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Culture Documents
13
NATURAL PRODUCTS
AND
CHEMICAL ANALYSIS METHODS
By Omer Bayazeid
Natural Products:
• A natural product is a chemical compound or substance produced
by a living organism. They may be extracted from tissues of
plants, marine organism or micro - organism fermentation.
1.Maceration:
3.Decoction:
- Sublimation.
- Distillation.
- Fractional liberation.
- Fractional crystallization.
- Chromatography.
Collection of medicinal plants:
c) Use of stoves.
2. Slow evaporation.
3. Refrigeration.
d) Fractional distillation:
Used for the separation of components of volatile oils.
e) Sublimation:
We use Sublimation to isolate caffeine from tea and to
• A herbarium is a collection of
preserved plant specimens. These
specimens may be whole plants or
plant parts: these will usually be in
a dried form mounted on a sheet
but, depending upon the material,
may also be kept in alcohol or other
preservative.
NAPRALERT:
1. Dragendroff’s test:
2.Mayer’s test:
4. Wagner’s test:
• Raymond’s test:
Test solution when treated with dinitrobenzene in hot
methanolic alkali, gives violet color.
• Legal’s test:
Treat the extract with pyridine and add alkaline sodium
nitroprusside solution, blood red color appears.
• Bromine water test
Test solution when treated with bromine water gives yellow
precipitate.
Test for Saponin Glycosides:
• Froth Test:
Place 1ml solution of drug in water in a semi-micro
tube and shaken well and noted for a stable froth.
• Hemolysis test:
Add 0.2ml solution of saponin (prepared in 1%
normal saline) to 0.2ml of v/v blood in normal saline
and mix well, centrifuge and note the red
supernatant compare with control tube containing
0.2ml of 10% blood in normal saline diluted with
0.2ml of normal saline.
Test for Anthraquinone Glycosides:
Borntrager's test:
Boil the test material with 1ml of dilute sulphuric acid in a test
tube for 5min (anthracene glycosides are hydrolyzed to aglycone
and sugars by boiling with acids) centrifuge or filter while hot,
filtrate, cool and shake with an equal volume of dichloromethane
(the aglycones will dissolve preferably in dichloromethane)
separate the lower dichloromethane layer and shake with half its
volume with dilute ammonia.
Stationary phase:
Stationary Phase:
Spray reagents:
Stationary System:
Spray reagents:
Specific detection of the y-lactone ring of cardenolides:
Kedde reagent Immediately on spraying, cardenolides
generate a pink or blue-violet (vis) colour. The colour fades
after a few minutes, but can be regained by repeated
spraying. Raymond reagent also give red, red-orange or
violet (vis) cardenolide-specifics colors.
(5) TLC of Coumarin:
Solvent System:
Stationary Phase:
Spray reagents:
Stationary Phase:
Spray reagents:
Toluene-chloroform-ethanol (40:40:10)
Stationary Phase:
Spray Reagents:
Chloroform-methanol-water (70:30:4)
Cloroform-metllanol( 90:lO)
Stationary Phase:
Spray reagents:
50% ethanolic sulphuric acid for Cubebae fructus
Vanillin-phosphoric acid reagent for Eleutherococci
radix.
(9) TLC of essential oil:
Solvent System:
Stationary Phase:
Spray reagents: