both tetracycline containing and (1) nksuksa tetracycline rFkk ampicillin ;qDr ek/;e esa
ampicillin containing media o`f) djsx a s
(2) Will not grow on either tetracycline containing or (2) nksuksa tetracycline rFkk ampicillin ;qDr ek/;e esa
ampicillin containing media o`f) ugha djsaxs
(3) Will grow on tetracycline, but not on ampicillin (3) tetracycline ;qDr ek/;e esa o`f) djsx a s ij ampicillin
containing medium ;qDr ek/;e esa o`f) ugha djsx
a s
(4) Will grow on ampicillin, but not on tetracycline (4) ampicillin ;qDr ek/;e esa o`f) djsxa s ij tetracycline
containing medium ;qDr ek/;e esa o`f) ugha djsx
a s
115. pBR322, which is frequently used as a vector for clon- 115. pBR322 ftldks yxkrkj okgd ds rkSj ij thu Dyksfuax
ing gene is E.coli is a/an : esa E.coli dksf'kdk ds fy, mi;ksx esa fy;k tkrk gS&
(1) Original bacteria plasmid (1) ewy:i thok.kq IykfTeM gS
(2) Modified bacterial plasmid (2) :ikUrfjr thok.kq IykfTeM gS
(3) Viral genome (3) fo"kk.kq thukse gS
(4) Transposon (4) Vªkl a ikstksUl gS
116. Cohen and Boyer isolated an antibiotic resistance 116. Cohen rFkk Boyer us ,d izfrtSfod izfrjks/kd thu dks
gene, by cutting out a piece of DNA from a plasmid ,d IykfTeM esa ls i`FkDdj.k fd;k tksfd izfrtSfod
which was responsible for conferring antitiotic resis- izfrjks/kd {kerk iznku djus esa mÙkjnk;h Fkk] fuEu o"kZ esa
tance, in the year : i`FkDdj.k fd;k x;k&
(1) 1962 (2) 1965 (3) 1972 (4) 1982 (1) 1962 (2) 1965 (3) 1972 (4) 1982
117. Amplification of gene of interest by using DNA poly- 117. okafNr tho dk izof/kZr DNA polymerase ds }kjk fuEu
merase may go up to : la[;k rd laHko gS&
(1) 0.1 million times (2) 1.0 million times (1) 0.1 million times (2) 1.0 million times
(3) 1.0 billion times (4) 1.0 trillion times (3) 1.0 billion times (4) 1.0 trillion times
118. The restriction enzyme(s) used in recombinant DNA 118. iquZ;kstd DNA rduhdh esa DNA dks izfrcaf/kr ,atkbe
technology making staggered cuts in DNA leaving }kjk dkVdj fpifpis Nksj fufeZr fd;s tkrs gSa&
sticky ends is/are :
(1) Eco R1 (2) Hind III (1) Eco R1 (2) Hind III
(3) Bam HI (4) All of these (3) Bam HI (4) mijksDr lHkh
119. The enzymes which are absolutely necessary for re- 119. iquZ;kstd DNA rduhdh esa ije vko';d :i ls mi;ksx
combinant DNA technology are : esa fy, tkus okys ,Utkbe gSa:
(1) Peptidases and ligases (1) Peptidases rFkk ligases
(2) Restriction endonucleases and topoisomerases (2) Restriction endonucleases rFkk topoisomerases
(3) Endonucleases and polymerases (3) Endonucleases rFkk polymerases
(4) Restriction endonucleases and ligases (4) Restriction endonucleases rFkk ligases

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120. More advancement in genetic engineering is due to : 120. vkuqokaf'kd bathfu;fjax fuEu ds dkj.k cgqr fodflr gqbZ gS
(1) Restriction endonuclease (1) Restriction endonuclease
(2) Reverse transcriptase (2) Reverse transcriptase
(3) Protease (3) Protease
(4) Zymase (4) Zymase
121. A : Agrobacterium tumefaciens is popular in genetic 121. A : Agrobacterium tumefaciens vkuq ok a f ' kd
engineering because this bacterium is associated bathfu;fjax esa yksdfiz; gS D;ksfa d ;g thok.kq vukt
with the roots of all cereal and pulse crops. rFkk nky Qlyksa dh tM+ksa ls lacaf/kr gSA
R : A gene incorporated in the bacterial chromosomal R : tc fdlh thu dks thok.kq xq.klw= thukse esa lfEefyr
genome-gets automatically transferred the crop dj fn;k tkrk gS rks og Lo;a Qly ds lkFk LFkkukUrfjr
with which the bacterium is associated. gks tkrs gS ftlls thok.kq lacaf/kr gSA
(1) If both A and R are true and R is the correct (1) ;fn A ,oa R nksuksa lR; gS rFkk R, A dk lgh
explanation of A. Li"Vhdj.k gSA
(2) If both A and R are true but R is not correct (2) ;fn A ,oa R nksuksa lR; gS] ysfdu R] A dk lgh
explanation of A. Li"Vhdj.k ugha gSA
(3) If A is true but R is false (3) ;fn A lR; gS] ysfdu R vlR; gSA
(4) If both A and R are false (4) A o R nksuksa vlR; gSA
122. The down stream processing and quality control test- 122. Down stream izØe rFkk xq.koÙkk fu;a=kd ijh{k.k r-
ing during r-DNA technology : DNA rduhdh esa :
(1) remain same for every product. (1) izR;sd mRikn gsrq leku jgrs gSa
(2) vary from product to product. (2) mRikn nj mRikn fuHkZj djrs gSa
(3) Not confirm. (3) fuf'pr ugha gksrs
(4) Mostly same but some time vary. (4) vf/kdka'kr% leku ysfdu dHkh&dHkh vleku gksrs gSa
123. Correctly matched the list-I with List-II. 123. List-I dks List-II ds lkFk lqesfyr dhft, %
List I List II List I List II
A. DNA ligase (i) Active at high temp. A. DNA ligase (i) mPp rki ij fØ;k'khy
B. Cloning sites (ii) Continuous culturing B. Cloning sites (ii) lrr lao/kZu
C. Taq-DNA polymerase (iii) First restriction endo- C. Taq-DNA polymerase (iii) izFke lhekdkjh
nuclease enzyme ,.MksU;qfDy,t ,Utkbe
D. Bioreactors (iv) Join vector and de- D. Bioreactors (iv) okgd rFkk oka f Nr
sired DNA DNA dks tksM+rk gS
E. Hind II (v) Site where foreign E. Hind II (v) LFky tgka ckgjh DNA
DNA is ligate tqM+rk gS
A B C D E A B C D E
(1) (i) (iv) (iii) (v) (ii) (1) (i) (iv) (iii) (v) (ii)
(2) (iv) (v) (iii) (ii) (i) (2) (iv) (v) (iii) (ii) (i)
(3) (iv) (v) (i) (ii) (iii) (3) (iv) (v) (i) (ii) (iii)
(4) (iv) (ii) (i) (iii) (ii) (4) (iv) (ii) (i) (iii) (ii)
124. Antibiotics mostly obtained from : 124. vf/kdka'k izfrtSfod fuEu ls izkIr gksrs gSa&
(1) Algae (2) Fungi (1) 'kSoky (2) dod
(3) Actinomycetes (4) Both (2) and (3) (3) ,fDVuksekbflVht (4) (2) rFkk (3) nksuksa
125. Biogas can be good substitute for : 125. tSo xSl fuEu dk ,d vPNk fodYi gS&
(1) Fuel wood (2) Coal (1) bZa/ku dh ydM+h (2) dks;yk
(3) Charcol (4) oil and petrol (3) pkjdksy (4) rsy rFkk isVªky
s

