Online Enzyme Lab Simulation

Purpose/Background: In this investigation you will determine the effects of substrate concentration and pH on
the initial rate of an enzyme-catalyzed reaction. You will adjust the pH levels and the amount of substrate
added to collect the necessary data.

Lab URL: http://glencoe.mcgraw-hill.com/sites/0078695104/student_view0/unit2/chapter6/virtual_labs.html#

Question: How does pH affect reactions that are controlled by enzymes?

Hypothesis: ____________________________________________________________________________

Procedures:

Part 1
 Adjust the pH level by clicking the up or down arrows below each test tube to be set up as shown below
pH
Test Tube 1
3
Test Tube 2
5
Test Tube 3
7
Test Tube 4
9
Test Tube 5
11

 Add substrate, click and drag the .5g weighing paper with lactose on it (first paper) to each
test tube. What type of macromolecule is lactose? _____________________________

 Record your data in Table 1

Part 2
 Adjust the pH level by clicking the up or down arrows below each test tube to be set up as shown below
pH
Test Tube 1
3
Test Tube 2
5
Test Tube 3
7
Test Tube 4
9
Test Tube 5
11

 Add substrate, click and drag the 1g weighing paper with lactose on it (second paper)
to each test tube
 Record your data in Table 2
Part 3
 Adjust the pH level by clicking the up or down arrows below each test tube to be set up as shown below

pH
Test Tube 1
3
Test Tube 2
5
Test Tube 3
7
Test Tube 4
9
Test Tube 5
11

 Add substrate, click and drag the 2g weighing paper with lactose on it (third paper) to
each test tube
 Record your data in Table 3
Data/Analysis:
Data Table 1: pH Level with .5g substrate lactose (Blue Line)

Amount of Product Formed

pH 0.5 g
Test Tube 1
3
Test Tube 2
5
Test Tube 3
7
Test Tube 4
9
Test Tube 5
11

Data Table 2: pH Level with 1g substrate lactose (Red Line)

Amount of Product Formed

pH 1g
Test Tube 1
3
Test Tube 2
5
Test Tube 3
7
Test Tube 4
9
Test Tube 5
11

Data Table 3: pH Level with 2g substrate lactose (Green Line)

Amount of Product Formed

pH 2g
Test Tube 1
3
Test Tube 2
5
Test Tube 3
7
Test Tube 4
9
Test Tube 5
11
 Title
Y axis = Dependent Variable = ______________________________________

X axis = Independent Variable = ______________________________________

Conclusion/Summary: USE DATA FROM BOTH DATA TABLES AND BOTH GRAPHS TO SUPPORT ALL
ANSWERS. MAKE SPECIFIC REFERENCES TO YOUR DATA IN YOUR RESPONSES.WRITE IN
COMPLETE SENTENCES.

1. How did changing the pH affect the amount of product formed?

2. Explain how the amount of substrate affected the amount of product formed?

3. Was your hypothesis rejected or supported? Please explain and provide a summary of your overall
results.