Differentiation of Enterobacteriaceae with a KIA/TSIA reaction of Alkaline/Acid, With/Without Gas

Citobacter diversus
Shigella Proteus Providencia Serratia Enterobacter Yersinia

Anaerogenic E. coli
S. paratyphi A2

agglomerans
alcalifaciens

pseudotuter
dysenteriae

marcescens
morganii
liquefaciens hafniae

rettgeri
flexneri

stuartii
sonnei
Testing Media

boydii

pestis
37oC 22oC 37oC 22oC
H2S (KIA/TSIA)…………………. V - - - - - - - - - - - - - - - - V- -
Carbohydrate Fermentations
Glucose and Gas………………… A A A A A A A A A A A A A A A A A A A
Lactose…………………………….. - - - - A6 - - - - - V-6 V- V V- V- V V - -
Sucrose…………………………….. - - - - A6 - V-6 V- V6 V A A A V- V- V V- - -
Manitol………….…………………. A - A7 A A6 - V - V- A A A A A A A A A A
Dulcitol……………………………. V+ V- - V - - - - - - - - - - V V - A
Salicin………………………………. - - - - - - V - - V A A A V- V- V V-6 V+ A
Adonitol…………………………… - - - - - - V+ A - V V V- V- - - - A - A
Inositol……………….……………. - - - - - - A - A - V- V V - - V- - - -
Sorbitol……………………………. A - - - - - - - V- - A A A - - - A V -
Arabinose………...………………. A - - - - - - - - A - A A A A A A A A
Raffinose………………………….. - - - - - - - - - A - A A - - V - - -
Rhammose……………………….. A - - - - - V - - V - V- V- A A V+6 A V- A
Melibiose………………………….. A - - - - - - - - V - V+ NR - NR NR NR V A
Trehalose…………………………. A NR NR NR NR - V - V A A A A A A A NR A A
Xylose………………………………. - - - V V - V - - A - V V A A A NR A -
Amygdalin………………………… - - - - - - - - - V NR NR NR - NR NR NR NR NR
IMViC Indole…………………….. - V V V - + + + + - - - - - - V + - -
Methyl Red……………………….. + + + + + + + + + + V V+ V- V+ - V + + +
Voges Proskaurer……………… - - - - - - - - - - + V- V+ V + V - - -
Simmons Citrate………………… - - - - - - + + + - + + + V- V V + - -
Malanote…………………………… - V V V V - -
Gloconate………………………….. - - - - - - - - - - + + + + + NR NR - -
Chistensen’s Urease…………… - - - - - + + - - - Vw Vw - - - V+ V+ - +
Motility……………………………… + - - - - V+ + + V+ - + + + + + V+ + - +
Gelation Liquofaction 22oC… - - - - - - - - - - V+6 - + - V- - - -
KCN broth-growth……..…...… - - - - - + + + + - + + + + + V- - - -
Arginin dihydrolase NH3……… + - - V- V - - - - V- - - - V- - - V6 - -
Lysine Decarboxylase……….. - - - - - - - - - V+ + V V + + - - - -
Ornithine Decarboxylase…… + - - - + + - - - V- + + + + + - + - -
Phenylalanine Deaminase…. - - - - - + V+8 + + - - - - - - V - - -
ONPG……………………………….. - V+ V+ V+ V+ - - - - - + + + + + NR V+ + +
Esculin……………………………… - - - - - - V - - NR + NR NR - NR V NR + +
4 7
1
Organisme listed all possible from either a KIA/TSIA reaction of alkaline/acid Gas production variable, mojority positive Shigella flexneri, serotype 6 may be mannitol negative
2 5 8
Salmonella enteretidis bioserotype parathypi Gas production variable, majority negative Rapid
3 6
Shigella flexneri, serotype 6 gasproduction positive may be delayed
 Differentiation of Enterobacter with a KIA/TSIA reaction of Acid/Acid, With/Without Gas