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126. The transformation method that uses tungsten or gold 126. :ikUrj.k izØe esa DNA ls ysfir VaxLVu ;k lksus ds
particle coated with DNA accelerated at high veloc- d.kksa dks mPp osx ls Rofjr djokuk dgykrk gS&
ity is called :
(1) Acceleration methed (1) Rofjr fof/k
(2) High velocity methed (2) mPp osx fof/k
(3) Particle gun delivery methed (3) d.k cUnwd fMfyojh fof/k
(4) RNA particle delivery methed (4) RNA d.k fMfyojh fof/k
127. Applications, like bioremediation, processes food, 127. tSo mipkj] [kk| izlaLdj.k] fpfdRlh; rFkk funku
therapeutics and diagnostics are related with : vkfn dk mi;ksx fuEu ls lacfa /kr gS&
(1) Biochemistry (2) Microbiology (1) tSojlk;u (2) lw{ethoh
(3) Biotechnology (4) Medical science (3) tSoizks|ksfxdh (4) vkS"k/kh foKku
128. Plants, bacteria, fungi and animals whose genes have 128. ikni] thok.kq] dod rFkk tUrqvksa esa thul dks rksM&
+ tksMd
+ j
been altered by manipulation are called : cnyk tkrk gS] dgykrk gS&
(1) Genetically modified organisms (1) vkuqokaf'kd :ikUrfjr tho
(2) Hybrid organisms (2) ladfjr tho
(3) Pest resistant organisms (3) ihM+d izfrjks/kh tho
(4) Insect resistant organisms (4) dhV izfrjks/kh tho
129. Golden rice is a transgenic variety of rice which con- 129. Lo.khZ; pkoy VªkaltSfud pkoyksa dh ,d fdLe gS ftlesa
tains good quantities of : fuEu vPNh ek=k esa mifLFkr gksrk gS&
(1) b-carotene (pro-vitamin-A) (1) b-dsjksfVu (izksfoVkfeu-A)
(2) a-carotene (pro-vitamin-A) (2) a-dsjksfVu (izksfoVkfeu-A)
(3) g-carotene (pro-vitamin-B) (3) g-dsjksfVu (izksfoVkfeu-B)
(4) All of the above (4) mijksDr lHkh
130. The green revolution succeeded in increasing the yield 130. gfjr Økafr eq[; :i ls fuEu ds mi;ksx ls Qlyksa dh
of crops mainly due to the use of : mit c<+kus esa lQy jgk&
I. Improved varieties of the crops. I. Qlyksa dh mUur fdLeksa ds dkj.k
II. Agro-chemicals. II. tSo&jlk;fudksa ds dkj.k
III. Better management practices. III. vPNs izca/ku rjhdksa ds dkj.k
Choose the correct option : lgh fodYi gS&
(1) I and II (2) I and III (1) I rFkk II (2) I rFkk III
(3) II and III (4) I, II and III (3) II rFkk III (4) I, II rFkk III
131. Bt toxin gene have been expressed in plant in order 131. ikniksa esa Bt toxin thu dh vfHkO;fDr fuEu ds foijhr
to provide resistance against : izfrjks/k iznku djus ds fy, mi;ksx esa yh xbZ gS&
I. Tobacco budworm and armyworm. I. rEckdw dh dfydk dhM+k rFkk lSfud dhM+k
II. Beetles. II. Hk`x
a
III. Flies and mosquitoes. III. eD[kh rFkk ePNj
Choose the correct option : lgh fodYi gS&
(1) I and II (2) I and III (1) I rFkk II (2) I rFkk III
(3) II and III (4) I, II and III (3) II rFkk III (4) I, II rFkk III

