Experiment No.

1
SOLUBILITY DIFFERENCES

LABORATORY REPORT

Name: Rose Ann A. Aguirre Date: October 5, 2021

Objectives: To observe and to know the solubility of water and organic solvents
against different substances.

_____________________________________________________________________
Materials Needed:
Quantity Apparatus Quantity Chemicals
9 Test tube 1 mL Carbon tetrachloride
1 Test tube rack 1 mL 2-Butanol
1 Dropper 1 mL Glacial Acetic Acid
1 Beaker 1 mL Benzoic Acid
1 Graduated Cylinder 1 mL Cooking oil
1 Hot plate Small amount Naphthalene
1 Small amount Styrofoam
Serological Pipette
1 Small amount Sugar
Bulb syringe
1 Small amount NaCl
Spatula
Water
OBSERVATIONS and RESULTS

I. Solubility Differences in Water and Chloroform

DESCRIPTION OF MIXTURE WITH
SAMPLE COMPUNDS
Water as Solvent Carbon Tetrachloride as Solvent
It dissolves easily in It dissolve easily after being
Chloroform
water and it have a agitate and the color is
CHCI3
transparent color. transparent also.
2-Butanol dissolved
in cold water and it
Butanol dissolved together with
is colorless. While
Carbon Tetrachloride and the
2-Butanol in hot water, it is
color of the combine mixture
C4H2OH like a carbonated
after being agitate is cloudy
soft drink because
white.
it has a cloudy
white color.

DESCRIPTION OF MIXTURE WITH

SAMPLE COMPUNDS
Water as Solvent Carbon Tetrachloride as
Solvent
Acetic acid and carbon
In cold and hot water,
Tetrachloride was being
Acetic Acid glacial acetic acid was
combine dissolved
CH3COOH completely blend and
together. The color
dissolve into one another.
becomes cloudy white.
Benzoic acid and Carbon
Benzoic acid slightly
Tetrachloride dissolved
Benzoic Acid dissolved in cold water. It
together and the color
C6H5COOH completely dissolved when
becomes transparent or
heated.
clear after being agitate.
Naphthalene Naphthalene dissolved in The naphthalene dissolved

C10H8
Sodium Chloride NaCl
II. Salting-out effect
water. I think there’s a
bubbles and some of the
together with Carbon
mixture that not dissolved
Tetrachloride. The color
lift up. In hot water, the
becomes transparent.
particles of naphthalene
floats on the top.
In cold and hot water,
The solid particles of NACl
NaCl dissolved after being
now can be shown.
agitate.

DESCRIPTION OF MIXTURE AFTER ADDITION OF NaCI

DESCRIPTION OF
ORIGINAL MIXTURE
(2-Butanol & Water) Immediate Appearance
The 3 mixture dissolved
easily after being agitate
and the color became
cloudy white
Appearance after 30
minutes
After 30 minutes, some
particles of salt can be rise
up or can seen in the top
of the mixture.

Question:

1. Which of the organic compound did not dissolve in water but dissolve in
chloroform? Explain why.

Styrofoam did not dissolved in water but it dissolves in chloroform because both
of them is non polar solvent. Styrofoam is made from polystyrene and foam. Due
to their similar polarities, it can dissolve the carbon-hydrogen bonds of
Styrofoam.

2.What is the role of NaCI in salting-out effect?

The role of NaCl in salting-out effect is to decrease the solubility of protein. Ehen
we add salt, the charged ions interact with water. This means that there are less
solvent water molecules to interact with the protein. As a result, protein
molecules begin to aggregate, which cause precipitation.