Test Protocol for CA-500 -(1) System software version 00-09

PT (Thromborel® S) PT (Thromboplastin•C Plus)

Detector Clot (PT) Detector Clot (PT)
End Point 50 % End Point 50 %
Maximum Time 120 sec Maximum Time 100 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 50 µL Sample Vol. 50 µL

Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 180 sec Reagent 1 180 sec
Reag. Vol. PT THS 100 µL Reag. Vol. PT TPC+ 100 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 0 sec Reagent 2 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
WARNING WARNING
Please carry out reagent selection. Please carry out reagent selection.
(Service engineer will be able to change this setting) (Service engineer will be able to change this setting)

PT (INNOVIN) PT (Pathromtin® SL)

Detector Clot (PT) Detector Clot (APTT)
End Point 50 % End Point 50 %
Maximum Time 100 sec Maximum Time 190 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 50 µL Sample Vol. 50 µL

Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 180 sec Reagent 1 60 sec
Reag. Vol. PT INN 100 µL Reag. Vol. APTT PSL 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 0 sec Reagent 2 180 sec
Reag. Vol. ******* 0 µL Reag. Vol. CaCl2 50 µL
Deter. ******* x0 Deter. Rinse x1
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
WARNING WARNING
Please carry out reagent selection. Please carry out reagent selection.
(Service engineer will be able to change this setting) (Service engineer will be able to change this setting)
Test Protocol for CA-500 -(2) System software version 00-09

APTT (ACTIN® ) APTT (ACTIN® FS)

Detector Clot (APTT) Detector Clot (APTT)
End Point 50 % End Point 50 %
Maximum Time 190 sec Maximum Time 190 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 50 µL Sample Vol. 50 µL

Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 60 sec Reagent 1 60 sec
Reag. Vol. APTT ACT 50 µL Reag. Vol. APTT FS 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 240 sec Reagent 2 240 sec
Reag. Vol. CaCl2 50 µL Reag. Vol. CaCl2 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
WARNING WARNING
Please carry out reagent selection. Please carry out reagent selection.
(Service engineer will be able to change this setting) (Service engineer will be able to change this setting)

APTT (ACTIN® FSL) Fbg (Fibrinogen Determination Reagent)

Detector Clot (APTT) Detector Clot (Fbg)
End Point 50 % End Point 50 %
Maximum Time 190 sec Maximum Time 100 sec
Sensitivity Low Gain Sensitivity High Gain

Sample Vol. 50 µL Sample Vol. 10 µL

Dil. Vol. ******* 0 µL Dil. Vol. OVB 90 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 60 sec Reagent 1 180 sec
Reag. Vol. APTT FSL 50 µL Reag. Vol. Fbg 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 240 sec Reagent 2 0 sec
Reag. Vol. CaCl2 50 µL Reag. Vol. ******* 0 µL
Deter. Rinse x1 Deter. ******* x0
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
WARNING
Please carry out reagent selection.
(Service engineer will be able to change this setting)
Test Protocol for CA-500 -(3) System software version 00-09

Fbg (Mulitifibrin® U ) TT (Test Thrombin Reagent)

Detector Clot (Fbg) Detector Clot (TT)
End Point 50 % End Point 50 %
Maximum Time 200 sec Maximum Time 150 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 70 µL Sample Vol. 50 µL

Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 60 sec Reagent 1 60 sec
Reag. Vol. Fbg MFU 100 µL Reag. Vol. Test-Thr 100 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 0 sec Reagent 2 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0

TT (Thromboclotin®) Batroxobin Time (Batroxobin Reagent)

Detector Clot (TT) Detector Clot (TT)
End Point 50 % End Point 50 %
Maximum Time 150 sec Maximum Time 150 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 100 µL Sample Vol. 50 µL

Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 60 sec Reagent 1 60 sec
Reag. Vol. Thromboc 100 µL Reag. Vol. Batrox 100 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 0 sec Reagent 2 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
Test Protocol for CA-500 -(4) System software version 00-09

Extrinsic Factors (II, V, VII, X) Intrinsic Factors (VIII, IX, XI, XII)
Detector Clot (Other) Detector Clot (Other)
End Point 50 % End Point 50 %
Maximum Time 180 sec Maximum Time 180 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 5 µL Sample Vol. 5 µL

Dil. Vol. OVB 45 µL Dil. Vol. OVB 45 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 30 sec Reagent 1 30 sec
Reag. Vol. Factor DP 50 µL Reag. Vol. Factor DP 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 210 sec Reagent 2 90 sec
Reag. Vol. PT Reag 100 µL Reag. Vol. APTT Reag 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 3 0 sec Reagent 3 270 sec
Reag. Vol. ******* 0 µL Reag. Vol. CaCl2 50 µL
Deter. ******* x0 Deter. Rinse x1