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132. Consider the following statements : 132. fn, x, dFkuksa dks if<+,&
I. Earlier, insulin was extracted from pancreas of I. igys bUlqfyu dks cfy fn;s tkus okys eosf'k;ksa rFkk
slaughtered cattle and pigs which was more ef- lqvjksa ls izkIr fd;k tkrk Fkk tksfd vkuqokaf'kd
ficient than the geneticaly engineered insulin. vfHk;kaf=d bUlqfyu ls vf/kd dq'ky FkkA
II. PCR technique is being used for the detection of II. PCR rduhd ls lafnX/k AIDS jksfx;ksa esa HIV rFkk
HIV in suspected AIDS patients and genetic mu- dSalj jksfx;ksa esa vkuqokaf'kd ifjorZu dk irk yxkus
tations in suspected cancer patients. ds fy, mi;ksx esa yh tkrh gSA
III. Cystic fibrosis, haemophilia, cancer, Parkinson's III. thu fFkjsih ls Cystic fibrosis, haemophilia, can-
etc., are treated by gene therapy. cer, Parkinson's etc., bykt fd;k tkrk gSA
Which of the statements given above are correct ? fuEu esa ls dkSuls dFku lR; gS&
(1) I and II (1) I rFkk II
(2) I and III (2) I rFkk III
(3) II and III (3) II rFkk III
(4) I, II and III (4) I, II rFkk III
133. Match the following columns : 133. lqesfyr dhft,&
Column-I Column-II Column-I Column-II
A. Bacillus thuringiensis 1. Production of chitinases A. Bacillus thuringiensis 1. dkbfVusl dk fuekZ.k
B. Rhizobium meliloti 2. Scavenging of oil spill B. Rhizobium meliloti 2. rsy ds fjlko dh lQkbZ
C. Escherichia coli 3. Incorporation of nif gene C. Escherichia coli 3. nif thu dk fuxeu
D. Pseudomonas putida 4. Production of Bt toxin D. Pseudomonas putida 4. Bt toxin dk fuekZ.k
E. Trichoderma 5. Production of human insulin E. Trichoderma 5. ekuo bUlqfyu dk fuekZ.k
A B C D E A B C D E
(1) 2 4 1 5 3 (1) 2 4 1 5 3
(2) 2 4 5 1 3 (2) 2 4 5 1 3
(3) 4 3 5 2 1 (3) 4 3 5 2 1
(4) 5 4 3 1 2 (4) 5 4 3 1 2
134. Production of a human protein in bacteria by genetic 134. thok.kqvksa esa vkuqokaf'kd vfHk;kaf=dh ds }kjk ekuo izkV
s hu
engineering is possible because : dk fuekZ.k laHko gS D;ksafd&
(1) Bacterial cell can carry out the RNA splicing (1) thok.kq dksf'kdk RNA splicing vfHkfØ;kvksa dks dj
reactions ldrk gS
(2) The human chromosome can replicate in the bac- (2) ekuo xq.klw= thok.kq dksf'kdk esa izfrÑfrdj.k dj
terial cell ldrk gS
(3) The mechanism of the gene regulation is identi- (3) ekuo rFkk thok.kqvksa esa thu fofu;eu dh fof/k
cal in humans and bacteria leku gS
(4) The genetic code is universal (4) vkuqokaf'kd dwV lkoZHkkSfed gksrs gSa
135. Genetically engineered bacteria are being employed 135. vkuqokaf'kd vfHk;kaf=dh thok.kq fuEu ds fuekZ.k esa mi;ksx
for production of : gksrk gS&
(1) Thyroxine (2) Human insulin (1) Thyroxine (2) Human insulin
(3) Cortisol (4) Epinephrine (3) Cortisol (4) Epinephrine

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136. On the basis of the Millers experiment one can say
that
(1) Organic compounds have carbon, Hydrogen and
Oxygen
(2) Simple sugar like glucose were synthesized in
the this experiment
(3) Life originated in the form amino acids
136. On the basis of the Millers experiment one can say
that
(1) Organic compounds have carbon, Hydrogen and
Oxygen
(2) Simple sugar like glucose were synthesized in
the this experiment
(3) Life originated in the form amino acids

(4) Complex organic compounds formed in primi-
tive ocean before orgine of life
137. Regarding origine of life -
(a) The first from of life could have come from pre-
existing non-living organic molecules e.g. RNA,
Proteins etc
(b) Formation of life preceded by chemical evolu-
tion
(c) Condition on earth were-low temperature,
vlocanic storms and oxidizing atmosphere
(4) Complex organic compounds formed in primi-
tive ocean before orgine of life
137. Regarding origine of life -
(a) The first from of life could have come from pre-
existing non-living organic molecules e.g. RNA,
Proteins etc
(b) Formation of life preceded by chemical evolu-
tion
(c) Condition on earth were-low temperature,
vlocanic storms and oxidizing atmosphere

(d) Life came out of decaying and rotting matter (d) Life came out of decaying and rotting matter
life straw, mud etc life straw, mud etc
How many of the above statements are correct - How many of the above statements are correct -
(1) Four (2) Three (3) Two (4) One (1) Four (2) Three (3) Two (4) One
138. How many statements are not incorrect - 138. buesa ls fdrus dFku xyr gS&
(a) Pre-historic cave art developed about 18,000 mil- (a) ekuo esa izkxSfrgkfld xqQk fp=ksa dh jpuk yxHkx
lion years ago 18,000 fefy;u o"kZ iwoZ gqbZ
(b) The geolocial history of earth closely correlates (b) i`Foh dk HkwoK
S kfud bfrgkl i`Foh ds tho oSKkfud
with the biological history of earth. bfrgkl ds lkFk fudVrk ls tqM+k gqvk gSA
(c) The UV-rays from the sun brokeup water into (c) lw;Z ls vkus okyh UV-rays esa ty dks gkbMªkstu
hydrogen and oxygen and lighter hydrogen es- rFkk vkWDlhtu esa fo[kf.Mr dj fn;k rFkk gYdh
caped H2 eqDr gksxhA
(d) According to early greek thinkers spores are the (d) iwoZ xzhd fopkjdksa dk ekuuk gS fd ^Liksj* uked bdkbZ
unit of life and were transferred to different plan- vusd xzgksa esa LFkkukUrfjr gqbZ i`Foh ftlesa ,d FkhA
ets indcluding earth
(e) The universe is almost 20 billion years old (e) czãk.M yxHkx 20 billion o"kZ iqjkuk gSA
(1) Two (2) Three (3) One (4) Four (1) nks (2) rhu (3) ,d (4) pkj
139. According to chemical evolution the condition on 139. jlk;fud mn~fodkl ds vk/kkj ij vkfne i`Foh dh D;k
primitive earth were earth were? fLFkfr jgh gksxhA
(a) High temperature (b) Volcanic stroms (a) mPPk rkieku (b) TOkkykeq[kh foLQksV
(c) Reducing atmosphere con taining CH4, NH3 etc (c) vip;h okrkoj.k ftlesa CH4, NH3 mifLFkr
(d) Low temperature (d) de rkieku
(e) Oxidizing atmosphere (e) vkWDlhdkjd okrkoj.k
(1) Only b, c, and d (2) Only a, b, and e (1) dsoy b, c rFkk d (2) dsoy a, b rFkk e
(3) Only c, d and e (4) Only a, b, and c (3) dsoy c, d rFkk e (4) dsoy a, b rFkkc

