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Parasitism
- Symbiotic relationship between two very
dissimilar organisms (nematode and
human being)
- One organism thrives while the other is
hurt in the process.
Phasmid Nematodes
● Hookworms
● Ascaris
● Parastrongylus
● Strongyloides
● Enterobius
Aphasmid Nematodes
● Trichuris
● Trichinella
● Capillaria
Nematodes
- AKA “Roundworms”
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Extraintestinal Nematodes - Usually found in the intestines of
(Lymphatic/Circulatory System) the definitive host
● Wuchereria ➔ Notable structures:
● Brugia 1) Scolex
● Panstrongylus - Anterior structures
- Used by the organism to
Modes of Infection attach to the definitive host
1) Ingestion of embryonated eggs (ex: Humans)
○ MOI: Ascaris, Trichuris, Enterobius - Can have hooks or suckers
○ Can penetrate skin (MOI: in order to help them on
Hookworms, Strongyloides) attachment
2) Ingestion of larva
○ MOI: Capillaria, Trichinella, 2) Neck
Parastrongylus - Region of growth because
3) Bites of insect vectors the segmentation or the
○ Ex: Bites of mosquitoes strobilation originates from
○ MOI: Wuchereria and Brugia it
4) Autoinfection 3) Strobila
○ You are the source of the infection 4) Proglottids
○ MOI: Capillaria, Strongyloides - Segments
(Commonly transmitted through - Proglottids closest to the
Autoinfection), Enterobius neck are the most
immature
- As we go further down the
organism it is followed in
the increase of mature
segments
➢ Gravid Segments
○ Most mature
segments as it
contains eggs,
ovum, and larva
Cestodes
- AKA “Tapeworms”
- Dorsoventrally flattened
- Segmented, ribbon-like appearance
- Incomplete digestive tract
- No circulatory system
- Monoecious
➔ Larva stages:
- Usually found in the tissues of the
intermediate host
➔ Adult tapeworms:
- Are hermaphroditic
2. Ventral Sucker called the
Clinically Significant Tapeworms: Acetabulum
● Cyclophyllidea 3. Genital Sucker called the Gonotyl
● Pseudophyllidea (Only for Heterophyids)
- Monoecious (Both male and female
Mode of Infection of Cestodes reproductive structure)
➔ Generally: Ingestion of infected - Can have Operculated eggs
intermediate hosts - Grouped together according to their
habitat
Pseudophyllidean Tapeworms
➢ Bothria
○ Scolex is Spatulate in shape
○ Have sucking grooves
➢ Has aUterine Pore
○ Allows the release of the egg from Two Intermediate Hosts
the gravid uterus First:
➢ 2 intermediate Hosts - Snail
➢ Diagnosis:
○ Operculated eggs Second:
- Fish
Cyclophyllidean Tapeworms - Crustacean
➢ Globular Scolex - Freshwater plant
○ Circular with four muscular - Snail
suckers
➢ Undergo the process of Apolysis Infective Stage
○ Gravid segments detach from the - Generally: Metacercaria
main body of the worm and after - For Schistosomes: Cercaria
which the eggs are released
➢ Does not have uterine pores
○ They therefore do not release their Intestinal Nematodes
eggs
➢ 1 Intermediate Hosts Ascaris lumbricoides
➢ Diagnosis: ➢ Common Name:
○ Non-operculated eggs ○ Giant Intestinal Roundworm
○ Segments ○ Common Roundworm
➢ Generally Distributed: Mostly
Trematodes Cosmopolitan
- AKA “Flukes” ○ Most common Helminths found in
- Equipped with suckers: the Philippines, Indonesia and
1. Oral Sucker other SEA countries
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Often found in rural areas
○ - Brownish because they are bile-stained
■ Rates often exceed greater - Contain a blastomere which develops
than 50% in Rural areas into an embryo
➔ Disease: Ascariasis - Have a semilunar space at each pole of
the blastomere (or the embryo).
- Can have a three-layered shell
○ Layers:
■ Vitelline membrane
■ Thick chorionic shell
■ Albuminous layer
- Can float in a saturated solution of
common salt
Prevention
Life Cycle of Ascaris Lumbricoides
- Do not eat unclean food and water
1. Adult worms (male and female) within the
- Make sure to wash hands
small intestine of the human.
2. Worms copulate fertilized and unfertilized
Laboratory Diagnosis
eggs which can be deposited to the soil.
● Finding larvae in sputum or gastric
3. The unfertilized egg will not undergo
washings
further development.
● Stool exam
4. While the fertilized egg can undergo
● Identification of adult worms in stool,
further development from an embryo and
vomitus, nose, or ears
into a third stage larva.
5. When human beings ingest the
embryonated eggs, they become infected
with ascaris lumbricoides (can be found in
improperly prepared food, oral-anal
contact, etc.)
6. Once ingested, they travel through the
mouth → esophagus → stomach → small
intestine → large intestine
7. They are able to undergo lung migration The violet body cavities of parasite and the
(Hatched larva can enter the circulation tissues of the patient can be seen
and migrate to the lungs)
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○ More common in warm, moist
regions of the world
Barium Swallow ➔ Host: Man (Only known host)
- Can be used to diagnose patient with ➔ Habitat: Cecum and other parts of the
Ascaris lumbricoides large intestine
- Radiographic study with Barium sulfate
- Adult worms either take up BaSO4 or not
- Worms are seen on the x-rays as linear
shadows
➢ Tramway sign
○ A group of worms lying parallel //
to each other in the host intestinal
lumen (“trolley car lines”)
Treatment
● Individual infections are cured by a single
dose of any of the broad-spectrum
anti-helminthics such as
○ Albendazole, Mebendazole, and
Pyrantel Pamoate Adult worms are grossly flesh-colored. They are
● Ivermectin has been shown to be as covered by a transversely striated cuticle. The
effective as Albendazole posterior end is thicker than the anterior end and
resembles the handle of a whip and the anterior ⅗
Trichuris trichiura looks like the whip portion. The esophagus of this
- Common name: “Whipworm” parasite is very long and occupies approximately
○ Because adult worms look like ⅔ of the length and it is surrounded by a large
“whips” unicellular gland called stichocytes.
- Other names:
○ Trichocephalus trichiurus Male worms: 30-45mm in length and their
○ Trichocephalus dispar caudal extremity is coiled 360º or more. They
➔ Diseases: have a single lanceolate spicule which protrudes
○ Trichuriasis through a retractile sheet.
○ Trichocephaliasis
○ Whipworm infection Female worms: 35-50mm in length and are
➔ Geographic distribution: Cosmopolitan longer and bigger than that of the males. They
have a blunty rounded posterior end that has a
single genital system. One gravid female can lay
1000 eggs per day. Trichuris trichiura eggs are
much less resistant to desiccation and heat
compared to that of Ascaris. They do not develop
to the infective stage on hard clay and ashes. Laboratory Diagnosis:
They also do not survive in the direct sun’s rays ● Demonstration of characteristic eggs in
or even in cold temperatures. patient’s feces
○ Sometimes, it is necessary to
quantify the amount of eggs
present in the patient’s sample so
that we can relate this to the type
of infection the patient has.
■ Light, Heavy, or Massive
infection can be
determined based on the
number of eggs that is in
the sample
● Light Infection:
Less than 10 eggs
per smear
● Heavy Infection:
Over 50 eggs per
smear
● Massive Infection:
Life Cycle of Trichuris trichiura
Eggs are too
1. Unembryonated eggs are passed through
numerous to count
within the stool and these are deposited
per smear
within the soil.
● Of great value in the diagnosis of clinically
2. In the soil, they develop into a 2-cell stage
significant infection is examination of the
and eventually become embryonated
rectal mucosa by means of proctoscopy
(infective stage)
or examination during rectal prolapse.