PS Coagrometric (Protein S Reagent) PC Coagrometric (Protein C Reagent)

Detector Clot (other) Detector Clot (other)
End Point 50 % End Point 50 %
Maximum Time 400 sec Maximum Time 300 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 5 µL Sample Vol. 5 µL

Dil. Vol. PS DP 25 µL Dil. Vol. PC.DP 45 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 0 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 60 sec Reagent 1 30 sec
Reag. Vol. PS.APC 40 µL Reag. Vol. PC.Act 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 240 sec Reagent 2 60 sec
Reag. Vol. PS.FVa. 125 µL Reag. Vol. Neothr 50 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 3 0 sec Reagent 3 300 sec
Reag. Vol. ******* 0 µL Reag. Vol. CaCl2 50 µL
Deter. ******* x0 Deter. Rinse x1

WARNING WARNING
Protein S Deficient Plasma set at Diluent Holder Protein C Deficient Plasma set at Diluent Holder
CLICK CLICK
Test Protocol for CA-500 -(5) System software version 00-09

AT III (Berichrom® Antithrombin III (A)) APL (Berichrom® α2-Antitplasmin)

Detector Chrom. Detector Chrom.
Start Point 11 sec Start Point 42 sec
End Point 40 sec End Point 72 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 15 µL Sample Vol. 16 µL

Dil. Vol. OVB 90 µL Dil. Vol. OVB 112 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 20 µL D. Samp Vol. 20 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 30 sec Reagent 1 30 sec
Reag. Vol. AT3Thro 125 µL Reag. Vol. AplReag 125 µL
Deter. Rinse x1 Deter. Clean II x1
Reagent 2 210 sec Reagent 2 90 sec
Reag. Vol. AT3Subs 33 µL Reag. Vol. AplSubs 25 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0

PLG (Berichrom® Plasminogen) PC (Berichrom® Protein C)

Detector Chrom. Detector Chrom.
Start Point 42 sec Start Point 11 sec
End Point 90 sec End Point 100 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 16 µL Sample Vol. 20 µL

Dil. Vol. OVB 112 µL Dil. Vol. ******* 0 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 20 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 30 sec Reagent 1 60 sec
Reag. Vol. Strepto 125 µL Reag. Vol. BCPCAct 125 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 330 sec Reagent 2 540 sec
Reag. Vol. PlgSubs 25 µL Reag. Vol. BCPCSub 30 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 3 0 sec Reagent 3 0 sec
Reag. Vol. ******* 0 µL Reag. Vol. ******* 0 µL
Deter. ******* x0 Deter. ******* x0
Test Protocol for CA-500 -(6) System software version 00-09

C1-INH (Berichrom® C1-Inhibitor) Heparin (Berichrom® Heparin)

Detector Chrom. Detector Chrom.
Start Point 10 sec Start Point 10 sec
End Point 70 sec End Point 70 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 15 µL Sample Vol. 10 µL

Dil. Vol. OVB 60 µL Dil. Vol. OVB 10 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 20 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 30 sec Reagent 1 30 sec
Reag. Vol. C1Ester 80 µL Reag. Vol. AT3Reag 20 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 60 sec Reagent 2 60 sec
Reag. Vol. C1Ester 80 µL Reag. Vol. FXaReag 100 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 3 330 sec Reagent 3 120 sec
Reag. Vol. C1Subs 20 µL Reag. Vol. HepSubs 20 µL
Deter. Rinse x1 Deter. Rinse x1

WARNING
AT III Reagent dilute 1:2 with Owren's veronal buffer
before use.

FM (Berichrom® FM) PAI (Berichrom® PAI)

Detector Chrom. Detector Chrom.
Start Point 42 sec Start Point 40 sec
End Point 90 sec End Point 90 sec
Sensitivity Low Gain Sensitivity Low Gain

Sample Vol. 16 µL Sample Vol. 10 µL

Dil. Vol. OVB 112 µL Dil. Vol. PAI Uro 20 µL
2nd. Dil. 2nd. Dil.
D. Samp Vol. 20 µL D. Samp Vol. 0 µL
Dil. Vol. ******* 0 µL Dil. Vol. ******* 0 µL
Reagent 1 30 sec Reagent 1 300 sec
Reag. Vol. FM Plg 125 µL Reag. Vol. PAI Plg 40 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 2 330 sec Reagent 2 320 sec
Reag. Vol. FM tPA 25 µL Reag. Vol. PAI Oxi 40 µL
Deter. Rinse x1 Deter. Rinse x1
Reagent 3 330 sec Reagent 3 620 sec
Reag. Vol. FM Subs 25 µL Reag. Vol. PAI Sub 100 µL
Deter. Rinse x1 Deter. Rinse x1

WARNING
Urokinase Reagent set at Diluent Holder
CLICK