Name :

Id :
LAB REPORT
CHROMATOGRAPHY

Video Link

https://www.youtube.com/watch?v=NSJIAcfvEJI

Objectives

 To separate the components from the red and blue ink mixture
 To determine the value of Rf of red and blue inks

Material and Apparatuses

 Chromatographic chamber with lid

 Mixture of red and blue inks
 Isopropyl alcohol-water mixture
 Whatman filter paper strip
 Fine capillary tube
 Ruler
 Thread
 Pencil

Background theory

Procedures

1. Take the whatman filter paper strip and draw a line with a pencil above 2 cm from
one end

2. Draw another line lengthwise from the center of the paper

3. Name the point at which the two lines intersect as P

4. Take the mixture of red and blue inks using capillary tube

5. Put a drop of mixture of red and blue inks at the point P

6. Allow it to dry in open air

7. Put another drop on the same spot and dry again, so that the spot rich with the mixture

8. Take a piece of thread and tie the filter paper with it

 9. Suspend the filter paper vertically in the chromatographic chamber containing mixture
of isopropyl alcohol and distilled water

10. Make sure the pencil line remains about 1 cm above the solvent

11. Keep the chamber undisturbed for some time.

12. Notice the rising solvent along with the red and blue inks

13. After the solvent has risen, you will notice two different spot of blue and red colors on
the filter paper

14. Take the filter paper out of the chamber and mark the distance that the solvent has
risen on the paper with a pencil (the solvent front)

15. Dry the filter paper and put pencil marks on the center of the red and blue spot

16. Measure the distance of the two spot from the original line and the distance of the
solvent from the original line

17. Calculate the Rf values of red and blue inks.

Data and Result

The data :

The distance of red spot = 15 mm

The distance of blue spot = 39 mm

The distance of solvent =47 mm

Calculate the Rf using this formula

spot distance
Rf value=
solvent distance

Rf value=¿

Discussion

1. What is the main advantage on of using chromatography

2. Why do we cover the chromatographic chamber with a lid
3. Why do we need to mark the solvent front?

Conclusion
Name :

Id :
LAB REPORT
SIMPLE DISTILLATION

Video Link

https://www.youtube.com/watch?v=HIsSqMMVPnQ

Objectives

To separate a mixture using distillation process (CuSO4 with distilled water)

Material and Apparatuses

 Distilling flask  Spiritus burner

 Distilling head  2 Stand and clip
 Thermometer  Tripod
 Thermometer adapter  Wire gauze with asbestos center
 Boiling chips  Graduated cylinder
 Condenser  Unknown sample
 Rubber tubing  Container
 Adapter  Procedure
 Erlenmeyer flask as receiver  Water
Background theory

Procedures

1. Fill the container with the water

2. Place 100 mL of unknown sample into distillation flask

3. Add 2 boiling chips into distillation flask

4. Note the temperature

5. Set all the distillation apparatuses

6. Make sure the rubber tubing work well, also avoid folds to make the water flows

7. Start the heating process and note the initial temperature

8. Observe the increasing of temperature for each minutes

9. Note the temperature regarding volume distilled

 10. Note the constant temperature

11. Wait until the pure water goes down into Erlenmeyer flask

12. Collect the volume of distilled water.

Data and Result

Minute
Temperatur
e

Minute
Temperatur
e

Minute
Temperatur
e

The temperature when the first distilled water rise =

The final volume of distilled water =

Discussion

1. From the observation table what can you conclude about the purity of the distilled
water?
2. Can simple distillation be used to separate liquids with very close boiling point?, give
the reason?

Conclusion