Application

Determination of Sugar Solution Color

at pH 7.0 (According to ICUMSA
Method GS2/3-9)
Method: Spectrophotometric Color Determination at 420 nm

Materials and Reagents:

Cat.No. 1.16754.9010 Water for analysis EMSURE® or distilled water
Cat.No. 1.09060.1000 Hydrochloric acid 0.1 mol/l Titripur®
Cat.No. 1.08379.0250 Triethanolamine (TEA) for analysis

Cat.No. 1.14944.0001 Rectangular cells 50 mm or

Cat.No. 1.74011.0001 Rectangular cell 100 mm (Prove 600 only)

Cat.No. 1.73016.0001 Spectroquant® VIS Spectrophotometer Prove 100 or

Cat.No. 1.73017.0001 Spectroquant® UV/VIS Spectrophotometer Prove 300 or
Cat.No. 1.73018.0001 Spectroquant® UV/VIS Spectrophotometer Prove 600

Membrane filters, cellulose nitrate, pore size 0.45 µm, diameter 50 mm

Conical flasks 250 ml
Volumetric flask 500 ml
Beaker 1000 ml
pH meter
Ultrasonic bath
Refractometer

Method:
Spectrophotometric Color Determination at 420 nm

Measuring range:
Method No. 2549
0 – 600 IU7.0

Working instructions:
TEA buffer solution
Dissolve 7.46 g of TEA in 200 ml water (Water for analysis EMSURE® is recommended), transfer to a 500 ml
volumetric flask and make up to the mark with water. Transfer the 500 ml to a beaker and adjust the solution to
pH 7.0 with 0.1 mol/l Hydrochloric acid Titripur® (approx. 420 ml of hydrochloric acid solution are required).

Sample preparation
Mix the sample thoroughly.
Weigh 50.0 ± 0.1g of the sample into a 250 ml conical flask.
Add 50.0 ± 0.1g of the prepared TEA buffer solution and dissolve the sample by swirling at room temperature.
Vacuum-filter the pretreated solution over a membrane filter into a clean and dry conical flask.
Degas the solution for 3 minutes in the ultrasonic bath.

Measure the % RDS (refractometric dry substance) of the solution to an accuracy of ± 0.1 g/100g (according to
ICUMSA Method GS4/3-13).

Merck KGaA, 64271 Darmstadt, Germany, Tel. 2017-02-14 Page 1 of 2

+49(0)6151 72-2440
EMD Millipore Corporation, 290 Concord Road,
Billerica, MA 01821, USA, +1-978-715-4321
Application

Zero adjustment
For each measurement series, a zero adjustment is required.
It is recommended to use the same cell for zero adjustment and for sample measurement.
The zeroing procedure for the measurement series is automatically prompted by the instrument after entering the
%RDS value.
For zero adjustment fill a corresponding rectangular cell with TEA buffer solution.
After prompting, place the rectangular cell filled with buffer solution in the cell compartment, the zero adjustment
is executed automatically.
Confirm the implementation of zero adjustment with OK.
The zero adjustment is valid for the entire measurement series.

Measurement procedure
Select the method (2549, "ICUMSA Color GS2/3-9") from the method list of the Spectroquant® Prove.
Enter the RDS value in %. Confirm with OK.
Press START to start the measurement mode.
Perform a zero adjustment (procedure see “Zero adjustment”) with TEA buffer solution.
Transfer the sample solution into a corresponding cell. Place the cell into the cell compartment. The measurement
is performed automatically.
Confirm measurement with OK. The IU7.0 value is shown in the display.

Press START to start the measurement procedure for the next sample. The system does not prompt a repeat of
the zero adjustment.

Note:
The result is calculated according to ICUMSA GS2/3-9(2005) chapter 8.1 and ICUMSA SPS-4 table B (Sucrose;
20°C).
Individual ICUMSA methods or references are available online within 48 h: www.icumsa.org under “Methods
book”.

Short measuring procedures on Spectroquant® Prove instruments are described in the Analytical Procedures and
Appendices, available online on our website.

Ordering Information
Product Catalog No.
Prove 100 VIS Spectrophotometer 4 nm spectral bandwidth Spectroquant® 173016
Prove 300 UV/VIS spectrophotometer 4 nm spectral bandwidth Spectroquant® 173017
Prove 600 UV/VIS spectrophotometer 1,8 nm spectral bandwidth Spectroquant® 173018
Water for analysis EMSURE® 116754
Polypropylene Adapter 109060
Triethanolamine GR for analysis 108379
Rectangular cells 50 mm Spectroquant® 114944
Rectangular cell 100 mm for Prove 600 Spectroquant® 174011

Merck KGaA, 64271 Darmstadt, Germany, Tel. 2017-02-14 Page 2 of 2

+49(0)6151 72-2440
EMD Millipore Corporation, 290 Concord Road,
Billerica, MA 01821, USA, +1-978-715-4321