UNIVERSITY OF ZIMBABWE

2018 May/June Examinations

Science
Faculty:...................................................................................................................................
Biochemistry
Department:............................................................................................................................
HBC201: Practical Techniques in Biochemistry 2

Paper code and Title:............................................................................................................

2 hours
Duration:.................................................................................................................................
Ms B. Jani
Examiner:................................................................................................................................

Authorized Materials:

INSTRUCTIONS:

1. This paper contains 6 Questions

2. Answer 4 questions
3. Start each question on a new page
4. This question paper comprises 2 printed pages

NB: DO NOT TURN OVER THE QUESTION PAPER OR COMMENCE WRITING UNTIL
INSTRUCTED TO DO SO.
HBC201 PAGE ONE

1.
(a) Outline the following immunological methods used in biomedical analysis:

(i) immunofluorescence [5 marks]

(ii) enzyme-linked immunosorbent assay [5 marks]

(b) Describe how a fraction of lymphocytes can be obtained from blood and list the uses.
[15 marks]

2.
(a) Using a suitable example, briefly describe the basis of separation of biomolecules by
affinity chromatography. [9 marks]

(b) Discuss the limitations of using affinity chromatography in the purification of

biomolecules. [16 marks]

3. Explain why:

(a) Anion exchange chromatography is carried out using a buffer whose pH is above the pI
of the protein. [13 marks]

(b) Two 30 kDa proteins, one globular and another fibrous, can still be separated from each
other by gel filtration chromatography. [12 marks]

4.
(a) Discuss wall coated open tubular columns (WCOT) and support coated open tubular
columns (S COT) used for gas chromatography. [13 marks]

(b) Write notes on 2D gel electrophoresis. [12 marks]

5. Discuss how you would separate a mixture of monosaccharides using high performance
liquid chromatography (HPLC). [25 marks]

PLEASE TURN TO THE NEXT PAGE

HBC201 PAGE TWO

6. Briefly describe the following:

(a) agarose gel electrophoresis [8 marks]

(b) SDS-PAGE [8 marks]

(c) nucleic acid sequencing gells [9 marks]

END OF QUESTION PAPER