3. Humans will ingest the embryonated
○ Proctoscopy: Examine rectal
eggs, these would pass through the
mucosa and is especially
alimentary tract and eventually reach the
important if the patient has rectal
small intestine
prolapse
4. In the small intestine, the unembryonated
■ The rectal prolapse and the
eggs would eventually hatch
rectal mucosa passes
5. These would migrate down to the large
through the rectal canal
intestine where they would populate and
and passes through the
eventually again, lay eggs
anus and is found outside
6. The eggs will be passed through the feces
of the body
Mode of infection:
- Ingestion of embryonated eggs
Capillaria philippinensis
➔ Disease caused: Intestinal Capillariasis
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- Called Capillaria philippinensis because
the first case was in the Philippines
➢ Historical Background:
○ 1st case (1963): Male from Ilocos
Norte died 3 days after admission
to PGH with a diagnosis of
malabsorption syndrome
○ 1967: in Pudoc, Ilocos Sur, an
adult male displayed severe
symptoms
○ 1967-1970: 1,400 cases and 95
deaths
○ 1988: a total of 1,877 cases with
115 deaths
➔ Generally Distributed: Northern Luzon,
Leyte, and Thailand
Diagnosis
● Stool Exam
● Demonstration of adult worm - Eggs are elongate, ovoidal,
● Scrapings from the intestinal wall (Or asymmetrical, flattened on one
Intestinal content) side
● Intradermal skin test - Letter D
- Shell is relatively thick and
Treatment and Prevention colorless
● Mendebazole is the drug of choice - Embryonated when laid; infective
● Albendazole is alternative within 4-6 hours after being
● In severe cases, electrolyte replacement is deposited
given to the patient and the patient is put - Habitat: Cecum, appendix,
on a high-protein diet ascending colon
○ Due to the presence of Adult:
malabsorption and patients have
not been getting the adequate
amount of protein for weeks or
months
● Prevention of the spread of this infection:
sanitation and health education programs
to prevent the indiscriminate disposal of
human waster;
○ Discouraging the consumption of
raw/undercooked fish is also
important to control the spread of - White, small, sometimes brownish in color
infection - Oral ends has 3 lips; lack true buccal
capsule
- Has cephalic alley- bladder like expansion
Enterobius Vermicularis of cuticle
ENTEROBIUS VERMICULARIS - Male: posterior ends is posteriorly curved
- Common name: pinworm, - ”seatworm”
seatworm - 2-5 mm
- Other name: Oxyuris vermicularis - Posterior end strongly curved
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- Single copulatory spicule; lack - Patient is his/her own source of
grubarni infection
- Female:
- 9-13 mm Airborne
- Tail sharply pointed - Eggs are dislodged from linens
- Vagina- long extended posterior in and get into the mouth, or are
the vulva inhaled and swallowed
- ”Pinworms” - May remain in the nose until they
- Gravid females= 11105 ova/day hatch
Pathogenesis
- Pruritus ani= severe itching in the
perianal and perineal skin;
dermatitis around the anus is
present migration of egg-laying
females
- Appendicitis (2%)
- Mild eosinophilia
- More frequent infection in young
- Male dies, and female reaches the persons; 3x more in women > men
cecum - Vaginitis in girls: mucoid vaginal
- 12-14cm per hour discharge
- Female exits anus in the evening, - Children with heavy infection;
and lays eggs in perianal area nervousness, loss of appetite,
- Dies afterwards restlessness, irritability,
- Prefers to move at night (nocturnal nightmares, grinding of teeth, and
migration), need for oxygen bed wetting
Lab Diagnosis
Treatment
1) Drug of choice
(Mebendazole 100 mg single oral dose) or
albendazole (400 mg single oral dose)
Hookworm
HOOKWORMS
(Common hookworms of man)
- Do not reproduce within the host
- Infects primarily middle aged persons
Synonym: old world hookworm
Disease: Ancylostomiasis
Geographic distribution
- Europe
- Asia
General Characteristics
- Pathogenesis: Lesions on the intestinal
mucosa, anemia (chronic, nutritional
deficiency type), eosinophilia,
pneumonitis, erythema, dyspnea
- Habitat: small intestines
- Intermediate host: none
- Reservoir host: hogs, dogs, cats, lions,
tigers, and gorillas
- Infective form: Filariform larva
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- Dipartite
NECATOR AMERICANUS
- Synonym: New World Hookworm,
American Hookworm, American Murderer
- Disease: Necatoriasis, Uncinariasis
- Predominant human hookworm in the
Philippines
- Important agent in the production of
“laziness” (Southern USA) and “tropical
