TS SOP) ExiPrep 96lite Viral Kit 20200410

Revision 0 (2020-04-10)
ExiPrep TM 96 Lite SOP

(K-4614)
International Molecular Diagnosis TS Team
Copyright 2020. Bioneer Corporation. All Rights Reserved.

Revision 0 (2020-04-10)
General Work Flow

Using 70% EtOH and turn on the UV lamp.
Decontamination - Recommend every experiment.
- If contamination issue, clean up with 1% NaOCl together.
Can use various specimens, but some specimens need to pre-treatment process.
Pre-treatment - Sputum : Pre-treatment using E-Z solution.(BIONEER)
- Swab : If samples are sticky, do it.
Extraction Preparation
Extraction - Remove the sealing.
Preparation - Add proteinase K into buffer cartridge 1 and load 200μ of clinical sample.
- Set the buffer cartridge and select the appropriate protocol.
RNA Extraction Can apply Real-Time PCR

- You can use it to real-time PCR.
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Contents
01 Decontamination
02 Pre-treatment Process
03 Prepare the Nucleic Acid extraction
04 After Nucleic Acid Extraction

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01
Decontamination
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Decontamination
1. Turn on the instrument main switch.
2. Press the power button 2 seconds.
3. Open the door.
4. Wipe instrument inside (wall and elution block) using 70% EtOH.
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Decontamination
5. Touch upper of plate button.
6. Wipe the buffer cartridge layer 1 surface using 70% EtOH.
7. Touch upper of plate button again.
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Decontamination
12. Close the door.
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Decontamination
13. Touch the UV button and select 15 minutes. After 15min, automatically turn off.
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02
Pre-treatment Process
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Pre-treatment Process
Recommended sample type
• Sputum
• Swab (Nasopharyngeal swab, oropharyngeal swab)
→ If the sample is not too sticky, this process is not necessary.
Pre-treatment reagent
• NaOH (General)
• EZ solution (Only Bioneer, recommended)
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Pre-treatment Process - Sputum

1. Aliquot the EZ solution into conical tubes.
2. Add 1000µl(1ml) of the EZ solution into sample to reduce the viscosity and inhibitors.
And mix with vortex mixer.
(Recommend to add 1:1 ratio volume of EZ solution. However, because it is difficult to
accurately measure the volume of sputum, add 1ml.)
Wait 20 minutes
Caution!
Repeat the process if
※ For detailed procedures, refer to pre-treatment guide made by Bioneer. sample is still viscous.
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Pre-treatment Process - Swab

1. If the sample on the swab has high viscosity even in the transport medium, add the
same amount of Resuspension Buffer or 1XPBS, normal saline, etc.
2. Transfer 500 ㎕ of sample to 1.5 ㎖ micro-centrifuge tube and spin down for 5 seconds
at 6,000 rpm. (2,500 x g)
3. Take the supernatant 400 ㎕ and use it for extraction.
※ For detailed procedures, refer to pre-treatment guide made by Bioneer.

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03
Prepare the Nucleic Acid Extraction
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# Component Quantities
1 Buffer Cartridge ① 4 ea
2 Buffer Cartridge ② 4 ea
3 Buffer Cartridge ③ 4 ea
4 Buffer Cartridge ④ 4 ea
5 Buffer Cartridge ⑤ 4 ea
6 Buffer Cartridge ⑥ 4 ea
7 Buffer Cartridge ⑦ 4 ea
8 Magnetic Rod Cover 12 ea
9 Sealing Film 8 ea
10 Proteinase K 8 ea
11 User’s Guide 1 ea
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1. Load 1250μl of DEPC DW into proteinase K tube and vortex.
※ After use, keep the proteinase K in 4℃.
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2. Remove the sealing film of all buffer Cartridges. (Total Seven types)
3. Then close the acryl lids.
Caution!
Please be careful the buffer splattered or
flow other cartridge wells.
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4. Load the 20μl proteinase K into buffer cartridge 1.
5. Close the buffer cartridge 1 acryl lids after loading is complete.
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6. Transfer buffer cartridge 1 to the biosafety cabinet. (Class 2 or 3)
※ All related works should be conducted inside negative pressure biosafety cabinet. (Class 2 or 3)
- De-capping the tube

- Pipetting
- Capping of clinical samples
- Capping containers
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7. Load the 200μl of pre-treated sample into buffer cartridge 1.
8. Close the acryl lids.
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9. Transfer all buffer cartridge to ExiPrepTM 96 lite.
10. Open the door and touch upper of plate button.
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10. Attach the magnetic rod cover until it clicks.
11. Load the buffer cartridge 1 and 2 on layer 1. And remove the cover.
Push
2nd Cartridge
1st Cartridge
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12. Touch the upper of plate button again.
13. Load the buffer cartridge 3 and 4 on layer 2.
14. Remove the cover.
4th Cartridge
3rd Cartridge
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16. Load the buffer cartridge 5 and 6 on layer 3.
6th Cartridge
5th Cartridge
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19. Load elution cartridge (buffer cartridge 7) on elution heating block.
7th Cartridge
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21. Close the door.
22. If you want to input the sample information, touch the assign sample. (But you can skip it.)
23. Touch the standard protocol.
24. Select the K-4614. (Prep kit Cat. No.)
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25. Touch the play button.
26. One more check the buffer cartridge cover. And touch the √ in the check list.
Then 96Lite will start.
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04
After Nucleic Acid Extraction
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After Nucleic Acid Extraction

After complete the preparation,
1. Open the door and take off the elution cartridge (7th cartridge). And close the lid.
2. You can use it to real time PCR.
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After Nucleic Acid Extraction_Cleaning

4. Remove the magnetic rod cover.
5. Close the lid and remove the buffer cartridge 1 and 2.
6. Wipe the surface using 70% EtOH.
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8. Close the lid
9. Remove the buffer cartridge 3 and 4.
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12. Close the lid
13. Remove the buffer cartridge 5 and 6.
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16. Close the door
17. Touch the UV button and select 15 minutes. After 15min, automatically turn off.
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THANK YOU

TS SOP) ExiPrep 96lite Viral Kit 20200410

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TS SOP) ExiPrep 96lite Viral Kit 20200410

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Revision 0 (2020-04-10)

ExiPrep TM 96 Lite SOP

International Molecular Diagnosis TS Team

Copyright 2020. Bioneer Corporation. All Rights Reserved.

General Work Flow

RNA Extraction Can apply Real-Time PCR

03 Prepare the Nucleic Acid extraction

04 After Nucleic Acid Extraction

Pre-treatment Process - Sputum

Pre-treatment Process - Swab

same amount of Resuspension Buffer or 1XPBS, normal saline, etc.

at 6,000 rpm. (2,500 x g)

3. Take the supernatant 400 ㎕ and use it for extraction.

※ For detailed procedures, refer to pre-treatment guide made by Bioneer.

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

- De-capping the tube

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

Prepare the Nucleic Acid Extraction

After Nucleic Acid Extraction

After Nucleic Acid Extraction_Cleaning

After Nucleic Acid Extraction_Cleaning

After Nucleic Acid Extraction_Cleaning

After Nucleic Acid Extraction_Cleaning

Copyright 2020. Bioneer Corporation. All Rights Reserved.

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