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140. The first non cellular forms of life could have origi- 140. izFke vdksf'kdh; thou :i dh mRifÙk fdrus o"kksZa iwoZ
nated years back - gqbZ&
(1) 200 Million (2) 400 Million (1) 200 Million (2) 400 Million
(3) 3 Billion (4) 30 Million (3) 3 Billion (4) 30 Million
141. Which of the following condition is not applicable for 141. feyj ds iz;ksx ds fy;s fuEu esa ls dkSulh fLFkfr vuqi;
z Dq r
Miller's experiment - ugha gS&
(1) Mixture of CH4, H2, NH3 (1) CH4, H2, NH3 dk feJ.k
(2) H2O vapours at 800ºC (2) 800ºC ij tyok"i
(3) Electric sparks in closed flask (3) can ¶ykLd esa fo|qr fujkos'k
(4) Reducing environment outside jar and oxidising (4) tkj ds ckgj fjM~;fw lax okrkoj.k ,oa tkj ds vanj
environment inside jar vkWDlhMkbftax
142. Good adaptiveness means - 142. lQy vuqdwyu dk vFkZ gS&
(1) Increase in fitness (1) fQVusl esa o`f)
(2) Move to new area (2) u, LFkku ij xeu
(3) Offspring production (3) larfr mRiknu
(4) Evolution of new characters (4) u, y{k.kksa dk mn~fodkl
143. Atmosphere of earth just before the origin of life con- 143. thou dh mRifÙk ds rqjUr igys i`Foh ds okrkoj.k esa
sisted of &&&&&&& ekStwn FkhA
(1) Water vapours, CH4, NH3 and Oxygen (1) ty ok"i, CH4, NH3 vkSj vkWDlhtu
(2) CO2, NH3 and CH4 (2) CO2, NH3 vkSj CH4
(3) CH4, NH3, H2 and water vapours (3) CH4, NH3, H2 vkSj ty ok"i
(4) CH4, 03, O2 and water vapours (4) CH4, O3, O2 vkSj ty ok"i
144. Spontaneous generation theory of origin of life was dis- 144. thou dh mRifÙk ds Lor% tuu okn (Spontaneous
proved through experiment involving swan-neck flasks generation theory) dk [k.Mu] gal xzhokdkj ¶YkLd dks
by lfEefyr djds fd;s x;s ç;ksx }kjk&&&&&us fd;kA
(1) Van Helmont (2) Reddi (1) okWu gsYekW.V (2) jsM~Mh
(3) Louis Pasteur (4) Spallanzani (3) yqbl ik'pj (4) LIysta kuh
145. The scientist associated with formulation of modern 145. ^thou dh mRifÙk ds vk/kqfud fl)kUr ds çfriknu ls
theory of origin of life and having written ‘Origin of Life’ lEc) vkSj ^vksfjftu vkWQ ykbQ dks fy[kus okys oSKkfud
was Fks
(1) Oparin (2) Huxley (1) vksifs ju (2) gDlys
(3) Lamarck (4) Darwin (3) ySekdZ (4) MkfoZu
146. Organic compounds first evolved on earth and required 146. ^dkcZfud ;ksfxd tks i`Foh ij loZizFke mRié gq, vkSj
for origin of life were thou dh mRifÙk ds fy, vko';d Fks& &
(1) Urea and amino acids (1) ;wfj;k vkSj vehuks vEy
(2) Proteins and nucleic acids (2) çksVhUl vkSj U;wfDyd vEy
(3) Proteins and amino acids (3) çksVhu vkSj vehuks vEy
(4) Urea and nucleic acid (4) ;wfj;k vkSj U;wfDyd vEy
147. Possible early source of energy was 147. ÅtkZ dk çkjfEHkd laHko L=ksr Fkk &
(1) Chlorophyll (2) CO2 (1) DyksjksfQy (2) CO2
(3) UV radiations and lightning (3) UV fofdj.k vkSj fctyh
(4) Green plants (4) gjs ikS/ks
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148. Process of evolution is 148. fodkl dh çfØ;k gS &
(1) Discontinuous (1) fojy
(2) Discontinuous in the past but continuous presently (2) iqjkus le; esa fojy ijUrq orZeku esa vfojy
(3) Continuous in the past but discontinuous presently (3) iqjkus le; esa fojy ijUrq orZeku esa vfojy
(4) Continuous (4) vfojy
149. Which one of the following experiments suggests that 149. fuEu esa ls dkSulk iz;ksx ;g n'kkZrk gS fd ljyre tho
simplest living organisms could not have originated vtSfod inkFkks± ls Lor mRiUu ugha gks ldrs gSa%
spontaneously from non-living matter?
(1) Larvae could appear in decaying organic matter. (1) ykokZ lM+s&xys dkcZfud inkFkks± esa fn[k ldrk gS
(2) Microbes can appear on bread kept at a moist (2) czsM dks vkæZrk okyh txg ij j[kus ij ml ij lw{etho
place. fn[k ldrs gSa
(3) Microbes appear on unsterilized organic matter. (3) lw{etho vuLVykZbTM dkcZfud inkFkks± ij fn[krs gSa
(4) Meat was not spoiled, when heated and kept sealed (4) ekal dks xeZ djds can rZu esa j[kus ij og [kjkc ugha gqvk
in a vessel.
150. According to one of the most widely accepted theo- 150. ,d loZekU; fl)kar ds vuqlkj] thou dh mRifr ds igys
ries, earth's atmosphere before origin of life was i`Foh dk okrkoj.k Fkk%
(1) oxidizing (1) vkWDlhdkj
(2) oxidizing along with H2 (2) H2 ds lkFk vkWDlhdkjd
(3) reducing with O2 in small amount (3) O2 dh de ek=k ds lkFk vipk;d
(4) reducing with oxygen absent in O2 form. (4) O2 ds :i esa vkWDlhtu dh vuqifLFkfr ds lkFk vipk;d
151. From the point of view of early chemical evolution that 151. izFker% jklk;fud fodkl dh ifjdYiuk ds vuqljk] ftlls
preceded the origin of life on earth, the most important i`Foh ij thou dh mRifr gqbZ] lcls egRoiw.kZ fufeZr ljy
simple organic molecules formed were dkcZfud v.kq Fks%
(1) sugars and amino acids (1) 'kdZjk o vehuks vEy
(2) glycerol and fatty acids (2) fXyljkWy o olh; vEy
(3) purines and pyrimidines (3) I;wjhUl o fijhfeMhUl
(4) all of these. (4) lHkh
152. Match Column-I with Column-ll and select the correct 152. Column-I dks Column-ll ls esy djk;sa vkSj uhps fn, x, dksM
option from the codes given below. ls lgh fodYi pqusa%
Column-I Column-ll Column-I Column-ll
A. Francesco Redi (i) Theory of chemical A. Francesco Redi (i) thou ds jklk;fud fodkl
evolution of life dk fl)kar
B. L. Pasteur (ii) Disproval of B. L. Pasteur (ii) Lor% tuuokn dks vlR;
spontaneous generation fl) djuk
C. Richter (iii) Swan necked flask C. Richter (iii) gal dh xnZu tSls ¶ykLd
experiment dk iz;ksx
D. Oparin (iv) Mutation D. Oparin (iv) mRifjorZu
(v) Panspermia (v) isuLifeZ;k
(1) A-(v), B-(i), C-(iv), D-(ii) (1) A-(v), B-(i), C-(iv), D-(ii)
(2) A-(ii), B-(iii), C-(v), D-(i) (2) A-(ii), B-(iii), C-(v), D-(i)
(3) A-(v), B-(iv), C-(ii), D-(i) (3) A-(v), B-(iv), C-(ii), D-(i)
(4) A-(i), B-(ii), C-(iii), D-(iv) (4) A-(i), B-(ii), C-(iii), D-(iv)
153. Who proposed that the first form of life could have come 153. fdlus igyh ckj ;g izfrikfnr fd;k fd thou dk igyk :i
pre existing non-living organic molecules? igys ls mifLFkr vtSfod dkcZfud v.kqvksa ls vfLrRo esa vk;k%
(1) S.L. Miler (2) Oparin and Haldane (1) S.L. Miler (2) Oparin and Haldane
(3) Charles Darwin (4) Alfred Wallace (3) Charles Darwin (4) Alfred Wallace
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154. Consider following statements regarding microsphere, 154. ekbØksLQh;lZ ls lacaf/kr fuEu dFkuksa dks ns[ksa%
i. They were spherical in shape and 1-2 mm in diam- i. ;s vkdkj esa xksy Fks vkSj budk O;kl 1-2 mm Fkk
eter
ii. They had concentric double-layered boundaries ii. buesa
nks ladsUæh ijrksa okyk ?ksjk Fkk
iii. they provided partial isolation to contents inside it, iii. buesa mifLFkr vo;o vkaf'kd :i ls ckgjh okrkoj.k ls
from external environment i`Fkd Fks
iv. They had catalytic activity iv. muesa mRizsjd fØ;k,a gksrh Fkha
Which of the above statements are incorrect? dkSulk dFku xyr gS%
(1) i only (2) ii only (1) dsoy i (2) dsoy ii
(3) iv only (4) none of the above (3) dsoy iv (4) buesa ls dksbZ ugha
155. The prebiotic atmosphere of the earth was of a reduang 155. i`Foh dk iwoZ tSfod ok;qeM a y vipk;d izÑfr dk FkkA ;g
nature. It was transformed into an oxidizing atmosphere orZeku esa vkWDlhdkjd okrkoj.k esa fuEu dh mRifr ds dkj.k
of present day due to the emergence of ifjofrZr gqvk%
(1) cyanobacteria (1) cyanobacteria
(2) angiosperms (2) angiosperms
(3) photosynthetic bacteria (3) izdk'k la'ys"kh bacteria
(4) eukaryotic algae. (4) eukaryotic algae.
156. The sequence of origin of life may be : 156. thou dh mRifr dk Øe gks ldrk gS%
(1) Inorganic materials ® Organic materials ® colloi- (1) vdkcZfud inkFkZ ® dkcZfud inkFkZ ® dksykWbMy lewg
dal aggregate ® Eobiont ® Cell ® bvksck;ksUV ® dksf'kdk
(2) Organic materials ® Inorganic materials ® colloi- (2) dkcZfud inkFkZ ® vdkcZfud inkFkZ® dksykWbMy lewg ®
dal aggregate ® Eobiont ® Cell bvksck;ksUV ® dksf'kdk
(3) Inorganic materials ® Organic materials ® Eobiont (3) vdkcZfud inkFkZ® dkcZfud inkFkZ ® bvksck;ksUV ® dksf'kdk
® Cell® colloidal aggregate ® dksykWbMy lewg
(4) Organic materials ® Inorganic materials ® Eobiont (4) dkcZfud inkFkZ ® vdkcZfud inkFkZ® bvksck;ksUV ®
® Cell® colloidal aggregate dksf'kdk ® dksykWbMy lewg
157. The following are some major events in the early his- 157. thou ds vkajfHkd bfrgkl dh dqN eq[; ?kVuk,a fuEu gSa%
tory of life :
P.First heterotrophic prokaryotes P. izFke fo"keiks"kh izkd
s sfj;ksV~l
Q. First genes Q. izFke thu
R. First eukaryotes R. izFke ;wdsfj;ksVl ~
S. First autotrophic prokaryotes S. izFke Loiks"kh izksdsfj;ksV~l
T. First anirnals T. izFke tUrq
Which option below places these events in the cor- fn, x, fuEu Øeksa esa ls dkSulk Øe mijksDr ds fy, lgh gS%
rect order :
(1) PQSRT (2) QSPTR (3) QPSRT (4) QSPRT (1) PQSRT (2) QSPTR (3) QPSRT (4) QSPRT
158. The first life originated 158. dizFke thou mRiUu gqvk
(1) on land (2) in air (1) i`Foh ij (2) ok;q esa
(3) in water (4) all of these. (3) ty esa (4) mijksDr lHkh
159. Abiogenesis theory of origin supports 159. mRifr dk Lor% tuuokn leFkZu djrk gS
(1) spontaneous generation (1) Lor% mRifr
(2) origin of life from blue-green algae (2) uhyk&gjh 'kSoky ls thou dh mRifr
(3) origin of life is due to pre-existing organisms (3) iwoZ^&vfLrRo okys thoksa ds dkj.k thou dh mRifr
(4) organic evolution is due to chemical reactions. (4) jklk;fud fØ;kvksa ds dkj.k dkcZfud fodkl