Enterobacter Serratia

Eschericia coli

Klebsiella

agglomerans

Yersinia
marcescens
aerogenes

rubidaea
hafniae liquefaciens

concae
Testing Media

37oC 22oC 37oC 22oC

H2S (KIA/TSIA)…………………. - -2 - - -3 - - - - - - -
Carbohydrate Fermentations
Glucose and Gas………………… A A A A A A A A A A A A
Lactose…………………………….. A4 A V+4 A V- V- V V-4 V- V A -
Sucrose…………………………….. V A3 A A V- V- V A A A A A
Manitol………….…………………. A A A A A A A A A A A A
Dulcitol……………………………. V V- V- - - - V- - - - - -
Salicin………………………………. V A V4 A V- V- V A A A V-4
Adonitol…………………………… - V+ V- A - - - V V- V- V-
Inositol……………….……………. - A V- A - - V- V- V- V V-
Sorbitol……………………………. V+ A A A - - V- A A A - A
Arabinose………...………………. A A A A A A A - A A A V
Raffinose………………………….. V A A A - - V - A A A -
Rhammose……………………….. V+ A V+ A A A V-4 - V- V- - -
Melibiose………………………….. A A A A - NR NR - V+ NR A -
Trehalose…………………………. A A A A A A A A A A A A
Xylose………………………………. V A A A A A A - V V V V
Amygdalin………………………… - A A A - NR NR NR NR NR NR NR
IMViC Indole…………………….. + V- - - - - V - - - V-
Methyl Red……………………….. + V- - - V+ - V V V+ V- V
Voges Proskaurer……………… - + + + V + V + V- V- +
Simmons Citrate………………… - + + + V- V V + + + +
Malanote…………………………… - + V+ V V V V - - - V-
Gloconate………………………….. - + + + + + NR + + + NR NR
Chistensen’s Urease…………… - + V- - - - Vw Vw Vw - Vw V
Motility……………………………. V- - + + + + V+ + + + V+
Gelation Liquofaction 22oC… - - V- V - V- V+ + Vw
KCN broth-growth……..…...…. - + + + + + V- + + + V+
Arginin dihydrolase NH3……… V - + - V- - - - - - -
Lysine Decarboxylase……….. V- + - + + + - + V V V4
Ornithine Decarboxylase…… V - + + + + - + + + -
Phenylalanine Deaminase….. - - - - - _ V - - - -
ONPG……………………………….. + + + - + + NR + + + NR
Esculin……………………………… V + V- + - NR V + NR NR NR
Coloni pigmentation………….. - - V+3 - - - + V - - -
1
possible organisme KIA : Escherichia, Klebsiella or Enterobacter spp; TSIA : Eschericia, Klebsiella, Enterobacter, Serratia or Yersiniaenterocolitica
2
Differentation of three species of Klebsiella 5
Yellow, nondiffused
3
Negative H S on KIA or TSIA but may be weakly peptone leon agar medium
2 6
Non water solute , pink red magenta pigmen temperature
4
may be delayed (220C), rerely at (37oC) slow.
Differentation of Enterobacteriaceae with a KIA/TSIA reaction of Alkaline/Acid, H2S, With/Without Gas

Proteus

Other Salmonella spp2

Citobacter freundii
Arizona hinshawii
Salmonella thypi

mirabilis

vulgaris
Testing Media

H2S (KIA/TSIA)………………………………. + 3 V+ + V+ + +
Carbohydrate Fermentations
Glucose and Gas…………………………….. A A A4 A A A3 A3
Lactose…………………………………………. - - - V+6 V6 V-6 -
Sucrose…………………………………………. V- - - V- V- - A
Manitol………….……………………………… - A A A A - -
Dulcitol………………………………………… - - V7 - V V-6 -
Salicin…………………………………………… - - - - V -
- V
Adonitol……………………………………….. - - - - - - -
Inositol……………….……………………….. - - V -
- - - -
Sorbitol………………………………………… - - A A A - -
Arabinose………...…………………………… V -
- V +6
A A - -
Raffinose………………………………………. - - - - V- - -
Rhammose……………………………………. - - A A A - -
Melibiose……………………………………… - - - A A - -
Trehalose……………………………………… - A V NR A A V
Xylose………………………………………….. - V A NR A A V
Amygdalin……………………………………. - NR NR - A - A
IMViC Indole…………………………………. + - - - V- - +
Methyl Red……………………………………. + + + + + + +
Voges Proskaurer………………………….. - - - - - V- -
Simmons Citrate……………………………. - - V +
+ + V 3
V-
Malanote………………………………………. - - - + V- - -
Gloconate……………………………………… - - - - - V -
Chistensen’s Urease……………………….. - - - - Vw V+ +
Motility………………………………………… + + V +8
+ + + +
Gelation Liquofaction 22oC…………….. - - - V6 - + +
KCN broth-growth……..…...……………… - - - - + + +
Arginin dihydrolase NH3……………………….. - + V+6 V-6 V - -
Lysine Decarboxylase……………………. + + + + - - -
Ornithine Decarboxylase……………….. + - + + V- + -
Phenylalanine Deaminase……...………. - - - - - + +
ONPG…………………………………………… - - - + V- - -
Esculin…………………………………………. - - - NR NR - V
ithout Gas
 Differentiation of Enterobacteriaceae with a KIA/TSIA raction of Acid/Acid, Gas, H 2S