anemia” (Puerto Rico and other tropics)
The eggs of the parasites are elongated Ovoid
shape they are unembrianated and it is covered
in delicate shell
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● Meningeal lesions may be
appreciated with the use of CT
scan
➔ Treatment
● No antihelminthic treatment is
recommended at present, however
MEBENDAZOLE and
ALBENDAZOLE have been
demonstrated to effectively treat
the disease in China, Taiwan, and
Thailand
● Ocular disease may require
surgical removal of the worms
from the eyes
➔ Pathogenicity: ● Symptomatic treatment with the
● Incubation period is usually 6 to 15 use of analgesics and lumbar
days, but may vary from 12 to 47 puncture can relieve the
days headaches experienced by the
● Chief complaint: acute, severe patient
intermittent occipital or bitemporal
headache (it's because of the
migration of the parasite to the
brain causing inflammation around
the meningalining of the brain,
sometimes they could migrate to
the lungs or eyes)
● Inter-ocular hemorrhage and
retinal detachment are some
known associated complications
(if they migrate to the eye )
➔ Diagnosis:
● Diagnosis is difficult as the primary
site of infection is the brain
● Eosinophilia in the blood may
comprise 7 to 36% of the WBC
count; 90% eosinophilia in the
CSF (>10% eosinophilia in
proportion of WBC will exclude
other common causes of
meningitis)
● Larvae and young adult worms
may be seen in the CSF
● Histologic identification of the
adult female worm
● Serologic techniques
(Picture of a Wuchereria bancrofti on the left,
Brugia malayi on right)
(on the left pic the cephalic space on top its short
and the nuclei of the parasite its much more
cleaner and organized compared to the right the
tail tapers a point with no nuclei present. The one
in the right the head space is the one u see in the
7 oclock position of the pic the nuclei are
disorganized and the tail tapers to two nuclei
connected to a fine thread)
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(When the worms enter the lymphatic system or
(The parasites can lead to lymphedema
the peripheral circulation they can cause
[abnormal swelling in the lymph tissues in the
deactivation of immunologic response, they can
arms, legs, breasts and even the genitals.] The
both active the humoral and cell mediated
parasite can also cause elephantiasis [disfiguring
response, when the larva or the adult worm when
or disabling of the lymphedema of the limbs
they reach the lymphatic system they most often
breast, genitals, legs and arms with
cause obstruction and when they die they can
accompanying marked thickening of the skin] )
elicit a more severe reaction and eventually they
can cause classification which can lead to a
➔ Diagnosis
obstruction, most patients would present acute
● Good clinical history
perllarial disease (acute attacks) where in they
○ Recurrent febrile episodes
would tell you they are experiencing pain,
associated with pain,
swelling, tenderness in the affected areas would
tenderness and swelling of
include the limbs, genitals, or even the breasts.
limb, genitals or breasts
These attacks may or may not have fever, in
○ History of
males it can cause epididimo bronchitis
exposure-prolonged stay in
(inflammation of the epididymis in the scrotum)
endemic area
● Identification of microfilariae
through microscopic examination
is the most practical diagnostic
procedure
● It is important to time the blood
collection with the known
periodicity of the microfilariae (the
parasites work more at night so its
better to collect them at night)
➔ Specimens
● Blood
○ Identify microfilariae of
Wuchereria bancrofti,
Brugia malayi
● Wet smear
○ Thick smear (giemsa or
Hematoxylin and Eosin)
● Concentration or filtration
techniques - for increased
sensitivity
○ Knott’s technique
○ Filtration through a
Nucleopore membrane
(These are the adult worms of Schistosoma
japonicum they have a separate sexis for males
Trematodes
and females. The female is the one in the
● Schistosoma japonicum
“spaghetti looking thing” that is bent all over, the
● Paragonimus westermani
thicker structure is the male. The males are
shooter but more stouter and they have a
Schistosoma japonicum
gynaecophoric canal, it is the canal where the
● C.N.: Oriental Blood Fluke
females are lodge that is why they are called
● G.D: in the Phil. = Leyte, Samar,
eternal honeymooners because the females are
Mindanao, Mindoro and
attached to the male. The female's muscular
southwestern Luzon
pharynx is absent and intestinal are united to
● Symptoms of Schistosomiasis:
form a single cycom.)