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160. Match the scientists in Column-I with their related dis- 160. Column-I ds oSKkfudksa dks column-ll esa nh xbZ mudh [kkstksa
coveries in column-ll and select the correct option from ds lkFk lqefs yr dhft, vkSj uhps fn, x, dksM ls lgh fodYi
the codes given below. pqfu, %
Column I Column II Column I Column II
A. Kary Mullis i. Father of genetic A. Kary Mullis i. vuqokaf'kd vfHk;kaf=dh ds
engineering firk
B. Paul Berg ii. Nobel prize for the B. Paul Berg ii. izfrca/ku ,.MksU;wfDy;st dh
discovery of restriction [kkst ds fy, ukscy iqjLdkj
endonucleases
C. Stanley Cohen and iii. Developed polymerase C. Stanley Cohen and iii. ikWyhejst psu vfHkfØ;k
Herbert Boyer chain reaction Herbert Boyer dks fodflr djuk
D. Arber, Smith and iv. Isolated an antibiotic D. Arber, Smith and iv. lkYeksuy
s k Vk;QhE;wfj;e
Nathan resistant gene from a Nathan thok.kq ds IykfTeM ls
plasmid of the bacterium ,UVhck;ksfVd izfrjks/kh thu dks
Salmonella typhimurium i`Fkd fd;k
(1) A-(iii), B-(i), C-(iv), D-(ii) (1) A-(iii), B-(i), C-(iv), D-(ii)
(2) A-(iii), B-(iv), C-(i), D-(ii) (2) A-(iii), B-(iv), C-(i), D-(ii)
(3) A-(iv), B-(ii), C-(iii), D-(i) (3) A-(iv), B-(ii), C-(iii), D-(i)
(4) A-(i), B-(iii), C-(iv), D-(ii) (4) A-(i), B-(iii), C-(iv), D-(ii)
161. Plasmid used to construct the first recombinant DNA 161. izFke iqu;ksZxt DNA cukus ds fy, fuEuj esa ls fdl cSDVhfj;k
was isolated from which bacterium species? ls i`FkDÑr IykfTeM dk mi;ksx fd;k x;k Fkk%
(1) Escherichia coli (1) Escherichia coli
(2) Salmonella typhimurium (2) Salmonella typhimurium
(3) Agrobacterium tumefaciens (3) Agrobacterium tumefaciens
(4) Thermus aquaticus (4) Thermus aquaticus
162. The term 'molecular scissors' refers to 162. 'vkf.od dSaph' 'kcn ls vfHkizk; gS%
(1) recombinant DNA (1) iqu;ksx
Z t DNA
(2) restriction enzymes (2) izfrca/ku ,UtkbEl
(3) Taq polymerase (3) Vsd ikWyhejst
(4) palindromic nucleotide sequences (4) isfyUMªksfed U;wfDy;ksVkbM vuqØe
163. The term 'recombinant DNA' refers to 163. 'iqu;ksZxt DNA' 'kCnls vfHkizk; gS%
(1) DNA of the host cell (1) gksLV dksf'kdk dk DNA
(2) DNA with a piece of foreign DNA (2) ckg~; DNA ds [k.M dks fufgr j[kus okyk DNA
(3) DNA with selectable marker (3) oj.k;qDr fpUgd okyk DNA
(4) DNA with more than one recognition sites. (4) ,d ls vf/kd igpku LFkyksa okyk DNA
164. Which of the following statements is not correct? 164. fuEu esa ls dkSulk dFku xyr gS%
(1) Recombinant technologies are used to produce (1) okafNr izksVhUl ds mRiknu ds fy, iqu;ksZxt rduhdksa dk
desirable proteins. mi;ksx fd;k tkrk gS
(2) Agrobacterium is a genus of bacteria that causes (2) ,xzkscSDVhfj;e thok.kqvksa dk ,d oa'k gS] tks ikS/ks esa V~;ew j
tumours in plants. mRiUu djrk gS
(3) Log phase does not show any significant increase (3) ykWx izkoLFkk esa dksf'kdkvksa dh la[;k esa dksbZ egRoiw.kZ c<+r
in the number of the cells whereas lag phase shows ugha gkrh] tcfd ysx izkoLFkk esda dksf'kdkvksa esa rhoz xq.ku
rapid multiplication of the cells. gksrk gS
(4) Dolly, a sheep was the first animal to be cloned in (4) MkWyh] ,d HksM+] izFke tUrq Dyksu gS] ftls 1997 esa Dyksu
1997 fd;k x;k Fkk

