Experiment 7: LIPID HYDROLYSIS

Score:

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Last name Given name MI

Course/Year Date performed Date

submitted

Laboratory class schedule Laboratory Instructor/Professor

POST-LAB ACTIVITY
RESULTS

A. Solubility of Lipid in different solvents.

Please watch the video link for the experiment procedure.
https://youtu.be/IgMopgqnWqo

Table 1. Solubility of lipid in different solvents.

Solvents Observation
Water
Ethanol
Diethyl Ether
CHCl3

Exploratory questions:

1. Based on the experiment, explain the solubility of the lipid in different solvents. Why they are
soluble or not?

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 B. Effect of Bile Salts on Oil

Please watch the video link for the experiment procedure. https://youtu.be/3DlJHXyqWUs

Table 2. Effect of bile salts on peanut oil

Sample Observation
d.H2O + peanut oil
Bile salt solution +
peanut oil

Exploratory questions:

2. What happens to the peanut oil in the presence of bile? What is the role of bile in lipid
hydrolysis? Explain.

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C. Fat Digestion by Pancreatin and Bile

Please watch the video link for the experiment procedure. https://youtu.be/1rRzufWI2D8

Table 3. Fat Digestion by Pancreatin and Bile

Test Tube Contain in the

Observation
Label Test Tube
1L
2L
3L
4L
5L
4B
5B
Exploratory questions:

3. What is the role of pancreatin and bile in fat digestion? Explain. What other enzyme/s is/are
responsible for the fat digestion? Why do you need to add litmus cream? Write the chemical
reaction that occur in this experiment (4L,4B, 5L,& 5B)

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REFERENCES:

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