MAP Kinase Signaling

MAPK/Erk in Growth and Differentiation Pathway

RTKs Integrins

Ion Channels RTKs

[Ca2+]

SOS Ras SOS Tal

GRB2
Rap1
FRS2

CAS RACK1
Shc
IRS
GRB2
PLCγ Fyn

CRK
Spry
Pax FAK C3G
PI3K
EPAC
[Ca2+] Src
Raptor mTOR
PYK2 PAK Rac [cAMP] Signaling
Spred
AMPK
c-Raf PKA rpS6 TCPβ
PKC
c-Raf B-Raf B-Raf eIF4B IκBα
Heterodimer 14-3-3 LKB1
PD98059 Filamin A
MEK1 Tpl2/Cot1 U0126
LAMTOR2 AZD6244
MP1 IMP PP1/
Ion Channels, PP2At FMK
Receptors C-TAK1 KSR SL0101
1/2 BI-D1870 eEF2K GSK-3
Erk1 SOS
cPLA2
MEK1/2 Bim DAPK METTL1

Translation p90RSK BAD nNos

Late Endosome MNK1/2 Erk1/2
Control

Erk1/2 p90RSK MKP-3 TSC2

Cell Adhesion PEA-15 Cytoplasmic Anchoring Ca2+-regulated

PLD1
WAVE-2 Migration Exocytosis

lasm
Cytop
cdc25 PPARγ
us
Nucle

p27 KIP1 p90RSK BUB1

MKP-1/2 Erk1/2 MSK1/2

ER Ets Elk-1 TIF1A FoxO3 CREB ATF1 ER81 SRF c-Fos ERα Nur77 MITF C/EBPβ

c-Myc/
Stat1/3 N-Myc Pax6 c-Fos Histone HMGN1 ETV1 TIF1A ATF4 Myt1 Mad1 YB1
H3
UBF

Transcription

For pathway key and background please visit:

www.cellsignal.com/cstmapk
 Key Products for MAP Kinase Signaling
Visit www.cellsignal.com/mapklist for full listing of antibodies, related kits, and reagents.

ANTIBODIES APPLICATIONS REACTIVITY

Phospho-c-Jun
#8662 ASK1 (D11C9) Rabbit mAb WB, IP H, M, R, Mk
(Ser73) (D47G9) XP®
#3765 Phospho-ASK1 (Thr845) Antibody WB H, (M) Rabbit mAb #3270:
#9226 ATF-2 (20F1) Rabbit mAb WB, IP, IHC-P H, M, R, Mk IHC analysis of paraffin-
embedded human lung
#9221 Phospho-ATF-2 (Thr71) Antibody WB, IP, IHC-P, IHC-F, IF-IC, F H, M, R, Mk
carcinoma using #3270.
#2250 c-Fos (9F6) Rabbit mAb WB, IF-IC, F, ChIP H, M, R, (Hm, B, Pg)
#5348 Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb WB, IP, IF-IC, F, ChIP H, M, R, (Hm, B, Pg, Hr)
#9165 c-Jun (60A8) Rabbit mAb WB, IP, IHC-P, IHC-F, IF-IC, ChIP H, M, R, Mk
#2361 Phospho-c-Jun (Ser63) (54B3) Rabbit mAb WB, IHC-P H, M, R
#3270 Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP H, M, R, Mk, Pg, (B)
#3058 DUSP6/MKP3 Antibody WB H
#3372 Erk5 Antibody WB, IP H, M, R, Mk
Phospho-p44/42
#3371 Phospho-Erk5 (Thr218/Tyr220) Antibody WB H, M, R, Mk MAPK (Erk1/2)
#9258 JNK2 (56G8) Rabbit mAb WB H, M, R, Hm, Mk, Mi (Thr202/Tyr204)
#3042 MAPKAPK-2 Antibody WB, IP, F H, M, R, Mk, (Hm) (D13.14.4E) XP®
Rabbit mAb #4370:
#3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Mk
Confocal IF analysis of
#2352 MEK1 (61B12) Mouse mAb WB, IP H, M, R, Mk C2C12 cells, treated
#9126 MEK1/2 (47E6) Rabbit mAb WB H, M, R, Mk, (Dg) with U0126 #9903
#9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb WB, IP H, M, R, Mk, Dm, (C) (10 μM, 1 hr; top) or
TPA #9905 (200 nM,
#4994 NIK Antibody WB H, M, (R, B, Hr) 15 min; bottom), using
#8690 p38 MAPK (D13E1) XP® Rabbit mAb WB, IHC-P, IF-IC, F H, M, R, Hm, Mk, Pg, (C) #4370 (green). Actin
#4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R, Mk, Mi, Pg, Sc, filaments were labeled
(Hm, C, Z, B, Pg) with Alexa Fluor® 555
Phalloidin #8953 (red).
#9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb WB, IP, IF-IC, F H, M, R, Mk, Sc, (Z)
Blue pseudocolor =
#4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb WB, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, Mi, DRAQ5® #4084 (fluo-
Dm, Z, B, Dg, Pg, Ce, (C) rescent DNA dye).
#4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) WB, IP, IHC-P, IF-IC, F H, M, R, Hm, Mk, Mi, Dm,
XP® Rabbit mAb Z, B, Dg, Pg, Sc, (C, Ce)
#9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) WB, F H, M, R, Hm, Mk, Mi, Dm,
Mouse mAb Z, B, Pg
#2708 p70 S6 Kinase (49D7) Rabbit mAb WB, IHC-P H, M, R, Mk
#9234 Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb WB H, M, R, Mk, (C)
#9433 B-Raf (55C6) Rabbit mAb WB H, M, R, Mk Phospho-p38 MAPK A kDa COS 293

