CD1700 / 1800 references

CD1700/1800 references:
WBC RBC HGB MCV PLT MPV
Background Count Open <0.5 <0.05 <0.1 <10.0
Carryover <1.0 <0.5 <0.8 <1.0
Open Precision <2.5% <1.7% <1.2% <1.5% <6.0% <3,8.0%
Closed Precision <2.7% <1.7% <1.2% <1.5% <6.0%
Open Mode Cal Ver Diff <0.2 <0.06 <0.2 <1.0 <10.0
*Closed mode cal ver <2.7% <1.7% <1.2% <1.5% <6.0%
**Closed mode cal ver <2.7% <1.7% <1.2% <1.5% <6.0%

RAW FILE:
Parameter: UP TIME UP TIME CNT- TIME CNT- TIME
HGB Reference : 1850-2250 NA NA NA NA
5,0 +- 0,2V
HGB Sample : +- 10 NA NA NA NA
WBC : NA 1,8-2,2 2.0 4,5-5,5 5.000
RBC : NA 4,8-5,2 5.0 6,5-6,9 6.700
Lyse Volume:
CD1600 for normal Calibration set Lyse Volume to 1,0 ml, CD1700 /1800, the Lyse Volume is fixed to 1,0 ml.

New Factor:
Reference ( target )
new factor = -------------------------- * current Factor
Value found
MPV Cal factor:
v = 4/3 PHI * r3 = 4,187 * r3 , v = ( d3 * PHI) / 6 = 0,5233 d3 ;
for 3,3µm diameter the MPV is = 18,8058321 fl, 18,8 fl
Electronic Differential Thresholds – Comparison Chart
CELL-DYN 1600 /1700/ 1800 WBC Channels and Algorithms:
Cell Group CD1600 CD1600 CD1700 CD1700 CD1800 CD1800
Channels Volume Channel Volume Channels Volume
s
G0 1-20 0 – 27 fL 1 – 15 0 – 27 fL 1 – 11 0 – 20 fL
G1 21-25 28 – 34 fL 16 - 19 28 – 34 fL 12 - 19 21 – 34 fL
Lymphs G2 26 – 31 35 – 42 fL 20 – 25 35 – 44 fL 20 – 30 35 – 53 fL
G3 32 – 59 43 – 81 fL 26 – 49 45 – 87 fL 31 – 42 54 – 74 fL
G4 60 - 72 82 – 98 fL 50 - 62 88 – 109 fL 43 - 55 75 – 97 fL
Mid G5 73 – 89 99 – 120 fL 63 – 83 110 – 146 fL 56 – 69 98 – 121 fL
Cells G6 89 - 98 121 – 134 fL 84 - 96 147 – 169 fL 70 - 89 122 – 156 fL
Grans G7 99 – 109 135 – 149 fL 97 – 109 170 – 192 fL 90 – 109 157 – 192 fL
G8 110 – 228 150 – 312 fL 110 – 228 193 – 401 fL 110 – 228 193 – 401 fL
G9 229 – 253 313 – 346 fL 229 – 253 402 – 445 fL 229 – 253 402 – 445 fL
G10 254 – 255 347 – 349 fL 254 – 255 446 – 449 fL 254 – 255 446 – 449 fL
G11 256 350 fL 256 450 fL 256 450 fL

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 CD1700 / 1800 references

CELL-DYN 1600 Region CELL-DYN 1700 Region CELL-DYN 1800 Region

Alert Algorithms Alert Algorithms Alert Algorithms
R0 Group [1] ≥ 200 Group [1] ≥ 135 Group [1] ≥ 300
LYM R1 Group [2] / LYM # ≥ 0.20 Group [2] / LYM ≥ 0.20 Group [2] / LYM ≥ 0.35
LYM R2 Group [4] / LYM # ≥ 0.3 Group [4] / LYM ≥ 0.192 Group [4] / LYM ≥ 0.40
MID R2 Group [5] / Group [4] ≥ 1.5 Group [5] / Group [4] ≥ 1.75 Group [5] / Group [4] ≥ 1.3
MID R3 Group [7] / Group [6] ≥ 2.20 Group [7] / Group [6] ≥ 2.20 Group [7] / Group [6] ≥ 2.5
GRAN Group [7] / GRAN # ≥ 0.07 Group [7] / GRAN ≥ 0.061 Group [7] / GRAN ≥ 0.18
R3 or
Group [7] / Group [6] > 1,5
R4 NA Group [10] / Group [9] ≥ Group [10] / Group [9] ≥
0.110 0.14
or or
Group [10] / Group [11] / Group [10] + Group [11] /
Group [9] ≥ 1.64 Group [9] ≥ 1.8

