B.

Tech 3rd year 6th semester

Biochemical Engineering
End SemesterExamination (2011-12)
BIOPROCESS ENGINEERING-II (TBE-601)
Max Time – 3 hr Max Marks – 100
Note: Attempt all questions.

1. Attempt any four questions:- (4x5=20)

(a) What is scale-up? What is the effect of microbial kinetics and transport phenomena on scale-up
process?
(b) What is aeration based parameter for scale-up of fermentation processes?
(c) Explain the mechanism of mixing. Assuming mixing is a first order operation, develop an expression
to calculate mixing time.
(d) Describe the applications of fed batch cultivation.
(e) What are the advantages of fed batch operation? Describe different types of fed batch operation.
2. Attempt any two questions:- (4x5=20)
(a) Develop an expression for total biomass concentration in fed batch operation.
(b) The Zymomonas mobilis cells are used for chemostate culture in 60m 3 fermenter. The feed contains
12 gl-1 glucose. Ks for the organism is 0.2 gl-1. Yx/s= 0.06, Yp/x= 7.7, µmax= 0.3 h-1, qp= 3.4 h-1
-1
I. What flow rate is required for a steady state substrate concentration of 1.5gl ?
(c) E.coli is cultivated in continuous culture under aerobic condition with glucose limitation. When the
system is operated at D=0.2 h-1, determine the effluent glucose and biomass concentration by using
monod equation for the following parameters: S0=5g/l, µm=0.25h-1, Ks= 100mg/l.
(d) Draw the profile of substrate concentration, biomass concentration and productivity of biomass with
dilution rate and determine the optimum operating conditions.
(e) Compare the biomass productivity of batch and CSTR. Describe the effect of mass doubling time on
the selection of batch or continuous operation.
3. Attempt any two questions:- (4x5=20)
(a) What do you understand by in-line, on-line and off-line measurement system?
(b) Describe the working of polarographic dissolved oxygen electrode and enumerate its advantages.
(c) Draw and explain the working of system used for foam control. What are various antifoaming
agents?
(d) Describe the sterilization of air filter and related pipelines with the help of a suitable diagram.
(e) What are different types of viscometer?
4. Attempt any two questions:- (2x10=20)
(a) Describe the advantages of fluidized bed reactor over packed bed reactor. Write few applications of
fluidized bed reactor.
(b) Define the Damkohler no. and effectiveness factor. Describe the relation between Damkohler no and
effectiveness factor for enzymatic reactions.
(c) What is packed bed reactor? What are various modes of substrate feeding in a packed bed reactor?
5. Attempt any two questions:- (2x10=20)
(a) Differentiate between internal loop and external loop airlift bioreactor. Discuss the advantages of
external loop over internal loop bioreactor.
(b) What are different types of nozzles used in non- mechanically agitated bioreactor?
(c) What are the advantages of computer controlled fermentation process? Draw a flow chart for
calculation of biochemical parameters.