#2696 Phospho-B-Raf (Ser445) Antibody WB H, M, R, Mk, (C, Dg) (Thr180/Tyr182) (D3F9) 100
80
XP Rabbit mAb #4511:
®
#9422 c-Raf Antibody WB H, M, R, Mk WB analysis (A) of extracts 60
50
#9421 Phospho-c-Raf (Ser259) Antibody WB, IP H, M, R, Mk, X, (C) from COS and 239 cells, 40
Phospho-
p38 MAPK
#9427 Phospho-c-Raf (Ser338) (56A6) Rabbit mAb WB H, M, R, Mk untreated (-) or UV-treated 30
(+) using #4511 (upper)
#9252 SAPK/JNK Antibody WB H, M, R, Hm, Mk, Z, B, Sc 20
or p38 MAPK Antibody
#4668 Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb WB, IP, IHC-P H, M, R, Dm, Sc #9212 (lower). Flow
10
#9255 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb WB, IP, IF-IC, F H, M, R, Hm, Sc cytometric analysis (B)
of Jurkat cells, untreated 100
#9152 SEK1/MKK4 Antibody WB, IP H, M, R, Mk, (Dg) 80
(blue) or treated with An-
#9156 Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody WB H, M, R, Dm, (X, Z) isomycin #2222 (green), 60
50
#5206 TAK1 (D94D7) Rabbit mAb WB, IP H, M, R using #4511 compared p38 MAPK
40
#9339 Phospho-TAK1 (Ser412) Antibody WB, IP H, M, R to a nonspecific negative 30
control antibody (red).
#8955 Ras (D2C1) Rabbit mAb WB H, M, R, Mk 20
#14429 Ras (G12D Mutant Specific) (D8H7) Rabbit mAb WB H, (M, R)
10
#14412 Ras (G12V Mutant Specific) (D2H12) Rabbit mAb WB H, (M, R) – + – + UV
#9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb WB, IP, IF-IC H, M, R, Mk B
#11989 Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb WB, IHC-P, IF-IC H, M, R, Mk, Mi,
(C, X, Z, B, Dg, Pg, Hr)

ELISA KITS
Events

#7274 PathScan® MAP Kinase Multi-Target Sandwich ELISA Kit

#7029 PathScan® Phospho-MEK1 (Ser217/221) Chemiluminescent Sandwich ELISA Kit
#7946 PathScan® Phospho-p38 MAPK (Thr180/Tyr182) Sandwich ELISA Kit
#7177 PathScan® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit
15FLYMAPK_REFM105ENG_00

#7849 PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Chemiluminescent Sandwich ELISA Kit Phospho-p38 MAPK (Thr180/Tyr182)

APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry
/ -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all
species expected / ( ) 100% sequence homology

© 2015 Cell Signaling Technology, Inc. Cell Signaling Technology, PathScan, and XP are trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners.

For Research Use Only. Not For Use In Diagnostic Procedures.

www.cellsignal.com/cstmapk