Micr22- GENERAL MICROBIOLOGY

Final Examination
LABORATORY

INSTRUCTION: You are given two days to answer this Laboratory Final Examination after
which, you may send it to our email: elvira.oclarit@vsu.edu.ph & mavelyn.caca@vsu.edu.ph.

Name: Kimberly A. Muñez Date: February 12, 2021_________

Offering No. __S038_____________ Score: _______________________

I. MODIFIED MATCHING TYPE: Match the items in Column X with those in Y.

Write
A - if the item is related to A
B - if the item is related to B
C - if the item is related to both A and B
D - if the item is not related to both A and B

Write only the letter in the answer sheet (15 pts.)

X Y
___C___ 1. Ocular Micrometer A. ODV
B. OD

___C___ 2. Compound Microscope A. Fine adjustment

B. Stage

___A___ 3. Rheostat A. Light

B. Dark

___C___ 4. Depression Slide A. Carmine dye particles

B. Bacteria

___C___ 5. Multicellular A. Volvox

B. Rhizopus

___C___ 6. Nutrient Broth A. Agar

B. Peptone

___A___ 7. Pressure Cooker A. 15 psi for 15 mins

B. 160 °C for 2 hours

___A___ 8. Culture medium A. PDA

B. TYGA

___B___ 9. Bromthymol blue (pH 7) A. Yellow

B. Green

___A___ 10. Deep colonies A. Pour plates

B. Streak plates
 ___B___ 11. Agar Stroke A. Convex
B. Beaded

___D___ 12. Cell morphology A. Bacillus

B. Coccus

___B___ 13. EMBA A. Presumptive test

B. Completed test

___C___ 14. Durham Fermentation Set-up A. Qualitative test

B. Presumptive test

___A___ 15. Presumptive test A. Qualitative test

B. Quantitative test

II. MODIFIED SELECTION TYPE: Underline the letter of the corresponding item that
does not belong to the group (15 pts.)

1. A. Stage micrometer B. Coarse adjustment C. Iris diaphragm D. Stage

2. A. LPO B. OIO C. HPO D. OOI

3. A. Condenser knob B. Coarse adjustment C. Coarse knob D. Fine adjustment

knob
4. A. Simple staining B. Endospore Staining C. Gram Staining D. Negative Staining

5. A. Gram iodine B. 95% ETOH C. safranin D. Methylene blue

6. A. Depression slide B. alcohol C. Cover slip D. Water (seal)

7. A. Bromthymol blue B. phenolphthalein C. bromcresol green D. basic fuchsin

8. A. Lactose broth B. EMBA C. Nutrient Broth D. Nutrient Agar

9. A. peptone B. distilled water C. beef extract D. yeast extract

10. A. convex B. lobate C. pulvinate D. umbonate

11. A Filiform B. Filamentous C. Beaded D. Rhizoid

12. A. Coccus B. Coccobacillus C. triplococci D. diplococci

13. A. Escherichia B. Klebsiella C. Paramecium D. Enterobacter

14. A. Coliform test B. Presumptive test C. Confirmed test D. Completed test

15. A. Filiform B. Filamentous C. Beaded D. Arborescent

III. ANALOGY. Write on your answer sheet the word that fit the given word analogous
to the first given pair (15 pts.)
Example: HPO: 40x; SO: 4x
1. Stage micrometer: Stage 2. Fine Adjustment Knob: HPO
Ocular micrometer: Eyepiece_____ Coarse Adjustment Knob: LPO_

3. Stage finger: Holds slide on the stage 4. Gram positive: Blue

Stage: The slide is placed________ Gram negative: Red_________

5. Ammonium oxalate crystal violet: Gram 6. Hay Infusion: Negative Staining

staining Teeth scrapings: _Simple staining_
Malachite Green: Endospore staining_

7. Glassware’s: dry heat 8. pH 9: phenolphthalein

Culture media: ___Moist heat_______ pH 7: _ Bromthymol blue ____

9. Nutrient broth: liquid 10. Aspergillus: multicellular

NA: ____Solid___________________ Nostoc: ___Unicellular________

11. Escherichia coli: gram negative 12. Circular: margin

Bacillus subtilis: __Gram positive____ Opaque: __form________

13. Presumptive Test: Lactose Broth 14. Fecal coliform group: Escherichia
Coliform Test: ___Qualitative______ Hepatitis: Gastrointestinal disease_

15. Aerobacter: whitish colony

Escherichia: __anaerobic bacterium___

IV. TRUE or FALSE: Write TRUE if the statement is correct and FALSE if otherwise.
Write your answer on the answer sheet (25 pts.)

__TRUE___ 1. The fine adjustment knob is used in focusing objects under the oil
immersion objective.

___TRUE__ 2. The ocular micrometer is used to calibrate the stage micrometer.

__FALSE__ 3. Average length of Paramecium varies significantly as magnification is

increased.

__FALSE___ 4. The oil immersion objective must be cleaned immediately with a tissue
paper after using the microscope.

__TRUE__ 5. The value of each division of stage micrometer stays constant in each of
the different objectives.

__FALSE__ 6. The temporary wet mount involves placing live microorganisms in a

liquid medium between a glass slide.
 __FALSE__ 7. The protozoans exhibited Brownian movement.

