pGlo

Lab Report 20 points

1. Using a permanent marker on the petri dish, mark and count the number of individual colonies. (Use

the photographs of your petri dishes to mark and count colonies.) If the cell growth is too dense to

count individual colonies, record “lawn”. (4 points)

Plate #1 Plate # 2

iii

iii

LB -pGlo (positive control) LB/amp +pGlo (experimental)

Lawn 2
Number of colonies or lawn = _________ Number of colonies or lawn = ________

Plate #3 Plate #4

air s

bubbles II

in the

agar plate

LB/amp/ara +pGlo (experimental) LB/amp, -pGlo (negative control)

0
3
Number of colonies or lawn = ________ Number of colonies or lawn = ________

2. Based on your observed results, write a brief explanation of what happened in each of the 4 plates -

did bacteria grow and, if so, how much? What color were the colonies? (4 points)

Plate Brief explanation, including qualitative and quantitative observations

number

1
I observed that bacteria grew

into a lawn all over the agar

plate in a yellow color.

I observed that the bacteria barely grew.

There were two white spots in the plate.

3 I observed that the bacteria was able to

grow in the plate. There was three

colonies of bacteria with a yellow color.

4
I observed that the bacteria did not grow in

this plate. But there were 12 air bubbles.

3. Which plates had bacteria that were treated with the pGlo plasmid? (1 point)

Plate two had the plasmid, Luria Broth,

and ampicillin. Plate three had the plasmid,

Luria Broth, ampicillin, and aribinose.

4. What evidence would you look for to determine if the bacteria were successfully transformed? (2

points)

If the bacteria changes color to white instead

of yellow or glows under a fluorescent light, the

bacteria was successfully transformed.

5. Why was it important to have two controls in this experiment? Explain the scientific reasoning of

having a positive control and a negative control. (4 points)

It was important to have two

controls because the positive

showed us that if there is no plasmid

and plasmid the bacteria grows

uncontrollably. The negative control

showed us that with no plasmid with

ampicillin there is no growth at all.

This helps us add another variable to

experiment the effects it has on the

bacteria. A positive control shows a 2

result while a negative does not.

They are expected results that give a

comparison for thee experiment.

6. Conclusion: Which petri dish (or dishes) contained transformed bacteria? Include supporting

evidence and also discuss how you used the control plates to confirm your conclusion. (5 points)

The cells in plate three glowed a green color under a

fluorescent light due the plasmid and arabinose. The

colonies on plate two appeared white in color rather

than yellow so the bacteria underwent a

transformation due to the plasmid and ampicillin.

Plate two I used the the negative control plate to

confirm my conclusion because the added plasmid
was the only difference. For plate three I used plate
two as a comparison because it only added the
arabinose which proves the arabinose made the
bacteria glow because plate two did not glow and
plate three did.