IC A

Applica
ation No
ote U–5
56
Niitrite
e, broomidde an
nd niitrate
e in
arrtificial se
ea w
waterr app
plyingg
direct UV/V VIS d
detection
n

Seawwater analyysis with conductivity

c y detectionn is difficult due to tthe high excess
of cchloride. Esspecially analyzing
a ffor nitrite and brom mide, UV/V VIS detectio
on is
hloride is not interfer ing with nitrite at 218 nm. Thiss AN show
prefeerred as ch ws the
deteermination of all threee UV-absorrbing anion ns in an artificial seawwater.

Ressults
Peakk number Anion mg/L
m
1 Injectionn peak
2 Nitrite 0.08
0
3 Bromidee 0.18
0
4 Nitrate 3.40
3
 Me
ethod descript
d ion

Samp
ple
Artificiial seawater

Samp
ple preparation
Filtration through 0.2
2 μm, partial loo
op injection

mn
Colum

Metrrosep A Supp 10 - 250/4.0 6.1020.0330

Metrrosep A Supp 10 Guard/4.0 6.1020.5000

Solutiions

Eluen
nt: 5.0 mmool/L sodium
carbonate
5.0 mmool/L sodium
hydrogen
n carbonate
2.5% ace
etone

Analyysis

UV d
detection 218 nm

Param
meters

Flow
w rate 1.0 mL/m
min
Injecttion volume 5 μL
Pmax 20.0 MPa
a
ording time
Reco 28 min
Column temperaturre 50 °C

Instru
umentation

881 C
Compact IC pro
o – Anion – MC
CS 2.881.00030
887 Professional UV
V/VIS Detector 2.887.00010
www.meetrohm.com
IC Application Note U–56

858 Professional Sample Processorr 2.858.00020

Version 1