6.14.

The maximum growth yield coefficient for Bacillus subtilis growing on

methanol is 0.4 gX/gS. The heat of combustion of cells is 21 kJ/g cells and for
substrate it is 7.3 kcal/g. Determine the metabolic heat generated by the cells per
unit mass of methanol consumption.

Answer:

𝑔𝑋
YX = 0.4
S 𝑔𝑆

𝑘𝐽
∆HC = 21 𝑔 𝑐𝑒𝑙𝑙𝑠

𝑘𝑐𝑎𝑙
∆HS = 7.3 𝑔

∆𝐻𝑆 1
= ∆𝐻𝐶 +
YX 𝑌𝐻
S

𝑘𝑐𝑎𝑙 4.184 𝑘𝐽
7.3 𝑔 × 1 𝑘𝑐𝑎𝑙 𝑘𝐽 1
= 21 +
𝑔𝑋 𝑔 𝑐𝑒𝑙𝑙𝑠 𝑌𝐻
0.4 𝑔𝑆

𝑘𝐽
30.5432 𝑘𝐽 1
𝑔
= 21 +
𝑔𝑋 𝑔 𝑐𝑒𝑙𝑙𝑠 𝑌𝐻
0.4 𝑔𝑆

𝑘𝐽 𝑔𝑋 𝑘𝐽 1
30.5432 = 0.4 (21 + )
𝑔 𝑔𝑆 𝑔 𝑐𝑒𝑙𝑙𝑠 𝑌𝐻

𝑘𝐽 𝑘𝐽 𝑔𝑋 1
30.5432 = 8.4 + (0.4 × )
𝑔 𝑔 𝑔𝑆 𝑌𝐻

𝑘𝐽 𝑘𝐽 𝑔𝑋 1
30.5432 − 8.4 = 0.4 ×
𝑔 𝑔 𝑔𝑆 𝑌𝐻

𝑘𝐽 𝑔𝑋 1
22.1432 = 0.4 ×
𝑔 𝑔𝑆 𝑌𝐻

1 𝑘𝐽
= 55.358
𝑌𝐻 𝑔 𝑐𝑒𝑙𝑙𝑠
Solving the metabolic heat generated by the cells per unit mass of methanol
consumption:

1 𝑘𝐽
= 55.358
𝑌𝐻 𝑔 𝑐𝑒𝑙𝑙𝑠

𝑘𝐽 𝑔𝑋
55.358 × 0.4
𝑔 𝑐𝑒𝑙𝑙𝑠 𝑔𝑆

Ans= 𝟐𝟐. 𝟏𝟒𝟑 𝒌𝑱⁄𝒈 𝒔𝒖𝒃𝒔𝒕𝒓𝒂𝒕𝒆