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 CD1700 / 1800 references

Parameter: Target Value CD1600 MAM Target Value CD1700 MAM Target Value CD1800
adjustment adjustment SPM adjustments
WBC Latex 5,0 µ @ CH 71+- 1 Latex 5,0 µ @ CH 56 +-1 Latex 5,0 µ @ CH 56 +-1
R 4, CW is + direction R 4, CW is + direction R 22, CW is - direction
WBC 0 to 350 fL 0 to 450 fL 0 to 450 fL
Histogram 1,367 fl / CH 1,758 fl / CH 1,76 fl / CH
RBC Latex 5,0 µ @ CH 98 +- 1 Latex 5,0 µ @ CH 98 +- 1 Latex 5,0 µ @ CH 98 +- 1
1fl /CH 1fl /CH 1fl /CH
MCV, Dec 98, MCV, Dec 98, R 27, CW is - direction
R 9, CW is + direction R 9, CW is + direction
CNT Dec 101, CNT Dec 101,
R 8, CW is + direction R 8, CW is + direction
MCV RER, Latex 5,0 µ @ CH 98 +- RER, Latex 5,0 µ @ CH 98 +- 1 RER, Latex 5,0 µ @ CH 98 +-
1 R 72 ,CW is + direction 1 R 51 ,CW is - direction
R 72, CW is + direction
PLT Latex 3,3 µ @ CH 136 +- 1 Latex 3,3 µ @ CH 136 +- 1 Latex 3,3 µ @ CH 136 +- 1
0,1367 fl /CH 0,1367 fl /CH 0,1367 fl /CH
R 15, CW is + direction R 15, CW is + direction R 41, CW is - direction

Electronic Differential Thresholds – Comparison Chart

CELL-DYN 1600 /1700/ 1800 WBC Channels and Algorithms:

Cell Group CD1600 CD1600 CD1700 CD1700 CD1800 CD1800
Channel Volume Channel Volume Channel Volume
s s s
G0 1-20 0 – 27 fL 1 – 15 0 – 27 fL 1 – 11 0 – 20 fL
G1 21-25 28 – 34 fL 16 - 19 28 – 34 fL 12 - 19 21 – 34 fL
Lymphs G2 26 – 31 35 – 42 fL 20 – 25 35 – 44 fL 20 – 30 35 – 53 fL
G3 32 – 59 43 – 81 fL 26 – 49 45 – 87 fL 31 – 42 54 – 74 fL
G4 60 - 72 82 – 98 fL 50 - 62 88 – 109 fL 43 - 55 75 – 97 fL
Mid G5 73 – 89 99 – 120 fL 63 – 83 110 – 146 fL 56 – 69 98 – 121 fL
Cells G6 89 - 98 121 – 134 fL 84 - 96 147 – 169 fL 70 - 89 122 – 156 fL
Grans G7 99 – 109 135 – 149 fL 97 – 109 170 – 192 fL 90 – 109 157 – 192 fL
G8 110 – 228 150 – 312 fL 110 – 228 193 – 401 fL 110 – 228 193 – 401 fL
G9 229 – 253 313 – 346 fL 229 – 253 402 – 445 fL 229 – 253 402 – 445 fL
G10 254 – 255 347 – 349 fL 254 – 255 446 – 449 fL 254 – 255 446 – 449 fL
G11 256 350 fL 256 450 fL 256 450 fL

CELL-DYN 1600 Region CELL-DYN 1700 Region CELL-DYN 1800 Region

Alert Algorithms Alert Algorithms Alert Algorithms
R0 Group [1] ≥ 200 Group [1] ≥ 135 Group [1] ≥ 300
LYM R1 Group [2] / LYM # ≥ 0.20 Group [2] / LYM ≥ 0.20 Group [2] / LYM ≥ 0.35
LYM R2 Group [4] / LYM # ≥ 0.3 Group [4] / LYM ≥ 0.192 Group [4] / LYM ≥ 0.40
MID R2 Group [5] / Group [4] ≥ 1.5 Group [5] / Group [4] ≥ 1.75 Group [5] / Group [4] ≥ 1.3
MID R3 Group [7] / Group [6] ≥ 2.20 Group [7] / Group [6] ≥ 2.20 Group [7] / Group [6] ≥ 2.5
GRAN R3 Group [7] / GRAN # ≥ 0.07 Group [7] / GRAN ≥ 0.061 Group [7] / GRAN ≥ 0.18
or
Group [7] / Group [6] > 1,5
R4 NA Group [10] / Group [9] ≥ Group [10] / Group [9] ≥
0.110 0.14
or or
Group [10] / Group [11] / Group [10] + Group [11] /
Group [9] ≥ 1.64 Group [9] ≥ 1.8

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 CD1700 / 1800 references

Sample 30µl

Pre-Mix Dilution 1:251 (30µl spl + 7.5ml Dil)

WBC/HGB Dilution RBC/PLT Dilution

7.4ml from Pre-Mix cup + 1ml Lyse 0.1ml from Pre-Mix cup + 5ml Diluent
1:284 1:12801

WBC HGB RBC PLT

(MCV, MPV)

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 CD1700 / 1800 references

Use XB indices focus first on RBC # and HGB, most stable parameter.
MCV is depending on Oxygenation and Diluent Osmolarity!
Use MCHC as indicator in which way to correct MCV!

Specifications:
WBC RBC HGB MCV PLT MPV
Background Count Open <0.5 <0.05 <0.1 <10.0
Carryover <1.0 <0.5 <0.8 <1.0
Open Precision <2.5% <1.7% <1.2% <1.5% <6.0% <3,8.0%
Closed Precision <2.7% <1.7% <1.2% <1.5% <6.0%
Open Mode Cal Ver Diff <0.2 <0.06 <0.2 <1.0 <10.0
*Closed mode cal ver <2.7% <1.7% <1.2% <1.5% <6.0%
**Closed mode cal ver <2.7% <1.7% <1.2% <1.5% <6.0%

Service DEC Code 128

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