Register Number

SATHYABAMA UNIVERSITY
(Established under section 3 of UGC Act,1956)
Course & Branch :M.Tech - BTE/W-BTE Max. Marks :80
Title of the Paper :Fermentation and Downstream Processing
Sub. Code :SCHX5024 Time : 3 Hours
Date :03/06/2011 Session :FN
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PART - A (6 x 5 = 30)
Answer ALL the Questions
1. Discuss the commercially important fermentations.
2. Write the formula for substrate utilization rate and product formation
rate.
3. Differentiate multistage and feedback systems.
4. Discuss super critical fluid extraction.
5. Explain C Bourdon tube pressure gauge with a neat diagram.
6. Adsorption chromatography.
PART – B (5 x 10 = 50)
Answer ALL the Questions
7. Explain the chronical development of fermentation industry.
(or)
8. Write short notes on
(a) Biotransformation (3)
(b) Clarification (3)
(c) Pure culture (2)
(d) Absorbance (2)
9. What is continuous culture? How will you determine the steady state
biomass concentration? Also, comment on the yield factor.
(or)
10. Tabulate the various groups of bioreactors currently used for
industrial production based on their performance.
11. Explain computer controlled fermenter with a neat diagram.
(or)
12. Discuss the methods of measuring process variables.
13. A small test filtration of auromycin crystals in an acetone suspension
uses a filter medium of negligible resistance. It gives the following
data:
t(sec) V(liters)
10 0.500
20 0.707
30 0.866
In finding these data, we have used a filter with 89 cm 2 area and a
pressure drop of 2.6 m of water. We now plan to filter a much larger
crop or crystals containing 7300 liters of solvent by using a filter of
1.3m2. However, this larger crop has a concentration of 0.28 g/100
cm2 solvent, less than that in our test filtration, which is 0.34
g/100cm2 solvent. How long will it take to filter this new crop at the
same pressure drop?
(or)
14. A batch steroid extraction. Water containing 6.8 mg/liter of a steroid
is extracted with initially pure methylene dichloride. The equilibrium
constant for the steroid is 170 and the ratio of water to solvent is 82.
What is the concentration in the organic after the extraction? What
fraction of the steroid has been removed?
15. Explain ultra filtration process with necessary diagram and equations.
(or)
16. Gel Permeation chromatography of Urease. We plan a large scale
purification of urease using packed calcium of polyacrylamide beads.
We obtain the following data:

Volume eluted Concentration

(liters) (Arbitary Units)
174 0.0063
190 0.0152
The bed volume is 20 liters. Find V0,  and the yield at 190 and 200
liters.