○ Profuse dysentery,
Enlarged liver and spleen
○ Recurrent daily fever, Loss
of appetite and loss of
weight
○ Epigastric pain and
tenderness, Anemia
Diagnosis:
● Specific - Recovery of eggs from
feces
○ Direct examination
(it is oval to rounded shape with a short lateral ○ Concentration method =
tubercle, the arrow points the short lateral Formalin Ether Conc. Meth.
tubercle) ○ Proctoscopic aspiration
and biopsy (Valve of
houston)
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● Serologi crests (using adult and
cercarial antigens)
○ Complement fixation with
adult Ag ( CFA)
○ Complement fixation with
carcarial Ag (CFC)
○ Slide flocculation with
cercarial Ag (SFC)
○ Cercarial agglutination (CA)
○ Circumoval precipitin
(COPT)
● Intracutaneous test (skin test) -
utilize adult and larval Ag
● WBC and Differential count
Paragonimus westermani
● C.N. = Oriental Lung Fluke
● Dss.= Paragonimiasis, Pulmonary
Distomiasis, Or Endemic
Hemoptysis
● G.D. = Worldwide; Phil. - Leyte,
Samar, Mindanao (Cotabato),
Sorsogon and Camarines
● In heavy infection: ectopic foci
(mesentery, pleura =, brain and
liver)
● Symptoms:
○ Pulmonary condition =
resemble pulmonary TB;
caught and Hemoptysis
○ Abdominal type =
abdominal pains and
diarrhea
○ In the Brain = associated
with Epilepsy
● Chest X - Ray findings = not
DEfinitive but give ideas as to the
extent and progress of the
(The adult can either be egg shaped, thick fleshy disease.
or even sometimes coffee bean shaped, the oral
sucker at the tip the ventral sucker is at the Cestodes
middle of the parasite. It is hermaphroditic [it has ● Taenia solium
both male and female reproductive structure]. ● Taenia saginata
The ovum on the right has an ovodal and a flat
tuberculoma the shoulder looking structure. They Taenia solium
are thicken at the posterior end and they are ● Synonym: pork tapeworm; Armed
golden brown in color) tapeworm of Man
● Dis: taeniasis or Pork tapeworm infection between left and right side. The gravid proglottid
is common among those eating raw or the uterus is distended, it is the midline structure
insufficiently cooked “measly pork” by which there are 7 to 13 lateral branches on
● GD: cosmopolitan, prevalent in countries each sides. One segments contains at least
where raw or insufficiently cooked pork is 40,000 eggs and the terminal gravid segment
consumed would separated from the parasite in groups of 5
or 6, these can be passed out in the stool or
move them selves out of the anus, they are
inactive and flabby but they do not creep)
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(Taenia saginata scolex looks like, does not have
hooklets, it only has a white semi transparent a
grossly however it was stained in red thats why
its looks like this. It measures up to 4 to 10
meters and sometimes up to 25 meters. The
proglottids can reach to 1000 to 2000 proglottids.
The scolex is up to 1 to 2 mm, it is quadrant and
4 hemi spherical suckers at the four angles of the
head, no rostelum and no hooks.)
Laboratory diagnosis:
● Recovery of gravid proglottid (uterine
branches) in feces recovery of ova in
feces (cannot be differentiated from T.
saginata)
● Recovery of scolex after therapy
● For cysticercosis:
○ History of taeniasis
(These are the Mature segments and the Gravid
○ Biopsy of SC nodules (invaginated
segments, for the mature segments it can have
scolex with 4 suckers and and t.
both male and female reproductive structures
Circles of hooklets)
where the two lobes are the ovaries, the mature
● X-ray, CT scan of brain Skin test Precipitin
segments looks similar to the Taenia solium. The
test, C-F test
gravid segment the distal fit of the worm is
narrower and longer than the prod, the uterus has
Taenia saginata
15 to 20 lateral branches, they can have
● Beef tapeworm; “Unarmed Tapeworm of
independent movement and force their way to
Man”
the anus sphincter.
● Disease: Taeniasis saginata, beef
tapeworm
● Infection
● GD: cosmopolitan, prevalent in countries
● Where raw beef is eaten
(this is the larva of the Taenia saginata swifty
circus bovis this is usually found in kettles and
they are ovodale in shape and they are fluid filled
and one vaginated colex with four suckers and
no hooklets. The ovum looks similar to the Taenia
solium and has similar life cycles but instead of
pigs its kettles.)
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