Pasteur’s

Experimentation
GROUP 3 | LEADER: Marie Raphaelle Doydoy
REPORTERS: Marie Raphaelle Doydoy, Jennylyn Narisma | RESEARCHERS: Jethro Oquendo, Ksiah Mae Plasus,
Yuan Geronimo, Naeomi Jane Naz, Angelyn Ocsebio | PRESENTATION: Mark Renzo Salazar
 Pasteur’s
Introduction Louis Pasteur Group 3
Experimentation

A. Topic/ How does science help us

Title explain all life came
to be?
B. Proponents The topic/title of this report is “The
Pasteur Experimentation Theory”,
b
wherein we are going to tackle about how
life was formed in this world according to
Louis Pasteur’s experiment back 1862.

C. Leading
Questions

D. Content/
 Pasteur’s
Introduction Louis Pasteur Group 3
Experimentation

B. Proponents How does science help us

explain all life came
to be?
C. Leading The topic/title of this report is “The
Questions Pasteur Experimentation Theory”,
wherein we are going to tackle about how
life was formed in this world according to
Louis Pasteur’s experiment back 1862.

D. Content/ The proponents of this theory is

Details Louis Pasteur.

E. Reference
 Pasteur’s
Experimentation

Louis Pasteur’s Swan Neck Flask

Experiment
WHY DID HE MAKE THIS
EXPERIMENT?
During the 18th century, the existence of “spontaneous
phenomena” was a huge debate among different
experts. Some argued and supported the concept of
spontaneous generation, whereas some also
contrasted against this belief; life cannot generate
from dead matter. In 1859, Charles Darwin published
his own writings about this matter, so Louis Pasteur
decided to settle such A dispute. He experimented to
attest the validity of a widely held belief in
spontaneous generation, that a variety of organisms
could arise spontaneously, without being generated
from similar, parental organisms. Thus, this is a
definitive experiment to know whether organisms can
attain life by non-living matter.
WHAT IS SPONTANEOUS
GENERATION?
Spontaneous generation is an obsolete body of
thought on the ordinary formation of living organisms
without descent from similar organisms. Typically, the
idea was that certain forms such as fleas could arise
from inanimate matter such as dust or that maggots
could arise from dead flesh. A variant idea was that of
equivocal generation, in which species such as
tapeworms arose from unrelated living organisms,
now understood to be their hosts.

Pasteur’s
Experimentation
Q1
What was different about the two
flasks that Pasteur used?
Q2
Why did Pasteur boil the broth?
Q3
What did Pasteur find when he
checked the flasks after several days?
Q4
Why did the straight necked flask
grow microorganisms?
Earth and Life Science
C. Leading Questions (based on the topic;
imposes very important question/s; it
should be appealing to the audience such
that they would be encouraged to read
through) Q5
Why is there no growth of
microorganisms in s-neck flask?
Q6
How did Pasteur’s experiment
disprove spontaneous generation?
Q7
What was Pasteur’s conclusion?
group 3
D. Content/ details (answers to questions and facts group 3
provided)

Question #1

He left one flask with a straight neck. The other he bent to

form an "S" shape.
D. Content/ details (answers to questions and facts group 3
provided)

Question #2

Based on Pasteur's observation, he already knew that

bacteria grow in open containers of meat broth and if the
broth is boiled for an hour in a sealed container, no
bacteria will grow on it. He boiled the broth to test if
microbes arise from pre-existing microbes or are
generated spontaneously.
D. Content/ details (answers to questions and facts group 3
provided)

Question #3

After several days, Pasteur found out that the broth in the
straight-neck flask was discolored and cloudy, while the
broth in the curved-neck flask did not change. Therefore,
Pasteur concluded that bacterias in the air were able to fall
in the straight-neck flask and it contaminated the broth.
While the curved-neck flask trapped the bacterias in its
curved neck and prevented it from reaching the broth.
That's why it never changed color or became cloudy.
D. Content/ details (answers to questions and facts group 3
provided)

Question #4

Based on the experiment, dust particles from the air fell

into the broken flask, but dust particles remained near the
tip of the swan necks in the intact flask. They weren’t able
to travel against gravity into the flasks. Once the broth was
broken, the flasks became cloudy because it had microbial
life. On the other hand, the broth in the unbroken flask
remained clear. Without the dust inside the flasks, the
microbes cannot travel. So no life arose.
D. Content/ details (answers to questions and facts group 3
provided)

Question #5

The swan neck significantly slows down the motion of air

through the tube, and particles in the air, such as bacteria,
become trapped on moisture on its inner surfaces. As a
result, sterile liquid in the vessel itself remains sterile as
long as the liquid does not contact the contaminated liquid
in the tube.
D. Content/ details (answers to questions and facts group 3
provided)

Question #6
In the mid-19th century, there was an idea that any microbial life
could automatically spawn in any liquids. This was known as
spontaneous generation. Louis Pasteur ultimately disproved this,
demonstrating that microbes only came from other microbes;
contamination caused microbes to appear in liquid, not
spontaneous generation. The archaic hypothesis that used this
mechanism to explain the genesis of life; also, the hypothesized
process by which living beings develop from nonliving substance.
Pieces of cheese and bread wrapped in rags and placed in a dark
corner, for example, were considered to create mice since mice
were found in the rags after several weeks, according to that notion.
Many people believed in spontaneous generation because it
explained phenomena like maggots on decaying meat.
D. Content/ details (answers to questions and facts group 3
provided)

Question #6
By the 18th century, it had become clear that nonliving matter could
not form higher species. However, the genesis of microbes like
bacteria was not entirely understood.

Until Louis Pasteur established that bacteria reproduced in the 19th

century. Also see biopoiesis.
D. Content/ details (answers to questions and facts group 3
provided)

Question #7

Pasteur’s set of experiments irrefutably disproved the theory of

spontaneous generation and earned him the prestigious Alhumbert
Prize from the Paris Academy of Sciences in 1862. In a subsequent
lecture in 1864, Pasteur articulated “Omne vivum ex vivo” (“Life only
comes from life”). In this lecture, Pasteur recounted his famous
swan-neck flask experiment, stating that “life is a germ and a germ
is life. Never will the doctrine of spontaneous generation recover
from the mortal blow of this simple experiment.” To Pasteur’s credit,
it never has. Pasteur's experiment showed that microbes cannot
arise from nonliving materials under the conditions that existed on
Earth during his lifetime. But his experiment did not prove that
spontaneous generation never occurred.
 Pasteur’s
Earth and Life group 3
Experimentation
Sccience

REFERENCES
Louis Pasteur’s Swan Neck Flask
1. Britannica-
https://www.britannica.com/biography/Louis-
Pasteur/Spontaneous-generation
2. Script-
http://bcs.whfreeman.com/webpub/biology/sadavalife9
e/animated%20tutorials/life9e_0401_script.html
Video Example
Youtube-
https://www.youtube.com/watch?v=xmyBtrqC-rw
Questions 1
How Stuff Works-
https://science.howstuffworks.com/innovation/scientifi
c-experiments/scientific-method5.html
Questions 2 Question 6
Script- Study.com-
http://bcs.whfreeman.com/webpub/biology/sadavalife9 https://study.com/academy/answer/how-did-louis-
e/animated%20tutorials/life9e_0401_script.html pasteur-disprove-spontaneous-generation.html
Britannica-
https://www.britannica.com/biography/Louis-
Pasteur/Spontaneous-generation
Questions 3, 4, 5
3. How Stuff Works-
https://science.howstuffworks.com/innovation/scientifi Question 7
c-experiments/scientific-method5.html
Lumen Learning-
4. Script- https://courses.lumenlearning.com/microbiology/chapt
http://bcs.whfreeman.com/webpub/biology/sadavalife9 er/spontaneous-generation/
e/animated%20tutorials/life9e_0401_script.html
Script-
http://bcs.whfreeman.com/webpub/biology/sadavalife9
5. Wikipedia-
https://en.wikipedia.org/wiki/Swan_neck_flask e/animated%20tutorials/life9e_0401_script.html
 End of Presentation

GROUP 3 | LEADER: Marie Raphaelle Doydoy

REPORTERS: Marie Raphaelle Doydoy, Jennylyn Narisma | RESEARCHERS: Jethro Oquendo, Ksiah Mae Plasus,
Yuan Geronimo, Naeomi Jane Naz, Angelyn Ocsebio | PRESENTATION: Mark Renzo Salazar