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165. Which of the following are parts of biotechnology? 165. fuEu esa ls dkSu tSo&izkS|ksfxdh ds Hkkx gSa%
(1) In vitro ferilization (1) bu&foVªks fu"kspu
(ii) Synthesis of a gene (ii) ,d thu dk la'ys"k.k
(iii) Correcting a defective gene (iii) foÑr thu dks lq/kkjuk
(iv) Developing a DNA vaccine (iv) DNA Vhds dks fodflr djuk
(1) i & ii (2) ii & iii (1) i & ii (2) ii & iii
(3) iii & iv (4) i, ii, iii & iv (3) iii & iv (4) i, ii, iii & iv
166. Genetic engineering is possible because 166. vuqokaf'kd vfHk;kaf=dh laHko gks ikbZ] D;ksafd%
(1) we can cut DNA at specific sites by restriction (1) jsfLVªD'ku ,.MksU;wfDy;st }kjk ge DNA dks fof'k"V LFkyksa
endonucleases ls dkV ldrs gSa
(2) restriction endonucleases purified from virus can (2) fo"kk.kq ls izkIr 'kq) jsfLVªD'ku ,.MksU;wwfDy;st dk mi;ksx
be used in bacteria cSDVhfj;k esa fd;k tk ldrk gS
(3) the phenomenon of transduction in bacteria is well (3) cSDVhfj;k esa gksus okyh ikjØe.k izØ;k dks vPNh rjg ls
understood le>k tk pqdk gS
(4) we can see DNA by electron microscope. (4) ge bysDVªkWu ekbØksLdksi }kjk DNA dks ns[k ldrs gSa
167. A restriction endonuclease breaks bonds between the 167. jsfLVªD'ku ,.MksU;wfD;st fduds e/; cus ca/kksa dks rksM+rs gSa%
(1) base pairs of a DNA molecule as elution. (1) DNA v.kq ds {kkj ;qXeksa
(2) base pairs of a DNA-RNA hybrid molecule (2) DNA-RNA gkbfczM v.kq ds {kkj ;qXeksa
(3) sugar and phosphate components of a nucleic acid (3) U;wfDyd vEy v.kq ds 'kdZjk vkSj QkLQsV ?kVdksa
molecule (4) DNA v.kq ds ,DlkWu o bUVªkWu
(4) exons and introns of a DNA molecule.
168. ldentify the palindromic sequence in the following. 168. fn, x, fodYiksa esa ls isfyUMªkse Øe dks igpkfu,%

