Name: Hyacinth DJ.

Garcia Date: July 3, 2021

Microscope Worksheet
I. How Can a Microscope Help Us Study Living Things?
Microscope: an instrument that makes things look bigger
Biology: the study of living things.
Some living things are so small we can’t see them with the naked eye alone. Many of these are one
celled organisms called PROTOZOA [pro tuh ZO uh] and BACTERIA.
We can see such tiny things with the help of a high-power microscope. A microscope magnifies, or
makes things look bigger.
Have you ever used a magnifying glass? It is a simple microscope.
A magnifying glass, or hand lens, is easy to use. It doesn’t weigh much, and it’s easy to carry. But it
doesn’t magnify objects very much. We still cannot see one-celled plants and animals with a hand lens.
A compound microscope is much more powerful than a simple microscope. Most school microscopes
are compound microscope. They can make objects look 100 to 400 times as big as they really are. Some
compound microscopes magnify more than a thousand times. When we say “microscope,” we usually mean a
compound microscope.
The microscope has many uses, especially in biology – the study of living things.
Doctors often use microscopes. Is there one in your doctor’s office?

Parts of the Microscope

Eyepiece: Lens closes to the eye.
High Power Objective: Longer of the two lenses close to the slide.
Lower Power Objective: Shorter of the two lenses close to the slide.
Tube: Gives the distance needed between the eyepiece and objective.
Coarse Adjustment Knob: Moves the tube up and down.
Fine Adjustment Knob: Also moves the tube up and down but only slightly.
Base: Holds up the entire microscope.
Arm: Holds the tube and lenses. Connects to the base.
Nosepiece: Holds objective lenses.
Light: Shines light into the tube.
Diaphragm: Circular disk that lets more or less light pass through.
Stage: Platform for slide to rest. An opening in its center lets light pass through.
Clips(2): Hold the slide in place on the stage.

II. How Does a Microscope Magnify?

There are three lenses in the microscope. The Eyepiece Objective Lens Magnification
top lens is called the eyepiece. It is the lens closest to 10x 10x 100x
the eye. The other two lenses are called objectives. 10x 40x 400x
These are the lenses closest to the object. The object is on 10x 44x 440x
a microscope slide. Different lenses magnify in different 5x 10x 50x
powers. The power of magnification is marked by a 5x 40x 200x
number with an x next to it. A lens that magnifies ten 20x 10x 200x
times is marked 10x.
If the eyepiece is marked 10x and the objective 10x, then the total magnification is 100x. To find the total
magnification of a microscope, just multiply the two magnifications. Complete the table below by finding the
total magnification for each pair of lenses.
III. Handling Microscope. A microscope is a delicate instrument. Treat it carefully.
1. A student in the figure below is holding the microscope properly. Can you describe the right way to hold a
microscope?

Remove the microscope from the cabinet

by firmly grasping the arm with one hand and supporting the

equipment with the other. Remove the dust cover only after the

microscope has been set up and positioned on the table where

it will be used all of the time.

2. The boy is wondering what to use to clean the microscope lenses. The lens tissue has been treated to prevent
scratching. What should one use to clean? Kimwipes Why? Because kimwipes, they clean
equipment and instruments for laboratories, camera lenses, optical fibers and other sensitive electronic devices
3. The girl is focusing a slide and she is moving the stage up toward the slide.
a) What has happened to the slide?
The slide isn’t positioned right
b) Is this the proper way to focus?
No, the slide must move up and down in order to see the object.
c) Should you focus by moving stage up or down?
Yes because this is the proper way to focus
d) What happens to a microscope if place at the edge?
There is a possibility that it will fall over
e) What should you do to prevent this from happening?
Make sure it's far enough away from the table's sides to allow you to see through it.

VI. Matching. Match the two lists. Write the correct letter on the line next to each number.
1. d simple microscope a) support entire microscope.
2. a base b) has one lens only.
3. b compound microscope c) study of living things.
4. e eyepiece d) has more than one lens.
5. c biology e) lens closest to the eye.

VII. True or false. Write T on the line next to the number if the sentence is true. Write F if the sentence is
false.
1. f 9. f
2. t
3. f
4. t
5. f
6. t
7. f
8. t
A tricky!) A microscope can have one lens.
Compound microscopes are used on nature walks.
m A compound microscope magnifies more than a simple microscope does.
i Light enters the eyepiece first.
c A microscope stage must have an opening.
r When you carry a microscope, you should hold it by the tube.
o Focusing upward can crack a slide.
s You should use only lens tissue to clean a microscope lens.
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PARTS

1. Identify each of the following by letter.

K On/Off Switch C Eyepiece / Ocular Lens

J Arm F Nosepiece

G Objective Lenses D Stage

H Diaphragm E Stage Adjustment

M Base L Coarse Adjustment

A Lens Paper N Fine Adjustment

B Immersion Oil P Slide box

O Kimwipes

MICROPARA-SUMMER 2021
2. Explain what each of the following does:

kimwipes These wipes were created for cleaning surfaces where a small amount of lint or dust could
interfere with the equipment's or electronic item's normal operation. Used to clean laboratory
instruments and equipment.

immersion oil More light is focused through the objective when using immersion oil, resulting in a clearer
image. Exclusively with a 100X or higher objective lens.

diaphragm The diaphragm is a five-hole disc that sits beneath the stage. It enables us to control the amount
of light that enters the stage opening.

stage adjustment knob The mechanical stage lets you to move the slide forward, backward, and to the left or right.

Plant Cells

PASTE A PLANT CELL AS VIEWED IN MICROSCOPE. NOTE: WRITE A BRIEF CAPTION.

This is a plant cell viewed in

microscope at 40x. As you can see, the
green dots are visible. These green dots
are called chloroplasts.

Human Blood
PASTE A PICTURE OF A HUMAN BLOOD AS VIEWED IN MICROSCOPE. NOTE: WRITE A BRIEF CAPTION.

MICROPARA-SUMMER 2021
 This is a human blood cells viewed in a
microscope at 100x. At 100x, you'll notice a
larger cell with a black object in it. This cell is
different from the others since it is a white
blood cell that protects your body from
microbes.

BACTERIAL CELL

PASTE A PICTURE OF A BACTERIA CELL AS VIEWED IN MICROSCOPE. NOTE: WRITE A BRIEF CAPTION.

This is a bacteria cell (Acid fast mix) viewed in

a microscope at 100x. The bacteria cells all have
a tan backdrop with a lot of black specks. The
dots are quite little; in fact, they are smaller than
blood cells in general.

MICROPARA-SUMMER 2021