__FALSE___ 8. Rhizopus sp. reproduce sexually through the formation of zygospores.

__FALSE___ 9. Nostoc is a unicellular fungus.

__FALSE___ 10. In the preparation of bacterial smears, air drying follows after heat
fixation.

___FALSE__ 11. Bacterial cells appear blue after negative staining.

___TRUE___ 12. Nutrient broth is a liquid culture medium made up beef extract,
peptone and distilled water.

___TRUE___ 13. Sterilization is the process of making any material entirely free from
living organisms.

___FALSE__ 14. Bromthymol blue was used to adjust pH to pH 9.0.

___FALSE__ 15. Bacteria are inhibited from growing in an acidic unsterilized medium.

___TRUE___ 16. Deep colonies can be observed in streak plate technique.

___FALSE__ 17. Raised growth of bacterial colony describes its density of growth.

___TRUE___ 18. The thick walled, resistant survival structures produced by certain
species of bacteria are the endospores.

___TRUE___ 19. The growth of bacteria on an agar slant is referred to as agar stroke.

___TRUE___ 20. A culture is pure when it contains only a single species and no other
organisms.

___FALSE__ 21. Coliform bacteria are gram negative spore formers.

___FALSE__ 22. Qualitative examination of water determines the efficiency of water

treatment.

___TRUE___ 23. Coliform bacteria serve as indicator of fecal contamination in drinking

water.

___TRUE____ 24. Positive confirmed test indicates that the water is presumably unsafe
for drinking.

___TRUE____ 25. Yellowing of broth and presence of trapped gas are indicators of
positive confirmed test.

V. ILLUSTRATION. Draw the following in your answer sheet. (10 pts).

Euglena Bacillus subtilis

Amoeba Nostoc

Yeast Spirogyra

Escherichia coli Diatoms

Volvox Rhizopus sp.

VI. PROBLEM SOLVING. Read each problem below and solve what is being called for.
Show the calculations (20 pts.)

1. A thesis student measures the length of Paramecium cells using the ocular
micrometer. The following data were obtained.
No. of Divisions on Ocular Micrometer occupied by Paramecium length
Paramecium LPO HPO
Cell No.
1 10 23
2 12 26
3 16 28
ODV at LPO = 10 μm/div
ODV at HPO = 2.5 μm/div

Questions: Compute for the average length of Paramecium under HPO. (5 pts)
Average number of division
HPO= 23+26+28=77
77÷3=25.67

Actual Lenght at HPO

25.67div × 2.5um/div = 64.18 um

2. A laboratory worker needs to prepare 2.5L Nutrient Agar. How much of each of
the ingredients does he need to weigh or measure? (5 pts)

In 5 liters
3 grams of beef extract x 5 = 15 grams of beef extract
5 grams of peptone x 5 = 25 grams of peptone
15grams of agar x 5 = 75 grams of agar

Half of 5 liters is 2.5 liters Therefore in 2.5 liters

3 grams of beef extract x 2.5 = 7.5 grams of beef extract
5 grams of peptone x 2.5 = 12.5 grams of peptone
15grams of agar x 2.5 = 37.5 grams of agar

Answer: In 2.5 liters of nutrient agar, add 7.5 grams of beef extract, 12.5
grams of peptone and 37.5 grams of agar

3. A laboratory worker was requested to determine the concentration of bacteria in a

water sample in colony-forming units per ml. From the sample, he got 1 mL and
transferred it to a 9 mL blank to make the first dilution (1:10). From the first
dilution, he got 1 mL and transferred it to a 9-ml blank for his second dilution
(1:100), and from his second dilution, he got 1 mL and transferred it to another 9 -
mL blank for his third dilution (1:1000). He then got 1ml from each dilution tube
 and transferred into a melted Na and poured into corresponding plates. After
incubation, he had the following colony counts in the plates.
1st dilution: Plate 1 = 850 2nd dilution: Plate 1 = 160 3rd dilution: Plate 1 = 3
Plate 2 = 725 Plate 2 = 150 Plate 2 = 4

Questions:
a. Which dilution will you consider in the computation and why?
Ans. I will choose the 2nd dilution because we need to select the plates with an
average count that is between 30 to 300 colonies.

b. What is the dilution factor of the dilution you have chosen in a?

Ans. The dilution factor of 2nd dilution is 100.

c. Compute for the bacterial concentration of the water sample in cfu/ml.

Ans.
Dilution Dilution of Water Dilution Factor Average
Sample (where Number of CFU/ml
colony count is Colonies
within 30-300)
nd
2 dilution 160 + 150/1: 100 100 155 15,500 cfu/ml
Average Number of Colonies
160+150= 310
310÷2=155
Bacterial concentration of water
155 x 100 = 15,500 cfu/ml

BONUS: (10 pts)

1. "What did you learn from the coronavirus pandemic?"
I learned that we should be able to be resilient at all times in any kinds of
situations and challenges that comes to us. This is because life during the current
pandemic that we are facing as of this moment is very sad. Also, we should always take
care of ourselves especially our health and immune system. Being cautious of our
surroundings is also a must in order to keep ourselves in good health and avoid
contracting the coronavirus. Lastly, always keep in our minds that prevention is better
than cure.

GOOD LUCK and GOD BLESS YOU ALL!!