GAATTC GGATCC GAATTC GGATCC

(1) (2) (1) (2)
CTTUUG CCTAGG CTTUUG CCTAGG

CCTGG CGATA CCTGG CGATA

(3) (4) (3) (4)
GGACC GCTAA GGACC GCTAA

169. If a recombinant DNA bearing gene for resistance to
antibiotic ampicillin is transferred to E.coli cells, the
host cells become transformed into ampicillin resis-
tant cells. If such bacteria are transferred on agar plates
containing ampicillin, only transformants will grow and
the untransformed recipient cells will die. The ampicil-
lin resistant gene in this case is called as
(1) selectable marker (2) recombinant protein
(3) cloning site (4) chemical scalpels.
170. Which of the following tools of recombinant DNA tech-
nology is incorrectly paired with its use?
(1) Eco RI - Production of sticky ends
(2) DNA ligase - Multiplication of rDNA molecules
(3) DNA polymerase - In polymerase chain reaction to
amplify sections of DNA
(4) Selectable marker - Identification of transformants
169. ;fn ,Eihflfyu ,UVhck;ksfVd izfrjks/kh thu okys iqu;ksZxt thu
dks E.coli dksf'kdkvksa esa LFkkukUrfjr fd;k tk,] rks ijiks"kh dksf'kdk
,Eihflfyu izfrjks/kh dksf'kdkvksa esa cny tkrh gSA ;fn bu cSDVhfj;k
dks ,Eihflfyu okys vxj ek/;e esa LFkkukUrfjr fd;k tk, rks
dsoy :ikarfjr dksf'kdk,a gh ml ek/;e esa o`f) djrh gSa vkSj
v:ikarfjr xzkgh dksf'kdkvksa dh e`R;q gks tkrh gSA bl voLFkk esa
,Eihflfyu izfrjks/kh thu dks dgax sa s%
(1) oj.k;ksX; fpUgd (2) iqu;ksZxt izkV
s hu
(3) Dyksfuax LFky (4) jklk;fud Ldsyisy
170. fuEu esa ls fjdkWEchusUV DNA rduhd dk dkSu lk lk/ku mlds
dk;Z ds lkFk lqefs yr ugha gS\
(1) Eco RI - fpidus okys fljs mRiUu djuk
(2) DNA ligase - rDNA v.kqvksa dk cgqxq.ku
(3) DNA ikWyhejst - ikWyhejst psu vfHkfØ;kvksa esa DNA
[k.Mksa dk izo/kZu
(4) oj.k;ksX; fpUgd - :ikUrfjr dksf'kdk dh igpku

