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  • 8A Enzyme Data Sheets

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    Alyanna Julia Vibal
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    This document describes several tests conducted to analyze enzyme activity and specificity. A Biuret test was performed to detect proteins using sodium hydroxide and copper sulfate. A catalase test used hydrogen peroxide and a wood splint to observe oxygen gas production over time. Two tubes with different substrates were observed over 30 minutes to demonstrate the specific action of salivary amylase on starch.

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    This document describes several tests conducted to analyze enzyme activity and specificity. A Biuret test was performed to detect proteins using sodium hydroxide and copper sulfate. A catalase test used hydrogen peroxide and a wood splint to observe oxygen gas production over time. Two tubes with different substrates were observed over 30 minutes to demonstrate the specific action of salivary amylase on starch.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    21 views2 pages

    8A Enzyme Data Sheets

    Uploaded by

    Alyanna Julia Vibal
    This document describes several tests conducted to analyze enzyme activity and specificity. A Biuret test was performed to detect proteins using sodium hydroxide and copper sulfate. A catalase test used hydrogen peroxide and a wood splint to observe oxygen gas production over time. Two tubes with different substrates were observed over 30 minutes to demonstrate the specific action of salivary amylase on starch.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    of 2

    ENZYMES

    DATA AND RESULTS:

    BIURET TEST OBSERVATION

    Extract + 10% NaOH + CuSO4

    TEST FOR CATALASE ACTIVITY OBSERVATION

    Extract + 3% H2O2 wood


    splint

    Add 1 ml 0.5% Benzidine

    TEST FOR SPECIFICITY OF OBSERVATION


    ENZYME ACTION

    Test tube no. 1

    5 minutes

    10 minutes

    15 minutes

    20 minutes

    25 minutes

    30 minutes

    TEST FOR SPECIFICITY OF OBSERVATION


    ENZYME ACTION

    Test tube no. 2

    5 minutes

    10 minutes

    15 minutes

    20 minutes
    25 minutes

    30 minutes

    Questions:
    1. What principle is involved in the Biuret’s test?
    2. How will you prove the presence of catalase. Show the chemical equation.
    3. How will you prove the specific action of salivary amylase?
    .

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