CHALLENGE QUESTIONS (Write your answer/s here for practice)

QUESTION ANSWER
1. For routine staining. Hematoxylin and eosin (Ha and E)
2. Mast cell tumor granule detection. Toluidine blue
3. For Mycobacterium staining. Ziel-Neelsen Staining
4. For fungi staining. Gomori’s silver
5. For gram + and – bacteria staining. Brown-brennan (B&B)
6. For spirochetes staining. Levaditi’s method
7. For amyloid staining. 1. Congo red
2. Crystal violet
8. For carbohydrate staining. Periodic acid-Schiff reaction (PAS)
9. For fat or lipid staining. Oil red (frozen sections)
10. For glycogen staining. Best’s carmine
11. For calcium salt staining. Von Kossa’s
12. For myelin staining. Luxol fast blue (LFB)
13. For collagen staining. 1. Masson trichrome
2. Van Gieson’s stain
14. For elastin fiber staining. Van Gieson’s stain
15. For connective tissue staining. Masson trichrome
16. For cross striation of muscle. Phosphotungstic acid-hematoxylin
(PTAH)
17. Examples of Romanowsky stains. 1. Wright’s
2. Wright-Giemsa
3. Giemsa
18. Examples of Supravital stains. 1. Brilliant Cresyl Blue
2. Crystal Violet
19. Removal and examination of tissue Biopsy
from a live animal.
20. Commonly used fixative. Micro-anatomical fixatives
21. Decalcifying agent. Gooding and Stewart’s fluid
22. Commonly used dehydrating agent.
23. Alternative dehydrating agents. Dioxan, THF and Cellosolve
24. Commonly used clearing agent. Xylene
25. A white, more or less translucent, Paraffin
odorless, tasteless and waxy solid.
26. Thickness of tissue for proper fixing. 1 cm
27. Required cutting size of the block in a
microtome.
28. Time required for hematoxylin and
eosin staining.
29. Examples of acidic structures in the cell.
30. Examples of basic structures in the cell.
20 um or average of 5 microns
15 minutes
ribosomes, glycosaminoglycans
the cytoplasm and intercellular
material