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171. Which one of the following characteristics is gernerally 171. ,d Dyksfuax okgd ds fy, fuEu esa ls dkSu ls y{k.k dks
preferred for a cloning vector? izkFkfedrk ugha nh tkrh gS%
(1) An antibiotic origin of replication (1) ,d izfrÑfr;u mn~xe LFky
(2) An antibiotic resistance marker (2) ,d ,UVhck;ksfVd izfrjks/kh ekdZj
(3) Multiple restrictionsites (3) vusd izfrca/ku LFky
(4) A high copy number (4) mPp izfr la[;k
172. DNA cannot pass through a cell membrane as 172. DNA dksf'kdk f>Yyh dks ikj ugha dj ldrk D;ksfa d%
(1) It is too bit to cross the membrane (1) f>Yyh dks ikj djus ds fy, bldk vkdkj cgqr cM+k gS
(2) it is a hydrophilic molecule (2) ;g ,d tyLusgh v.kq gS
(3) membrane does not ahve specific proteins to facili- (3) f>Yyh esa blds ifjogu ds fy, fof'k"V izksVhu v.kq ugha
tate the transport ik;s tkrs gSa
(4) none of the above (4) buesa ls dksbZ ugha
173. The letter 'R' in Eco R I is derived from 173. Eco R I esa R fdlls O;qRiUu gksrk gS
(1) the name of genus (2) the name of strain (1) oa'k dk uke (2) izHksn dk uke
(3) the name of species (4) the term 'restriction (3) tkfr dk uke (4) jsfLVªD'ku 'kCn
174. Which of the following is not used to transfer the re- 174. fjdkWEchusUV DNA dks gksLV dksf'kdk esa LFkkukUrfjr djus ds
combinant DNA into the host? fy, fuEu esa ls fdl fof/k dk mi;ksx ugha fd;k tkrk gS%
(1) Micro-injection method (1) lw{e&vUr% {ksi.k fof/k
(2) Gene gun method (2) thu&xu fo/kh
(3) Bioreactors (3) ck;ksfj,DVlZ
(4) Disarmed pathogen vectors (4) Hkqtkjfgr jksxdkjd okgd
175. Micro-injection is a method used to 175. lw{e vUr% {ksi.k fof/k dk mi;ksx fd;k tkrk gS%
(1) produce sticky ends of DNA (1) DNA esa fpidus okys fljs mRiUu djus gsrq
(2) provide protection against pathogen (2) jksx tudksa ls lqj{kk iznku djus ds fy,
(3) purify the DNA (3) DNA dks 'kq) djus ds fy,
(4) inject recombinant DNA into the nucleus of an ani- (4) tUrq dksf'kdk ds dsUæd esa fjdkEchusUV DNA dks bUtsDV
mal cell. djus ds fy,
176. During insertional inactivation, the presence of a chro- 176. fuos'kh fuf"Ø;rk esa] jax mRiUu djus okyk lClVªsV rc uhys
mogenic substrate gives blue coloured colonies if the jax dh dkWyksuh nsrk gS tc cSDVhfj;k ds IykfTeM esa bUlVZ ugha
plasmid in the bacteria does not have an insert. The gksrk gSA uhyk jax bl ,Utkbe }kjk mRiUu fd;k tkrk gS%
blue colour is produced by the enzyme
(1) a - glucosidase (1) a - glucosidase
(2) restriction endonuclease (2) restriction endonuclease
(c) b-galactosidase (c) b-galactosidase
(4) Taq polymerase. (4) Taq polymerase.
177. Which of the following statements is correct for mo- 177. vkf.od izksCl ds fy, fuEu esa ls dkSulk dFku lgh gS%
lecular probes?
(1) Used to detect the complementary sequence in (1) U;wfDyd vEy ds uewus esa iwjd Øeksa dks irk djus esa
nucleic acid samples bldk mi;ksx fd;k tkrk gS
(2) Used to introduce recombinant DNA into an animal (2) ,d tUrq dksf'kdk esa fjdkWChusUV DNA dks Mkyus ds fy,
cell bldk mi;ksx fd;k tkrk gS
(3) Used to cut DNA at random positions (3) DNA dks vfuf'pr LFkyksa ij dkVus ds fy, bldk mi;ksx
(4) Facilitate the multiplication of DNA fd;k tkrk gS
(4) DNA ds cgqxq.ku esa lgk;rk djrk gS

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178. The sticky ends of a fragmented DNA molecule are 178. [kf.Mr DNA ds fpidus okys fljs cus gksrs gSa%
made of (1) dSfYl;e yo.k ls (2) ,.MksU;wfDy,t ,Utkbe ls
(1) calcium salts (2) endonuclease enzyme (3) v;qfXer {kkjksa ls (4) esfFky lewgksa ls
(3) unpaired bases (4) methyl groups. 179. Column-I esa fn, x, 'kCnksa dks Column-ll esa nh xbZ mudh
179. Match the terms given in Column-I with their defini- ifjHkk"kkvksa ds lkFk feykb, vkSj uhps fn, x, dksM ls lgh
tions in Column-ll and select the correct anwser from mÙkj pqfu, %
codes given below. Column-I Column-ll
Column-I Column-ll A. :ikUrj.k (i) jsfLVªD'ku ,Utkbe }kjk
A. Transformation (i) Sequences cut by dkVs x, fljs
restriction enzymes B. igpku , (ii) og izfØ;k ftlds }kj DNA
B. Recognition , (ii) Process by which DNA [k.Mksa dks vkdkj ds vk/kkj ij
sequences fragments are i`FkDÑr fd;k tkrk gS
separated based on their
size
C. Gel electrophoresis (iii) Plasmid DNA that has C. Gel electrophoresis (iii) ekuo DNA ;qDr IykfTeM
incorporated human DNA DNA
D. Recombinant DNA (iv) Process by which bacteria D. fjdkEchusaV DNA (iv) og izfØ;k ftlds }kjk
take up pieces of DNA from cSDVhfj;k okrkjoj.k ls DNA
the environment ds VqdM+ksa dks xzg.k djrk gS
(1) A-(iii), B-(i), C-(ii), D-(iv) (1) A-(iii), B-(i), C-(ii), D-(iv)
(2) A-(iv), B-(i), C-(ii), D-(iii) (2) A-(iv), B-(i), C-(ii), D-(iii)
(3) A-(i), B-(ii), C-(iii), D-(iv) (3) A-(i), B-(ii), C-(iii), D-(iv)
(4) A-(ii), B-(iii), C-(iv), D-(i) (4) A-(ii), B-(iii), C-(iv), D-(i)
180. A correct pair of characteristics of molecular probe is 180. vkf.od izksc ds y{k.kksa dk lgh tksM+k gS%
(A) very long molecule (B) double stranded (A) cgqr yEck v.kq (B) f}jTtqdh
(C) single stranded DNA or RNA (C) flaxy LVªUs MsM DNA ;k RNA
(D) complementary to part of desired gene (D) okafNr thu ds lEiwjd
(1) a & B (2) B & C (3) C & D (4) A & D. (1) a & B (2) B & C (3) C & D (4) A & D.

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