Citrobacter Arizona
Testing Media freundii hinshawii Proteus

H2S (KIA/TSIA)……………..........
V+ + + +
Carbohydrate Fermentations
Glucose and gas…………............. A A A A
Lactose……………………............... V2 V+3 - -
Sucrose……………………………… V- V- V-3 A
Manitol………………………............ A A - -
Dulcitol……………………………… V - - -
Salicin………………………………... V- - V-3 V
Adonitol…………………………….. - - - -
Inositol…………………………….... - - - -
Sorbitol……………………………… A A - -
Arabinose…………………………... A A - -
Raffinose……………………………. V- - - -
Rhammose…………………………. A A - -
Melibiose…………………………… A A - -
Trehalose…………………………… A NR A V
Xylose………………………………... A NR A V
Maltose……………………………… A NR - A
Amygdalin………………………….. A - - A
IMViC Indole…………………….... V- - - +
Methyl Red………………………… + + + +
Voges Proskaurer……………….. - - V- -
Simmons Citrate……………….... + + V3 V
Malanote……………………………. V- + - -
Gloconate…………………………… - - V -
Chistensen’s Urease……………. V - V+ +
Motility……………………………… + + + +
Gelation Liquofaction 22oC.… - V3 + +
KCN broth-growth……………… + - + +
Arginin dihydrolase NH3…...... V V-3 - -
Lysine Decarboxylase…………. - + - -
Ornithine Decarboxylase……. V- + + -
Phenylalanine Deaminase…… - - + +

1
Possible organism KIA : Citrobacter freundii; TSIA : Citobacter freundii, Arizona, P. mirabilis and P. vulgaris
2 3
If gas produced, a alight amount May be delayed
Differentiation of the lost frequently isolated non enteric, intestinal bacteria
with KIA reaction of Alkaline/No change

Acinetobacter Alkaligenes Pseudomonas

Testing Media
calcoaceticus faecalis aeruginosa

Catalase…………………………………. 2 + +
Oxidase………………………………….. -2 + +
Nitrate reduction……………………. - V+ +
O-F Glucose……………………………. O/-3 -8 O
KIA/TSIA reaction………………….. ALK/NC ALK/ALK ALK/ALK
H2S (KIA/TSIA)………………………. - - -
Carbohydrate fermentations
Glucose and Gas……………………… A(O)4 - - A (O)
Lactose………………………………….. - - -
Sucrose………………………………...... - - -
Mannitol……………………………….... V4 - - V (O)
Dulcytol………………………………….. - - -
Salicin…………………………………….. - - -
IMViC
Indole…………………………………...... - - -
Methyl Red……………………………... - - -
Voges-Proskaurer………………….. - - -
Simmons Citrate…………………....... V V +
Malanote……….……………………….. - + +
Gluconate……………………………….. - - +
Christensen’s urease……………..... V V V
Motility…………………………………... - + +
-
Gelatin liquefaction………………… V - +
KCN broth-growth………………….. V - +
Arginin dihydrolase………………… - - +
Lysine decarboxylase……………… - - -
Ornithine decarboxylase…………. - - -
Phenylalanine deaminase……….. - +- -
Key characteristic
Pclliclc (serum) top of liquid mcda - - +
KIA/TSIA : metallic sheenon slant - - +
Pigmentation………………………….. - - V+5
Odor……………………………………..... - - 6
10
Mac Concey Agar………………….…. V G G
1
Formerly classified as TribeMima Mima polymorpha and Herellea vaginicola.
2
Moroxellaosleonsis formerly classified polymorpha van oxodans; oxidase positive
3
Proposed subdivision of Acinetobacter; A. anitratum (formerly H. vaginicola), oxidative (O);
7
A (formerly M. polymorpha), inert (-) Strong
4 8
Acinetobacter anitratum Inert
5 9
Blue-green Rapid
6 10
Grape-like Slight