Candidate name

Centre number Candidate number

BIOLOGY 0610/6
Practice exam-style Paper 6 1 hour

Candidates answer on the Question Paper.

No Additional Materials are required.

READ THESE INSTRUCTIONS FIRST

Write your Centre number, candidate number and name on all the work you hand in.
Write in dark blue or black pen.
You may use a pencil for any diagrams or graphs.
Do not use staples, paper clips, highlighters, glue or correction fluid.
DO NOT WRITE IN ANY OF THE BARCODES.

Answer all questions.

Electronic calculators may be used.

You may lose marks if you do not show your working or if you do not use appropriate units.

At the end of the examination, fasten all your work securely together.
The number of marks is given in brackets [ ] at the end of each question or part question.

0610/3 Practice exam-style Paper [Turn over]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 1

1 The enzyme amylase digests starch to maltose. Maltose is a reducing sugar.
A student set up an experiment to investigate the effect of pH on the rate of activity of
amylase.

• She used a measuring cylinder to add 10 cm3 of starch solution to each of 5 test tubes.

• She added buffer solution at five different pHs to the 5 tubes.

• She then added 2 cm3 of amylase solution to each test tube.

• Every minute, she tested the contents of each tube for starch.

She recorded the time it took for the starch to completely disappear from each tube.
These are the results that she wrote down.

tube A, pH 2, starch did not disappear

tube B, pH 4, took 14 minutes for the starch to disappear
tube C, ph 6, took 6 minutes
tube D, pH 8, took 3 minutes
tube E, pH 10, took 10 minutes

a i State the variable that the student changed in her experiment.

[1]

ii State three variables that she kept the same, or should have kept the same.

3 [3]

iii The student did not have a control tube in her experiment.

Suggest how she could have set up a control.

[1]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 2

 iv The student used an electronic pH meter to measure the pH in each tube. This
meter has a probe that is placed into the liquid. The pH is shown on a digital
display. Fig. 1.2 shows a pH meter.

3.20
Temp.°C 10 20 30 pH
0 40
Ext. 50 mV
250
Meter
60
200
70
100 90 80
pH Buffer 150
Off mV

100

50

pH Meter Model 60

Fig. 1.2

Suggest why using an electronic pH meter is better than using indicator paper to
measure the pH.

[2]

b Describe how the student could test a sample of the contents of a tube for starch.

[2]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 3

 c i In the space below, construct a results chart and complete it, to show the
student’s results. [4]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 4

 ii On the grid, draw a line graph to display the student’s results.
Join the points with ruled lines. [5]

d State two significant sources of error that reduce the reliability of the student’s results.
(Do not include mistakes that the student may have made.)

[2]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 5

 e At the end of her experiment, the student tested the contents of each tube for
reducing sugar. She did this by adding Benedict’s solution, and standing the tube
in a water bath at 80 °C.

i Explain two reasons why using a water bath is better than heating the tube over a
Bunsen burner or other flame.

[2]

ii The contents of one tube stayed blue when tested for reducing sugar.
Suggest which tube this was, and explain your answer.

Tube stayed blue because

[2]

f Describe how the student could carry out an experiment to investigate the effect of
temperature on the rate of activity of amylase.

[4]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 6

2 Fig. 2.1 is a photograph of a centipede.

Fig. 2.1

a i Measure the length of the antenna labelled A on Fig. 2.1.

length = mm [1]

ii The actual length of the antenna is 50 mm.

Calculate the magnification of the photograph.

Show your working.

magnification = [3]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 7

 iii In the space below, make a large drawing of the part of the centipede in
Fig. 2.1 that is enclosed within the box. [5]

b i Use the evidence shown in the photograph to determine the group of arthropods to
which the centipede belongs.

[1]

ii Give two reasons for your decision.

2 [2]

© Cambridge University Press 2014 IGCSE Biology Practice exam-style Paper 6 8