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Practical 3: To study the factors that affect the rate of enzyme reaction

Explore the Lactase Enzyme Simulation (virtual lab) using the following website:

https://sites.google.com/site/biologydarkow/lactase-enzyme-simulation

Aims:

1. To investigate the effect of various factors on the rate of enzyme reaction

2. To determine the best factor combination that produce the highest rate of enzyme
(lactase) reaction.

Write a report on the effect of each factor on the rate of lactase reaction. The report
should consist of the following:

 Aim
 Hypothesis
 Variables
 Procedure (refer to ‘Experimental Setup’ as a guide; in passive voice)
 Results (attach screenshot from the simulation)
 Discussion
 Conclusion

* You do not have to explore Vmax.

Answer all the ‘Questions to explore’

Write your reflections on what you learn in Practical 3 and the importance of enzyme.

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