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  • Gráficas Zymomonas

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    Andrea Chavez
    11 views10 pages
    The document contains data from an experiment measuring glucose, ethanol, glycerol, lactate, and acetate levels over time under different conditions: nitrogen gas (N2), 1% dissolved oxygen (DO 1%), 10% dissolved oxygen (DO 10%), and in a flask without control of oxygen levels. The levels of each metabolite changed over the course of 48 hours, with glucose generally decreasing over time while ethanol, glycerol, lactate and acetate increased at different rates depending on the oxygen conditions.

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    The document contains data from an experiment measuring glucose, ethanol, glycerol, lactate, and acetate levels over time under different conditions: nitrogen gas (N2), 1% dissolved oxygen (DO 1%), 10% dissolved oxygen (DO 10%), and in a flask without control of oxygen levels. The levels of each metabolite changed over the course of 48 hours, with glucose generally decreasing over time while ethanol, glycerol, lactate and acetate increased at different rates depending on the oxygen conditions.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    11 views10 pages

    Gráficas Zymomonas

    Uploaded by

    Andrea Chavez
    The document contains data from an experiment measuring glucose, ethanol, glycerol, lactate, and acetate levels over time under different conditions: nitrogen gas (N2), 1% dissolved oxygen (DO 1%), 10% dissolved oxygen (DO 10%), and in a flask without control of oxygen levels. The levels of each metabolite changed over the course of 48 hours, with glucose generally decreasing over time while ethanol, glycerol, lactate and acetate increased at different rates depending on the oxygen conditions.

    Copyright:

    © All Rights Reserved
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 10

    Glucosa

    HORAS g/L
    tiempo GLUCOSA N2 GLUCOSA DO 1% GLOUCOSA DO 10% GLUCOSA FLASK
    0 100 100 100 100
    22 97 0.1 60 40
    30 82 0.1 45 0.1
    48 0 0 34 0

    GLUCOSA g/L
    120

    100

    80
    Gucosa g/L

    60

    40

    20

    0
    0 10 20 30 40 50

    TIEMPO (HORAS)

    GLUCOSA N2 GLUCOSA DO 1% GLUCOSA DO 10% GLUCOSA FLASK


    OD 600nm 1% OD 600nm DO 10% OD 600nm(flask) OD600nm N2
    g/L
    OD 600 nm 1% OD 600NM DO10% OD 600NM (FLASK) OD600nm N2
    0.1 0.1 0.1 0.1
    5.34 2 4.7 0.3
    4.4 2.5 5.7 1.3
    4.3 1.65 6.3 3.6

    10

    6
    OD 600nm Value

    0
    40 50 60

    DO 10% GLUCOSA FLASK


    m(flask) OD600nm N2
    ETHANOL
    HORAS g/L
    tiempo ETHANOL N2 ETHANOL DO 1% ETHANOL DO 10% ETHANOL FLASK
    0 0 0 0 0
    22 2 19 4 18
    30 5 17 5 30
    48 42 15 5 30
    ANOL
    g/L
    2,3-BDO N2 2,3-BDO DO 1% 2,3-BDO DO 10% 2,3-BDO Flask
    0 0 0 0
    35 3 9 40
    34 4 10 50
    33 4 10 48

    60

    ETHANOL N2
    50
    2,3-BDO N2

    ETHANOL DO
    40 1%
    Ethanol (g/L)

    ETHANOL DO
    30 10%

    ETHANOL
    FLASK
    20
    2,3-BDO Flask

    2,3-BDO DO
    10 10%

    2,3-BDO DO 1%

    0
    0 10 20 30 40 50 60
    TíME (H)
    ETHANOL N2

    2,3-BDO N2

    ETHANOL DO
    1%

    ETHANOL DO
    10%

    ETHANOL
    FLASK

    2,3-BDO Flask

    2,3-BDO DO
    10%

    2,3-BDO DO 1%

    60
    LACTATE Y ACETATE
    HORAS g/L
    Tiempo Glicerol(N2) Glicerol(DO01%) Glicerol(DO10%) Glycerol (Flask)
    0 0.1 0.1 0.1 0.1
    22 0.2 0.2 0.4 1.5
    30 0.2 0.4 0.6 3.1
    48 0.2 0.6 0.6 3.1

    Lactate y Acetate (g/L)


    5

    0
    0 10 20 30 40 50
    Time (h)
    ATE Y ACETATE
    g/L
    Acetate (N2) Acetate (DO1%) Acetate (DO10%) Acetate Flask
    0 0 0.1 0
    0 5.8 3.8 0
    0 6.4 4.8 1
    0.1 6.8 5.2 1.4

    Glicerol(N2)
    Glicerol(DO01%)
    Glicerol(DO10%)
    Glycerol (Flask)
    Acetate (N2)
    Acetate (DO1%)
    Acetate (DO10%)
    Acetate Flask

    20 30 40 50 60
    Time (h)
    LACTATE Y ACETATE
    HORAS g/L
    Tiempo Lactate (N2) Lactate (DO1%) Lactate (DO10%) Lactate (Flask)
    0 0.21 0.2 0.2 0.2
    22 0.22 0.6 0.57 0.2
    30 0.19 0.68 0.62 0.24
    48 0.57 0.8 0.86 0.18

    1.2

    Lactate y Acetate (g/L)


    0.8

    0.6

    0.4

    0.2

    0
    0 10 20 30 40 50
    Time (h)
    ATE Y ACETATE
    g/L
    Acetate (N2) Acetate (DO1%) Acetate (DO10%) Acetate Flask
    0.25 0.25 0.25 0.25
    0.25 0.35 0.42 0.18
    0.2 0.62 0.58 0.2
    0.3 1 0.8 0.28

    Lactate (N2)
    Lactate (DO1%)
    Lactate (DO10%)
    Lactate (Flask)
    Acetate (N2)
    Acetate (DO1%)
    Acetate (DO10%)
    Acetate Flask

    20 30 40 50 60
    Time (h)

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